CD66e antibody | C365D3 (NCRC23)

Mouse anti Human CD66e

Product Type
Monoclonal Antibody
Clone
C365D3 (NCRC23)
Isotype
IgG1
Specificity
CD66e

Product Code Applications Pack Size List Price Your Price Qty
MCA1744
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F P * 0.2 mg loader
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loader
MCA1744T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F P * 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD66e antibody, clone C365D3 (NCRC23) recognizes human Carcinoembryonic antigen-related cell adhesion molecule 5, also known as CD66e, carcinoembryonic antigen, Meconium antigen 100, CEA or CEACAM5. CD66e is a 702 amino acid ~77 kDa GPI anchored membrane protein containing 7 Ig-like domains. Mouse anti Human CD66e antibody, clone C365D3 does not cross-react with normal cross-reacting antigen (CD66c), or with biliary glycoprotein 1 (CD66a) as indicated by binding assays (Price 1988, note: in this study Mouse anti Human CD66e antibody, clone C365D3 is designated as clone 6 (from author)).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA1744: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
MCA1744T: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3NSI myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen 1/1000
Immunohistology - Paraffin 1 1/1000
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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loader
Description Mouse IgG1 Negative Control

References for CD66e antibody

  1. Seth, J. et al. (1988) Carcinoembryonic antigen.
    Lancet. 1 (8599): 1399.
  2. Soucek, K. et al. (2010) Fetal colon cell line FHC exhibits tumorigenic phenotype, complex karyotype, and TP53 gene mutation.
    Cancer Genet Cytogenet. 197: 107-16.
  3. Kalinina, T. et al. (2010) Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung.
    BMC Cancer.10: 295.
  4. Dallas, M.R. et al. (2012) Divergent roles of CD44 and carcinoembryonic antigen in colon cancer metastasis.
    FASEB J. 226: 2648-56.
  5. Stern-Ginossar. N. et al. (2007) Intercellular Transfer of Carcinoembryonic Antigen from Tumor Cells to NK Cells.
    J Immunol. 2007 Oct 1;1 79: 4424-34.
  6. Ferro, F. et al. (2011) Adipose tissue-derived stem cell in vitro differentiation in a three-dimensional dental bud structure.
    Am J Pathol.178: 2299-310.
  7. Chao, A. et al. (2006) Molecular characterization of adenocarcinoma and squamous carcinoma of the uterine cervix using microarray analysis of gene expression.
    Int J Cancer. 119: 91-8.
  8. Domenis, R. et al. (2015) Adipose tissue derived stem cells: in vitro and in vivo analysis of a standard and three commercially available cell-assisted lipotransfer techniques.
    Stem Cell Res Ther. 6: 2.
  9. View The Latest Product References
  10. Wicklein, D. et al. (2018) CEACAM1 promotes melanoma metastasis and is involved in the regulation of the EMT associated gene network in melanoma cells.
    Sci Rep. 8 (1): 11893.
  11. Caponnetto, F. et al. (2020) Human Adipose-Derived Stem Cells in Madelung's Disease: Morphological and Functional Characterization.
    Cells: 10 (1): 44.

Flow Cytometry

Synonyms
CEA
RRID
AB_322726
UniProt
P06731
Entrez Gene
CEACAM5
GO Terms
GO:0005887 integral to plasma membrane
GO:0031225 anchored to membrane
GO:0016323 basolateral plasma membrane
View more products with CD66 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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