CD8 antibody | LT8
Mouse anti Human CD8 antibody, clone LT8 (MCA1226GA) used for the evaluation of CD8α exptession on T lymphocytes from marmoset blood, spleen and lung by flow cytometry.
Image caption:
The percentage of the total leucocyte count for various cell types identified in naive marmosets (a) cells in naïve blood, (b) in naive spleens, and (c) in naive lungs. CD8 T cells are expressed as a percentage of CD3+ cells. Bar represents the median value.
From: M. Nelson and M. Loveday,
“Exploring the Innate Immunological Response of an Alternative Nonhuman Primate Model of Infectious Disease; the Common Marmoset,”
Journal of Immunology Research, vol. 2014, Article ID 913632, 8 pages, 2014.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Alexafluor®700 conjugated Mouse anti Human CD8 antibody, clone LT8 used for the evaluation of CD8 expression on PBMC by flow cytometry.
Image caption:
Gating stategy for cell surface marker analysis in uncultured and cultured PBMC. Gates were applied in the numerical order shown.
From: Manivannan K, Rowan AG, Tanaka Y, Taylor GP, Bangham CRM (2016)
CADM1/TSLC1 Identifies HTLV-1-Infected Cells and Determines Their Susceptibility to CTL-Mediated Lysis.
PLoS Pathog 12(4): e1005560.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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F C FLA ResetMouse anti Human CD8
- Product Type
- Monoclonal Antibody
- Clone
- LT8
- Isotype
- IgG1
- Specificity
- CD8
Quick Links:
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| Mouse anti Human CD8 antibody, clone LT8 recognizes the human CD8 cell surface glycoprotein expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells. The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Marmoset Chimpanzee Cynomolgus monkey Red-bellied Tamarin - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Normal human peripheral blood lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or cells or 100μl human whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD8 antibody
-
Zarkesh-Esfahani, H. et al. (2001) High-dose leptin activates human leukocytes via receptor expression on monocytes.
J Immunol. 167 (8): 4593-9. -
Manninen, A. & Saksela, K. (2002) HIV-1 Nef interacts with inositol trisphosphate receptor to activate calcium signaling in T cells.
J Exp Med. 195 (8): 1023-32. -
Parnes, J.R. (1989) Molecular biology and function of CD4 and CD8.
Adv Immunol. 44: 265-311. -
Kap, Y.S. et al. (2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
J Histochem Cytochem. 57: 1159-67. -
Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
BMC Immunol. 12: 2. -
Nelson, M. et al. (2010) Characterization of lethal inhalational infection with Francisella tularensis in the common marmoset (Callithrix jacchus).
J Med Microbiol. 59: 1107-13. -
Gibbings, D.J. et al. (2007) CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses.
BMC Immunol. 8: 12. -
Junker, A. et al. (2007) Multiple sclerosis: T-cell receptor expression in distinct brain regions.
Brain. 130: 2789-99.
View The Latest Product References
-
Held, K. et al. (2011) Expression of herpes simplex virus 1-encoded microRNAs in human trigeminal ganglia and their relation to local T-cell infiltrates.
J Virol. 85 (19): 9680-5. -
Hood SP et al. (2014) Changes in immune cell populations in the periphery and liver of GBV-B-infected and convalescent tamarins (Saguinus labiatus).
Virus Res. 179: 93-101. -
Nelson, M. & Loveday, M. (2014) Exploring the innate immunological response of an alternative nonhuman primate model of infectious disease; the common marmoset.
J Immunol Res. 2014: 913632. -
Manivannan, K. et al. (2016) CADM1/TSLC1 Identifies HTLV-1-Infected Cells and Determines Their Susceptibility to CTL-Mediated Lysis.
PLoS Pathog. 12 (4): e1005560. -
Gross, C.C. et al. (2016) Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation.
Proc Natl Acad Sci U S A. 113 (21): E2973-82. -
Dunham, J. et al. (2016) Blockade of CD127 Exerts a Dichotomous Clinical Effect in Marmoset Experimental Autoimmune Encephalomyelitis.
J Neuroimmune Pharmacol. 11 (1): 73-83. -
Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
PLoS One. 12 (7): e0180088. -
Philippens, I.H. et al. (2017) Acceleration of Amyloidosis by Inflammation in the Amyloid-Beta Marmoset Monkey Model of Alzheimer's Disease.
J Alzheimers Dis. 55 (1): 101-113.
- RRID
- AB_321400
- UniProt
- P01732
- P10966
- Entrez Gene
- CD8A
- CD8B
- GO Terms
- GO:0005576 extracellular region
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005887 integral to plasma membrane
- GO:0015026 coreceptor activity
- GO:0019882 antigen processing and presentation
- GO:0042101 T cell receptor complex
- GO:0042288 MHC class I protein binding
- GO:0050776 regulation of immune response
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