CD95 antibody | LOB 3/17



Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Fusion protein comprising extracellular domain of human Fas linked to human Fc.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
Applications of CD95 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | 1/50 | 1/100 | |
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Immunoprecipitation | 20ug/ml |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD95 antibody
-
Mesdaghi, M. et al. (2010) Natural killer cells in allergic rhinitis patients and nonatopic controls.
Int Arch Allergy Immunol. 153 (3): 234-8. -
Ximeri, M. et al. (2010) Effect of lenalidomide therapy on hematopoiesis of patients with myelodysplastic syndrome associated with chromosome 5q deletion.
Haematologica. 95 (3): 406-14. -
Aref, S. et al. (2004) Accelerated neutrophil apoptosis in neutropenic patients with hepatosplenic schistosomiasis is induced by serum Fas ligand.
Hematol J. 5 (5): 434-9. -
Welsh, J.P. et al. (2004) In vitro effects of interferon-gamma and tumor necrosis factor-alpha on CD34+ bone marrow progenitor cells from aplastic anemia patients and normal donors.
Hematol J. 5 (1): 39-46. -
Wethkamp, N. et al. (2011) Daxx-beta and Daxx-gamma, two novel splice variants of the transcriptional co-repressor Daxx.
J Biol Chem. 286 (22): 19576-88. -
Chen, J.Y. et al. (2003) TNF-alpha renders human peritoneal mesothelial cells sensitive to anti-Fas antibody-induced apoptosis.
Nephrol Dial Transplant. 18 (9): 1741-7. -
Papadaki, H.A. et al. (2002) Bone marrow progenitor cell reserve and function and stromal cell function are defective in rheumatoid arthritis: evidence for a tumor necrosis factor alpha-mediated effect.
Blood. 99 (5): 1610-9. -
Mavroudi, I. et al. (2011) The CD40/CD40 ligand interactions exert pleiotropic effects on bone marrow granulopoiesis.
J Leukoc Biol. 89 (5): 771-83. -
Pyrovolaki, K. et al. (2009) Increased expression of CD40 on bone marrow CD34+ hematopoietic progenitor cells in patients with systemic lupus erythematosus: contribution to Fas-mediated apoptosis.
Arthritis Rheum. 60 (2): 543-52. -
Boula, A. et al. (2006) Effect of cA2 anti-tumor necrosis factor-alpha antibody therapy on hematopoiesis of patients with myelodysplastic syndromes.
Clin Cancer Res. 12 (10): 3099-108. -
Papadaki, H.A. et al. (2005) Normal bone marrow hematopoietic stem cell reserves and normal stromal cell function support the use of autologous stem cell transplantation in patients with multiple sclerosis.
Bone Marrow Transplant. 36 (12): 1053-63. -
Bachsais, M. et al. (2016) The Interaction of CD154 with the α5β1 Integrin Inhibits Fas-Induced T Cell Death.
PLoS One. 11 (7): e0158987. -
Ismail, M. et al. (2001) Bcl-2 and Bcl-x expression in the CD34+ cells of aplastic anaemia patients: relationship with increased apoptosis and upregulation of Fas antigen.
Br J Haematol. 113 (3): 706-12. -
Bachsais, M. et al. (2020) CD154 inhibits death of T cells via a Cis interaction with the α5β1 integrin.
PLoS One. 15 (8): e0235753. -
Ismail, M.M. et al. (2003) Differential apoptosis and Fas expression on GPI-negative and GPI-positive stem cells: a mechanism for the evolution of paroxysmal nocturnal haemoglobinuria.
Br J Haematol. 123 (3): 545-51.
Further Reading
-
Paulsen, M. & Janssen, O. (2011) Pro- and anti-apoptotic CD95 signaling in T cells.
Cell Commun Signal. 9: 7.
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