Desmoplakin 1/2 antibody | DP-2.15

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Mouse anti Desmoplakin 1/2

Product Type
Monoclonal Antibody
Clone
DP-2.15
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2722-5204 C IF WB 50 µg
Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 recognizes both desmoplakin 1 and 2 from stratified epithelia, simple epithelia including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges. Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 can be used for the detection of primary and metastatic carcinomas.
Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 recognizes the N terminus of the rod domain of desmoplakin ( Bornslaeger et al. 1996).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Chicken
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Native bovine desmoplakin 1 and 2 purified from bovine snout desmosomes.
Approx. Protein Concentrations
IgG concentration 1mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells from the NS0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P15924 Related reagents
Entrez Gene
DSP Related reagents
GO Terms
GO:0001533 cornified envelope
GO:0030674 protein binding, bridging
GO:0005200 structural constituent of cytoskeleton
GO:0006921 cellular component disassembly involved in apoptosis
GO:0018149 peptide cross-linking
GO:0030057 desmosome
GO:0030216 keratinocyte differentiation
Regulatory
For research purposes only

Applications of Desmoplakin 1/2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunofluorescence

Product Specific References

References for Desmoplakin 1/2 antibody

  1. Cowin, P. et al. (1985) The complement of desmosomal plaque proteins in different cell types.
    J Cell Biol. 101 (4): 1442-54.
  2. Jheon, A.H. et al. (2011) PERP regulates enamel formation via effects on cell-cell adhesion and gene expression.
    J Cell Sci. 124: 745-54.
  3. Bornslaeger, E.A. et al. (1996) Breaking the connection: displacement of the desmosomal plaque protein desmoplakin from cell-cell interfaces disrupts anchorage of intermediate filament bundles and alters intercellular junction assembly.
    J. Cell. Biol. 134: 985-1001.
  4. Park, D.S. et al. (2015) Genetically engineered SCN5A mutant pig hearts exhibit conduction defects and arrhythmias.
    J Clin Invest. 125 (1): 403-12.
  5. DeQuach JA et al. (2010) Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture.
    PLoS One. 5 (9): e13039.
  6. Basheer, W.A. et al. (2015) Cardiomyocyte-specific overexpression of the ubiquitin ligase Wwp1 contributes to reduction in Connexin 43 and arrhythmogenesis.
    J Mol Cell Cardiol. 88: 1-13.
  7. Yung, H.W. et al. (2016) Placental endoplasmic reticulum stress in gestational diabetes: the potential for therapeutic intervention with chemical chaperones and antioxidants.
    Diabetologia. 59 (10): 2240-50.
  8. Chkourko, H.S. et al. (2012) Remodeling of mechanical junctions and of microtubule-associated proteins accompany cardiac connexin43 lateralization.
    Heart Rhythm. 9 (7): 1133-1140.e6.
  9. Vila, J. et al. (2017) Structural and molecular pathology of the atrium in boxer arrhythmogenic right ventricular cardiomyopathy.
    J Vet Cardiol. 19 (1): 57-67.
  10. Yung, H.W. et al. (2016) Placental endoplasmic reticulum stress in gestational diabetes: the potential for therapeutic intervention with chemical chaperones and antioxidants.
    Diabetologia. 59 (10): 2240-50.