CD95 antibody | LOB 3/17

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Mouse anti Human CD95:FITC

Mouse anti Human CD95

Product Type
Monoclonal Antibody
Clone
LOB 3/17
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1539FT F 25 µg
MCA1539F F 0.1 mg
MCA1539 F IP 0.2 mg
MCA1539T F IP 25 µg
Mouse anti Human CD95 antibody, clone LOB 3/17 recognizes the human CD95 cell surface antigen, also known as Tumor necrosis factor receptor superfamily member 6, Fas, Apo-1 antigen, Apoptosis-mediating surface antigen FAS or FASLG receptor. CD95 is a 310 amino acid ~40-50 kDa single pass type I transmembrane glycoprotein expressed by activated T and B cells, NK cells and thymocytes. Mutations in the CD95 gene, FAS can lead to the development of Autoimmune lymphoproliferative syndrome 1A (ALPS1A), an apoptotic disorder with early onset resulting in an accumulation of autoreactive lymphocytes (Peters et al. 1999).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Fusion protein comprising extracellular domain of human Fas linked to human Fc.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P25445 Related reagents
Entrez Gene
FAS Related reagents
Regulatory
For research purposes only

Applications of CD95 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation 20ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD95 antibody

  1. Mesdaghi, M. et al. (2010) Natural killer cells in allergic rhinitis patients and nonatopic controls.
    Int Arch Allergy Immunol. 153 (3): 234-8.
  2. Ximeri, M. et al. (2010) Effect of lenalidomide therapy on hematopoiesis of patients with myelodysplastic syndrome associated with chromosome 5q deletion.
    Haematologica. 95 (3): 406-14.
  3. Aref, S. et al. (2004) Accelerated neutrophil apoptosis in neutropenic patients with hepatosplenic schistosomiasis is induced by serum Fas ligand.
    Hematol J. 5 (5): 434-9.
  4. Welsh, J.P. et al. (2004) In vitro effects of interferon-gamma and tumor necrosis factor-alpha on CD34+ bone marrow progenitor cells from aplastic anemia patients and normal donors.
    Hematol J. 5 (1): 39-46.
  5. Wethkamp, N. et al. (2011) Daxx-beta and Daxx-gamma, two novel splice variants of the transcriptional co-repressor Daxx.
    J Biol Chem. 286 (22): 19576-88.
  6. Chen, J.Y. et al. (2003) TNF-alpha renders human peritoneal mesothelial cells sensitive to anti-Fas antibody-induced apoptosis.
    Nephrol Dial Transplant. 18 (9): 1741-7.
  7. Papadaki, H.A. et al. (2002) Bone marrow progenitor cell reserve and function and stromal cell function are defective in rheumatoid arthritis: evidence for a tumor necrosis factor alpha-mediated effect.
    Blood. 99 (5): 1610-9.
  8. Mavroudi, I. et al. (2011) The CD40/CD40 ligand interactions exert pleiotropic effects on bone marrow granulopoiesis.
    J Leukoc Biol. 89 (5): 771-83.
  9. Pyrovolaki, K. et al. (2009) Increased expression of CD40 on bone marrow CD34+ hematopoietic progenitor cells in patients with systemic lupus erythematosus: contribution to Fas-mediated apoptosis.
    Arthritis Rheum. 60 (2): 543-52.
  10. Boula, A. et al. (2006) Effect of cA2 anti-tumor necrosis factor-alpha antibody therapy on hematopoiesis of patients with myelodysplastic syndromes.
    Clin Cancer Res. 12 (10): 3099-108.
  11. Papadaki, H.A. et al. (2005) Normal bone marrow hematopoietic stem cell reserves and normal stromal cell function support the use of autologous stem cell transplantation in patients with multiple sclerosis.
    Bone Marrow Transplant. 36 (12): 1053-63.
  12. Bachsais, M. et al. (2016) The Interaction of CD154 with the α5β1 Integrin Inhibits Fas-Induced T Cell Death.
    PLoS One. 11 (7): e0158987.

Further Reading

  1. Paulsen, M. & Janssen, O. (2011) Pro- and anti-apoptotic CD95 signaling in T cells.
    Cell Commun Signal. 9: 7.