Eosinophil Major Basic Protein antibody | BMK-13

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Mouse anti Human Eosinophil Major Basic Protein

Product Type
Monoclonal Antibody
Clone
BMK-13
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5751 C* P* 0.1 mg
Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 recongises the Eosinophil Major Basic Protein (EMBP), a 117 amino acid protein, corresponding to residues 106-222 of Bone marrow proteoglycan (precursor). Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 stains both resting and activated eosinophils of bronchial and skin sections of allergic and normal sites and may be considered a Pan eosinophil marker. Mouse anti Human Eosinophil Major Basic Protein antibody, clone BMK-13 cross reacts weakly with basophils which also contain low levels of EMBP. No cross reactivity with other human cells or proteins has been noted.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Guinea Pig Weak
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P13727 Related reagents
Entrez Gene
PRG2 Related reagents
GO Terms
GO:0042742 defense response to bacterium
GO:0005576 extracellular region
GO:0005529 sugar binding
GO:0030133 transport vesicle
GO:0006955 immune response
GO:0008201 heparin binding
Regulatory
For research purposes only

Applications of Eosinophil Major Basic Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1 1/20 1/50
Immunohistology - Paraffin 2 1/20 1/50
  1. 1 It is recommended that sections are fixed in a 1:1 mixture of acetone and methanol and air-dried for 1 hour. Good results may be achieved via staining with the APAAP method.
  2. 2 This product requires enzymatic pre-treatment of paraffin sections prior to staining. Pepsin is recommended for this purpose. NB. Heat-mediated antigen retrieval methods should not be used.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Flow Cytometry

Immunohistology - Paraffin

Product Specific References

References for Eosinophil Major Basic Protein antibody

  1. Moqbel, R. et al. (1992) Application of monoclonal antibodies against major basic protein (BMK-13) and eosinophil cationic protein (EG1 and EG2) for quantifying eosinophils in bronchial biopsies from atopic asthma.
    Clin Exp Allergy. 22 (2): 265-73.
  2. Haczku, A. et al. (1995) T-cells subsets and activation in bronchial mucosa of sensitized Brown-Norway rats after single allergen exposure.
    Immunology. 85 (4): 591-7.
  3. Hashimoto, Y. et al. (1993) Purification of the antibacterial fragments of guinea-pig major basic protein.
    Biochim Biophys Acta. 1203 (2): 236-42.
  4. Underwood, S. et al. (1995) Time-course of antigen-induced airway inflammation in the guinea-pig and its relationship to airway hyperresponsiveness.
    Eur Respir J. 8 (12): 2104-13.
  5. Cameron, L. et al. (2000) Evidence for local eosinophil differentiation within allergic nasal mucosa: inhibition with soluble IL-5 receptor.
    J Immunol. 164 (3): 1538-45.
  6. Walsh, G.M. et al. (1999) Resting and cytokine-stimulated human small airway epithelial cells recognize and engulf apoptotic eosinophils.
    Blood. 94 (8): 2827-35.
  7. Lacy, P. et al. (1999) Rapid mobilization of intracellularly stored RANTES in response to interferon-gamma in human eosinophils.
    Blood. 94 (1): 23-32.
  8. Mishima, H. et al. (1998) CD4+ T cells can induce airway hyperresponsiveness to allergen challenge in the brown norway rat.
    Am J Respir Crit Care Med. 158 (6): 1863-70.
  9. Lacy, P. et al. (2003) Divergence of mechanisms regulating respiratory burst in blood and sputum eosinophils and neutrophils from atopic subjects.
    J Immunol. 170 (5): 2670-9.
  10. Lacy, P. et al. (1998) Intracellular localization of interleukin-6 in eosinophils from atopic asthmatics and effects of interferon gamma.
    Blood. 91 (7): 2508-16.
  11. Tulic, M.K. et al. (2009) Thymic indoleamine 2,3-dioxygenase-positive eosinophils in young children: potential role in maturation of the naive immune system.
    Am J Pathol. 175 (5): 2043-52.
  12. Mahmudi-azer, S. et al. (2002) Translocation of the tetraspanin CD63 in association with human eosinophil mediator release.
    Blood. 99 (11): 4039-47.
  13. Du, L. et al. (2016) Increased Duodenal Eosinophil Degranulation in Patients with Functional Dyspepsia: A Prospective Study.
    Sci Rep. 6: 34305.
  14. Vanheel, H. et al. (2014) Impaired duodenal mucosal integrity and low-grade inflammation in functional dyspepsia.
    Gut. 63 (2): 262-71.
  15. Haczku, A. et al. (1995) T-cells subsets and activation in bronchial mucosa of sensitized Brown-Norway rats after single allergen exposure.
    Immunology. 85 (4): 591-7.
  16. Wiersma, L.C. et al. (2015) Pathogenesis of infection with 2009 pandemic H1N1 influenza virus in isogenic guinea pigs after intranasal or intratracheal inoculation.
    Am J Pathol. 185 (3): 643-50.
  17. Tyler, M.A. et al. (2017) Large-scale gene expression profiling reveals distinct type 2 inflammatory patterns in chronic rhinosinusitis subtypes.
    J Allergy Clin Immunol. 139 (3): 1061-1064.e4.
  18. Wolf, W.A. et al. (2015) Predictors of response to steroid therapy for eosinophilic esophagitis and treatment of steroid-refractory patients.
    Clin Gastroenterol Hepatol. 13 (3): 452-8.
  19. Al-Rabia, M.W. et al. (2004) Membrane receptor-mediated apoptosis and caspase activation in the differentiated EoL-1 eosinophilic cell line.
    J Leukoc Biol. 75 (6): 1045-55.
  20. Dellon, E.S. et al. (2012) Diagnostic utility of major basic protein, eotaxin-3, and leukotriene enzyme staining in eosinophilic esophagitis.
    Am J Gastroenterol. 107 (10): 1503-11.
  21. Isogai S et al. (2003) The effects of CD8+γδ T cells on late allergic airway responses and airway inflammation in rats.
    J Allergy Clin Immunol. 112 (3): 547-55.
  22. Cirillo, C. et al. (2015) Evidence for neuronal and structural changes in submucous ganglia of patients with functional dyspepsia.
    Am J Gastroenterol. 110 (8): 1205-15.