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Eosinophil Major Basic Protein antibody | BMK-13

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Mouse anti Human eosinophil major basic protein antibody, clone BMK-13 (MCA5751) used for the demonstration of eosinophil MBP expression in duodenal tissue sections of patients with functional dyspepsia using immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
MBP immunostaining of eosinophil degranulation in the representative specimens (D2 and D1) from patients with FD and healthy controls. Magnification, 400×.

From: Du L, Shen J, Kim JJ, Yu Y, Ma L, Dai N.
Increased Duodenal Eosinophil Degranulation in Patients with Functional Dyspepsia: A Prospective Study.
Sci Rep. 2016 Oct 6;6:34305.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human Eosinophil Major Basic Protein

Product Type
Monoclonal Antibody
Clone
BMK-13
Isotype
IgG1
Specificity
Eosinophil Major Basic Protein

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Product Code Applications Pack Size List Price Your Price Qty
MCA5751
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * P * 0.1 mg loader
List Price Your Price
loader

Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 recongises the Eosinophil Major Basic Protein (EMBP), a 117 amino acid protein, corresponding to residues 106-222 of Bone marrow proteoglycan (precursor). Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 stains both resting and activated eosinophils of bronchial and skin sections of allergic and normal sites and may be considered a Pan eosinophil marker. Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 cross reacts weakly with basophils which also contain low levels of EMBP. No cross reactivity with other human cells or proteins has been noted.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Guinea Pig Weak
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1 1/20 1/50
Immunohistology - Paraffin 2 1/20 1/50
  1. 1 It is recommended that sections are fixed in a 1:1 mixture of acetone and methanol and air-dried for 1 hour. Good results may be achieved via staining with the APAAP method.
  2. 2 This product requires enzymatic pre-treatment of paraffin sections prior to staining. Pepsin is recommended for this purpose. NB. Heat-mediated antigen retrieval methods should not be used.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Eosinophil Major Basic Protein antibody

  1. Moqbel, R. et al. (1992) Application of monoclonal antibodies against major basic protein (BMK-13) and eosinophil cationic protein (EG1 and EG2) for quantifying eosinophils in bronchial biopsies from atopic asthma.
    Clin Exp Allergy. 22 (2): 265-73.
  2. Hashimoto, Y. et al. (1993) Purification of the antibacterial fragments of guinea-pig major basic protein.
    Biochim Biophys Acta. 1203 (2): 236-42.
  3. Haczku, A. et al. (1995) T-cells subsets and activation in bronchial mucosa of sensitized Brown-Norway rats after single allergen exposure.
    Immunology. 85 (4): 591-7.
  4. Underwood, S. et al. (1995) Time-course of antigen-induced airway inflammation in the guinea-pig and its relationship to airway hyperresponsiveness.
    Eur Respir J. 8 (12): 2104-13.
  5. Mishima, H. et al. (1998) CD4+ T cells can induce airway hyperresponsiveness to allergen challenge in the brown norway rat.
    Am J Respir Crit Care Med. 158 (6): 1863-70.
  6. Lacy, P. et al. (1998) Intracellular localization of interleukin-6 in eosinophils from atopic asthmatics and effects of interferon gamma.
    Blood. 91 (7): 2508-16.
  7. Lacy, P. et al. (1999) Rapid mobilization of intracellularly stored RANTES in response to interferon-gamma in human eosinophils.
    Blood. 94 (1): 23-32.
  8. Walsh, G.M. et al. (1999) Resting and cytokine-stimulated human small airway epithelial cells recognize and engulf apoptotic eosinophils.
    Blood. 94 (8): 2827-35.
    9. View The Latest Product References
  9. Cameron, L. et al. (2000) Evidence for local eosinophil differentiation within allergic nasal mucosa: inhibition with soluble IL-5 receptor.
    J Immunol. 164 (3): 1538-45.
  10. Mahmudi-azer, S. et al. (2002) Translocation of the tetraspanin CD63 in association with human eosinophil mediator release.
    Blood. 99 (11): 4039-47.
  11. Lacy, P. et al. (2003) Divergence of mechanisms regulating respiratory burst in blood and sputum eosinophils and neutrophils from atopic subjects.
    J Immunol. 170 (5): 2670-9.
  12. Isogai S et al. (2003) The effects of CD8+γδ T cells on late allergic airway responses and airway inflammation in rats.
    J Allergy Clin Immunol. 112 (3): 547-55.
  13. Al-Rabia, M.W. et al. (2004) Membrane receptor-mediated apoptosis and caspase activation in the differentiated EoL-1 eosinophilic cell line.
    J Leukoc Biol. 75 (6): 1045-55.
  14. Tulic, M.K. et al. (2009) Thymic indoleamine 2,3-dioxygenase-positive eosinophils in young children: potential role in maturation of the naive immune system.
    Am J Pathol. 175 (5): 2043-52.
  15. Dellon, E.S. et al. (2012) Diagnostic utility of major basic protein, eotaxin-3, and leukotriene enzyme staining in eosinophilic esophagitis.
    Am J Gastroenterol. 107 (10): 1503-11.
  16. Vanheel, H. et al. (2014) Impaired duodenal mucosal integrity and low-grade inflammation in functional dyspepsia.
    Gut. 63 (2): 262-71.
  17. Cirillo, C. et al. (2015) Evidence for neuronal and structural changes in submucous ganglia of patients with functional dyspepsia.
    Am J Gastroenterol. 110 (8): 1205-15.
  18. Wiersma, L.C. et al. (2015) Pathogenesis of infection with 2009 pandemic H1N1 influenza virus in isogenic guinea pigs after intranasal or intratracheal inoculation.
    Am J Pathol. 185 (3): 643-50.
  19. Wolf, W.A. et al. (2015) Predictors of response to steroid therapy for eosinophilic esophagitis and treatment of steroid-refractory patients.
    Clin Gastroenterol Hepatol. 13 (3): 452-8.
  20. Du, L. et al. (2016) Increased Duodenal Eosinophil Degranulation in Patients with Functional Dyspepsia: A Prospective Study.
    Sci Rep. 6: 34305.
  21. Tyler, M.A. et al. (2017) Large-scale gene expression profiling reveals distinct type 2 inflammatory patterns in chronic rhinosinusitis subtypes.
    J Allergy Clin Immunol. 139 (3): 1061-1064.e4.
  22. Whelan, K.A. et al. (2020) Persistent Basal Cell Hyperplasia Is Associated With Clinical and Endoscopic Findings in Patients With Histologically Inactive Eosinophilic Esophagitis.
    Clin Gastroenterol Hepatol. 18 (7): 1475-1482.e1.
  23. Dellon, E.S. et al. (2020) Utility of major basic protein, eotaxin-3, and mast cell tryptase staining for prediction of response to topical steroid treatment in eosinophilic esophagitis: analysis of a randomized, double-blind, double dummy clinical trial.
    Dis Esophagus. 33(6):doaa003.
  24. Duan, S. et al. (2021) Eosinophil-associated microinflammation in the gastroduodenal tract contributes to gastric hypersensitivity in a rat model of early-life adversity.
    Am J Physiol Gastrointest Liver Physiol. 320 (2): G206-G216.
  25. Duan, S. et al. (2022) Yokukansan Suppresses Gastric Hypersensitivity and Eosinophil-associated Microinflammation in Rats With Functional Dyspepsia.
    J Neurogastroenterol Motil. 28 (2): 255-64.
  26. Chikkamenahalli, L.L. et al. (2024) Single cell atlas of human gastric muscle immune cells and macrophage-driven changes in idiopathic gastroparesis.
    iScience. 27 (3): 108991.

Flow Cytometry

Immunofluorescence

Immunohistology - Paraffin

RRID
AB_10671914
UniProt
P13727
Entrez Gene
PRG2
GO Terms
GO:0042742 defense response to bacterium
GO:0005576 extracellular region
GO:0005529 sugar binding
GO:0030133 transport vesicle
GO:0006955 immune response
GO:0008201 heparin binding
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