Endothelin 1 antibody | IC4

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Mouse anti Human Endothelin 1

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.1 mg loader

Mouse anti Human endothelin 1 antibody, clone IC4 recognizes human endothelin 1 (ET-1). Endothelin-1 in it's precursor form is a 212 amino acid protein with a 17 aa signal peptide, an N-terminal pro-peptide region and a C-terminal pro-peptide region flanking the endothelin peptides, big endothelin-1 (38aa), the precursor of the smaller endothelin peptide which is cleaved by endothlin converting enzyme to produce the active endothelin-1 (21aa) peptide and a c-terminal fragment. Endothelin-1 is a potent vasoconstrictor with a molecular mass of approximately 25 kDa. Mouse anti human endothelin 1 antibody, clone IC4 shows less than 10% reactivity with endothelin 3.

Plasma levels of both endothlin-1 and big endothlin-1 are useful as predictors for survival of patients with congestive heart failure (Rodeheffer et al. 1992). However, the half life of the endothlin1 peptide in human plasma is very short (c1.0 mins) and measurement of big-endothlin-1 may be a more reliable measure of the endothlin secretory pathway activity (Hemsén et al. 1995).

Product Details

Target Species
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
C-terminal fragment of human endothelin 1.
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0001516 prostaglandin biosynthetic process
GO:0003100 regulation of systemic arterial blood pressure by endothelin
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0019722 calcium-mediated signaling
GO:0010460 positive regulation of heart rate
GO:0010595 positive regulation of endothelial cell migration
GO:0010613 positive regulation of cardiac muscle hypertrophy
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0014824 artery smooth muscle contraction
GO:0014826 vein smooth muscle contraction
GO:0046887 positive regulation of hormone secretion
GO:0019229 regulation of vasoconstriction
GO:0030072 peptide hormone secretion
GO:0030185 nitric oxide transport
GO:0030195 negative regulation of blood coagulation
GO:0031707 endothelin A receptor binding
GO:0031708 endothelin B receptor binding
GO:0042313 protein kinase C deactivation
GO:0043507 positive regulation of JUN kinase activity
GO:0045321 leukocyte activation
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045793 positive regulation of cell size
GO:0045840 positive regulation of mitosis
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0051771 negative regulation of nitric-oxide synthase biosynthetic process
GO:0060298 positive regulation of sarcomere organization
GO:0060585 positive regulation of prostaglandin-endoperoxide synthase activity
For research purposes only

Applications of Endothelin 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Endothelin 1 antibody

  1. Papalambros, E. et al. (2003) Immunohistochemical expression of metalloproteinases MMP-2 and MMP-9 in abdominal aortic aneurysms: correlation with symptoms and aortic diameter.
    Int J Mol Med. 12 (6): 965-8.

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