CD82 antibody | B-L2

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Mouse anti Human CD82

Product Type
Monoclonal Antibody
Clone
B-L2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1311 C F IF 2 ml
Mouse anti Human CD82 antibody, clone B-L2 recognizes human CD82, also known as C33 antigen, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein or Tetraspanin-27. CD82 is a 267 amino acid ~50-53 kDa multiple pass transmembrane glycoprotein and member of the tetraspanin family expressed by T cells, NK cells, monocytes, granulocytes and platelets.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
CD82 transfected murine mastocytoma cell line P815.
Fusion Partners
Spleen cells from immunized BALB/c (Iffa Credo) mice were fused with cells of the mouse X63/Ag.8653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P27701 Related reagents
Entrez Gene
CD82 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005887 integral to plasma membrane
Regulatory
For research purposes only

Applications of CD82 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for CD82 antibody

  1. Ono, M. et al. (1999) Motility inhibition and apoptosis are induced by metastasis-suppressing gene product CD82 and its analogue CD9, with concurrent glycosylation.
    Cancer Res. 59 (10): 2335-9.
  2. Mazurov, D. et al. (2006) HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membrane.
    Virology. 346 (1): 194-204.
  3. Todeschini AR et al. (2007) Ganglioside GM2-tetraspanin CD82 complex inhibits met and its cross-talk with integrins, providing a basis for control of cell motility through glycosynapse.
    J Biol Chem. 282 (11): 8123-33.
  4. Mazurov, D. et al. (2007) The inner loop of tetraspanins CD82 and CD81 mediates interactions with human T cell lymphotrophic virus type 1 Gag protein.
    J Biol Chem. 282 (6): 3896-903.
  5. Wang, H. et al. (2012) N-Glycosylation pattern of recombinant human CD82 (KAI1), a tumor-associated membrane protein.
    J Proteomics. 75 (4): 1375-85.
  6. Zuidscherwoude M et al. (2015) The tetraspanin web revisited by super-resolution microscopy.
    Sci Rep. 5: 12201.
  7. Jones, E.L. et al. (2016) Dendritic Cell Migration and Antigen Presentation Are Coordinated by the Opposing Functions of the Tetraspanins CD82 and CD37.
    J Immunol. 196 (3): 978-87.