ErbB3 antibody | SGP1

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Mouse anti Human ErbB3

Product Type: Monoclonal Antibody
Clone: SGP1
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1410	C* E F IP			0.1 mg

Mouse anti Human ErbB3 antibody, clone SGP1 recognizes human Receptor tyrosine-protein kinase erbB-3, also known as Proto-oncogene-like protein c-ErbB-3 or Tyrosine kinase-type cell surface receptor HER3. ErbB3 is a 1342 amino acid ~180 kDa glycoprotein containing a single protein kinase domain. Five isoforms of Erb-B3 are generated by alternative splicing. Mouse anti Human ErbB3 antibody, clone SGP1 recognizes a conformation dependent epitope on the extracellular protein core of ErbB3 rather than a carbohydrate moiety created during post-translational modification.

ErbB3 is expressed widely in normal human tissues, but is over expressed in some tumours, including those of the breast, stomach, pancreas and colon (Lemoine et al. 1992). Mutations in the ERBB3 gene can lead to development of Lethal congenital contractural syndrome type 2, associated with atrophy of the spinal cord (Narkis et al. 2007).

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Wheat germ lectin purified ErbB3 protein

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.

Storage Information

Storage: Store at +4^oC for one month or at -20^oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P21860 Related reagents
Entrez Gene: ERBB3 Related reagents
GO Terms: GO:0004714 transmembrane receptor protein tyrosine kinase activity; GO:0004716 receptor signaling protein tyrosine kinase activity; GO:0019838 growth factor binding; GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway; GO:0005887 integral to plasma membrane; GO:0005524 ATP binding; GO:0005615 extracellular space; GO:0006468 protein phosphorylation; GO:0007162 negative regulation of cell adhesion; GO:0007507 heart development; GO:0009968 negative regulation of signal transduction; GO:0014037 Schwann cell differentiation; GO:0014065 phosphatidylinositol 3-kinase cascade; GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade; GO:0016323 basolateral plasma membrane; GO:0021545 cranial nerve development; GO:0030296 protein tyrosine kinase activator activity; GO:0042060 wound healing; GO:0042127 regulation of cell proliferation; GO:0042803 protein homodimerization activity; GO:0043235 receptor complex; GO:0043524 negative regulation of neuron apoptosis; GO:0046982 protein heterodimerization activity; GO:0051048 negative regulation of secretion; GO:0051402 neuron apoptosis
Regulatory: For research purposes only

Applications of ErbB3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen ¹
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting

¹The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Flow Cytometry

Product Specific References

References for ErbB3 antibody

Rajkumar, T. & Gullick, W.J. (1994) A monoclonal antibody to the human c-erbB3 protein stimulates the anchorage-independent growth of breast cancer cell lines.
Br J Cancer. 70 (3): 459-65.
Rajkumar, T. et al. (1995) c-erbB3 expression in breast tumour derived cell lines.
The Breast 4: 84-91.
Beerli, R.R. et al. (2000) Positive and negative regulation of endogenous genes by designed transcription factors.
Proc Natl Acad Sci U S A. 97: 1495-500.

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