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CD66e antibody | C365D3(NCRC23)

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Anti Human CD66e Antibody, clone C365D3(NCRC23) gallery image 1
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Mouse anti Human CD66e antibody, clone C365D3 (MCA1744) used for the evaluation of CD66e exppression on PaCa 5061 cells by flow cytometry.
Image caption:
Immunostaining and cell surface expression of epithelial/pancreatic markers and overexpressed proteins in PaCa 5061 cells.
FACS profiles of PaCa 5061 cells. Cell surface expression of CD44, EpCAM, EGFR, CEACAM1, CEACAM5 and CEACAM6 were obtained with specific antibodies as in materials and methods. Each histogram shows cell surface expression of the corresponding marker (filled curves) and the irrelevant, isotype-matched antibody (open curves).

From: Kalinina T, Güngör C, Thieltges S, Möller-Krull M, Penas EM, Wicklein D, Streichert T, Schumacher U, Kalinin V, Simon R, Otto B, Dierlamm J, Schwarzenbach H, Effenberger KE, Bockhorn M, Izbicki JR, Yekebas EF.
Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung.
BMC Cancer. 2010 Jun 16;10:295.

Mouse anti Human CD66e:FITC

Mouse anti Human CD66e

Product Type
Monoclonal Antibody
Clone
C365D3(NCRC23)
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1744FT F 25 µg
MCA1744F F 0.1 mg
MCA1744T C E F P* 25 µg
MCA1744 C E F P* 0.2 mg
Mouse anti Human CD66e antibody, clone C365D3 (NCRC23) recognizes human Carcinoembryonic antigen-related cell adhesion molecule 5, also known as CD66e, carcinoembryonic antigen, Meconium antigen 100, CEA or CEACAM5. CD66e is a 702 amino acid ~77 kDa GPI anchored membrane protein containing 7 Ig-like domains. Mouse anti Human CD66e antibody, clone C365D3 does not cross-react with normal cross-reacting antigen (CD66c), or with biliary g lycoprotein (CD66a) as indicated by binding assays (Price 1988, note: in this study Mouse anti Human CD66e antibody, clone C365D3 is designated as clone 6 (from author)).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3NSI myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

This product is photosensitive and should be protected from the light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P06731 Related reagents
Entrez Gene
CEACAM5 Related reagents
GO Terms
GO:0005887 integral to plasma membrane
GO:0031225 anchored to membrane
GO:0016323 basolateral plasma membrane
Regulatory
For research purposes only

Applications of CD66e antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
ELISA
Flow Cytometry
Immunohistology - Frozen 1/1000
Immunohistology - Paraffin 1 1/1000
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD66e antibody

  1. Seth, J. et al. (1988) Carcinoembryonic antigen.
    Lancet. 1 (8599): 1399.
  2. Soucek, K. et al. (2010) Fetal colon cell line FHC exhibits tumorigenic phenotype, complex karyotype, and TP53 gene mutation.
    Cancer Genet Cytogenet. 197: 107-16.
  3. Kalinina, T. et al. (2010) Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung.
    BMC Cancer.10: 295.
  4. Dallas, M.R. et al. (2012) Divergent roles of CD44 and carcinoembryonic antigen in colon cancer metastasis.
    FASEB J. 226: 2648-56.
  5. Stern-Ginossar. N. et al. (2007) Intercellular Transfer of Carcinoembryonic Antigen from Tumor Cells to NK Cells.
    J Immunol. 2007 Oct 1;1 79: 4424-34.
  6. Ferro, F. et al. (2011) Adipose tissue-derived stem cell in vitro differentiation in a three-dimensional dental bud structure.
    Am J Pathol.178: 2299-310.
  7. Chao, A. et al. (2006) Molecular characterization of adenocarcinoma and squamous carcinoma of the uterine cervix using microarray analysis of gene expression.
    Int J Cancer. 119: 91-8.
  8. Domenis, R. et al. (2015) Adipose tissue derived stem cells: in vitro and in vivo analysis of a standard and three commercially available cell-assisted lipotransfer techniques.
    Stem Cell Res Ther. 6: 2.
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