CD95 antibody | LOB 3/17
Mouse anti Human CD95 antibody, clone LOB 3/17 (MCA1539) used for the evaluation of CD95 expression on HUT78 T cell lymphoma cells by flow cytometry
Image caption:
Soluble CD154 inhibits Fas-mediated cell death in HUT78 T cells.
(A, Left and middle panels) HUT78 cells were incubated with anti-α5β1 JBS5 mAb, anti-CD40 G28.5 mAb, anti-Fas LOB3/17 mAb, or IgG isotype control (IC) followed by mouse anti-human Ig labeled with Alexa 488. (A, Right panel) Cells were incubated with Avidin-Alexa 488 (negative control) or sCD154-Alexa 488 (20ng/sample). (B, Left panel) HUT78 T cells were left non-stimulated (NS) or stimulated with different concentrations of anti-Fas CH-11 mAb or IgM isotype control (IC) for 48 hours at 37°C and assessed for their cell death response by FACS analysis as the percentage of death obtained by propidium iodide staining. (B, Right panel) HUT78 cells were pre-treated with different concentrations of sCD154, then left non-stimulated (NS) or stimulated with the anti-Fas CH-11 mAb (200ng/ml) for 48 h at 37°C. Cell death was then evaluated as above (± SD, * p <0.05, ** p <0.01, *** p <0.001 CH-11 versus IC).
From: Bachsais M, Naddaf N, Yacoub D, Salti S, Alaaeddine N, Aoudjit F, et al. (2016)
The Interaction of CD154 with the α5β1 Integrin Inhibits Fas-Induced T Cell Death.
PLoS ONE 11(7): e0158987.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD95 antibody, clone LOB 3/17 recognizes the human CD95 cell surface antigen, also known as Tumor necrosis factor receptor superfamily member 6, Fas, Apo-1 antigen, Apoptosis-mediating surface antigen FAS or FASLG receptor. CD95 is a 310 amino acid ~40-50 kDa single pass type I transmembrane glycoprotein expressed by activated T and B cells, NK cells and thymocytes. Mutations in the CD95 gene, FAS can lead to the development of Autoimmune lymphoproliferative syndrome 1A (ALPS1A), an apoptotic disorder with early onset resulting in an accumulation of autoreactive lymphocytes (Peters et al. 1999).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Fusion protein comprising extracellular domain of human Fas linked to human Fc.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
Applications of CD95 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | 20ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD95 antibody
-
Mesdaghi, M. et al. (2010) Natural killer cells in allergic rhinitis patients and nonatopic controls.
Int Arch Allergy Immunol. 153 (3): 234-8. -
Ximeri, M. et al. (2010) Effect of lenalidomide therapy on hematopoiesis of patients with myelodysplastic syndrome associated with chromosome 5q deletion.
Haematologica. 95 (3): 406-14. -
Aref, S. et al. (2004) Accelerated neutrophil apoptosis in neutropenic patients with hepatosplenic schistosomiasis is induced by serum Fas ligand.
Hematol J. 5 (5): 434-9. -
Welsh, J.P. et al. (2004) In vitro effects of interferon-gamma and tumor necrosis factor-alpha on CD34+ bone marrow progenitor cells from aplastic anemia patients and normal donors.
Hematol J. 5 (1): 39-46. -
Wethkamp, N. et al. (2011) Daxx-beta and Daxx-gamma, two novel splice variants of the transcriptional co-repressor Daxx.
J Biol Chem. 286 (22): 19576-88. -
Chen, J.Y. et al. (2003) TNF-alpha renders human peritoneal mesothelial cells sensitive to anti-Fas antibody-induced apoptosis.
Nephrol Dial Transplant. 18 (9): 1741-7. -
Papadaki, H.A. et al. (2002) Bone marrow progenitor cell reserve and function and stromal cell function are defective in rheumatoid arthritis: evidence for a tumor necrosis factor alpha-mediated effect.
Blood. 99 (5): 1610-9. -
Mavroudi, I. et al. (2011) The CD40/CD40 ligand interactions exert pleiotropic effects on bone marrow granulopoiesis.
J Leukoc Biol. 89 (5): 771-83. -
Pyrovolaki, K. et al. (2009) Increased expression of CD40 on bone marrow CD34+ hematopoietic progenitor cells in patients with systemic lupus erythematosus: contribution to Fas-mediated apoptosis.
Arthritis Rheum. 60 (2): 543-52. -
Boula, A. et al. (2006) Effect of cA2 anti-tumor necrosis factor-alpha antibody therapy on hematopoiesis of patients with myelodysplastic syndromes.
Clin Cancer Res. 12 (10): 3099-108. -
Papadaki, H.A. et al. (2005) Normal bone marrow hematopoietic stem cell reserves and normal stromal cell function support the use of autologous stem cell transplantation in patients with multiple sclerosis.
Bone Marrow Transplant. 36 (12): 1053-63. -
Bachsais, M. et al. (2016) The Interaction of CD154 with the α5β1 Integrin Inhibits Fas-Induced T Cell Death.
PLoS One. 11 (7): e0158987. -
Ismail, M. et al. (2001) Bcl-2 and Bcl-x expression in the CD34+ cells of aplastic anaemia patients: relationship with increased apoptosis and upregulation of Fas antigen.
Br J Haematol. 113 (3): 706-12. -
Bachsais, M. et al. (2020) CD154 inhibits death of T cells via a Cis interaction with the α5β1 integrin.
PLoS One. 15 (8): e0235753. -
Ismail, M.M. et al. (2003) Differential apoptosis and Fas expression on GPI-negative and GPI-positive stem cells: a mechanism for the evolution of paroxysmal nocturnal haemoglobinuria.
Br J Haematol. 123 (3): 545-51.
Further Reading
-
Paulsen, M. & Janssen, O. (2011) Pro- and anti-apoptotic CD95 signaling in T cells.
Cell Commun Signal. 9: 7.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
