Desmoplakin 1/2 antibody | DP-2.15



Mouse anti Desmoplakin 1/2 antibody, clone DP-2.15 recognizes the N terminus of the rod domain of desmoplakin ( Bornslaeger et al. 1996).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Chicken Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Native bovine desmoplakin 1 and 2 purified from bovine snout desmosomes.
- Approx. Protein Concentrations
- IgG concentration 1mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells from the NS0 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P15924
- Entrez Gene
- DSP
- GO Terms
- GO:0001533 cornified envelope
- GO:0030674 protein binding, bridging
- GO:0005200 structural constituent of cytoskeleton
- GO:0006921 cellular component disassembly involved in apoptosis
- GO:0018149 peptide cross-linking
- GO:0030057 desmosome
- GO:0030216 keratinocyte differentiation
- Regulatory
- For research purposes only
Applications of Desmoplakin 1/2 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunofluorescence | |||
Immunohistology - Frozen | |||
Western Blotting |
Secondary Antibodies Available
Product Specific References
References for Desmoplakin 1/2 antibody
-
Cowin, P. et al. (1985) The complement of desmosomal plaque proteins in different cell types.
J Cell Biol. 101 (4): 1442-54. -
Jheon, A.H. et al. (2011) PERP regulates enamel formation via effects on cell-cell adhesion and gene expression.
J Cell Sci. 124: 745-54. -
Bornslaeger, E.A. et al. (1996) Breaking the connection: displacement of the desmosomal plaque protein desmoplakin from cell-cell interfaces disrupts anchorage of intermediate filament bundles and alters intercellular junction assembly.
J. Cell. Biol. 134: 985-1001. -
Park, D.S. et al. (2015) Genetically engineered SCN5A mutant pig hearts exhibit conduction defects and arrhythmias.
J Clin Invest. 125 (1): 403-12. -
DeQuach JA et al. (2010) Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture.
PLoS One. 5 (9): e13039. -
Basheer, W.A. et al. (2015) Cardiomyocyte-specific overexpression of the ubiquitin ligase Wwp1 contributes to reduction in Connexin 43 and arrhythmogenesis.
J Mol Cell Cardiol. 88: 1-13. -
Yung, H.W. et al. (2016) Placental endoplasmic reticulum stress in gestational diabetes: the potential for therapeutic intervention with chemical chaperones and antioxidants.
Diabetologia. 59 (10): 2240-50. -
Chkourko, H.S. et al. (2012) Remodeling of mechanical junctions and of microtubule-associated proteins accompany cardiac connexin43 lateralization.
Heart Rhythm. 9 (7): 1133-1140.e6. -
Vila, J. et al. (2017) Structural and molecular pathology of the atrium in boxer arrhythmogenic right ventricular cardiomyopathy.
J Vet Cardiol. 19 (1): 57-67. -
Yung, H.W. et al. (2016) Placental endoplasmic reticulum stress in gestational diabetes: the potential for therapeutic intervention with chemical chaperones and antioxidants.
Diabetologia. 59 (10): 2240-50.
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