Dectin-1 antibody | GE2
Mouse anti Human Dectin-1 antibody, clone GE2 used in functional assays to assess bllocking activity for binding of A. fumigatus hyphae to human plasmacytoid dendritic cells.
Image caption:
A. fumigatus hyphae are recognized by the mannose receptor, Dectin-2, on pDCs. Human pDCs were purified from PBMCs fractions using CD304-coated magnetic beads. (A) pDCs were treated with no inhibitors, dextran (1 mg/mL), mannan (1 mg/mL), laminarin (0.5 mg/mL) or mannan and laminarin for 30 minutes. The pDCs (5×104) were then incubated for 2 hr with A. fumigatus hyphae (5×104). The cell association was quantified by counting number of pDCs associated (touching or spreading over) with hyphae in 10 different fields. The percent cell association was calculated by dividing the number of pDC associated with hyphae by the total number of pDC counted and then multiplying by 100. (B) Same as in A except pDCs were treated with anti-Dectin-1 (100 μg/mL) and anti-Dectin-2 (100 μg/mL) antibodies for 30 minutes prior to incubation with hyphae. Data represent means ± SE of % cell association from three donors. *P<0.05. (C) Representative photomicrographs of pDCs incubated with A. fumigatus hyphae under the conditions described in panel B.
From: Loures FV, Röhm M, Lee CK, Santos E, Wang JP, et al. (2015) Recognition of Aspergillus fumigatus Hyphae by Human Plasmacytoid Dendritic Cells Is Mediated by Dectin-2 and Results in Formation of Extracellular Traps. PLoS Pathog 11(2): e1004643.
Mouse anti Human dectin-1 antibody, clone GE2(MCA4661EL) used to inhibit dectin-1 action on B lymphocytes in vitro.
Image caption:
Dectin-1 partially mediates IL-1β secretion and requires SYK and the transcriptions factors AP-1 and NF-κB. IL-1β ELISA measured in the cell supernatant of curdlan (Curd) stimulated cells in the presence or absence of 1 mg/ml Laminarin (Lamin) (A), 5 μg/ml anti-dectin-1 antibody (α-Dect1) or 5 μg/ml isotype control (Ms IgG) (B), 40 μM of the SYK inhibitor, piceatannol (pice) and 5 μM of the SYK inhibitor, R406 (C), and 10 μM of the NF-κB inhibitor, Bay11, and 10 μM of the AP-1 inhibitor, SR11 (D). Data are representative of at least three donors in each independent experiments (n = 3). *p <0.05, ***p <0.0001.
From: Ali MF, Dasari H, Van Keulen VP, Carmona EM.
Canonical Stimulation of the NLRP3 Inflammasome by Fungal Antigens Links Innate and Adaptive B-Lymphocyte Responses by Modulating IL-1β and IgM Production.
Front Immunol. 2017 Nov 9;8:1504.
doi: 10.3389/fimmu.2017.01504.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Phycoerythrin conjugated Mouse anti Human Dectin-1 antibody, clone GE2 (MCA4661PE) used to evaluate dectin-1 expression on macrophages and neutrophils by flow cytometry.
Image caption:
Histogram of a representative experiment for Dectin-1 receptor blockage for monocytes (a) and neutrophils (b), showing MFI of monocytes or neutrophil cultures (blue line) or monocytes or neutrophils treated with 3.0 μg/mL (orange line) of anti-Dectin-1 monoclonal antibody. The red line represents MFI of the isotypic control.
From: de Quaglia E Silva JC, Della Coletta AM, Gardizani TP, Romagnoli GG, Kaneno R, Dias-Melicio LA.
Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis.
J Immunol Res. 2019 Feb 6;2019:1529189.
doi: 10.1155/2019/1529189.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Dectin-1
- Product Type
- Monoclonal Antibody
- Clone
- GE2
- Isotype
- IgG1
- Specificity
- Dectin-1
Antibody Quality and Performance Guarantee
| Mouse anti Human Dectin-1 antibody, clone GE2 recognizes human Dectin-1, also known as beta-glucan receptor (βGR) , a C-type lectin domain family member. It is a major pathogen pattern-recognition receptor that binds beta-glucans, glucose polymers that form cell-wall components or exopolymers of yeasts, fungi and some bacteria. Beta-glucans have also been used experimentally and therapeutically as immunomodulators that enhance resistance to bacterial, yeast, viral and protozoan infections, as well as tumor formation. Dectin-1 also acts as a co-stimulatory molecule on T-cells to induce their proliferation and is necessary for the TLR2-mediated inflammatory response. It is highly expressed on peripheral blood leukocytes and dendritic cells. Human Dectin-1 has two major (βGR-A and βGR-B) and several minor isoforms. βGR-A and βGR-B differ by the presence and absence, respectively, of a stalk region. Mouse anti Human Dectin-1 antibody, clone GE2 recognizes both major isoforms. Clone GE2 could be used to inhibit receptor function. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- NIH3T3 cells expressing full length Beta-glucan receptor.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized C57BL/6 mice were fused with cells of the murine SP2 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Willment, J.A. et al. (2005) The human beta-glucan receptor is widely expressed and functionally equivalent to murine Dectin-1 on primary cells.
Eur J Immunol. 35 (5): 1539-47.
References for Dectin-1 antibody
-
Harnack, U. et al. (2011) Yeast-derived beta-(1-3),(1-6)-D-glucan induces up-regulation of CD86 on dectin-1-positive human B-lymphoma cell lines.
Anticancer Res. 31 (12): 4195-9. -
Kyrmizi, I. et al. (2013) Corticosteroids block autophagy protein recruitment in Aspergillus fumigatus phagosomes via targeting dectin-1/Syk kinase signaling.
J Immunol. 191 (3): 1287-99. -
Loures, F.V. et al. (2015) Recognition of Aspergillus fumigatus hyphae by human plasmacytoid dendritic cells is mediated by dectin-2 and results in formation of extracellular traps.
PLoS Pathog. 11 (2): e1004643. -
Ali, M.F. et al. (2017) Canonical Stimulation of the NLRP3 Inflammasome by Fungal Antigens Links Innate and Adaptive B-Lymphocyte Responses by Modulating IL-1β and IgM Production.
Front Immunol. 8: 1504. -
Kan, B. et al. (2018) Cellular metabolism constrains innate immune responses in early human ontogeny.
Nat Commun. 9 (1): 4822. -
de Quaglia E Silva. J.C.et al. (2019) Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis.
J Immunol Res. 2019: 1529189.
- Synonyms
- Beta Glucan Receptor
- CD369
- RRID
- AB_1658098
- UniProt
- Q9BXN2
- Entrez Gene
- CLEC7A
MCA4661GA
147758
152887
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