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Chymotrypsin antibody | CHYMO10-104.5 (4E1)

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Anti Human Chymotrypsin Antibody, clone CHYMO10-104.5 (4E1) thumbnail image 1 Anti Human Chymotrypsin Antibody, clone CHYMO10-104.5 (4E1) thumbnail image 2
Anti Human Chymotrypsin Antibody, clone CHYMO10-104.5 (4E1) gallery image 1
Published customer image:
Mouse anti Human chymotrypsin antibody, clone 4E1 (2100-0657) used for the detection of chymotrypsin in mouse tissues by immunohistochemistry
Image caption:
Endocrine and exocrine compartments destruction in the pancreas of Pdx1-Cre;LSL-KrasG12D;Tif1γlox/lox mice. Wild-type (A–C') and Pdx1-Cre; LSL-KrasG12D; Tif1γlox/lox (D–L') pancreas sections were stained with HPS (A,D,G,J), immuno-revealed either with an anti-chymotrypsin antibody (B,E,H,K) or an anti-insulin antibody (C,F,I,L and C',F',I',L' that represent the boxed region in C,F,I,L at a higher magnification). For Pdx1-Cre;LSL-KrasG12D;Tif1γlox/lox mice, analysis were performed at different ages. Number of mice: 3 weeks, n = 3; 6–13 weeks, n = 5; 18–27 weeks, n = 4. Boxes: Higher magnification. The pictures show one representative mouse for each group.

From: Vincent DF, Yan K-P, Treilleux I, Gay F, Arfi V, Kaniewsky B, et al. (2009) Inactivation of TIF1γ Cooperates with KrasG12D to Induce Cystic Tumors of the Pancreas.
PLoS Genet 5(7): e1000575.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Chymotrypsin Antibody, clone CHYMO10-104.5 (4E1) gallery image 2
Published customer image:
Mouse anti Human chymotrypsin antibody, clone 4E1 (2100-0657) used for the demonstration of chymotrypsin expression in human pancreas by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
The cyst-lining acinar cells contain apical granules Both cyst-lining acinar cells and nondescriptive flattened/cuboidal epithelium are positive for chymotrypsin (e&f, immunoperoxidase stain, 200x and 100x, respectively; f, non-descriptive epithelium marked by arrow).

From: Multilocular Pancreatic Acinar Cystadenoma Containing Areas of Multifocal Branch-Duct Intraductal Papillary Mucinous Neoplasm.
J Pancreas 16(5): 475-80.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human Chymotrypsin

Product Type
Monoclonal Antibody
Clone
CHYMO10-104.5 (4E1)
Isotype
IgG3
Product Code Applications Pack Size List Price Quantity
2100-0657
Datasheet
C E IF P WB
SDS
0.2 mg loader

Mouse anti Human chymotrypsin antibody, clone 4E1 recognizes human chymotrypsin-C, also known as chymotrypsin or caldecrin. Chymotrypsin is a 239 amino acid ~30 kDa protease with an additional 13 amino acid propeptide region and a 16 amino acid signal peptide.

Variations in the CTRC gene has been associated with susceptibility to hereditary, pancreatitis (PCTT), a disease characterized by pancreatic inflammation and destruction of the parenchyma (Beer et al. 2013).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Purified human pancreatic chymotrypsin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q99895
Entrez Gene
CTRC
GO Terms
GO:0004252 serine-type endopeptidase activity
GO:0006508 proteolysis
Regulatory
For research purposes only

Applications of Chymotrypsin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG3:FITC HCA039F F 0.1 mg loader
Human anti Mouse IgG3:HRP HCA039P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for Chymotrypsin antibody

  1. Jimenez, R.E. et al. (1999) Immunohistochemical characterization of pancreatic tumors induced by dimethylbenzanthracene in rats.
    Am J Pathol. 154 (4): 1223-9.
  2. Larina, O.et al. (2007) Dynamic regulation of the large exocytotic fusion pore in pancreatic acinar cells.
    Mol Biol Cell. 18:3502-11.
  3. Bockman, D.E. et al. (2003) Origin and development of the precursor lesions in experimental pancreatic cancer in rats.
    Lab Invest. 83 (6): 853-9.
  4. Li, H. et al. (2009) The Ink4/Arf locus is a barrier for iPS cell reprogramming.
    Nature. 460: 1136-9.
  5. Vincent, D.F. et al. (2009) Inactivation of TIF1gamma cooperates with Kras to induce cystic tumors of the pancreas.
    PLoS Genet. 5: e1000575.
  6. Behrendorff, N. et al. (2011) Vesicle-associated membrane protein 8 (VAMP8) is a SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) selectively required for sequential granule-to-granule fusion.
    J Biol Chem. 286 (34): 29627-34.
  7. Guerra, C. et al. (2011) Pancreatitis-induced inflammation contributes to pancreatic cancer by inhibiting oncogene-induced senescence.
    Cancer Cell. 19: 728-39.
  8. Kanayama, K. et al. (2016) Cytological findings of an ectopic pancreas of the stomach obtained at endoscopic ultrasound-guided fine needle aspiration, differential diagnosis from acinar cell carcinoma: a case report.
    Cytopathology. 27 (5): 379-81.
  9. Vincent DF et al. (2012) Tif1γ suppresses murine pancreatic tumoral transformation by a Smad4-independent pathway.
    Am J Pathol. 180 (6): 2214-21.
  10. Kato, Y. et al. (2014) Ectopic tissue consisting of a mixture of glandular gastric, intestinal, and exocrine pancreatic tissue in the forestomach of a rat.
    J Toxicol Pathol. 27 (1): 87-90.
  11. Bansal, S. et al.. (2015) Multilocular Pancreatic Acinar Cystadenoma Containing Areas of Multifocal Branch-Duct Intraductal Papillary Mucinous Neoplasm
    J. Pancreas 15 (5): 475-80.

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