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CD95 antibody | LOB 3/17

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Anti Human CD95 Antibody, clone LOB 3/17 thumbnail image 1 Anti Human CD95 Antibody, clone LOB 3/17 thumbnail image 2 Anti Human CD95 Antibody, clone LOB 3/17 thumbnail image 3
Anti Human CD95 Antibody, clone LOB 3/17 gallery image 1
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD45 (MCA87A647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD45 (MCA87A647) and FITC conjugated Mouse anti Human CD95 (MCA1539F). All experiments performed on human blood gated on monocytes in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Human CD95 Antibody, clone LOB 3/17 gallery image 2
Figure A. Alexa Fluor647 conjugated Mouse anti Human CD45 (MCA87A647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor647 conjugated Mouse anti Human CD45 (MCA87A647) and FITC conjugated Mouse anti Human CD95 (MCA1539F). All experiments performed on human blood gated on lymphocytes in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Human CD95 Antibody, clone LOB 3/17 gallery image 3
Published customer image:
Mouse anti Human CD95 antibody, clone LOB 3/17 (MCA1539) used for the evaluation of CD95 expression on HUT78 T cell lymphoma cells by flow cytometry
Image caption:
Soluble CD154 inhibits Fas-mediated cell death in HUT78 T cells.
(A, Left and middle panels) HUT78 cells were incubated with anti-α5β1 JBS5 mAb, anti-CD40 G28.5 mAb, anti-Fas LOB3/17 mAb, or IgG isotype control (IC) followed by mouse anti-human Ig labeled with Alexa 488. (A, Right panel) Cells were incubated with Avidin-Alexa 488 (negative control) or sCD154-Alexa 488 (20ng/sample). (B, Left panel) HUT78 T cells were left non-stimulated (NS) or stimulated with different concentrations of anti-Fas CH-11 mAb or IgM isotype control (IC) for 48 hours at 37°C and assessed for their cell death response by FACS analysis as the percentage of death obtained by propidium iodide staining. (B, Right panel) HUT78 cells were pre-treated with different concentrations of sCD154, then left non-stimulated (NS) or stimulated with the anti-Fas CH-11 mAb (200ng/ml) for 48 h at 37°C. Cell death was then evaluated as above (± SD, * p <0.05, ** p <0.01, *** p <0.001 CH-11 versus IC).

From: Bachsais M, Naddaf N, Yacoub D, Salti S, Alaaeddine N, Aoudjit F, et al. (2016)
The Interaction of CD154 with the α5β1 Integrin Inhibits Fas-Induced T Cell Death.
PLoS ONE 11(7): e0158987.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.

Mouse anti Human CD95:FITC

Mouse anti Human CD95

Product Type
Monoclonal Antibody
Clone
LOB 3/17
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1539F
Datasheet
F
SDS
0.1 mg loader

MCA1539FT
Datasheet
F
SDS
25 µg loader

MCA1539T
Datasheet
F IP
SDS
25 µg loader

MCA1539
Datasheet
F IP
SDS
0.2 mg loader

Mouse anti Human CD95 antibody, clone LOB 3/17 recognizes the human CD95 cell surface antigen, also known as Tumor necrosis factor receptor superfamily member 6, Fas, Apo-1 antigen, Apoptosis-mediating surface antigen FAS or FASLG receptor. CD95 is a 310 amino acid ~40-50 kDa single pass type I transmembrane glycoprotein expressed by activated T and B cells, NK cells and thymocytes. Mutations in the CD95 gene, FAS can lead to the development of Autoimmune lymphoproliferative syndrome 1A (ALPS1A), an apoptotic disorder with early onset resulting in an accumulation of autoreactive lymphocytes (Peters et al. 1999).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Fusion protein comprising extracellular domain of human Fas linked to human Fc.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P25445
Entrez Gene
FAS
Regulatory
For research purposes only

Applications of CD95 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation 20ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Product Specific References

References for CD95 antibody

  1. Mesdaghi, M. et al. (2010) Natural killer cells in allergic rhinitis patients and nonatopic controls.
    Int Arch Allergy Immunol. 153 (3): 234-8.
  2. Ximeri, M. et al. (2010) Effect of lenalidomide therapy on hematopoiesis of patients with myelodysplastic syndrome associated with chromosome 5q deletion.
    Haematologica. 95 (3): 406-14.
  3. Aref, S. et al. (2004) Accelerated neutrophil apoptosis in neutropenic patients with hepatosplenic schistosomiasis is induced by serum Fas ligand.
    Hematol J. 5 (5): 434-9.
  4. Welsh, J.P. et al. (2004) In vitro effects of interferon-gamma and tumor necrosis factor-alpha on CD34+ bone marrow progenitor cells from aplastic anemia patients and normal donors.
    Hematol J. 5 (1): 39-46.
  5. Wethkamp, N. et al. (2011) Daxx-beta and Daxx-gamma, two novel splice variants of the transcriptional co-repressor Daxx.
    J Biol Chem. 286 (22): 19576-88.
  6. Chen, J.Y. et al. (2003) TNF-alpha renders human peritoneal mesothelial cells sensitive to anti-Fas antibody-induced apoptosis.
    Nephrol Dial Transplant. 18 (9): 1741-7.
  7. Papadaki, H.A. et al. (2002) Bone marrow progenitor cell reserve and function and stromal cell function are defective in rheumatoid arthritis: evidence for a tumor necrosis factor alpha-mediated effect.
    Blood. 99 (5): 1610-9.
  8. Mavroudi, I. et al. (2011) The CD40/CD40 ligand interactions exert pleiotropic effects on bone marrow granulopoiesis.
    J Leukoc Biol. 89 (5): 771-83.
  9. Pyrovolaki, K. et al. (2009) Increased expression of CD40 on bone marrow CD34+ hematopoietic progenitor cells in patients with systemic lupus erythematosus: contribution to Fas-mediated apoptosis.
    Arthritis Rheum. 60 (2): 543-52.
  10. Boula, A. et al. (2006) Effect of cA2 anti-tumor necrosis factor-alpha antibody therapy on hematopoiesis of patients with myelodysplastic syndromes.
    Clin Cancer Res. 12 (10): 3099-108.
  11. Papadaki, H.A. et al. (2005) Normal bone marrow hematopoietic stem cell reserves and normal stromal cell function support the use of autologous stem cell transplantation in patients with multiple sclerosis.
    Bone Marrow Transplant. 36 (12): 1053-63.
  12. Bachsais, M. et al. (2016) The Interaction of CD154 with the α5β1 Integrin Inhibits Fas-Induced T Cell Death.
    PLoS One. 11 (7): e0158987.
  13. Ismail, M. et al. (2001) Bcl-2 and Bcl-x expression in the CD34+ cells of aplastic anaemia patients: relationship with increased apoptosis and upregulation of Fas antigen.
    Br J Haematol. 113 (3): 706-12.
  14. Bachsais, M. et al. (2020) CD154 inhibits death of T cells via a Cis interaction with the α5β1 integrin.
    PLoS One. 15 (8): e0235753.
  15. Ismail, M.M. et al. (2003) Differential apoptosis and Fas expression on GPI-negative and GPI-positive stem cells: a mechanism for the evolution of paroxysmal nocturnal haemoglobinuria.
    Br J Haematol. 123 (3): 545-51.

Further Reading

  1. Paulsen, M. & Janssen, O. (2011) Pro- and anti-apoptotic CD95 signaling in T cells.
    Cell Commun Signal. 9: 7.

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