Estrogen Receptor Beta 5 antibody | 5/25
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|Mouse anti human estrogen receptor beta 5, clone 5/25 recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ERbeta (ESR2), one of at least five isoforms (Moore et al. 1998). Although only the ERB1 variant appears fully functional, the remaining isoforms have been shown to form heterodimers with ERB1, thereby enhancing transcription (Shaaban et al. 2008).
ERB5 is expressed in normal colorectal tissue, and is present in primary colorectal carcinomas (Wong et al. 2005).
The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formal fixed paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy transcription (Shaaban et al. 2008, Collins et al. 2009).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Tuberculin conjugated synthetic peptide LLSHVRHARYAP derived from the C-terminus of human ERB5.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Normal colon or colon carcinoma
References for Estrogen Receptor Beta 5 antibody
Wong, N.A. et al. (2005) ERbeta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates.
J Pathol. 207 (1): 53-60.
Shaaban, A.M. et al. (2008) Nuclear and cytoplasmic expression of ERbeta1, ERbeta2, and ERbeta5 identifies distinct prognostic outcome for breast cancer patients.
Clin Cancer Res. 14 (16): 5228-35.
Collins, F. et al. (2009) Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.
BMC Cancer. 9: 330.
Ciucci, A. et al. (2014) Gender effect in experimental models of human medulloblastoma: does the estrogen receptor β signaling play a role?
PLoS One. 9 (7): e101623.
Zannoni, G.F. et al. (2016) Sexual dimorphism in medulloblastoma features.
Histopathology. 68 (4): 541-8.
Li, S.Y. et al. (2015) Cytoplasm estrogen receptor β5 as an improved prognostic factor in thymoma and thymic carcinoma progression.
Oncol Lett. 10 (4): 2341-6.
Ciucci, A. et al. (2018) Estrogen receptor β: Potential target for therapy in adult granulosa cell tumors?
Gynecol Oncol. 150 (1): 158-65.
Bai, Y. et al. (2018) Oestrogen receptor β5 and epidermal growth factor receptor synergistically promote lung cancer progression.
Autoimmunity. 51 (4): 157-165.
View The Latest Product References
Wang, M. et al. (2019) The alteration of ERβ5 and collagen metabolism is relevant to the development of stress urinary incontinence
Int J Clin Exp Med 12(5): 5154-61.
Ciucci, A. et al. (2014) Prognostic significance of the estrogen receptor beta (ERβ) isoforms ERβ1, ERβ2, and ERβ5 in advanced serous ovarian cancer.
Gynecol Oncol. 132 (2): 351-9.
Younes, M. et al. (2018) Expression of estrogen receptor beta isoforms in pancreatic adenocarcinoma.
Oncotarget. 9 (102): 37715-20.
Hexiao, T. et al. (2021) Knockdown of CENPF inhibits the progression of lung adenocarcinoma mediated by ERβ2/5 pathway.
Aging (Albany NY). 13 (2): 2604-25.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"View all Anti-Human Products
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