CD86 antibody | BU63
FITC conjugated Mouse anti Human CD86 antibody, clone Bu63 (MCA1118F) used for phenotyping monocyte derived dendritic cells by flow cytometry.
Image caption:
Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry.
Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1α,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-α, IL-1β, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.
From: Sprater F, Hovden A-O, Appel S (2012)
Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.
PLoS ONE 7(11): e49577.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD86 antibody, clone Bu63 (MCA1118) used for the evaluation of CD86 expression on cell populations by flow cytometry.
Image caption:
DC differentiation from H1 hESC.
Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.
From: Silk KM, Leishman AJ, Nishimoto KP, Reddy A, Fairchild PJ.
Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012;2012:172420.
doi: 10.1155/2012/172420.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F ResetMouse anti Human CD86
- Product Type
- Monoclonal Antibody
- Clone
- BU63
- Isotype
- IgG1
- Specificity
- CD86
Antibody Quality and Performance Guarantee
| Mouse anti Human CD86 antibody, clone Bu63 recognizes human CD86 also known as B7-2, a type I transmembrane protein expressed by monocytes and activated B cells (Engel et al. 1994). CD86 acts as a co-stimulaory molecule along with CD80 (Lanier et al. 1995) and is a ligand for CD28 and CTLA-4 (Azuma et al. 1993). CD86 is a member of the Immunoglobulin superfamily and carries an extracellular domain bearing both an Ig-v-like domain which contains the CTLA-4 binding site and an adjacent C2-like domain. CD86 plays an important role in co-stimulation of T cell proliferation (Freeman et al. 1993), IL-2 production (Ribot et al. 2012) and in the primary immune response (Schultze et al. 1996). Domain depletion epitope mapping indicates that the binding site of Mouse anti Human CD86,clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeanin et al. 1997). CD86 along with CD80 may be exploited as receptors for adenovirus entry into cells (Short et al. 2004 2006). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human peripheral blood lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse P3.X63 Ag8653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/20 | 1/100 |
| Immunohistology - Frozen | |
1/20 | 1/100 |
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl.
- Histology Positive Control Tissue
- Human Tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD86 antibody
-
McLellan, A.D. et al. (1999) Induction of dendritic cell costimulator molecule expression is suppressed by T cells in the absence of antigen-specific signalling: role of cluster formation, CD40 and HLA-class II for dendritic cell activation.
Immunology. 98 (2): 171-80. -
Nozawa, Y. et al. (1993) A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells.
J Pathol. 169 (3): 309-15. -
Goodyear, O. et al. (2010) Induction of a CD8+ T-cell response to the MAGE cancer testis antigen by combined treatment with azacitidine and sodium valproate in patients with acute myeloid leukemia and myelodysplasia.
Blood. 116: 1908-18. -
Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
J Immunol. 176 (10): 5730-4. -
Salte, T. et al. (2010) Increased intracellular growth of Mycobacterium avium in HIV-1 exposed monocyte-derived dendritic cells.
Microbes Infect. 13: 276-83. -
Adler, H.S. et al. (2010) Neuronal nitric oxide synthase modulates maturation of human dendritic cells.
J Immunol. 184: 6025-34. -
Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
BMC Immunol. 12:2. -
Kapsogeorgou, E.K. et al. (2001) Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor, but has reduced binding to CTLA4.
J Immunol. 166: 3107-13.
View The Latest Product References
-
Lozanoska-Ochser, B. et al. (2008) Expression of CD86 on human islet endothelial cells facilitates T cell adhesion and migration.
J Immunol. 181: 6109-16. -
Urban, B.C. et al. (2001) A role for CD36 in the regulation of dendritic cell function.
Proc Natl Acad Sci U S A. 98: 8750-5. -
Zhan, H. et al. (2003) The immunomodulatory role of human conjunctival epithelial cells.
Invest Ophthalmol Vis Sci. 44: 3906-10. -
Sprater, F. et al. (2012) Expression of ESE-3 isoforms in immunogenic and tolerogenic human monocyte-derived dendritic cells.
PLoS One. 7 (11): e49577. -
McCarthy, N.E. et al. (2013) Proinflammatory Vδ2+ T Cells Populate the Human Intestinal Mucosa and Enhance IFN-γ Production by Colonic αβ T Cells.
J Immunol. 191: 2752-63. -
Hofmann-Wellenhof, R. et al. (2004) Sunburn cell formation, dendritic cell migration, and immunomodulatory factor production after solar-simulated irradiation of sunscreen-treated human skin explants in vitro.
J Invest Dermatol. 123: 781-7. -
Rajkovic, I. et al. (2011) Differences in T-helper polarizing capability between human monocyte-derived dendritic cells and monocyte-derived Langerhans'-like cells.
Immunology. 132: 217-25. -
Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012: 172420. -
Ikezumi, Y. et al. (2021) Steroid treatment promotes an M2 anti-inflammatory macrophage phenotype in childhood lupus nephritis.
Pediatr Nephrol. 36 (2): 349-59.
- Synonyms
- B7-2
- RRID
- AB_321774
- UniProt
- P42081
- Entrez Gene
- CD86
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0007267 cell-cell signaling
- GO:0008284 positive regulation of cell proliferation
- GO:0015026 coreceptor activity
- GO:0016563 transcription activator activity
- GO:0031295 T cell costimulation
- GO:0043017 positive regulation of lymphotoxin A biosynthetic process
- GO:0044419 interspecies interaction between organisms
- View More GO Terms
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045404 positive regulation of interleukin-4 biosynthetic process
- GO:0045630 positive regulation of T-helper 2 cell differentiation
MCA1118
150552
1711
MCA1118T
150467
161587
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