Complement Factor H antibody | OX-23
Mouse anti Human factor H antibody, clone OX-23 (MCA508G) used as a capture reagent in a sandwich ELISA for HfH levels in murine sera with Sheep anti Human complement factor H antibody (4400-9504) as a detection reagent
Image caption:
Effect of the presence of hfH in mice on the nOMV immunogenicity.
A. Evolution of hfH concentration in mouse serum over time after two IP injection of 0.5mg purified hfH (black arrows) 24 and 0.5 hours prior to immunization (grey arrow). B. Serum Bactericidal Assay (SBA) titers against 44/76-SL wild-type in pooled sera from C57bl/6 mice pre-injected with 800 μg/ml hfH or hSA, 14 days following immunization with 5 &mu:g of nOMVlpxl1 or nOMVdis. C. Individual SBA titers against 44/76-SL, dots represent individual animals D. Individual ELISA titers against rfHbp induced after one dose of H44/76Lpxl1 OMV in mice pre-injected with hfH or hSA, *p = 0.016, not all sera were available. C, D dots represent individual animals, the geometric mean and 95% confidence interval are represented as horizontal bars.
From: Daniels-Treffandier H, de Nie K, Marsay L, Dold C, Sadarangani M, Reyes-Sandoval A, et al. (2016)
Impact of Reducing Complement Inhibitor Binding on the Immunogenicity of Native Neisseria meningitidis Outer Membrane Vesicles.
PLoS ONE 11(2): e0148840.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human complement factor H antibody, clone OX-23 (MCA508G) as a detection antibody in a sandwich ELISA for the detection of free factor H in human plasma samples.
Image caption:
Insert image caption
From: Puraswani, M. et al.
Clinical and Immunological Profile of Anti-factor H Antibody Associated Atypical Hemolytic Uremic Syndrome: A Nationwide Database.
Front Immunol. 2019 Jun 7;10:1282.
doi: 10.3389/fimmu.2019.01282.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Rabbit Bovine Chicken Primate Pig Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
Applications of Complement Factor H antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/50 | 1/200 | |
| Immunoprecipitation | |||
| Radioimmunoassays | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Complement Factor H antibody
-
Sim, E. et al. (1983) Monoclonal antibodies against the complement control protein factor H (beta 1 H).
Biosci Rep. 3 (12): 1119-31. -
Alsenz, J. et al. (1985) Structural and functional analysis of the complement component factor H with the use of different enzymes and monoclonal antibodies to factor H.
Biochem J. 232 (3): 841-50. -
Fontaine, M. et al. (1989) Truncated forms of human complement factor H.
Biochem J. 258 (3): 927-30. -
Daniels-Treffandier, H. et al. (2016) Impact of Reducing Complement Inhibitor Binding on the Immunogenicity of Native Neisseria meningitidis Outer Membrane Vesicles.
PLoS One. 11 (2): e0148840. -
Kelly, U. et al. (2010) Heparan sulfate, including that in Bruch's membrane, inhibits the complement alternative pathway: implications for age-related macular degeneration.
J Immunol. 185 (9): 5486-94. -
Smith J et al. (2007) Genes expressed by both mesangial cells and bone marrow-derived cells underlie genetic susceptibility to crescentic glomerulonephritis in the rat.
J Am Soc Nephrol. 18 (6): 1816-23. -
Clark, S.J. et al. (2014) Identification of factor H-like protein 1 as the predominant complement regulator in Bruch's membrane: implications for age-related macular degeneration.
J Immunol. 193 (10): 4962-70. -
Martínez, A. et al. (2003) Mapping of the Adrenomedullin-Binding Domains in Human Complement Factor H.
Hypertension Research. 26 (Suppl): S55-S59. -
Puraswani, M. et al. (2019) Clinical and Immunological Profile of Anti-factor H Antibody Associated Atypical Hemolytic Uremic Syndrome: A Nationwide Database.
Front Immunol. 10: 1282.
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