Mouse anti Human CD8 beta antibody, clone 2ST8.5H7 recognizes the human CD8 cell surface antigen. The antibody binds to the beta chain, recognizing an epitope that is dependent upon expression of both CD8 alpha and CD8 beta.
CD8 molecules are composed of dimers of either alpha/alpha or alpha/beta polypeptide combinations. Both types of dimer may co-exist at the cell surface. Clone 2ST8.5H7 is also referred to as clone T8/2T8-5H7 and binding is dependent on the expression of the alpha/beta dimer.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilized
- Reconstitute with 0.2ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
- Purified IgG prepared by affinity chromatography from Ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 1% Bovine Serum Albumin
- Normal human T lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD8 Beta antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD8 Beta antibody
Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Exp Anim. 49 (2): 97-110.
Fry, T.J. et al. (2002) IL-7 therapy dramatically alters peripheral T-cell homeostasis in normal and SIV-infected nonhuman primates.
Blood. 101: 2294-9.
Terry, L.A. et al. (1990) Differential expression and regulation of the human CD8 alpha and CD8 beta chains.
Tissue Antigens 35: 82-91.
DiSanto, J.P. et al. (1991) Generation of anti-human CD8 beta-specific antibodies using transfectants expressing mixed-species CD8 heterodimers.
J Immunol Methods. 141 (1): 123-31.
de la Calle-Martin, O. et al. (2001) Familial CD8 deficiency due to a mutation in the CD8 alpha gene.
J Clin Invest. 108: 117-23.
Schmitz, J.E. et al (1998) Expression of the CD8alpha beta-heterodimer on CD8(+) T lymphocytes in peripheral blood lymphocytes of human immunodeficiency virus- and human immunodeficiency virus+ individuals.
Blood. 92: 198-206.
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