Cftr antibody | M3A7

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Mouse anti Human Cystic Fibrosis Transmembrane Conductance Regulator

Product Type: Monoclonal Antibody
Clone: M3A7
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
OBT1680	C* IP WB*			0.1 mg

Mouse anti Human Cystic Fibrosis Transmembrane Conductance Regulator antibody, clone M3A7 recognizes the human cystic fibrosis transmembrane conductance regulator (CFTR). It recognises an epitope localizing to the ABC transporter 2 domain, specifically to a region between amino acids 1370 to 1380.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Buffer Solution

Phosphate Buffered Saline

Preservative Stabilisers

0.1% Sodium Azide (NaN₃)

Immunogen

Fusion protein containing residues 1197-1480 of human CFTR (Chang et al. 1993).

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Protein Molecular Weight

170kDA

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P13569 Related reagents
Entrez Gene: CFTR Related reagents
GO Terms: GO:0005224 ATP-binding and phosphorylation-dependent chloride channel activity; GO:0005260 channel-conductance-controlling ATPase activity; GO:0005524 ATP binding; GO:0007585 respiratory gaseous exchange; GO:0016323 basolateral plasma membrane; GO:0016324 apical plasma membrane; GO:0017081 chloride channel regulator activity; GO:0019899 enzyme binding; GO:0030165 PDZ domain binding; GO:0031901 early endosome membrane; GO:0034707 chloride channel complex
Regulatory: For research purposes only

Applications of Cftr antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen ¹
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting ²		10ug/ml

¹The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
²This antibody recognises a 170kDa band under native conditions.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Western Blotting

Product Specific References

References for Cftr antibody

Kartner, N. et al. (1992) Mislocalization of delta F508 CFTR in cystic fibrosis sweat gland.
Nat Genet. 1 (5): 321-7.
Kartner, N. & Riordan, J.R. (1998) Characterization of polyclonal and monoclonal antibodies to cystic fibrosis transmembrane conductance regulator.
Methods Enzymol. 292: 629-52.
Chang, X.B. et al. (1993) Protein kinase A (PKA) still activates CFTR chloride channel after mutagenesis of all 10 PKA consensus phosphorylation sites.
J Biol Chem. 268 (15): 11304-11.
Zhang, F. et al. (1998) Limited proteolysis as a probe for arrested conformational maturation of delta F508 CFTR.
Nat Struct Biol. 5 (3): 180-3.
Benharouga, M. et al. (2001) COOH-terminal truncations promote proteasome-dependent degradation of mature cystic fibrosis transmembrane conductance regulator from post-Golgi compartments.
J Cell Biol. 153: 957-70.

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