CD86 antibody | BU63

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MCA1118
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SDS Safety Datasheet SDS
C F IP 0.2 mg loader
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loader
MCA1118T
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C F IP 25 µg loader
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Mouse anti Human CD86 antibody, clone Bu63 recognizes human CD86 also known as B7-2, a type I transmembrane protein expressed by monocytes and activated B cells (Engel et al. 1994). CD86 acts as a co-stimulaory molecule along with CD80 (Lanier et al. 1995) and is a ligand for CD28 and CTLA-4 (Azuma et al. 1993).

CD86 is a member of the Immunoglobulin superfamily and carries an extracellular domain bearing both an Ig-v-like domain which contains the CTLA-4 binding site and an adjacent C2-like domain. CD86 plays an important role in co-stimulation of T cell proliferation (Freeman et al. 1993), IL-2 production (Ribot et al. 2012) and in the primary immune response (Schultze et al. 1996).

Domain depletion epitope mapping indicates that the binding site of Mouse anti Human CD86,clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeanin et al. 1997).

CD86 along with CD80 may be exploited as receptors for adenovirus entry into cells (Short et al. 2004 2006).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.2 mg
0.09% Sodium Azide (NaN3)
Pack Size: 25 µg
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human peripheral blood lymphocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse P3.X63 Ag8653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20 1/100
Immunohistology - Frozen 1/20 1/100
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Human Tonsil

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References for CD86 antibody

  1. McLellan, A.D. et al. (1999) Induction of dendritic cell costimulator molecule expression is suppressed by T cells in the absence of antigen-specific signalling: role of cluster formation, CD40 and HLA-class II for dendritic cell activation.
    Immunology. 98 (2): 171-80.
  2. Nozawa, Y. et al. (1993) A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells.
    J Pathol. 169 (3): 309-15.
  3. Goodyear, O. et al. (2010) Induction of a CD8+ T-cell response to the MAGE cancer testis antigen by combined treatment with azacitidine and sodium valproate in patients with acute myeloid leukemia and myelodysplasia.
    Blood. 116: 1908-18.
  4. Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
    J Immunol. 176 (10): 5730-4.
  5. Salte, T. et al. (2010) Increased intracellular growth of Mycobacterium avium in HIV-1 exposed monocyte-derived dendritic cells.
    Microbes Infect. 13: 276-83.
  6. Adler, H.S. et al. (2010) Neuronal nitric oxide synthase modulates maturation of human dendritic cells.
    J Immunol. 184: 6025-34.
  7. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
    BMC Immunol. 12:2.
  8. Kapsogeorgou, E.K. et al. (2001) Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor, but has reduced binding to CTLA4.
    J Immunol. 166: 3107-13.
  9. View The Latest Product References
  10. Lozanoska-Ochser, B. et al. (2008) Expression of CD86 on human islet endothelial cells facilitates T cell adhesion and migration.
    J Immunol. 181: 6109-16.
  11. Urban, B.C. et al. (2001) A role for CD36 in the regulation of dendritic cell function.
    Proc Natl Acad Sci U S A. 98: 8750-5.
  12. Zhan, H. et al. (2003) The immunomodulatory role of human conjunctival epithelial cells.
    Invest Ophthalmol Vis Sci. 44: 3906-10.
  13. Sprater, F. et al. (2012) Expression of ESE-3 isoforms in immunogenic and tolerogenic human monocyte-derived dendritic cells.
    PLoS One. 7 (11): e49577.
  14. McCarthy, N.E. et al. (2013) Proinflammatory Vδ2+ T Cells Populate the Human Intestinal Mucosa and Enhance IFN-γ Production by Colonic αβ T Cells.
    J Immunol. 191: 2752-63.
  15. Hofmann-Wellenhof, R. et al. (2004) Sunburn cell formation, dendritic cell migration, and immunomodulatory factor production after solar-simulated irradiation of sunscreen-treated human skin explants in vitro.
    J Invest Dermatol. 123: 781-7.
  16. Rajkovic, I. et al. (2011) Differences in T-helper polarizing capability between human monocyte-derived dendritic cells and monocyte-derived Langerhans'-like cells.
    Immunology. 132: 217-25.
  17. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
    J Biomed Biotechnol. 2012: 172420.
  18. Ikezumi, Y. et al. (2021) Steroid treatment promotes an M2 anti-inflammatory macrophage phenotype in childhood lupus nephritis.
    Pediatr Nephrol. 36 (2): 349-59.

Flow Cytometry

Immunofluorescence

Synonyms
B7-2
RRID
AB_321774
UniProt
P42081
Entrez Gene
CD86
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0007267 cell-cell signaling
GO:0008284 positive regulation of cell proliferation
GO:0015026 coreceptor activity
GO:0016563 transcription activator activity
GO:0031295 T cell costimulation
GO:0043017 positive regulation of lymphotoxin A biosynthetic process
GO:0044419 interspecies interaction between organisms
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045404 positive regulation of interleukin-4 biosynthetic process
GO:0045630 positive regulation of T-helper 2 cell differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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