Complement Factor H antibody | OX-24

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Mouse anti Human Complement Factor H

Product Type
Monoclonal Antibody
Clone
OX-24
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA509G E IP WB 0.2 mg
Mouse anti Human Complement Factor H antibody, clone OX-24 recognizes the human serum complement protein factor H (155 kDa), binding to a different epitope from MRC OX23. The antibody also recognizes a 43-49 kDa truncated form of factor H present at low level (1-5 μg/ml) in plasma and urine. In an immunoprecipitation system, it recognizes factor H in other primates, but not in cow, sheep, pig, chicken or rabbit sera.

Mouse anti Human Complement Factor H antibody, clone OX-24 inhibits the binding of factor H to surface bound C3b, and recognizes Factor H in Western blotting procedures.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human complement factor H.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
NS-0 myeloma fused with spleen cells from BALB/c mice.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P08603 Related reagents
Entrez Gene
CFH Related reagents
Regulatory
For research purposes only

Applications of Complement Factor H antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Complement Factor H antibody

  1. Alsenz, J. et al. (1985) Structural and functional analysis of the complement component factor H with the use of different enzymes and monoclonal antibodies to factor H.
    Biochem J. 232 (3): 841-50.
  2. Johnson, S.A. et al. (2010) Impact of compound heterozygous complement factor H mutations on development of atypical hemolytic uremic syndrome-A pedigree revisited.
    Mol Immunol. 47 (7-8): 1585-91.
  3. Kelly, U. et al. (2010) Heparan Sulfate, Including That in Bruch's Membrane, Inhibits the Complement Alternative Pathway: Implications for Age-Related Macular Degeneration.
    J Immunol. 185: 5486-94.
  4. Fontaine, M. et al. (1989) Truncated forms of human complement factor H.
    Biochem J. 258 (3): 927-30.
  5. Sim, E. et al. (1983) Monoclonal antibodies against the complement control protein factor H (beta 1 H).
    Biosci Rep. 3 (12): 1119-31.
  6. Hamidov, Z. et al. (2011) Reduced expression of desmocollin 2 is an independent prognostic biomarker for shorter patients survival in pancreatic ductal adenocarcinoma.
    J Clin Pathol. 64 (11): 990-4.