Cftr antibody | M3A7
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Mouse anti Human Cystic Fibrosis Transmembrane Conductance Regulator
- Product Type
- Monoclonal Antibody
- Clone
- M3A7
- Isotype
- IgG1
- Specificity
- Cftr
Antibody Quality and 
Unrivaled 
| Mouse anti Human Cystic Fibrosis Transmembrane Conductance Regulator antibody, clone M3A7 recognizes the human cystic fibrosis transmembrane conductance regulator (CFTR). It recognises an epitope localizing to the ABC transporter 2 domain, specifically to a region between amino acids 1370 to 1380. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from ascites
- Buffer Solution
- Phosphate Buffered Saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Fusion protein containing residues 1197-1480 of human CFTR (Chang et al. 1993).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Protein Molecular Weight
- 170kDA
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen 1 |  |
||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |
||
| Western Blotting 2 | |
10ug/ml |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2This antibody recognises a 170kDa band under native conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Cftr antibody
-
Kartner, N. et al. (1992) Mislocalization of delta F508 CFTR in cystic fibrosis sweat gland.
Nat Genet. 1 (5): 321-7. -
Kartner, N. & Riordan, J.R. (1998) Characterization of polyclonal and monoclonal antibodies to cystic fibrosis transmembrane conductance regulator.
Methods Enzymol. 292: 629-52. -
Chang, X.B. et al. (1993) Protein kinase A (PKA) still activates CFTR chloride channel after mutagenesis of all 10 PKA consensus phosphorylation sites.
J Biol Chem. 268 (15): 11304-11. -
Zhang, F. et al. (1998) Limited proteolysis as a probe for arrested conformational maturation of delta F508 CFTR.
Nat Struct Biol. 5 (3): 180-3. -
Benharouga, M. et al. (2001) COOH-terminal truncations promote proteasome-dependent degradation of mature cystic fibrosis transmembrane conductance regulator from post-Golgi compartments.
J Cell Biol. 153: 957-70.
- RRID
- AB_617226
- UniProt
- P13569
- Entrez Gene
- CFTR
- GO Terms
- GO:0005224 ATP-binding and phosphorylation-dependent chloride channel activity
- GO:0005260 channel-conductance-controlling ATPase activity
- GO:0005524 ATP binding
- GO:0007585 respiratory gaseous exchange
- GO:0016323 basolateral plasma membrane
- GO:0016324 apical plasma membrane
- GO:0017081 chloride channel regulator activity
- GO:0019899 enzyme binding
- GO:0030165 PDZ domain binding
- View More GO Terms
- GO:0031901 early endosome membrane
- GO:0034707 chloride channel complex
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