DAZL antibody | 3/11A

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Mouse anti DAZL

Product Type
Monoclonal Antibody
Clone
3/11A
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2336 IF P* WB 2 ml
Mouse anti Human DAZL antibody, clone 3/11A recognizes human Deleted in azoospermia-like, also known as DAZL, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1 or SPGY-like-autosomal. DAZL is a 295 amino acid ~33 kDa member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is predominantly localized to the cytoplasm.

Mutations in this gene have been linked to severe spermatogenic failure and infertility in males (Lin et al. 2001)

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.1M Tris/HCl
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Synthetic peptide corresponding to sequence within the C terminal domain of human DAZL (CRVHHFRRSRAMLKSV).
Fusion Partners
Spleen cells from immunised T/O outbred mice were fused with cells of the SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q92904 Related reagents
Entrez Gene
DAZL Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0003723 RNA binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0007275 multicellular organismal development
GO:0007281 germ cell development
GO:0007283 spermatogenesis
GO:0008494 translation activator activity
GO:0045948 positive regulation of translational initiation
Regulatory
For research purposes only

Applications of DAZL antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Ovary or testis.

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

References for DAZL antibody

  1. Forand, A. & Bernardino-Sgherri, J. (2009) A critical role of PUMA in maintenance of genomic integrity of murine spermatogonial stem cell precursors after genotoxic stress.
    Cell Res. 19: 1018-30.
  2. Elkin, N.D. (2010) Toxicant-induced leakage of germ cell-specific proteins from seminiferous tubules in the rat: relationship to blood-testis barrier integrity and prospects for biomonitoring.
    Toxicol Sci.117: 439-48.
  3. Barrios, F. et al. (2010) Opposing effects of retinoic acid and FGF9 on Nanos2 expression and meiotic entry of mouse germ cells.
    J Cell Sci. 123: 871-80.
  4. Forand, A. et al. (2009) Similarities and differences in the in vivo response of mouse neonatal gonocytes and spermatogonia to genotoxic stress.
    Biol Reprod. 80: 860-73.
  5. Anderson, R.A. et al. (2007) Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis.
    BMC Dev Biol. 7: 136.
  6. Aoki, T. and Takada, T. (2012) Bisphenol A modulates germ cell differentiation and retinoic acid signaling in mouse ES cells.
    Reprod Toxicol. 34: 463-70.
  7. Yamauchi K et al. (2009) In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells.
    PLoS One. 4 (4): e5338.
  8. Zogbi, C. et al. (2012) Gonocyte development in rats: proliferation, distribution and death revisited.
    Histochem Cell Biol. 138 (2): 305-22.
  9. Woods DC et al. (2013) Embryonic stem cell-derived granulosa cells participate in ovarian follicle formation in vitro and in vivo.
    Reprod Sci. 20 (5): 524-35.
  10. Rose CM et al. (2014) Dynamic changes in DNA modification states during late gestation male germ line development in the rat.
    Epigenetics Chromatin. 7: 19.
  11. Chen SR et al. (2013) Disruption of genital ridge development causes aberrant primordial germ cell proliferation but does not affect their directional migration.
    BMC Biol. 11: 22.
  12. Jobling MS et al. (2011) Effects of di(n-butyl) phthalate exposure on foetal rat germ-cell number and differentiation: identification of age-specific windows of vulnerability.
    Int J Androl. 34 (5 Pt 2): e386-96.
  13. Tian-Zhong, M. et al. (2016) Critical role of Emx2 in the pluripotency - differentiation transition in male gonocytes via regulation of FGF9/NODAL pathway.
    Reproduction. 151 (6): 673-81.
  14. Conrad, S. et al. (2014) Differential gene expression profiling of enriched human spermatogonia after short- and long-term culture.
    Biomed Res Int. 2014: 138350.
  15. Wang Y et al. (2015) Protein Arginine Methyltransferase 5 (Prmt5) Is Required for Germ Cell Survival During Mouse Embryonic Development.
    Biol Reprod. pii: biolreprod.114.127308.
  16. Bayne RA et al. (2015) GDF9 is Transiently Expressed in Oocytes before Follicle Formation in the Human Fetal Ovary and is Regulated by a Novel NOBOX Transcript.
    PLoS One. 10 (3): e0119819.
  17. Endo, T. et al. (2015) Periodic retinoic acid-STRA8 signaling intersects with periodic germ-cell competencies to regulate spermatogenesis.
    Proc Natl Acad Sci U S A. 112 (18): E2347-56.