Complement Factor I antibody | 052.11.3 (3R/8)
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Mouse anti Human Complement Factor I antibody, clone 052.11.3 (3R/8) recognizes complement factor I, a member of the peptidase S1 family present in the blood plasma. Factor I is an 88 kDa protein consisting of one polypeptide chain of 35.4 kDa and one of 27.6 kDa. Factor I is a serine protease which inactivates complement components C3b and C4b. C3b cleavage requires the presence of cofactors complement component (3b/4b) receptor 1 (CR1), membrane cofactor protein or factor H, while C4b cleavage requires complement component 4 binding protein or CR1. Factor I has no natural inhibitors.
Dysfunctional factor I can lead to uncontrolled activation of the alternative complement pathway causing in systemic depletion of C3 and lower levels of factors B and H, resulting in recurrent pyogenic infections. Defects in CFI are also associated with haemolytic uraemic syndrome.
Mouse anti Human Complement Factor I antibody, clone 3R/8 blocks function of complement factor I.
Removal of Sodium Azide is recommended prior to use in functional assays.
Dysfunctional factor I can lead to uncontrolled activation of the alternative complement pathway causing in systemic depletion of C3 and lower levels of factors B and H, resulting in recurrent pyogenic infections. Defects in CFI are also associated with haemolytic uraemic syndrome.
Mouse anti Human Complement Factor I antibody, clone 3R/8 blocks function of complement factor I.
Removal of Sodium Azide is recommended prior to use in functional assays.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Native human factor I from serum.
- Approx. Protein Concentrations
- IgG concentration 1.14 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P05156
- Entrez Gene
- CFI
- GO Terms
- GO:0016020 membrane
- GO:0004252 serine-type endopeptidase activity
- GO:0005044 scavenger receptor activity
- GO:0005615 extracellular space
- GO:0006508 proteolysis
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
- Regulatory
- For research purposes only
Applications of Complement Factor I antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | 1/4000 | |
| Flow Cytometry | |||
| Functional Assays | |||
| Immunohistology - Frozen | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from post streptoccal glomerulonephritis patients
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for Complement Factor I antibody
-
Dragon-Durey, M.A. & Frémeaux-Bacchi, V. (2005) Atypical haemolytic uraemic syndrome and mutations in complement regulator genes.
Springer Semin Immunopathol. 27 (3): 359-74. -
Nilsson, S.C. et al. (2007) A mutation in factor I that is associated with atypical hemolytic uremic syndrome does not affect the function of factor I in complement regulation.
Mol Immunol. 44 (8): 1835-44.
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