Desmoglein 2 antibody | 6D8

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Mouse anti Human Desmoglein 2

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.2 mg loader

25 µg loader

Mouse anti Human desmoglein 2 monoclonal antibody, clone 6D8 recognizes human desmoglein 2, a single pass type 1 membrane glycoprotein with 4 extracellular cadherin domains and six cytoplasmic desmoglein repeat sequences, with a predicted molecular weight of ~122 kDa and an apparent molecular weight of ~160-165 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between epithelial cells.

Mouse anti human desmoglein 2, clone 6D8 has been used for the study of the interaction between desmoglein 2 and adenoviruses which exploit desmoglein 2 as a receptor for infection (Wang et al. 2013).

Mouse anti Human desmoglein 2 monoclonal antibody, clone 6D8 recognizes a region encompassing a portion of extracellular domains 3 and 4 (Kolegraff et al. 2011) and does not recognize desmoglein-1 or desmoglein-3 (Wahl et al. 2002).

Product Details

Target Species
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
A-431 cell membranes.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007156 homophilic cell adhesion
GO:0030057 desmosome
For research purposes only

Applications of Desmoglein 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 1/100 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Western Blotting
MCA2272 detects a band of approximately 165kDa in A-431 cell lysates.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images



Western Blotting

Product Specific References

References for Desmoglein 2 antibody

  1. Wahl, J.K. 3rd (2002) Generation of monoclonal antibodies specific for desmoglein family members.
    Hybrid Hybridomics. 21 (1): 37-44.
  2. Hemmoranta, H. et al. (2006) Transcriptional profiling reflects shared and unique characters for CD34+ and CD133+ cells.
    Stem Cells Dev. 15: 839-51.
  3. Wang, H. et al. (2012) A new human DSG2-transgenic mouse model for studying the tropism and pathology of human adenoviruses.
    J Virol. 86 (11): 6286-302.
  4. Gornowicz-Porowska, J. et al. (2011) Loss of correlation between intensities of desmoglein 2 and desmoglein 3 expression in basal cell carcinomas.
    Acta Dermatovenerol Croat. 19: 150-5.
  5. Yamamoto, Y. et al. (2007) Anti-desmoglein 3 (Dsg3) monoclonal antibodies deplete desmosomes of Dsg3 and differ in their Dsg3-depleting activities related to pathogenicity.
    J Biol Chem. 282: 17866-76.
  6. Pietkiewicz, P. et al. (2014) Discordant expression of desmoglein 2 and 3 at the mRNA and protein levels in nodular and superficial basal cell carcinoma revealed by immunohistochemistry and fluorescent in situ hybridization.
    Clin Exp Dermatol. 39 (5): 628-35.
  7. Nava, P. et al. (2007) Desmoglein-2: a novel regulator of apoptosis in the intestinal epithelium.
    Mol Biol Cell. 18: 4565-78.
  8. Wang, H. et al. (2015) Intracellular Signaling and Desmoglein 2 Shedding Triggered by Human Adenoviruses Ad3, Ad14, and Ad14P1.
    J Virol. 89 (21): 10841-59.
  9. Sobolik-Delmaire, T. et al. (2006) Carboxyl terminus of Plakophilin-1 recruits it to plasma membrane, whereas amino terminus recruits desmoplakin and promotes desmosome assembly.
    J Biol Chem. 281 (25): 16962-70.
  10. Keim, S.A. et al. (2008) Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2.
    Hybridoma (Larchmt). 27 (4): 249-58.
  11. Brennan, D. et al. (2012) A role for caveolin-1 in desmoglein binding and desmosome dynamics.
    Oncogene. 31 (13): 1636-48.
  12. Wang, H. et al. (2013) Structural and functional studies on the interaction of adenovirus fiber knobs and desmoglein 2.
    J Virol. 87 (21): 11346-62.
  13. Todorović, V. et al. (2010) Detection of differentially expressed basal cell proteins by mass spectrometry.
    Mol Cell Proteomics. 9 (2): 351-61.
  14. Ishii, K. et al. (2001) Assembly of desmosomal cadherins into desmosomes is isoform dependent.
    J Invest Dermatol. 117 (1): 26-35.
  15. Yumul, R. et al. (2016) Epithelial Junction Opener Improves Oncolytic Adenovirus Therapy in Mouse Tumor Models.
    Hum Gene Ther. 27 (4): 325-37.
  16. Kim, J. et al. (2020) Desmoglein-2 as a prognostic and biomarker in ovarian cancer.
    Cancer Biol Ther. : 1-9.

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