CD82 antibody | B-L2
Filter by Application:IF Reset
Mouse anti Human CD82
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD82 antibody, clone B-L2 recognizes human CD82, also known as C33 antigen, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein or Tetraspanin-27. CD82 is a 267 amino acid ~50-53 kDa multiple pass transmembrane glycoprotein and member of the tetraspanin family expressed by T cells, NK cells, monocytes, granulocytes and platelets.|
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
- CD82 transfected murine mastocytoma cell line P815.
- Fusion Partners
- Spleen cells from immunized BALB/c (Iffa Credo) mice were fused with cells of the mouse X63/Ag.8653 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD82 antibody
Ono, M. et al. (1999) Motility inhibition and apoptosis are induced by metastasis-suppressing gene product CD82 and its analogue CD9, with concurrent glycosylation.
Cancer Res. 59 (10): 2335-9.
Mazurov, D. et al. (2006) HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membrane.
Virology. 346 (1): 194-204.
Todeschini AR et al. (2007) Ganglioside GM2-tetraspanin CD82 complex inhibits met and its cross-talk with integrins, providing a basis for control of cell motility through glycosynapse.
J Biol Chem. 282 (11): 8123-33.
Mazurov, D. et al. (2007) The inner loop of tetraspanins CD82 and CD81 mediates interactions with human T cell lymphotrophic virus type 1 Gag protein.
J Biol Chem. 282 (6): 3896-903.
Wang, H. et al. (2012) N-Glycosylation pattern of recombinant human CD82 (KAI1), a tumor-associated membrane protein.
J Proteomics. 75 (4): 1375-85.
Zuidscherwoude M et al. (2015) The tetraspanin web revisited by super-resolution microscopy.
Sci Rep. 5: 12201.
Jones, E.L. et al. (2016) Dendritic Cell Migration and Antigen Presentation Are Coordinated by the Opposing Functions of the Tetraspanins CD82 and CD37.
J Immunol. 196 (3): 978-87.
Santos, R.C.M. et al. (2022) GM2/GM3 controls the organizational status of CD82/Met microdomains: further studies in GM2/GM3 complexation.
Glycoconj J. 39 (5): 653-61.
View The Latest Product References
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.Yes, sign me up