CD90 antibody | F15-42-1

Mouse anti Human CD90

Product Type
Monoclonal Antibody
Clone
F15-42-1
Isotype
IgG1
Specificity
CD90

Product Code Applications Pack Size List Price Your Price Qty
MCA90
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF IP 0.2 mg loader
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loader
MCA90T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF IP 20 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD90 antibody, clone F15-42-1 recognizes the human CD90 cell surface antigen, a ~25 kDa glycoprotein homologous to rat Thy1. The antigen is expressed by a subset of CD34+ve cells in the bone marrow and by prothymocytes within the thymus. CD90 is also expressed extensively within the brain.

Mouse anti Human CD90 antibody, clone F15-42-1 is routinely tested in flow cytometry on the MOLT4 cell line.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA90: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA90T: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Purified human brain Thy-1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Human Brain, thymus

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References for CD90 antibody

  1. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  2. McKenzie, J.L. & Fabre, J.W. (1981) Human thy-1: unusual localization and possible functional significance in lymphoid tissues.
    J Immunol. 126 (3): 843-50.
  3. Daar, A.S. & Fabre, J.W. (1981) Demonstration with monoclonal antibodies of an unusual mononuclear cell infiltrate and loss of normal epithelial membrane antigens in human breast carcinomas.
    Lancet. 2 (8244): 434-8.
  4. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
    PLoS One. 7: e35577.
  5. Fiegel, H.C. et al. (2004) Stem-like cells in human hepatoblastoma.
    J Histochem Cytochem. 52 (11): 1495-501.
  6. Hagood, J.S. et al. (2005) Loss of fibroblast Thy-1 expression correlates with lung fibrogenesis.
    Am J Pathol. 167 (2): 365-79.
  7. Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
    J Cell Physiol. 214: 75-83.
  8. Cox, G. et al. (2011) The use of the reamer-irrigator-aspirator to harvest mesenchymal stem cells.
    J Bone Joint Surg Br. 93: 517-24.
  9. View The Latest Product References
  10. Cizeau, J. et al. (2011) Fusogenics: a recombinant immunotoxin-based screening platform to select internalizing tumor-specific antibody fragments.
    J Biomol Screen. 16: 90-100.
  11. Gieseke, F. et al. (2010) Human multipotent mesenchymal stromal cells use galectin-1 to inhibit immune effector cells.
    Blood. 116: 3770-9.
  12. Hauser, P.V. et al. (2010) Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.
    Am J Pathol. 177: 2011-21.
  13. Holzwarth, C. et al. (2010) Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells.
    BMC Cell Biol. 11:11
  14. Karlsen, T.A. et al. (2010) Human primary articular chondrocytes, chondroblasts-like cells, and dedifferentiated chondrocytes: differences in gene, microRNA, and protein expression and phenotype.
    Tissue Eng Part C Methods. 17: 219-27.
  15. Manochantr, S. et al. (2010) Isolation, characterization and neural differentiation potential of amnion derived mesenchymal stem cells.
    J Med Assoc Thai. 93 Suppl 7: S183-91.
  16. Meng, J. et al (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
    PLoS One. 6: e17454.
  17. Pessina, A. et al. (2010) CD45+/CD133+ positive cells expanded from umbilical cord blood expressing PDX-1 and markers of pluripotency.
    Cell Biol Int. 34: 783-90.
  18. Tome, M. et al. (2007) Calponin is expressed by subpopulations of connective tissue cells but not olfactory ensheathing cells in the neonatal olfactory mucosa.
    BMC Neurosci. 8: 74.
  19. Yin, S. et al. (2010) Chondrogenic transdifferentiation of human dermal fibroblasts stimulated with cartilage-derived morphogenetic protein 1.
    Tissue Eng Part A. 16: 1633-43.
  20. Shafaei, H. et al. (2011) Effects of human placental serum on proliferation and morphology of human adipose tissue-derived stem cells.
    Bone Marrow Transplant. 46: 1464-71.
  21. Escobar, C.H. & Chaparro, O. (2016) Xeno-Free Extraction, Culture, and Cryopreservation of Human Adipose-Derived Mesenchymal Stem Cells.
    Stem Cells Transl Med. 5 (3): 358-65.
  22. Shinoda, K. et al. (2016) Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche for memory T-helper cells in allergic airway inflammation.
    Proc Natl Acad Sci U S A. May 2. pii: 201512600. [Epub ahead of print]
  23. Kamprom, W. et al. (2016) Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells.
    Stem Cells Int. 2016: 2514326.
  24. Vaquero, J. et al. (2016) An approach to personalized cell therapy in chronic complete paraplegia: The Puerta de Hierro phase I/II clinical trial.
    Cytotherapy. 18 (8): 1025-36.
  25. Zhang, X. et al. (2017) Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells.
    PLoS One. 12 (6): e0180138.
  26. GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
    PLoS One. 13 (2): e0192244.
  27. Chaturvedi, C.P. et al. (2018) Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.
    Stem Cells Int. 2018: 6901761.
  28. Noda, S. et al. (2019) Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.
    Sci Rep. 9 (1): 5430.
  29. Sanjurjo-Rodriguez, C. et al. (2020) Gene Expression Signatures of Synovial Fluid Multipotent Stromal Cells in Advanced Knee Osteoarthritis and Following Knee Joint Distraction.
    Front Bioeng Biotechnol. 8: 579751.
  30. Supokawej, A. et al. (2013) Cardiogenic and myogenic gene expression in mesenchymal stem cells after 5-azacytidine treatment.
    Turk J Haematol. 30 (2): 115-21.
  31. Paiboon, N. et al. (2019) Gestational Tissue-Derived Human Mesenchymal Stem Cells Use Distinct Combinations of Bioactive Molecules to Suppress the Proliferation of Human Hepatoblastoma and Colorectal Cancer Cells.
    Stem Cells Int. 2019: 9748795.
  32. Song, H. et al. (2019) MIF/CD74 axis participates in inflammatory activation of Schwann cells following sciatic nerve injury.
    J Mol Histol. 50 (4): 355-67.
  33. Fujii-Tezuka, R. et al. (2021) Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential.
    Regen Ther. 16: 1-11.
  34. Orikasa, S. et al. (2022) Hypoxia-inducible factor 1α induces osteo/odontoblast differentiation of human dental pulp stem cells via Wnt/β-catenin transcriptional cofactor BCL9.
    Sci Rep. 12 (1): 682.

Flow Cytometry

Immunofluorescence

Immunohistology - Paraffin

Synonyms
THY1
RRID
AB_871984
UniProt
P04216
Entrez Gene
THY1
GO Terms
GO:0001525 angiogenesis
GO:0007010 cytoskeleton organization
GO:0005783 endoplasmic reticulum
GO:0005100 Rho GTPase activator activity
GO:0005887 integral to plasma membrane
GO:0005178 integrin binding
GO:0006469 negative regulation of protein kinase activity
GO:0016337 cell-cell adhesion
GO:0030336 negative regulation of cell migration
GO:0030426 growth cone
GO:0034235 GPI anchor binding
GO:0043547 positive regulation of GTPase activity
GO:0045121 membrane raft
GO:0046549 retinal cone cell development
GO:0048041 focal adhesion assembly
GO:0050771 negative regulation of axonogenesis
GO:0050852 T cell receptor signaling pathway
GO:0050860 negative regulation of T cell receptor signaling pathway
GO:0050870 positive regulation of T cell activation
GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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