Chymotrypsin antibody | CHYMO10-104.5 (4E1)
Mouse anti Human chymotrypsin antibody, clone 4E1 (2100-0657) used for the detection of chymotrypsin in mouse tissues by immunohistochemistry
Image caption:
Endocrine and exocrine compartments destruction in the pancreas of Pdx1-Cre;LSL-KrasG12D;Tif1γlox/lox mice. Wild-type (A–C') and Pdx1-Cre; LSL-KrasG12D; Tif1γlox/lox (D–L') pancreas sections were stained with HPS (A,D,G,J), immuno-revealed either with an anti-chymotrypsin antibody (B,E,H,K) or an anti-insulin antibody (C,F,I,L and C',F',I',L' that represent the boxed region in C,F,I,L at a higher magnification). For Pdx1-Cre;LSL-KrasG12D;Tif1γlox/lox mice, analysis were performed at different ages. Number of mice: 3 weeks, n = 3; 6–13 weeks, n = 5; 18–27 weeks, n = 4. Boxes: Higher magnification. The pictures show one representative mouse for each group.
From: Vincent DF, Yan K-P, Treilleux I, Gay F, Arfi V, Kaniewsky B, et al. (2009) Inactivation of TIF1γ Cooperates with KrasG12D to Induce Cystic Tumors of the Pancreas.
PLoS Genet 5(7): e1000575.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human chymotrypsin antibody, clone 4E1 (2100-0657) used for the demonstration of chymotrypsin expression in human pancreas by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
The cyst-lining acinar cells contain apical granules Both cyst-lining acinar cells and nondescriptive flattened/cuboidal epithelium are positive for chymotrypsin (e&f, immunoperoxidase stain, 200x and 100x, respectively; f, non-descriptive epithelium marked by arrow).
From: Multilocular Pancreatic Acinar Cystadenoma Containing Areas of Multifocal Branch-Duct Intraductal Papillary Mucinous Neoplasm.
J Pancreas 16(5): 475-80.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human chymotrypsin antibody, clone 4E1 recognizes human chymotrypsin-C, also known as chymotrypsin or caldecrin. Chymotrypsin is a 239 amino acid ~30 kDa protease with an additional 13 amino acid propeptide region and a 16 amino acid signal peptide.
Variations in the CTRC gene has been associated with susceptibility to hereditary, pancreatitis (PCTT), a disease characterized by pancreatic inflammation and destruction of the parenchyma (Beer et al. 2013).
Variations in the CTRC gene has been associated with susceptibility to hereditary, pancreatitis (PCTT), a disease characterized by pancreatic inflammation and destruction of the parenchyma (Beer et al. 2013).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Purified human pancreatic chymotrypsin.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q99895
- Entrez Gene
- CTRC
- GO Terms
- GO:0004252 serine-type endopeptidase activity
- GO:0006508 proteolysis
- Regulatory
- For research purposes only
Applications of Chymotrypsin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Application Based External Images
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Product Specific References
References for Chymotrypsin antibody
-
Jimenez, R.E. et al. (1999) Immunohistochemical characterization of pancreatic tumors induced by dimethylbenzanthracene in rats.
Am J Pathol. 154 (4): 1223-9. -
Larina, O.et al. (2007) Dynamic regulation of the large exocytotic fusion pore in pancreatic acinar cells.
Mol Biol Cell. 18:3502-11. -
Bockman, D.E. et al. (2003) Origin and development of the precursor lesions in experimental pancreatic cancer in rats.
Lab Invest. 83 (6): 853-9. -
Li, H. et al. (2009) The Ink4/Arf locus is a barrier for iPS cell reprogramming.
Nature. 460: 1136-9. -
Vincent, D.F. et al. (2009) Inactivation of TIF1gamma cooperates with Kras to induce cystic tumors of the pancreas.
PLoS Genet. 5: e1000575. -
Behrendorff, N. et al. (2011) Vesicle-associated membrane protein 8 (VAMP8) is a SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) selectively required for sequential granule-to-granule fusion.
J Biol Chem. 286 (34): 29627-34. -
Guerra, C. et al. (2011) Pancreatitis-induced inflammation contributes to pancreatic cancer by inhibiting oncogene-induced senescence.
Cancer Cell. 19: 728-39. -
Kanayama, K. et al. (2016) Cytological findings of an ectopic pancreas of the stomach obtained at endoscopic ultrasound-guided fine needle aspiration, differential diagnosis from acinar cell carcinoma: a case report.
Cytopathology. 27 (5): 379-81. -
Vincent DF et al. (2012) Tif1γ suppresses murine pancreatic tumoral transformation by a Smad4-independent pathway.
Am J Pathol. 180 (6): 2214-21. -
Kato, Y. et al. (2014) Ectopic tissue consisting of a mixture of glandular gastric, intestinal, and exocrine pancreatic tissue in the forestomach of a rat.
J Toxicol Pathol. 27 (1): 87-90. -
Bansal, S. et al.. (2015) Multilocular Pancreatic Acinar Cystadenoma Containing Areas of Multifocal Branch-Duct Intraductal Papillary Mucinous Neoplasm
J. Pancreas 15 (5): 475-80.
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