Dectin-1 antibody | GE2

Mouse anti Human Dectin-1

Product Type
Monoclonal Antibody
Clone
GE2
Isotype
IgG1
Specificity
Dectin-1

Product Code Applications Pack Size List Price Your Price Qty
MCA4661GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
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Mouse anti Human Dectin-1 antibody, clone GE2 recognizes human Dectin-1, also known as beta-glucan receptor (βGR), a C-type lectin domain family member. It is a major pathogen pattern-recognition receptor that binds beta-glucans, glucose polymers that form cell-wall components or exopolymers of yeasts, fungi and some bacteria. Beta-glucans have also been used experimentally and therapeutically as immunomodulators that enhance resistance to bacterial, yeast, viral and protozoan infections, as well as tumor formation. Dectin-1 also acts as a co-stimulatory molecule on T-cells to induce their proliferation and is necessary for the TLR2-mediated inflammatory response. It is highly expressed on peripheral blood leukocytes and dendritic cells.

Human Dectin-1 has two major (βGR-A and βGR-B) and several minor isoforms. βGR-A and βGR-B differ by the presence and absence, respectively, of a stalk region. Mouse anti Human Dectin-1 antibody, clone GE2 recognizes both major isoforms. Clone GE2 could be used to inhibit receptor function.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
NIH3T3 cells expressing full length Beta-glucan receptor.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised C57BL/6 mice were fused with cells of the murine SP2 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for Dectin-1 antibody

  1. Willment, J.A. et al. (2005) The human beta-glucan receptor is widely expressed and functionally equivalent to murine Dectin-1 on primary cells.
    Eur J Immunol. 35 (5): 1539-47.
  2. Harnack, U. et al. (2011) Yeast-derived beta-(1-3),(1-6)-D-glucan induces up-regulation of CD86 on dectin-1-positive human B-lymphoma cell lines.
    Anticancer Res. 31 (12): 4195-9.
  3. Loures, F.V. et al. (2015) Recognition of Aspergillus fumigatus hyphae by human plasmacytoid dendritic cells is mediated by dectin-2 and results in formation of extracellular traps.
    PLoS Pathog. 11 (2): e1004643.
  4. Kyrmizi, I. et al. (2013) Corticosteroids block autophagy protein recruitment in Aspergillus fumigatus phagosomes via targeting dectin-1/Syk kinase signaling.
    J Immunol. 191 (3): 1287-99.
  5. Ali, M.F. et al. (2017) Canonical Stimulation of the NLRP3 Inflammasome by Fungal Antigens Links Innate and Adaptive B-Lymphocyte Responses by Modulating IL-1β and IgM Production.
    Front Immunol. 8: 1504.

Synonyms
Beta Glucan Receptor
RRID
AB_1658098
UniProt
Q9BXN2
Entrez Gene
CLEC7A
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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