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CD69 antibody | FN50

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Anti Human CD69 Antibody, clone FN50 thumbnail image 1 Anti Human CD69 Antibody, clone FN50 thumbnail image 2 Anti Human CD69 Antibody, clone FN50 thumbnail image 3 Anti Human CD69 Antibody, clone FN50 thumbnail image 4 Anti Human CD69 Antibody, clone FN50 thumbnail image 5 Anti Human CD69 Antibody, clone FN50 thumbnail image 6 Anti Human CD69 Antibody, clone FN50 thumbnail image 7 Anti Human CD69 Antibody, clone FN50 thumbnail image 8 Anti Human CD69 Antibody, clone FN50 thumbnail image 9 Anti Human CD69 Antibody, clone FN50 thumbnail image 10 Anti Human CD69 Antibody, clone FN50 thumbnail image 11 Anti Human CD69 Antibody, clone FN50 thumbnail image 12 Anti Human CD69 Antibody, clone FN50 thumbnail image 13 Anti Human CD69 Antibody, clone FN50 thumbnail image 14 Anti Human CD69 Antibody, clone FN50 thumbnail image 15 Anti Human CD69 Antibody, clone FN50 thumbnail image 16 Anti Human CD69 Antibody, clone FN50 thumbnail image 17 Anti Human CD69 Antibody, clone FN50 thumbnail image 18 Anti Human CD69 Antibody, clone FN50 thumbnail image 19 Anti Human CD69 Antibody, clone FN50 thumbnail image 20 Anti Human CD69 Antibody, clone FN50 thumbnail image 21 Anti Human CD69 Antibody, clone FN50 thumbnail image 22 Anti Human CD69 Antibody, clone FN50 thumbnail image 23 Anti Human CD69 Antibody, clone FN50 thumbnail image 24 Anti Human CD69 Antibody, clone FN50 thumbnail image 25 Anti Human CD69 Antibody, clone FN50 thumbnail image 26
Anti Human CD69 Antibody, clone FN50 gallery image 1
Figure A. Alexa Fluor488 conjugated mouse anti human CD3 (MCA463A488) and Alexa Fluor647 conjugated mouse IgG1 isotype control (MCA928A488). Figure B. Alexa Fluor488 conjugated mouse anti human CD3 (MCA463A488) and Alexa Fluor647 conjugated mouse anti human CD69 (MCA2806A647). All experiments performed on PBMCs extracted from human blood stimulated with PHA and LPS for 48 hours gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 2
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 515 conjugated Mouse anti Human CD69 (MCA2806SBV515). All experiments performed on PBMCs extracted from human blood stimulated with PHA and LPS for 48 hours, gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 3
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Violet 440 conjugated Mouse anti Human CD69 (MCA2806SBV440) All experiments performed on human blood stimulated with Cell Stimulation Reagent (without Brefeldin A) for 5 hours, gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 4
Figure A. Alexa Fluor 647 conjugated Mouse anti human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti human CD3 (MCA463A647) and StarBright Violet 610 conjugated Mouse anti Human CD69 (MCA2806SBV610). All experiments performed on red blood lysed human blood stimulated with Cell stimulation reagent for 5 hours. Gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 5
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 670 conjugated Mouse anti Human CD69 (MCA2806SBV670). All experiments performed on red cell lysed human blood stimulated with cell stimulation reagent for 6 hours gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 6
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Violet 570 conjugated Mouse anti Human CD69 (MCA2806SBV570). All experiments performed on red cell lysed human blood stimulated for 5 hours with cell stimulation reagent gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 7
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 710 conjugated Mouse anti Human CD69 (MCA2806SBV710). All experiments performed on red cell lysed human blood stimulated for 5 hours with cell stimulation reagent (BUF076) gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 8
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 790 conjugated Mouse anti Human CD69 (MCA2806SBV790). All experiments performed on PBMCs extracted from human blood stimulated with PHA and LPS for 48 hours, gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 9
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 475 conjugated Mouse anti Human CD69 (MCA2806SBV475). All experiments performed on human PBMCs stimulated with PHA and LPS for 48 hours gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 10
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright UltraViolet 400 conjugated Mouse anti Human CD69 (MCA2806SBUV400). All experiments performed on human PBMCs stimulated with PHA for 2 days, gated on live cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 11
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright Violet 760 conjugated Mouse anti Human CD69 (MCA2806SBV760). All experiments performed on PBMCs stimulated with PHA and LPS for 2 days gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 12
Figure A. StarBright Violet 790 conjugated mouse anti Human CD3 (MCA463SBV790). Figure B. StarBright Violet 790 conjugated mouse anti Human CD3 (MCA463SBV790) and StarBright UltraViolet 665 conjugated Mouse anti Human CD69 (MCA2806SBUV665). All experiments performed on PBMCs extracted from human blood stimulated with PHA and LPS for 48 hours. Gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 13
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790) and StarBright UltraViolet 445 conjugated Mouse anti Human CD69 (MCA2806SBUV445). All experiments performed on PBMCs extracted from human blood stimulated with PHA and LPS for 48 hours, gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 14
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright UltraViolet 795 conjugated Mouse anti Human CD69 (MCA2806SBUV795). All experiments performed on PBMCs extracted from human blood stimulated with PHA and LPS for 48 hours, gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 15
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790) and StarBright UltraViolet 510 conjugated Mouse anti Human CD69 (MCA2806SBUV510). All experiments performed on PBMCs from human blood stimulated with PHA and LPS for 48 hours, gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 16
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright Blue 615 conjugated Mouse anti Human CD69 (MCA2806SBB615). All experiments performed on red cell lysed human blood stimulated for 5 hours with PMA and ionomycin gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 17
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright Blue 675 conjugated Mouse anti Human CD69 (MCA2806SBB675). All experiments performed on red cell lysed human blood stimulated for 5 hours with PMA and ionomycin gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 18
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright Blue 765 conjugated Mouse anti Human CD69 (MCA2806SBB765). All experiments performed on red cell lysed human blood stimulated for 5 hours with PMA and ionomycin gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 19
Figure A. StarBright Yellow 665 conjugated Mouse anti Human CD3 (MCA463SBY665). Figure B. StarBright Yellow 665 conjugated Mouse anti Human CD3 (MCA463SBY665) and StarBright Blue 810 conjugated Mouse anti Human CD69 (MCA2806SBB810). All experiments performed on red cell lysed human blood stimulated for 5 hours with cell stimulation reagent gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 20
Figure A. StarBright Blue 765 conjugated Mouse anti Human CD3 (MCA463SBB765). Figure B. StarBright Blue 765 conjugated Mouse anti Human CD3 (MCA463SBB765) and StarBright Yellow 575 conjugated Mouse anti Human CD69 (MCA2806SBY575). All experiments performed on red cell lysed Human blood gated on live single cell lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 21
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610) and StarBright Yellow 720 conjugated Mouse anti Human CD69 (MCA2806SBY720). All experiments performed on red cell lysed human blood stimulated with cell stimulation reagent for 5 hours. Gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 22
Figure A. StarBright Blue 765 conjugated Mouse anti Human CD3 (MCA463SBB765). Figure B. StarBright Blue 765 conjugated Mouse anti Human CD3 (MCA463SBB765) and StarBright Yellow 605 conjugated Mouse anti Human CD69 (MCA2806SBY605). All experiments performed on red blood lysed human blood stimulated with Cell stimulation reagent for 5 hours gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 23
Figure A. StarBright Blue 765 conjugated Mouse anti Human CD3 (MCA463SBB765). Figure B. StarBright Blue 765 conjugated Mouse anti Human CD3 (MCA463SBB765) and StarBright Yellow 605 conjugated Mouse anti Human CD69 (MCA2806SBY605). All experiments performed on red blood lysed human blood stimulated with Cell stimulation reagent for 5 hours gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 24
Figure A. StarBright Ultra Violet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright Ultra Violet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright Yellow 800 conjugated Mouse anti Human CD69 (MCA2806SBY800). All experiments performed on red blood lysed human blood stimulated with Cell stimulation reagent for 5 hours. Gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 25
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright Blue 580 conjugated Mouse anti Human CD69 (MCA2806SBB580). All experiments performed on red blood lysed human blood stimulated with Cell stimulation reagent for 5 hours. Gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD69 Antibody, clone FN50 gallery image 26
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright Red 775 conjugated Mouse anti Human CD69 (MCA2806SBR775). All experiments performed on human blood stimulated with Cell Stimulation Reagent (without Brefeldin A) for 5 hours, gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD69

Product Type
Monoclonal Antibody
Clone
FN50
Isotype
IgG1
Specificity
CD69

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Product Code Applications Pack Size List Price Your Price Qty
MCA2806GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CD69 antibody, clone FN50 recognizes the human early activation antigen CD69, also known as activation inducer molecule (AIM), Early T-cell activation antigen p60, EA1 or MLR-3. CD69 is a 199 amino acid single pass type II transmembrane glycoprotein of ~30 kDa containing a single C-type lectin domain and a single potential N-glycosylation site. CD69 is expressed as a disulphide bond linked homodimer of ~60 kDa (López-Cabrera et al. 1993).

CD69 is a marker of early activation expressed by B and T lymphocytes, natural killer cells(Werfel 1997), neutrophils, thymocytes and platelets (Gaviol et al. 1992). Expression of CD69 is rapidly induced on activation by infection or chronic inflamation (Sancho et al. 2005). Multiple dimeric glycoforms of CD69 can be formed through differential glycosylation of the monomeric subunits (Vance et al. 1997).

Mouse anti Human CD69 , clone FN50 is useful for the detection of CD69 by flow cytometry and immunohistochemistry on frozen tissue sections.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Baboon
Chimpanzee
Cynomolgus monkey
Rhesus Monkey
Macaque
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Activated human B-cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (Fc):FITC
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

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Description Mouse IgG1 Negative Control

References for CD69 antibody

  1. Holte, H. et al. (1989) Ki67 and 4F2 antigen expression as well as DNA synthesis predict survival at relapse/tumour progression in low-grade B-cell lymphoma.
    Int J Cancer. 44 (6): 975-80.
  2. Herberth, M. et al. (2010) Differential effects on T-cell function following exposure to serum from schizophrenia smokers.
    Mol Psychiatry. 15 (4): 364-71.
  3. Schaeuble, K. et al. (2011) Cross-talk between TCR and CCR7 signaling sets a temporal threshold for enhanced T lymphocyte migration.
    J Immunol. 187 (11): 5645-52.
  4. Sela, M. et al. (2011) Sequential phosphorylation of SLP-76 at tyrosine 173 is required for activation of T and mast cells.
    EMBO J. 30 (15): 3160-72.
  5. Garbe, Y. et al. (2011) Semiallogenic fusions of MSI(+) tumor cells and activated B cells induce MSI-specific T cell responses.
    BMC Cancer. 11: 410.
  6. Schwitalle, Y. et al. (2004) Immunogenic peptides generated by frameshift mutations in DNA mismatch repair-deficient cancer cells.
    Cancer Immun. 4: 14.
  7. Sutavani, R.V. et al. (2013) CD55 Costimulation Induces Differentiation of a Discrete T Regulatory Type 1 Cell Population with a Stable Phenotype.
    J Immunol. 191: 5895-903.
  8. Walter, G.J. et al. (2013) Interaction with activated monocytes enhances cytokine expression and suppressive activity of human CD4+CD45ro+CD25+CD127(low) regulatory T cells.
    Arthritis Rheum. 65: 627-38.
    9. View The Latest Product References
  9. Kuric, E. et al. (2017) Demonstration of Tissue Resident Memory CD8 T Cells in Insulitic Lesions in Adult Patients with Recent-Onset Type 1 Diabetes.
    Am J Pathol. 187 (3): 581-8.
  10. Karnell, F.G. et al. (2017) Reconstitution of immune cell populations in multiple sclerosis patients after autologous stem cell transplantation.
    Clin Exp Immunol. 189 (3): 268-278.
  11. Rossatti, P. et al. (2022) Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.
    BMC Biol. 20 (1): 189.

Flow Cytometry

Synonyms
AIM
RRID
AB_11152603
UniProt
Q07108
Entrez Gene
CD69
GO Terms
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding

MCA2806GA

0913 149337 166330

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