Cftr antibody | M3A7

Mouse anti Human Cystic Fibrosis Transmembrane Conductance Regulator

Product Type
Monoclonal Antibody
Clone
M3A7
Isotype
IgG1
Specificity
Cftr

Product Code Applications Pack Size List Price Your Price Qty
OBT1680
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * IP WB * 0.1 mg loader
List Price Your Price
loader
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Mouse anti Human Cystic Fibrosis Transmembrane Conductance Regulator antibody, clone M3A7 recognizes the human cystic fibrosis transmembrane conductance regulator (CFTR). It recognises an epitope localizing to the ABC transporter 2 domain, specifically to a region between amino acids 1370 to 1380.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antibody purified from ascites
Buffer Solution
Phosphate Buffered Saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Fusion protein containing residues 1197-1480 of human CFTR (Chang et al. 1993).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Protein Molecular Weight
170kDA
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting 2 10ug/ml
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  2. 2This antibody recognises a 170kDa band under native conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Cftr antibody

  1. Kartner, N. et al. (1992) Mislocalization of delta F508 CFTR in cystic fibrosis sweat gland.
    Nat Genet. 1 (5): 321-7.
  2. Kartner, N. & Riordan, J.R. (1998) Characterization of polyclonal and monoclonal antibodies to cystic fibrosis transmembrane conductance regulator.
    Methods Enzymol. 292: 629-52.
  3. Chang, X.B. et al. (1993) Protein kinase A (PKA) still activates CFTR chloride channel after mutagenesis of all 10 PKA consensus phosphorylation sites.
    J Biol Chem. 268 (15): 11304-11.
  4. Zhang, F. et al. (1998) Limited proteolysis as a probe for arrested conformational maturation of delta F508 CFTR.
    Nat Struct Biol. 5 (3): 180-3.
  5. Benharouga, M. et al. (2001) COOH-terminal truncations promote proteasome-dependent degradation of mature cystic fibrosis transmembrane conductance regulator from post-Golgi compartments.
    J Cell Biol. 153: 957-70.

Western Blotting

RRID
AB_617226
UniProt
P13569
Entrez Gene
CFTR
GO Terms
GO:0005224 ATP-binding and phosphorylation-dependent chloride channel activity
GO:0005260 channel-conductance-controlling ATPase activity
GO:0005524 ATP binding
GO:0007585 respiratory gaseous exchange
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0017081 chloride channel regulator activity
GO:0019899 enzyme binding
GO:0030165 PDZ domain binding
GO:0031901 early endosome membrane
GO:0034707 chloride channel complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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