Desmoplakin 1/2 antibody | DP-2.15
Mouse anti Human desmoplakin 1/2 antibody, clone DP-2.15 used for the detection of desmoplakin expressing human cardiomyocytes by immunofluorescence.
Image caption:
Cardiac matrix coatings increase hESC derived cardiomyocyte maturation compared to the standard gelatin substrate. hESC-derived cardiomyocytes were cultured on gelatin (a and c) or cardiac matrix (b and d) and stained for Titin M8 (red), desmoplakin (green), or nuclei (blue). Cell cluster area (e), number of nuclei per cluster (f), and average desmoplakin positive area (g) were measured and compared between the two coatings. Cardiomyocytes had an increased cluster area and number of nuclei at both day 35 and 112. At day 112, the average desmoplakin immunoreactive area was significantly greater on the cardiac matrix, with localization observed at the lateral ends of the cardiomyocytes (arrows), indicating the formation of desmosomes and a more mature phenotype. **p<0.01.
From: DeQuach JA, Mezzano V, Miglani A, Lange S, Keller GM, et al. (2010) Simple and High Yielding Method for Preparing Tissue Specific Extracellular Matrix Coatings for Cell Culture.
PLoS ONE 5(9): e13039.
Mouse anti Desmoplakin 1/2 antibody used for the detection of desmoplakin expressing cells in porcine meninges by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of the porcine meninges. The meninges composed of the dura mater (A–G), the arachnoid mater (H–I) and the pia mater (J–M) in formalin fixed tissue. The porcine spinal cord was cryosectioned and the porcine dura mater (N–O) was stained. The tissue sections were labeled for: (A) von Willebrand factor, (B and K) fibronectin, (C and J) vimentin, (D) laminin, (E) collagen I, (F and M) collagen II, (G, H, and L) integrin 1β, (I) Desmoplakin, (N) collagen III, (O) E-cadherin. Images were captured at ×200 magnification.
From: Papageorgiou I, Marsh R, Tipper JL, Hall RM, Fisher J, Ingham E. Interaction of micron and nano-sized particles with cells of the dura mater.
J Biomed Mater Res B Appl Biomater. 2014 Oct;102(7):1496-505.
Mouse anti Human desmoplakin 1/2 antibody, clone DP-2.15 (2722-5204) used to demonstrate desmoplakin expression in BeWo cells in vitro by immunofluorescence.
Image caption:
Validation of trophoblastic properties in newly derived BeWo-NG cells.
c) BeWo-NG cells can undergo syncytilialization after FSK A stimulation. Confluent cells were treated with 10 μmol/L FSK A for 24 h. Cells were fixed in cold acetone/methanol and permeabilization using 0.1% saponin in 1% BSA. Cells were then stained with desmoplakin specific primary antibody and Alexa 488 conjugated secondary antibody before visualizationusing fluorescent microscopy. The nuclei of cells were counterstained with DAPI nuclear dye. Red lines are drawn around the boundary of Syncytialized cells. Micrographs were taken under 200X magnification
From: Yung HW, Alnæs-Katjavivi P, Jones CJ, El-Bacha T, Golic M, Staff AC, Burton GJ.
Placental endoplasmic reticulum stress in gestational diabetes: the potential for therapeutic intervention with chemical chaperones and antioxidants.
Diabetologia. 2016 Oct;59(10):2240-50.
Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 recognizes both desmoplakin 1 and 2 from stratified epithelia, simple epithelia including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges. Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 can be used for the detection of primary and metastatic carcinomas.
Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 recognizes the N terminus of the rod domain of desmoplakin ( Bornslaeger et al. 1996).
Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 recognizes the N terminus of the rod domain of desmoplakin ( Bornslaeger et al. 1996).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Chicken Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Native bovine desmoplakin 1 and 2 purified from bovine snout desmosomes.
- Approx. Protein Concentrations
- IgG concentration 1mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells from the NS0 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P15924 Related reagents
- Entrez Gene
- DSP Related reagents
- GO Terms
- GO:0001533 cornified envelope
- GO:0030674 protein binding, bridging
- GO:0005200 structural constituent of cytoskeleton
- GO:0006921 cellular component disassembly involved in apoptosis
- GO:0018149 peptide cross-linking
- GO:0030057 desmosome
- GO:0030216 keratinocyte differentiation
- Regulatory
- For research purposes only
Applications of Desmoplakin 1/2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Desmoplakin 1/2 antibody
-
Cowin, P. et al. (1985) The complement of desmosomal plaque proteins in different cell types.
J Cell Biol. 101 (4): 1442-54. -
Jheon, A.H. et al. (2011) PERP regulates enamel formation via effects on cell-cell adhesion and gene expression.
J Cell Sci. 124: 745-54. -
Bornslaeger, E.A. et al. (1996) Breaking the connection: displacement of the desmosomal plaque protein desmoplakin from cell-cell interfaces disrupts anchorage of intermediate filament bundles and alters intercellular junction assembly.
J. Cell. Biol. 134: 985-1001. -
Park, D.S. et al. (2015) Genetically engineered SCN5A mutant pig hearts exhibit conduction defects and arrhythmias.
J Clin Invest. 125 (1): 403-12. -
DeQuach JA et al. (2010) Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture.
PLoS One. 5 (9): e13039. -
Basheer, W.A. et al. (2015) Cardiomyocyte-specific overexpression of the ubiquitin ligase Wwp1 contributes to reduction in Connexin 43 and arrhythmogenesis.
J Mol Cell Cardiol. 88: 1-13. -
Yung, H.W. et al. (2016) Placental endoplasmic reticulum stress in gestational diabetes: the potential for therapeutic intervention with chemical chaperones and antioxidants.
Diabetologia. 59 (10): 2240-50. -
Chkourko, H.S. et al. (2012) Remodeling of mechanical junctions and of microtubule-associated proteins accompany cardiac connexin43 lateralization.
Heart Rhythm. 9 (7): 1133-1140.e6. -
Vila, J. et al. (2017) Structural and molecular pathology of the atrium in boxer arrhythmogenic right ventricular cardiomyopathy.
J Vet Cardiol. 19 (1): 57-67. -
Yung, H.W. et al. (2016) Placental endoplasmic reticulum stress in gestational diabetes: the potential for therapeutic intervention with chemical chaperones and antioxidants.
Diabetologia. 59 (10): 2240-50.
