Desmoglein 2 antibody | 6D8
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Mouse anti Human Desmoglein 2
- Product Type
- Monoclonal Antibody
- Clone
- 6D8
- Isotype
- IgG1
- Specificity
- Desmoglein 2
Antibody Quality and Performance Guarantee
| Mouse anti Human desmoglein 2 monoclonal antibody, clone 6D8 recognizes human desmoglein 2, a single pass type 1 membrane glycoprotein with 4 extracellular cadherin domains and six cytoplasmic desmoglein repeat sequences, with a predicted molecular weight of ~122 kDa and an apparent molecular weight of ~160-165 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between epithelial cells. Mouse anti human desmoglein 2, clone 6D8 has been used for the study of the interaction between desmoglein 2 and adenoviruses which exploit desmoglein 2 as a receptor for infection (Wang et al. 2013). Mouse anti Human desmoglein 2 monoclonal antibody, clone 6D8 recognizes a region encompassing a portion of extracellular domains 3 and 4 (Kolegraff et al. 2011) and does not recognize desmoglein-1 or desmoglein-3 (Wahl et al. 2002). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Immunogen
- A-431 cell membranes.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
||
| Immunoprecipitation | |
||
| Western Blotting | |
1/100 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human skin
- Western Blotting
- Mouse anti Human desmoglein 2 antibody, clone 6D8 detects a band of approximately 165 kDa in A-431 cell lysates.
References for Desmoglein 2 antibody
-
Ishii, K. et al. (2001) Assembly of desmosomal cadherins into desmosomes is isoform dependent.
J Invest Dermatol. 117 (1): 26-35. -
Wahl, J.K. 3rd (2002) Generation of monoclonal antibodies specific for desmoglein family members.
Hybrid Hybridomics. 21 (1): 37-44. -
Sobolik-Delmaire, T. et al. (2006) Carboxyl terminus of Plakophilin-1 recruits it to plasma membrane, whereas amino terminus recruits desmoplakin and promotes desmosome assembly.
J Biol Chem. 281 (25): 16962-70. -
Hemmoranta, H. et al. (2006) Transcriptional profiling reflects shared and unique characters for CD34+ and CD133+ cells.
Stem Cells Dev. 15: 839-51. -
Nava, P. et al. (2007) Desmoglein-2: a novel regulator of apoptosis in the intestinal epithelium.
Mol Biol Cell. 18: 4565-78. -
Yamamoto, Y. et al. (2007) Anti-desmoglein 3 (Dsg3) monoclonal antibodies deplete desmosomes of Dsg3 and differ in their Dsg3-depleting activities related to pathogenicity.
J Biol Chem. 282: 17866-76. -
Keim, S.A. et al. (2008) Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2.
Hybridoma (Larchmt). 27 (4): 249-58. -
Todorović, V. et al. (2010) Detection of differentially expressed basal cell proteins by mass spectrometry.
Mol Cell Proteomics. 9 (2): 351-61.
View The Latest Product References
-
Gornowicz-Porowska, J. et al. (2011) Loss of correlation between intensities of desmoglein 2 and desmoglein 3 expression in basal cell carcinomas.
Acta Dermatovenerol Croat. 19: 150-5. -
Brennan, D. et al. (2012) A role for caveolin-1 in desmoglein binding and desmosome dynamics.
Oncogene. 31 (13): 1636-48. -
Wang, H. et al. (2012) A new human DSG2-transgenic mouse model for studying the tropism and pathology of human adenoviruses.
J Virol. 86 (11): 6286-302. -
Wang, H. et al. (2013) Structural and functional studies on the interaction of adenovirus fiber knobs and desmoglein 2.
J Virol. 87 (21): 11346-62. -
Pietkiewicz, P. et al. (2014) Discordant expression of desmoglein 2 and 3 at the mRNA and protein levels in nodular and superficial basal cell carcinoma revealed by immunohistochemistry and fluorescent in situ hybridization.
Clin Exp Dermatol. 39 (5): 628-35. -
Wang, H. et al. (2015) Intracellular Signaling and Desmoglein 2 Shedding Triggered by Human Adenoviruses Ad3, Ad14, and Ad14P1.
J Virol. 89 (21): 10841-59. -
Yumul, R. et al. (2016) Epithelial Junction Opener Improves Oncolytic Adenovirus Therapy in Mouse Tumor Models.
Hum Gene Ther. 27 (4): 325-37. -
Kim, J. et al. (2020) Desmoglein-2 as a prognostic and biomarker in ovarian cancer.
Cancer Biol Ther. : 1-9.
- RRID
- AB_2093429
- UniProt
- Q14126
- Entrez Gene
- DSG2
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0006921 cellular component disassembly involved in apoptosis
- GO:0007156 homophilic cell adhesion
- GO:0030057 desmosome
MCA2272T
166667
200514
MCA2272
154440
165876
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