Mouse anti Human CD82 antibody, clone B-L2 recognizes human CD82, also known as C33 antigen, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein or Tetraspanin-27. CD82 is a 267 amino acid ~50-53 kDa multiple pass transmembrane glycoprotein and member of the tetraspanin family expressed by T cells, NK cells, monocytes, granulocytes and platelets.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- CD82 transfected murine mastocytoma cell line P815.
- Fusion Partners
- Spleen cells from immunized BALB/c (Iffa Credo) mice were fused with cells of the mouse X63/Ag.8653 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to plasma membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD82 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD82 antibody
Ono, M. et al. (1999) Motility inhibition and apoptosis are induced by metastasis-suppressing gene product CD82 and its analogue CD9, with concurrent glycosylation.
Cancer Res. 59 (10): 2335-9.
Mazurov, D. et al. (2006) HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membrane.
Virology. 346 (1): 194-204.
Todeschini AR et al. (2007) Ganglioside GM2-tetraspanin CD82 complex inhibits met and its cross-talk with integrins, providing a basis for control of cell motility through glycosynapse.
J Biol Chem. 282 (11): 8123-33.
Mazurov, D. et al. (2007) The inner loop of tetraspanins CD82 and CD81 mediates interactions with human T cell lymphotrophic virus type 1 Gag protein.
J Biol Chem. 282 (6): 3896-903.
Wang, H. et al. (2012) N-Glycosylation pattern of recombinant human CD82 (KAI1), a tumor-associated membrane protein.
J Proteomics. 75 (4): 1375-85.
Zuidscherwoude M et al. (2015) The tetraspanin web revisited by super-resolution microscopy.
Sci Rep. 5: 12201.
Jones, E.L. et al. (2016) Dendritic Cell Migration and Antigen Presentation Are Coordinated by the Opposing Functions of the Tetraspanins CD82 and CD37.
J Immunol. 196 (3): 978-87.
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