Estrogen Receptor Alpha antibody | 6F11

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Mouse anti Human Estrogen Receptor Alpha

Product Type
Monoclonal Antibody
Clone
6F11
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1799T C* P* 0.1 ml
MCA1799 C* P* WB 1 ml
Mouse anti Human estrogen alpha antibody, clone 6F11 recognizes the human estrogen receptor alpha chain (ERα), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. ERα is a ~65 kDa steroid hormone receptor containing an N-terminal (AF-1) ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an (AF-2) domain. ERα binds to DNA as a homodimer (Klinge 2001) and can also form heterodimers with Estrogen receptor beta.

The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors (Yaziji et al. 2008). Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patients prognosis and the effectiveness of hormonal therapy (Bhargava et al. 2012).

Mouse anti human estrogen alpha antibody, clone 6F11 has been used successfully for identification of ERα on breast cancer cell lysates using Western blotting and for the immunohistochemical detection of ERα in breast cancer tissues (Ambroise et al. 2011).

Product Details

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant human estrogen receptor (alpha form).
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line.

Storage Information

Storage
Pack Size: 0.1 ml
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 1 ml
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date

More Information

UniProt
P03372 Related reagents
Entrez Gene
ESR1 Related reagents
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005496 steroid binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0008013 beta-catenin binding
GO:0008270 zinc ion binding
GO:0016585 chromatin remodeling complex
GO:0019899 enzyme binding
GO:0030235 nitric-oxide synthase regulator activity
GO:0030284 estrogen receptor activity
GO:0032355 response to estradiol stimulus
GO:0034056 estrogen response element binding
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0048386 positive regulation of retinoic acid receptor signaling pathway
Regulatory
For research purposes only

Applications of Estrogen Receptor Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1 1/40 1/60
Immunohistology - Paraffin 2 1/40 1/80
Western Blotting 1/50 1/100
  1. 1The use of Zamboni's fixative is recommended for best results
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Breast carcinoma

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Estrogen Receptor Alpha antibody

  1. Bevitt, D.J. et al. (1997) New monoclonal antibodies to oestrogen and progesterone receptors effective for paraffin section immunohistochemistry.
    J Pathol. 183 (2): 228-32.
  2. Jongen, V. et al. (2009) Expression of estrogen receptor-alpha and -beta and progesterone receptor-A and -B in a large cohort of patients with endometrioid endometrial cancer.
    Gynecol Oncol. 112: 537-42.
  3. Ambroise, M. et al. (2011) Immunohistochemical profile of breast cancer patients at a tertiary care hospital in South India.
    Asian Pac J Cancer Prev. 12: 625-9.
  4. Droog M et al. (2017) Estrogen receptor &alpha wields treatment-specific enhancers between morphologically similar endometrial tumors.
    Proc Natl Acad Sci U S A. : .
  5. Krishnaswamy, U. et al. (2013) Correlation of Her-2 neu over-expression with clinico pathological features of carcinoma breast
    Apollo Med. 10 (4): 313-7.