Complement Factor H antibody | OX-23

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Mouse anti Human Complement Factor H

Product Type
Monoclonal Antibody
Clone
OX-23
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA508G E IP R WB 0.2 mg
Mouse anti Human Complement Factor H antibody, clone OX-23 recognizes intact human serum complement protein factor H, also known as H factor 1. Complement factor H is a 1213 amino acid ~155 kDa secreted glycoprotein bearing multiple disulphide bonds and is involved in the deactivation of C3b and dissociation of C3 convertase in the alternative complement pathway. Mouse anti Human Complement Factor H antibody, clone OX-23 also recognizes a ~43-49 kDa truncated form of factor H present at low level (1-5ug/ml) in plasma and urine.

Mutations in the CFH gene can lead to the development of Complement Factor H deficiency (CFHD) which can be asymptomatic, present with recurrent bacterial infections or renal failure (Ault et al. 1997). Mutations can also lead to development of Basal laminar drusen (BLD), the deposition of extracellular deposits accumulating below the retinal pigment epithelium on Bruch membrane which can ultimately lead to vision loss (Boon et al. 2008). Additionally, mutations in the CFH gene can lead to increased susceptibility to Hemolytic uremic syndrome atypical 1 (AHUS1) or Macular degeneration, age-related, 4 (ARMD4).

Mouse anti Human Complement Factor H antibody, clone OX-23 has been used successfully for the determination of levels of bound murine factor H in a sandwich ELISA in conjunction with Sheep anti Human Factor H antibody (4400-9504 ) as a capture reagent (Daniels-Treffandier et al. 2016).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Rabbit
Bovine
Chicken
Primate
Pig
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human complement factor H.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS-O mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P08603 Related reagents
Entrez Gene
CFH Related reagents
Regulatory
For research purposes only

Applications of Complement Factor H antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50 1/200
Immunoprecipitation
Radioimmunoassays
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Application Based External Images

Western Blotting

Product Specific References

References for Complement Factor H antibody

  1. Sim, E. et al. (1983) Monoclonal antibodies against the complement control protein factor H (beta 1 H).
    Biosci Rep. 3 (12): 1119-31.
  2. Alsenz, J. et al. (1985) Structural and functional analysis of the complement component factor H with the use of different enzymes and monoclonal antibodies to factor H.
    Biochem J. 232 (3): 841-50.
  3. Fontaine, M. et al. (1989) Truncated forms of human complement factor H.
    Biochem J. 258 (3): 927-30.
  4. Daniels-Treffandier, H. et al. (2016) Impact of Reducing Complement Inhibitor Binding on the Immunogenicity of Native Neisseria meningitidis Outer Membrane Vesicles.
    PLoS One. 11 (2): e0148840.
  5. Kelly, U. et al. (2010) Heparan sulfate, including that in Bruch's membrane, inhibits the complement alternative pathway: implications for age-related macular degeneration.
    J Immunol. 185 (9): 5486-94.
  6. Smith J et al. (2007) Genes expressed by both mesangial cells and bone marrow-derived cells underlie genetic susceptibility to crescentic glomerulonephritis in the rat.
    J Am Soc Nephrol. 18 (6): 1816-23.
  7. Clark, S.J. et al. (2014) Identification of factor H-like protein 1 as the predominant complement regulator in Bruch's membrane: implications for age-related macular degeneration.
    J Immunol. 193 (10): 4962-70.
  8. Martínez, A. et al. (2003) Mapping of the Adrenomedullin-Binding Domains in Human Complement Factor H
    Hypertension Research. 26 (Suppl): S55-S59.