CD8 Beta antibody | 2ST8.5H7

CD8 molecules are composed of dimers of either alpha/alpha or alpha/beta polypeptide combinations. Both types of dimer may co-exist at the cell surface. Clone 2ST8.5H7 is also referred to as clone T8/2T8-5H7 and binding is dependent on the expression of the alpha/beta dimer.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.2ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Preparation
- Purified IgG prepared by affinity chromatography from Ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 1% Bovine Serum Albumin
- Immunogen
- Normal human T lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
Applications of CD8 Beta antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Immunoprecipitation | |||
Western Blotting |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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Product Specific References
References for CD8 Beta antibody
-
Terry, L.A. et al. (1990) Differential expression and regulation of the human CD8 alpha and CD8 beta chains.
Tissue Antigens 35: 82-91. -
DiSanto, J.P. et al. (1991) Generation of anti-human CD8 beta-specific antibodies using transfectants expressing mixed-species CD8 heterodimers.
J Immunol Methods. 141 (1): 123-31. -
de la Calle-Martin, O. et al. (2001) Familial CD8 deficiency due to a mutation in the CD8 alpha gene.
J Clin Invest. 108: 117-23. -
Schmitz, J.E. et al (1998) Expression of the CD8alpha beta-heterodimer on CD8(+) T lymphocytes in peripheral blood lymphocytes of human immunodeficiency virus- and human immunodeficiency virus+ individuals.
Blood. 92: 198-206.
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