Mouse anti Human Cystic Fibrosis Transmembrane Conductance Regulator antibody, clone M3A7 recognizes the human cystic fibrosis transmembrane conductance regulator (CFTR). It recognises an epitope localizing to the ABC transporter 2 domain, specifically to a region between amino acids 1370 to 1380.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate Buffered Saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Fusion protein containing residues 1197-1480 of human CFTR (Chang et al. 1993).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Protein Molecular Weight
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
ATP-binding and phosphorylation-dependent chloride channel activity
channel-conductance-controlling ATPase activity
respiratory gaseous exchange
basolateral plasma membrane
apical plasma membrane
chloride channel regulator activity
PDZ domain binding
early endosome membrane
chloride channel complex
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Cftr antibody
|Immunohistology - Frozen 1
|Immunohistology - Paraffin
|Western Blotting 2
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2This antibody recognises a 170kDa band under native conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Application Based External Images
Product Specific References
References for Cftr antibody
Kartner, N. et al. (1992) Mislocalization of delta F508 CFTR in cystic fibrosis sweat gland.
Nat Genet. 1 (5): 321-7.
Kartner, N. & Riordan, J.R. (1998) Characterization of polyclonal and monoclonal antibodies to cystic fibrosis transmembrane conductance regulator.
Methods Enzymol. 292: 629-52.
Chang, X.B. et al. (1993) Protein kinase A (PKA) still activates CFTR chloride channel after mutagenesis of all 10 PKA consensus phosphorylation sites.
J Biol Chem. 268 (15): 11304-11.
Zhang, F. et al. (1998) Limited proteolysis as a probe for arrested conformational maturation of delta F508 CFTR.
Nat Struct Biol. 5 (3): 180-3.
Benharouga, M. et al. (2001) COOH-terminal truncations promote proteasome-dependent degradation of mature cystic fibrosis transmembrane conductance regulator from post-Golgi compartments.
J Cell Biol. 153: 957-70.
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