CD90 antibody | F15-42-1
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|Mouse anti Human CD90 antibody, clone F15-42-1 recognizes the human CD90 cell surface antigen, a ~25 kDa glycoprotein homologous to rat Thy1. The antigen is expressed by a subset of CD34+ve cells in the bone marrow and by prothymocytes within the thymus. CD90 is also expressed extensively within the brain.
Mouse anti Human CD90 antibody, clone F15-42-1 is routinely tested in flow cytometry on the MOLT4 cell line.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Cynomolgus monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- MCA90: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA90T: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Purified human brain Thy-1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1|
|Immunohistology - Paraffin|
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Human Brain, thymus
References for CD90 antibody
Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Exp Anim. 49 (2): 97-110.
McKenzie, J.L. & Fabre, J.W. (1981) Human thy-1: unusual localization and possible functional significance in lymphoid tissues.
J Immunol. 126 (3): 843-50.
Daar, A.S. & Fabre, J.W. (1981) Demonstration with monoclonal antibodies of an unusual mononuclear cell infiltrate and loss of normal epithelial membrane antigens in human breast carcinomas.
Lancet. 2 (8244): 434-8.
Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577.
Fiegel, H.C. et al. (2004) Stem-like cells in human hepatoblastoma.
J Histochem Cytochem. 52 (11): 1495-501.
Hagood, J.S. et al. (2005) Loss of fibroblast Thy-1 expression correlates with lung fibrogenesis.
Am J Pathol. 167 (2): 365-79.
Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
J Cell Physiol. 214: 75-83.
Cox, G. et al. (2011) The use of the reamer-irrigator-aspirator to harvest mesenchymal stem cells.
J Bone Joint Surg Br. 93: 517-24.
View The Latest Product References
Cizeau, J. et al. (2011) Fusogenics: a recombinant immunotoxin-based screening platform to select internalizing tumor-specific antibody fragments.
J Biomol Screen. 16: 90-100.
Gieseke, F. et al. (2010) Human multipotent mesenchymal stromal cells use galectin-1 to inhibit immune effector cells.
Blood. 116: 3770-9.
Hauser, P.V. et al. (2010) Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.
Am J Pathol. 177: 2011-21.
Holzwarth, C. et al. (2010) Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells.
BMC Cell Biol. 11:11
Karlsen, T.A. et al. (2010) Human primary articular chondrocytes, chondroblasts-like cells, and dedifferentiated chondrocytes: differences in gene, microRNA, and protein expression and phenotype.
Tissue Eng Part C Methods. 17: 219-27.
Manochantr, S. et al. (2010) Isolation, characterization and neural differentiation potential of amnion derived mesenchymal stem cells.
J Med Assoc Thai. 93 Suppl 7: S183-91.
Meng, J. et al (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
PLoS One. 6: e17454.
Pessina, A. et al. (2010) CD45+/CD133+ positive cells expanded from umbilical cord blood expressing PDX-1 and markers of pluripotency.
Cell Biol Int. 34: 783-90.
Tome, M. et al. (2007) Calponin is expressed by subpopulations of connective tissue cells but not olfactory ensheathing cells in the neonatal olfactory mucosa.
BMC Neurosci. 8: 74.
Yin, S. et al. (2010) Chondrogenic transdifferentiation of human dermal fibroblasts stimulated with cartilage-derived morphogenetic protein 1.
Tissue Eng Part A. 16: 1633-43.
Shafaei, H. et al. (2011) Effects of human placental serum on proliferation and morphology of human adipose tissue-derived stem cells.
Bone Marrow Transplant. 46: 1464-71.
Escobar, C.H. & Chaparro, O. (2016) Xeno-Free Extraction, Culture, and Cryopreservation of Human Adipose-Derived Mesenchymal Stem Cells.
Stem Cells Transl Med. 5 (3): 358-65.
Shinoda, K. et al. (2016) Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche for memory T-helper cells in allergic airway inflammation.
Proc Natl Acad Sci U S A. May 2. pii: 201512600. [Epub ahead of print]
Kamprom, W. et al. (2016) Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells.
Stem Cells Int. 2016: 2514326.
Vaquero, J. et al. (2016) An approach to personalized cell therapy in chronic complete paraplegia: The Puerta de Hierro phase I/II clinical trial.
Cytotherapy. 18 (8): 1025-36.
Zhang, X. et al. (2017) Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells.
PLoS One. 12 (6): e0180138.
GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
PLoS One. 13 (2): e0192244.
Chaturvedi, C.P. et al. (2018) Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.
Stem Cells Int. 2018: 6901761.
Noda, S. et al. (2019) Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.
Sci Rep. 9 (1): 5430.
Sanjurjo-Rodriguez, C. et al. (2020) Gene Expression Signatures of Synovial Fluid Multipotent Stromal Cells in Advanced Knee Osteoarthritis and Following Knee Joint Distraction.
Front Bioeng Biotechnol. 8: 579751.
Supokawej, A. et al. (2013) Cardiogenic and myogenic gene expression in mesenchymal stem cells after 5-azacytidine treatment.
Turk J Haematol. 30 (2): 115-21.
Paiboon, N. et al. (2019) Gestational Tissue-Derived Human Mesenchymal Stem Cells Use Distinct Combinations of Bioactive Molecules to Suppress the Proliferation of Human Hepatoblastoma and Colorectal Cancer Cells.
Stem Cells Int. 2019: 9748795.
Song, H. et al. (2019) MIF/CD74 axis participates in inflammatory activation of Schwann cells following sciatic nerve injury.
J Mol Histol. 50 (4): 355-67.
Fujii-Tezuka, R. et al. (2021) Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential.
Regen Ther. 16: 1-11.
Orikasa, S. et al. (2022) Hypoxia-inducible factor 1α induces osteo/odontoblast differentiation of human dental pulp stem cells via Wnt/β-catenin transcriptional cofactor BCL9.
Sci Rep. 12 (1): 682.
- Entrez Gene
- GO Terms
- GO:0001525 angiogenesis
- GO:0007010 cytoskeleton organization
- GO:0005783 endoplasmic reticulum
- GO:0005100 Rho GTPase activator activity
- GO:0005887 integral to plasma membrane
- GO:0005178 integrin binding
- GO:0006469 negative regulation of protein kinase activity
- GO:0016337 cell-cell adhesion
- GO:0030336 negative regulation of cell migration
- View More GO Terms
- GO:0030426 growth cone
- GO:0034235 GPI anchor binding
- GO:0043547 positive regulation of GTPase activity
- GO:0045121 membrane raft
- GO:0046549 retinal cone cell development
- GO:0048041 focal adhesion assembly
- GO:0050771 negative regulation of axonogenesis
- GO:0050852 T cell receptor signaling pathway
- GO:0050860 negative regulation of T cell receptor signaling pathway
- GO:0050870 positive regulation of T cell activation
- GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
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