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CD90 antibody | F15-42-1

Product Code Applications Pack Size List Price Your Price Qty
MCA90T
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SDS Safety Datasheet SDS
C * F IF IP 20 µg loader
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loader
MCA90
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SDS Safety Datasheet SDS
C * F IF IP 0.2 mg loader
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loader

Mouse anti Human CD90 antibody, clone F15-42-1 recognizes the human CD90 cell surface antigen, a ~25 kDa glycoprotein homologous to rat Thy1. The antigen is expressed by a subset of CD34+ve cells in the bone marrow and by prothymocytes within the thymus. CD90 is also expressed extensively within the brain.

Mouse anti Human CD90 antibody, clone F15-42-1 is routinely tested in flow cytometry on the MOLT4 cell line.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA90T: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
MCA90: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Purified human brain Thy-1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Human Brain, thymus

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Source Reference

  1. McKenzie, J.L. & Fabre, J.W. (1981) Human thy-1: unusual localization and possible functional significance in lymphoid tissues.
    J Immunol. 126 (3): 843-50.

References for CD90 antibody

  1. Daar, A.S. & Fabre, J.W. (1981) Demonstration with monoclonal antibodies of an unusual mononuclear cell infiltrate and loss of normal epithelial membrane antigens in human breast carcinomas.
    Lancet. 2 (8244): 434-8.
  2. Fiegel, H.C. et al. (2004) Stem-like cells in human hepatoblastoma.
    J Histochem Cytochem. 52 (11): 1495-501.
  3. Hagood, J.S. et al. (2005) Loss of fibroblast Thy-1 expression correlates with lung fibrogenesis.
    Am J Pathol. 167 (2): 365-79.
  4. Tome, M. et al. (2007) Calponin is expressed by subpopulations of connective tissue cells but not olfactory ensheathing cells in the neonatal olfactory mucosa.
    BMC Neurosci. 8: 74.
  5. Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
    J Cell Physiol. 214: 75-83.
  6. Pessina, A. et al. (2010) CD45+/CD133+ positive cells expanded from umbilical cord blood expressing PDX-1 and markers of pluripotency.
    Cell Biol Int. 34: 783-90.
  7. Manochantr, S. et al. (2010) Isolation, characterization and neural differentiation potential of amnion derived mesenchymal stem cells.
    J Med Assoc Thai. 93 Suppl 7: S183-91.
  8. Karlsen, T.A. et al. (2010) Human primary articular chondrocytes, chondroblasts-like cells, and dedifferentiated chondrocytes: differences in gene, microRNA, and protein expression and phenotype.
    Tissue Eng Part C Methods. 17: 219-27.
  9. View The Latest Product References
  10. Hauser, P.V. et al. (2010) Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.
    Am J Pathol. 177: 2011-21.
  11. Yin, S. et al. (2010) Chondrogenic transdifferentiation of human dermal fibroblasts stimulated with cartilage-derived morphogenetic protein 1.
    Tissue Eng Part A. 16: 1633-43.
  12. Gieseke, F. et al. (2010) Human multipotent mesenchymal stromal cells use galectin-1 to inhibit immune effector cells.
    Blood. 116: 3770-9.
  13. Holzwarth, C. et al. (2010) Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells.
    BMC Cell Biol. 11:11
  14. Meng, J. et al (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
    PLoS One. 6: e17454.
  15. Cizeau, J. et al. (2011) Fusogenics: a recombinant immunotoxin-based screening platform to select internalizing tumor-specific antibody fragments.
    J Biomol Screen. 16: 90-100.
  16. Cox, G. et al. (2011) The use of the reamer-irrigator-aspirator to harvest mesenchymal stem cells.
    J Bone Joint Surg Br. 93: 517-24.
  17. Shafaei, H. et al. (2011) Effects of human placental serum on proliferation and morphology of human adipose tissue-derived stem cells.
    Bone Marrow Transplant. 46: 1464-71.
  18. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
    PLoS One. 7: e35577.
  19. Supokawej, A. et al. (2013) Cardiogenic and myogenic gene expression in mesenchymal stem cells after 5-azacytidine treatment.
    Turk J Haematol. 30 (2): 115-21.
  20. Escobar, C.H. & Chaparro, O. (2016) Xeno-Free Extraction, Culture, and Cryopreservation of Human Adipose-Derived Mesenchymal Stem Cells.
    Stem Cells Transl Med. 5 (3): 358-65.
  21. Shinoda, K. et al. (2016) Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche for memory T-helper cells in allergic airway inflammation.
    Proc Natl Acad Sci U S A. 113 (20): E2842-51.
  22. Kamprom, W. et al. (2016) Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells.
    Stem Cells Int. 2016: 2514326.
  23. Vaquero, J. et al. (2016) An approach to personalized cell therapy in chronic complete paraplegia: The Puerta de Hierro phase I/II clinical trial.
    Cytotherapy. 18 (8): 1025-36.
  24. Zhang, X. et al. (2017) Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells.
    PLoS One. 12 (6): e0180138.
  25. Garikipati, V. N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
    PLoS One. 13 (2): e0192244.
  26. Chaturvedi, C.P. et al. (2018) Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.
    Stem Cells Int. 2018: 6901761.
  27. Noda, S. et al. (2019) Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.
    Sci Rep. 9 (1): 5430.
  28. Song, H. et al. (2019) MIF/CD74 axis participates in inflammatory activation of Schwann cells following sciatic nerve injury.
    J Mol Histol. 50 (4): 355-67.
  29. Paiboon, N. et al. (2019) Gestational Tissue-Derived Human Mesenchymal Stem Cells Use Distinct Combinations of Bioactive Molecules to Suppress the Proliferation of Human Hepatoblastoma and Colorectal Cancer Cells.
    Stem Cells Int. 2019: 9748795.
  30. Sanjurjo-Rodriguez, C. et al. (2020) Gene Expression Signatures of Synovial Fluid Multipotent Stromal Cells in Advanced Knee Osteoarthritis and Following Knee Joint Distraction.
    Front Bioeng Biotechnol. 8: 579751.
  31. Fujii-Tezuka, R. et al. (2021) Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential.
    Regen Ther. 16: 1-11.
  32. Orikasa, S. et al. (2022) Hypoxia-inducible factor 1α induces osteo/odontoblast differentiation of human dental pulp stem cells via Wnt/β-catenin transcriptional cofactor BCL9.
    Sci Rep. 12 (1): 682.
  33. Sirithammajak, S. et al. (2022) Human Mesenchymal Stem Cells Derived from the Placenta and Chorion Suppress the Proliferation while Enhancing the Migration of Human Breast Cancer Cells.
    Stem Cells Int. 2022: 4020845.
  34. Arenal, Á. et al. (2022) Effects of Cardiac Stem Cell on Postinfarction Arrhythmogenic Substrate.
    Int J Mol Sci. 23 (24): 16211.
  35. Kruchen, A. et al. (2023) Epigenetic Modification of Mesenchymal Stromal Cells Derived from Bone Marrow and Embryonal Tumors to Facilitate Immunotherapeutic Approaches in Pediatric Malignancies.
    Curr Issues Mol Biol. 45 (3): 2121-35.
  36. Payet, M. et al. (2023) Inflammatory Mesenchymal Stem Cells Express Abundant Membrane-Bound and Soluble Forms of C-Type Lectin-like CD248.
    Int J Mol Sci. 24 (11): 9546.
  37. Tiraihi, T. et al. (2023) A Sequential Culturing System for Generating Epithelial-Like Stem Cells from Human Mesenchymal Stem Cells Derived from Adipose Tissue
    Cell Tissue Biol. 17 (6): 639-52.
  38. Tripathy, N.K. et al. (2018) Cardiomyogenic Heterogeneity of Clonal Subpopulations of Human Bone Marrow Mesenchymal Stem Cells.
    J Stem Cells Regen Med. 14 (1): 27-33.

Synonyms
THY1
RRID
AB_871984
UniProt
P04216
Entrez Gene
THY1
GO Terms
GO:0001525 angiogenesis
GO:0007010 cytoskeleton organization
GO:0005783 endoplasmic reticulum
GO:0005100 Rho GTPase activator activity
GO:0005887 integral to plasma membrane
GO:0005178 integrin binding
GO:0006469 negative regulation of protein kinase activity
GO:0016337 cell-cell adhesion
GO:0030336 negative regulation of cell migration
GO:0030426 growth cone
GO:0034235 GPI anchor binding
GO:0043547 positive regulation of GTPase activity
GO:0045121 membrane raft
GO:0046549 retinal cone cell development
GO:0048041 focal adhesion assembly
GO:0050771 negative regulation of axonogenesis
GO:0050852 T cell receptor signaling pathway
GO:0050860 negative regulation of T cell receptor signaling pathway
GO:0050870 positive regulation of T cell activation
GO:0051281 positive regulation of release of sequestered calcium ion into cytosol

MCA90T

150022 159864

MCA90

162167 1711

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