Cftr antibody | M3A7
Mouse anti Human Cystic Fibrosis Transmembrane Conductance Regulator
- Product Type
- Monoclonal Antibody
|Mouse anti Human Cystic Fibrosis Transmembrane Conductance Regulator antibody, clone M3A7 recognizes the human cystic fibrosis transmembrane conductance regulator (CFTR). It recognises an epitope localizing to the ABC transporter 2 domain, specifically to a region between amino acids 1370 to 1380.|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antibody purified from ascites
- Buffer Solution
- Phosphate Buffered Saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Fusion protein containing residues 1197-1480 of human CFTR (Chang et al. 1993).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Protein Molecular Weight
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1|
|Immunohistology - Paraffin|
|Western Blotting 2||10ug/ml|
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2This antibody recognises a 170kDa band under native conditions.
References for Cftr antibody
Kartner, N. et al. (1992) Mislocalization of delta F508 CFTR in cystic fibrosis sweat gland.
Nat Genet. 1 (5): 321-7.
Kartner, N. & Riordan, J.R. (1998) Characterization of polyclonal and monoclonal antibodies to cystic fibrosis transmembrane conductance regulator.
Methods Enzymol. 292: 629-52.
Chang, X.B. et al. (1993) Protein kinase A (PKA) still activates CFTR chloride channel after mutagenesis of all 10 PKA consensus phosphorylation sites.
J Biol Chem. 268 (15): 11304-11.
Zhang, F. et al. (1998) Limited proteolysis as a probe for arrested conformational maturation of delta F508 CFTR.
Nat Struct Biol. 5 (3): 180-3.
Benharouga, M. et al. (2001) COOH-terminal truncations promote proteasome-dependent degradation of mature cystic fibrosis transmembrane conductance regulator from post-Golgi compartments.
J Cell Biol. 153: 957-70.
- Entrez Gene
- GO Terms
- GO:0005224 ATP-binding and phosphorylation-dependent chloride channel activity
- GO:0005260 channel-conductance-controlling ATPase activity
- GO:0005524 ATP binding
- GO:0007585 respiratory gaseous exchange
- GO:0016323 basolateral plasma membrane
- GO:0016324 apical plasma membrane
- GO:0017081 chloride channel regulator activity
- GO:0019899 enzyme binding
- GO:0030165 PDZ domain binding
- View More GO Terms
- GO:0031901 early endosome membrane
- GO:0034707 chloride channel complex
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