DAZL antibody | 3/11A
Mutations in this gene have been linked to severe spermatogenic failure and infertility in males (Lin et al. 2001)
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat Cynomolgus monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Tissue Culture Supernatant containing 0.1M Tris/HCl
- Preservative Stabilisers
0.09% Sodium Azide
- Synthetic peptide corresponding to sequence within the C terminal domain of human DAZL (CRVHHFRRSRAMLKSV).
- Fusion Partners
- Spleen cells from immunised T/O outbred mice were fused with cells of the SP2/0 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0000166 nucleotide binding
- GO:0005515 protein binding
- GO:0003723 RNA binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0007275 multicellular organismal development
- GO:0007281 germ cell development
- GO:0007283 spermatogenesis
- GO:0008494 translation activator activity
- GO:0045948 positive regulation of translational initiation
- For research purposes only
Applications of DAZL antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Ovary or testis.
Secondary Antibodies Available
Product Specific References
References for DAZL antibody
Forand, A. & Bernardino-Sgherri, J. (2009) A critical role of PUMA in maintenance of genomic integrity of murine spermatogonial stem cell precursors after genotoxic stress.
Cell Res. 19: 1018-30.
Elkin, N.D. (2010) Toxicant-induced leakage of germ cell-specific proteins from seminiferous tubules in the rat: relationship to blood-testis barrier integrity and prospects for biomonitoring.
Toxicol Sci.117: 439-48.
Barrios, F. et al. (2010) Opposing effects of retinoic acid and FGF9 on Nanos2 expression and meiotic entry of mouse germ cells.
J Cell Sci. 123: 871-80.
Forand, A. et al. (2009) Similarities and differences in the in vivo response of mouse neonatal gonocytes and spermatogonia to genotoxic stress.
Biol Reprod. 80: 860-73.
Anderson, R.A. et al. (2007) Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis.
BMC Dev Biol. 7: 136.
Aoki, T. and Takada, T. (2012) Bisphenol A modulates germ cell differentiation and retinoic acid signaling in mouse ES cells.
Reprod Toxicol. 34: 463-70.
Yamauchi K et al. (2009) In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells.
PLoS One. 4 (4): e5338.
Zogbi, C. et al. (2012) Gonocyte development in rats: proliferation, distribution and death revisited.
Histochem Cell Biol. 138 (2): 305-22.
Woods DC et al. (2013) Embryonic stem cell-derived granulosa cells participate in ovarian follicle formation in vitro and in vivo.
Reprod Sci. 20 (5): 524-35.
Rose CM et al. (2014) Dynamic changes in DNA modification states during late gestation male germ line development in the rat.
Epigenetics Chromatin. 7: 19.
Chen SR et al. (2013) Disruption of genital ridge development causes aberrant primordial germ cell proliferation but does not affect their directional migration.
BMC Biol. 11: 22.
Jobling MS et al. (2011) Effects of di(n-butyl) phthalate exposure on foetal rat germ-cell number and differentiation: identification of age-specific windows of vulnerability.
Int J Androl. 34 (5 Pt 2): e386-96.
Conrad, S. et al. (2014) Differential gene expression profiling of enriched human spermatogonia after short- and long-term culture.
Biomed Res Int. 2014: 138350.
Wang Y et al. (2015) Protein Arginine Methyltransferase 5 (Prmt5) Is Required for Germ Cell Survival During Mouse Embryonic Development.
Biol Reprod. pii: biolreprod.114.127308.
Bayne RA et al. (2015) GDF9 is Transiently Expressed in Oocytes before Follicle Formation in the Human Fetal Ovary and is Regulated by a Novel NOBOX Transcript.
PLoS One. 10 (3): e0119819.
Endo, T. et al. (2015) Periodic retinoic acid-STRA8 signaling intersects with periodic germ-cell competencies to regulate spermatogenesis.
Proc Natl Acad Sci U S A. 112 (18): E2347-56.
Tian-Zhong, M. et al. (2016) Critical role of Emx2 in the pluripotency - differentiation transition in male gonocytes via regulation of FGF9/NODAL pathway.
Reproduction. 151 (6): 673-81.
Chen, M.et al. (2019) Abnormal Meiosis Initiation in Germ Cell Caused by Aberrant Differentiation of Gonad Somatic Cell
Oxidative Medicine and Cellular Longevity. 2019: 1-8.