Estrogen Receptor Beta 5 antibody | 5/25

Mouse anti Human Estrogen Receptor Beta 5

Product Type
Monoclonal Antibody
Clone
5/25
Isotype
IgG1
Specificity
Estrogen Receptor Beta 5

Product Code Applications Pack Size List Price Your Price Qty
MCA4676GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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loader
MCA4676T
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SDS Safety Datasheet SDS
P * WB 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti human estrogen receptor beta 5, clone 5/25 recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ERbeta (ESR2), one of at least five isoforms (Moore et al. 1998). Although only the ERB1 variant appears fully functional, the remaining isoforms have been shown to form heterodimers with ERB1, thereby enhancing transcription (Shaaban et al. 2008).

ERB5 is expressed in normal colorectal tissue, and is present in primary colorectal carcinomas (Wong et al. 2005).

The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formal fixed paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy transcription (Shaaban et al. 2008, Collins et al. 2009).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Tuberculin conjugated synthetic peptide LLSHVRHARYAP derived from the C-terminus of human ERB5.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Normal colon or colon carcinoma

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References for Estrogen Receptor Beta 5 antibody

  1. Wong, N.A. et al. (2005) ERbeta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates.
    J Pathol. 207 (1): 53-60.
  2. Shaaban, A.M. et al. (2008) Nuclear and cytoplasmic expression of ERbeta1, ERbeta2, and ERbeta5 identifies distinct prognostic outcome for breast cancer patients.
    Clin Cancer Res. 14 (16): 5228-35.
  3. Collins, F. et al. (2009) Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.
    BMC Cancer. 9: 330.
  4. Ciucci, A. et al. (2014) Gender effect in experimental models of human medulloblastoma: does the estrogen receptor β signaling play a role?
    PLoS One. 9 (7): e101623.
  5. Zannoni, G.F. et al. (2016) Sexual dimorphism in medulloblastoma features.
    Histopathology. 68 (4): 541-8.
  6. Li, S.Y. et al. (2015) Cytoplasm estrogen receptor β5 as an improved prognostic factor in thymoma and thymic carcinoma progression.
    Oncol Lett. 10 (4): 2341-6.
  7. Ciucci, A. et al. (2018) Estrogen receptor β: Potential target for therapy in adult granulosa cell tumors?
    Gynecol Oncol. 150 (1): 158-65.
  8. Bai, Y. et al. (2018) Oestrogen receptor β5 and epidermal growth factor receptor synergistically promote lung cancer progression.
    Autoimmunity. 51 (4): 157-165.
  9. View The Latest Product References
  10. Wang, M. et al. (2019) The alteration of ERβ5 and collagen metabolism is relevant to the development of stress urinary incontinence
    Int J Clin Exp Med 12(5): 5154-61.
  11. Ciucci, A. et al. (2014) Prognostic significance of the estrogen receptor beta (ERβ) isoforms ERβ1, ERβ2, and ERβ5 in advanced serous ovarian cancer.
    Gynecol Oncol. 132 (2): 351-9.
  12. Younes, M. et al. (2018) Expression of estrogen receptor beta isoforms in pancreatic adenocarcinoma.
    Oncotarget. 9 (102): 37715-20.
  13. Hexiao, T. et al. (2021) Knockdown of CENPF inhibits the progression of lung adenocarcinoma mediated by ERβ2/5 pathway.
    Aging (Albany NY). 13 (2): 2604-25.

Immunofluorescence

RRID
AB_1604962
UniProt
Q92731
Entrez Gene
ESR2
View more products with ESTROGEN RECEPTOR specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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