Estrogen Receptor Beta 5 antibody | 5/25
Mouse anti Human estrogen receptor β5 antibody, clone 5/25 (MCA4676GA) used for the detection of ERβ5 expressing cells in human endometrial cancer tissue biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. All proteins were immunolocalised to cell nuclei (see higher power inserts in panels e, f, c and d respectively). In the well and moderately differentiated cancers expression was most intense in epithelial cell layers (arrowheads, panel A and inserts). Note that ERα was low/absent in poor grade cancers (J) but immunoexpression of ERβ1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERβ1, ERβ2 and ERβ5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers.
From: Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, Jabbour HN, Saunders PT.
Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.
BMC Cancer. 2009 Sep 16;9:330.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human estrogen receptor β5 antibody, clone 5/25 (MCA4676GA) used for the detection of ERβ5 expressing cells in tumors by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Effect of gender on ERβ1, ERβ2 and ERβ5 expression in D283 Med xenografts. A) Immunostaining for ERβ1 was significantly higher in tumors from females compared to males (**p<0.01); B) and C) Tumor levels of ERβ2 and ERβ5 did not significantly differ between females and males. All results are expressed as the mean ± SEM (n = 6 tumors/group). Representative stained section of tumors from females and males are shown (magnification 40x). IHC, immunohistochemical score.
From:Ciucci A, Meco D, De Stefano I, Travaglia D, Zannoni GF, et al. (2014)
Gender Effect in Experimental Models of Human Medulloblastoma: Does the Estrogen Receptor β Signaling Play a Role?
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human estrogen receptor β5 antibody, clone 5/25 (MCA4676GA) used for the detection of ERβ5 expressing cells in tumors by immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Detail of image C.
Image caption:
Effect of gender on ERβ1, ERβ2 and ERβ5 expression in D283 Med xenografts. A) Immunostaining for ERβ5 did not significantly differ between females and males. All results are expressed as the mean ± SEM (n = 6 tumors/group). Representative stained section of tumors from females and males are shown (magnification 40x). IHC, immunohistochemical score.
From:Ciucci A, Meco D, De Stefano I, Travaglia D, Zannoni GF, et al. (2014)
Gender Effect in Experimental Models of Human Medulloblastoma: Does the Estrogen Receptor β Signaling Play a Role?
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human estrogen receptor β5 antibody, clone 5/25 (MCA4676GA) used for the detection of ERβ5 expressing cells in normal human liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Staining pattern of the ERβ isoforms in human liver. In order to confirm the specificity of the ERβ antibodies used in the study, we immunostained sections of human healthy liver, showing that ERβ1 and ERβ2 isoforms are not expressed, while specific nuclear ERβ5 immunoreactivity is detected (magnification 20x and 40x). This pattern of ERβ isoforms immunoreactivity is consistent with previous descriptions.
From:Ciucci A, Meco D, De Stefano I, Travaglia D, Zannoni GF, et al. (2014)
Gender Effect in Experimental Models of Human Medulloblastoma: Does the Estrogen Receptor β Signaling Play a Role?
PLoS ONE 9(7): e101623.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti human estrogen receptor beta 5, clone 5/25 (MCA4676GA) used to demonstrate ERβ5 expression an adenocarcinoma tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Comparison with ERβ1 (MCA1974) and ERβ2 (MCA2279).
Image caption:
Examples of positive immunohistochemical staining for estrogen receptor (ER)-beta1 (A), ER-beta2 (B) and ER-beta5 (C) in sections of human pancreatic adenocarcinoma tissue. 20× microscope objective.
From:Younes M, Ly CJ, Singh K, Ertan A, Younes PS, Bailey JM.
Expression of estrogen receptor beta isoforms in pancreatic adenocarcinoma.
Oncotarget. 2018 Dec 28;9(102):37715-20.
doi: 10.18632/oncotarget.26503.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Estrogen receptor beta 5 antibody, clone 5/25 (MCA4676GA) used to identify ERβ5 expressing cells in murine tumors by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Knockdown of CENPF can inhibit ERβ2/5 pathway-mediated tumor tissue growth in vivo. Immunohistochemical analysis of the expression of CENPF, ERβ, ERβ2 and ERβ5 in nude mice tumor tissues.
From: Hexiao T, Yuquan B, Lecai X, Yanhong W, Li S, Weidong H, Ming X, Xuefeng Z, Gaofeng P, Li Z, Minglin Z, Zheng T, Zetian Y, Xiao Z, Yi C, Lanuti M, Jinping Z.
Knockdown of CENPF inhibits the progression of lung adenocarcinoma mediated by ERβ2/5 pathway.
Aging (Albany NY). 2021 Jan 10;13(2):2604-25.
doi: 10.18632/aging.202303.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Estrogen Receptor Beta 5
- Product Type
- Monoclonal Antibody
- Clone
- 5/25
- Isotype
- IgG1
- Specificity
- Estrogen Receptor Beta 5
Antibody Quality and Performance Guarantee
| Mouse anti human estrogen receptor beta 5, clone 5/25 recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ERbeta (ESR2), one of at least five isoforms (Moore et al. 1998). Although only the ERB1 variant appears fully functional, the remaining isoforms have been shown to form heterodimers with ERB1, thereby enhancing transcription (Shaaban et al. 2008). ERB5 is expressed in normal colorectal tissue, and is present in primary colorectal carcinomas (Wong et al. 2005). The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formal fixed paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy transcription (Shaaban et al. 2008, Collins et al. 2009). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Tuberculin conjugated synthetic peptide LLSHVRHARYAP derived from the C-terminus of human ERB5.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
||
| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Normal colon or colon carcinoma
References for Estrogen Receptor Beta 5 antibody
-
Wong, N.A. et al. (2005) ERbeta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates.
J Pathol. 207 (1): 53-60. -
Shaaban, A.M. et al. (2008) Nuclear and cytoplasmic expression of ERbeta1, ERbeta2, and ERbeta5 identifies distinct prognostic outcome for breast cancer patients.
Clin Cancer Res. 14 (16): 5228-35. -
Collins, F. et al. (2009) Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.
BMC Cancer. 9: 330. -
Ciucci, A. et al. (2014) Gender effect in experimental models of human medulloblastoma: does the estrogen receptor β signaling play a role?
PLoS One. 9 (7): e101623. -
Ciucci, A. et al. (2014) Prognostic significance of the estrogen receptor beta (ERβ) isoforms ERβ1, ERβ2, and ERβ5 in advanced serous ovarian cancer.
Gynecol Oncol. 132 (2): 351-9. -
Li, S.Y. et al. (2015) Cytoplasm estrogen receptor β5 as an improved prognostic factor in thymoma and thymic carcinoma progression.
Oncol Lett. 10 (4): 2341-6. -
Zannoni, G.F. et al. (2016) Sexual dimorphism in medulloblastoma features.
Histopathology. 68 (4): 541-8. -
Ciucci, A. et al. (2018) Estrogen receptor β: Potential target for therapy in adult granulosa cell tumors?
Gynecol Oncol. 150 (1): 158-65.
View The Latest Product References
-
Bai, Y. et al. (2018) Oestrogen receptor β5 and epidermal growth factor receptor synergistically promote lung cancer progression.
Autoimmunity. 51 (4): 157-165. -
Younes, M. et al. (2018) Expression of estrogen receptor beta isoforms in pancreatic adenocarcinoma.
Oncotarget. 9 (102): 37715-20. -
Wang, M. et al. (2019) The alteration of ERβ5 and collagen metabolism is relevant to the development of stress urinary incontinence
Int J Clin Exp Med 12(5): 5154-61. -
Hexiao, T. et al. (2021) Knockdown of CENPF inhibits the progression of lung adenocarcinoma mediated by ERβ2/5 pathway.
Aging (Albany NY). 13 (2): 2604-25.
- RRID
- AB_1604962
- UniProt
- Q92731
- Entrez Gene
- ESR2
MCA4676T
171026
180510
MCA4676GA
154673
180510
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