Desmoglein 3 antibody | 5G11

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Mouse anti Human Desmoglein 3

Product Type
Monoclonal Antibody
Clone
5G11
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2273T C IF IP P* WB 25 µg
MCA2273 C IF IP P* WB 0.2 mg
Mouse anti Human Desmoglein 3 antibody, clone 5G11 recognizes human desmoglein-3, also known as 130 kDa pemphigus vulgaris antigen or Cadherin family member 6. Desmoglein-3 is a 999 amino acid ~125 kDa single pass type I transmembrane glycoprotein, a core component of the desmosome cell-cell junction between epithelial cells.

Desmoglein-3 is synthesized in the basal and lower suprabasal layers of the skin.

Mouse anti Human Desmoglein 3 antibody, clone 5G11 does not recognise desmoglein-1 or desmoglein-2.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant human desmoglein 3.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P32926 Related reagents
Entrez Gene
DSG3 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0005829 cytosol
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007156 homophilic cell adhesion
GO:0030057 desmosome
Regulatory
For research purposes only

Applications of Desmoglein 3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting 1/100 1/1000
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. pepsin (See Wu et al). Alternatively it is reported that staining of FFPE material may be enhanced using protein digestion with e.g. pronase, followed by HIER in citrate buffer.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Skin.

Product Specific References

References for Desmoglein 3 antibody

  1. Wahl, J.K. 3rd (2002) Generation of monoclonal antibodies specific for desmoglein family members.
    Hybrid Hybridomics. 21 (1): 37-44.
  2. Wu, H/ et al. (2000) Protection against pemphigus foliaceus by desmoglein 3 in neonates.
    N Engl J Med. 343 (1): 31-5.
  3. Wan, H. et al. (2007) Increased keratinocyte proliferation initiated through downregulation of desmoplakin by RNA interference.
    Exp Cell Res. 313: 2336-44.
  4. Szegedi, A. et al. (2008) Protein kinase C isoenzymes differentially regulate the differentiation-dependent expression of adhesion molecules in human epidermal keratinocytes.
    Exp Dermatol. 18: 122-9.
  5. O'Shea, C. et al. (2014) Desmosomal defects in acantholytic squamous cell carcinomas.
    J Cutan Pathol. 41 (11): 873-9.
  6. Völlner, F. et al. (2016) Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes.
    Sci Rep. 6: 28820.

Further Reading

  1. Koch, P.J. et al. (1998) Desmoglein 3 anchors telogen hair in the follicle.
    J Cell Sci. 111 ( Pt 17): 2529-37.
  2. Jolly, P.S. et al. (2010) p38MAPK signaling and desmoglein-3 internalization are linked events in pemphigus acantholysis.
    J Biol Chem. 285: 8936-41.