Eosinophil Major Basic Protein antibody | BMK-13
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Mouse anti Human Eosinophil Major Basic Protein
- Product Type
- Monoclonal Antibody
- Eosinophil Major Basic Protein
|Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 recongises the Eosinophil Major Basic Protein (EMBP), a 117 amino acid protein, corresponding to residues 106-222 of Bone marrow proteoglycan (precursor). Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 stains both resting and activated eosinophils of bronchial and skin sections of allergic and normal sites and may be considered a Pan eosinophil marker. Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 cross reacts weakly with basophils which also contain low levels of EMBP. No cross reactivity with other human cells or proteins has been noted.|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat Guinea Pig Weak
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- For research purposes only
- Guaranteed until date of expiry. Please see product label.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1||1/20||1/50|
|Immunohistology - Paraffin 2||1/20||1/50|
- 1 It is recommended that sections are fixed in a 1:1 mixture of acetone and methanol and air-dried for 1 hour. Good results may be achieved via staining with the APAAP method.
- 2 This product requires enzymatic pre-treatment of paraffin sections prior to staining. Pepsin is recommended for this purpose. NB. Heat-mediated antigen retrieval methods should not be used.
References for Eosinophil Major Basic Protein antibody
Moqbel, R. et al. (1992) Application of monoclonal antibodies against major basic protein (BMK-13) and eosinophil cationic protein (EG1 and EG2) for quantifying eosinophils in bronchial biopsies from atopic asthma.
Clin Exp Allergy. 22 (2): 265-73.
Hashimoto, Y. et al. (1993) Purification of the antibacterial fragments of guinea-pig major basic protein.
Biochim Biophys Acta. 1203 (2): 236-42.
Haczku, A. et al. (1995) T-cells subsets and activation in bronchial mucosa of sensitized Brown-Norway rats after single allergen exposure.
Immunology. 85 (4): 591-7.
Underwood, S. et al. (1995) Time-course of antigen-induced airway inflammation in the guinea-pig and its relationship to airway hyperresponsiveness.
Eur Respir J. 8 (12): 2104-13.
Mishima, H. et al. (1998) CD4+ T cells can induce airway hyperresponsiveness to allergen challenge in the brown norway rat.
Am J Respir Crit Care Med. 158 (6): 1863-70.
Lacy, P. et al. (1998) Intracellular localization of interleukin-6 in eosinophils from atopic asthmatics and effects of interferon gamma.
Blood. 91 (7): 2508-16.
Lacy, P. et al. (1999) Rapid mobilization of intracellularly stored RANTES in response to interferon-gamma in human eosinophils.
Blood. 94 (1): 23-32.
Walsh, G.M. et al. (1999) Resting and cytokine-stimulated human small airway epithelial cells recognize and engulf apoptotic eosinophils.
Blood. 94 (8): 2827-35.
View The Latest Product References
Cameron, L. et al. (2000) Evidence for local eosinophil differentiation within allergic nasal mucosa: inhibition with soluble IL-5 receptor.
J Immunol. 164 (3): 1538-45.
Mahmudi-azer, S. et al. (2002) Translocation of the tetraspanin CD63 in association with human eosinophil mediator release.
Blood. 99 (11): 4039-47.
Lacy, P. et al. (2003) Divergence of mechanisms regulating respiratory burst in blood and sputum eosinophils and neutrophils from atopic subjects.
J Immunol. 170 (5): 2670-9.
Isogai S et al. (2003) The effects of CD8+γδ T cells on late allergic airway responses and airway inflammation in rats.
J Allergy Clin Immunol. 112 (3): 547-55.
Al-Rabia, M.W. et al. (2004) Membrane receptor-mediated apoptosis and caspase activation in the differentiated EoL-1 eosinophilic cell line.
J Leukoc Biol. 75 (6): 1045-55.
Tulic, M.K. et al. (2009) Thymic indoleamine 2,3-dioxygenase-positive eosinophils in young children: potential role in maturation of the naive immune system.
Am J Pathol. 175 (5): 2043-52.
Dellon, E.S. et al. (2012) Diagnostic utility of major basic protein, eotaxin-3, and leukotriene enzyme staining in eosinophilic esophagitis.
Am J Gastroenterol. 107 (10): 1503-11.
Vanheel, H. et al. (2014) Impaired duodenal mucosal integrity and low-grade inflammation in functional dyspepsia.
Gut. 63 (2): 262-71.
Cirillo, C. et al. (2015) Evidence for neuronal and structural changes in submucous ganglia of patients with functional dyspepsia.
Am J Gastroenterol. 110 (8): 1205-15.
Wiersma, L.C. et al. (2015) Pathogenesis of infection with 2009 pandemic H1N1 influenza virus in isogenic guinea pigs after intranasal or intratracheal inoculation.
Am J Pathol. 185 (3): 643-50.
Wolf, W.A. et al. (2015) Predictors of response to steroid therapy for eosinophilic esophagitis and treatment of steroid-refractory patients.
Clin Gastroenterol Hepatol. 13 (3): 452-8.
Du, L. et al. (2016) Increased Duodenal Eosinophil Degranulation in Patients with Functional Dyspepsia: A Prospective Study.
Sci Rep. 6: 34305.
Tyler, M.A. et al. (2017) Large-scale gene expression profiling reveals distinct type 2 inflammatory patterns in chronic rhinosinusitis subtypes.
J Allergy Clin Immunol. 139 (3): 1061-1064.e4.
Whelan, K.A. et al. (2019) Persistent Basal Cell Hyperplasia is Associated with Clinical and Endoscopic Findings in Patients With Histologically Inactive Eosinophilic Esophagitis.
Clin Gastroenterol Hepatol. Sep 06 [Epub ahead of print].
Dellon, E.S. et al. (2020) Utility of major basic protein, eotaxin-3, and mast cell tryptase staining for prediction of response to topical steroid treatment in eosinophilic esophagitis: analysis of a randomized, double-blind, double dummy clinical trial.
Dis Esophagus. 33(6):doaa003.
Duan, S. et al. (2021) Eosinophil-associated microinflammation in the gastroduodenal tract contributes to gastric hypersensitivity in a rat model of early-life adversity.
Am J Physiol Gastrointest Liver Physiol. 320 (2): G206-G216.
Duan, S. et al. (2022) Yokukansan Suppresses Gastric Hypersensitivity and Eosinophil-associated Microinflammation in Rats With Functional Dyspepsia.
J Neurogastroenterol Motil. 28 (2): 255-64.
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