Complement Factor I antibody | 052.11.3 (3R/8)
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Mouse anti Human Complement Factor I
- Product Type
- Monoclonal Antibody
- Clone
- 052.11.3 (3R/8)
- Isotype
- IgG1
- Specificity
- Complement Factor I
Antibody Quality and Performance Guarantee
| Mouse anti Human Complement Factor I antibody, clone 052.11.3 (3R/8) recognizes complement factor I, a member of the peptidase S1 family present in the blood plasma. Factor I is an 88 kDa protein consisting of one polypeptide chain of 35.4 kDa and one of 27.6 kDa. Factor I is a serine protease which inactivates complement components C3b and C4b. C3b cleavage requires the presence of cofactors complement component (3b/4b) receptor 1 (CR1), membrane cofactor protein or factor H, while C4b cleavage requires complement component 4 binding protein or CR1. Factor I has no natural inhibitors. Dysfunctional factor I can lead to uncontrolled activation of the alternative complement pathway causing in systemic depletion of C3 and lower levels of factors B and H, resulting in recurrent pyogenic infections. Defects in CFI are also associated with haemolytic uraemic syndrome. Mouse anti Human Complement Factor I antibody, clone 3R/8 blocks function of complement factor I. Removal of Sodium Azide is recommended prior to use in functional assays. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Native human factor I from serum.
- Approx. Protein Concentrations
- Current, batch-specific concentration 1.20 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/500 | 1/4000 |
| Flow Cytometry | |
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| Functional Assays | |
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| Immunohistology - Frozen | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from post streptoccal glomerulonephritis patients
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for Complement Factor I antibody
-
Dragon-Durey, M.A. & Frémeaux-Bacchi, V. (2005) Atypical haemolytic uraemic syndrome and mutations in complement regulator genes.
Springer Semin Immunopathol. 27 (3): 359-74. -
Nilsson, S.C. et al. (2007) A mutation in factor I that is associated with atypical hemolytic uremic syndrome does not affect the function of factor I in complement regulation.
Mol Immunol. 44 (8): 1835-44.
- RRID
- AB_844540
- UniProt
- P05156
- Entrez Gene
- CFI
- GO Terms
- GO:0016020 membrane
- GO:0004252 serine-type endopeptidase activity
- GO:0005044 scavenger receptor activity
- GO:0005615 extracellular space
- GO:0006508 proteolysis
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
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