Desmoglein 3 antibody | 5G11
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|Mouse anti Human Desmoglein 3 antibody, clone 5G11 recognizes human desmoglein-3, also known as 130 kDa pemphigus vulgaris antigen or Cadherin family member 6. Desmoglein-3 is a 999 amino acid ~125 kDa single pass type I transmembrane glycoprotein, a core component of the desmosome cell-cell junction between epithelial cells.
Desmoglein-3 is synthesized in the basal and lower suprabasal layers of the skin.
Mouse anti Human Desmoglein 3 antibody, clone 5G11 does not recognise desmoglein-1 or desmoglein-2.
- Target Species
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Recombinant human desmoglein 3.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1|
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. pepsin (See Wu et al). Alternatively it is reported that staining of FFPE material may be enhanced using protein digestion with e.g. pronase, followed by HIER in citrate buffer.
- Histology Positive Control Tissue
References for Desmoglein 3 antibody
Wahl, J.K. 3rd (2002) Generation of monoclonal antibodies specific for desmoglein family members.
Hybrid Hybridomics. 21 (1): 37-44.
Wu, H/ et al. (2000) Protection against pemphigus foliaceus by desmoglein 3 in neonates.
N Engl J Med. 343 (1): 31-5.
Wan, H. et al. (2007) Increased keratinocyte proliferation initiated through downregulation of desmoplakin by RNA interference.
Exp Cell Res. 313: 2336-44.
Szegedi, A. et al. (2008) Protein kinase C isoenzymes differentially regulate the differentiation-dependent expression of adhesion molecules in human epidermal keratinocytes.
Exp Dermatol. 18: 122-9.
O'Shea, C. et al. (2014) Desmosomal defects in acantholytic squamous cell carcinomas.
J Cutan Pathol. 41 (11): 873-9.
Völlner, F. et al. (2016) Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes.
Sci Rep. 6: 28820.
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