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CD66b antibody | 80H3

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Anti Human CD66b Antibody, clone 80H3 thumbnail image 1 Anti Human CD66b Antibody, clone 80H3 thumbnail image 2 Anti Human CD66b Antibody, clone 80H3 thumbnail image 3 Anti Human CD66b Antibody, clone 80H3 thumbnail image 4
Anti Human CD66b Antibody, clone 80H3 gallery image 1
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD16 (MCA5665A647) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 FITC (STAR132). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD16 (MCA5665A647) and purified Mouse anti Human CD66b (MCA216) detected with Goat anti Mouse IgG1 FITC (STAR132). All experiments performed on human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD66b Antibody, clone 80H3 gallery image 2
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD24 and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B . Alexa Fluor 647 conjugated Mouse anti Human CD24 and FITC conjugated Mouse anti Human CD66b (MCA216F). All experiments performed on human peripheral blood gated on live single granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD66b Antibody, clone 80H3 gallery image 3
Figure A. APC conjugated mouse anti human CD14 (MCA596APC) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. APC conjugated mouse anti human CD14 (MCA596APC) and FITC conjugated mouse anti human CD66b (MCA216F). All experiments performed on red cell lysed Human peripheral blood gated on myeloid cells in the presence of Human SeroBlock (BUF070A).
Anti Human CD66b Antibody, clone 80H3 gallery image 4
Figure A. PE conjugated Mouse anti Human CD24 (MCA1379PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated Mouse anti Human CD24 (MCA1379PE) and FITC conjugated Mouse anti Human CD66b (MCA216F). All experiments performed on human blood gated on single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Human CD66b:FITC

Product Type
Monoclonal Antibody
Clone
80H3
Isotype
IgG1
Specificity
CD66b

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Product Code Applications Pack Size List Price Your Price Qty
MCA216F
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SDS Safety Datasheet SDS
F 100 Tests/2ml loader
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Mouse anti Human CD66b antibody, clone 80H3 reacts with the CEACAM8 or CGM6 gene product, from the carcinoembryonic gene family. The CEACAM8 gene product is called CD66b, Non-specific cross-reacting antigen NCA-95 or Carcinoembryonic antigen CGM6. CD66b is a 286 amino acid mature ~95-100 kDa GPI anchored molecule with an N-terminal 34 amino acid signal peptide and a 29 amino acid C-terminal propeptide, shed in the mature form. CD66b is expressed strongly by mature granulocytes and metamyelocytes, and weakly by bone marrow myelocytes. CD66b is not expressed by peripheral blood monocytes or lymphocytes.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
Human leucocytes from individuals with CML.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse MOPC 315-43 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/2
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 20ul of the suggested working dilution to label 5 x 105 cells or 100ul whole blood.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD66b antibody

  1. Mannoni, P. et al. (1982) Monoclonal antibodies against human granulocytes and myeloid differentiation antigens.
    Hum Immunol. 5 (4): 309-23.
  2. Ionita, M.G. et al. (2010) High neutrophil numbers in human carotid atherosclerotic plaques are associated with characteristics of rupture-prone lesions.
    Arterioscler Thromb Vasc Biol. 30 (9): 1842-8.
  3. Simard, J.C. et al. (2010) Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism.
    J Leukoc Biol. 87 (5): 905-14.
  4. Feuk-Lagerstedt, E. et al. (1999) Identification of CD66a and CD66b as the major galectin-3 receptor candidates in human neutrophils.
    J Immunol. 163: 5592-8.
  5. Jankowski, A. et al. (2002) Determinants of the phagosomal pH in neutrophils.
    J Biol Chem. 277: 6059-66.
  6. Jinnouchi, A. et al. (2005) Local anesthetics inhibit priming of neutrophils by lipopolysaccharide for enhanced release of superoxide: suppression of cytochrome b558 expression by disparate mechanisms.
    J Leukoc Biol. 78: 1356-65.
  7. Thickett, D.R. et al. (2002) A role for vascular endothelial growth factor in acute and resolving lung injury.
    Am J Respir Crit Care Med. 166: 1332-7.
  8. Sekine, K. et al. (2006) Panning of multiple subsets of leukocytes on antibody-decorated poly(ethylene) glycol-coated glass slides.
    J Immunol Methods. 313: 96-109.
    9. View The Latest Product References
  9. Ottonello, L. et al. (1999) Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins.
    Blood. 93: 3505-11.
  10. Ottonello, L. et al. (2000) Monoclonal Lym-1 antibody-targeted lysis of B lymphoma cells by neutrophils. Evidence for two mechanisms of FcgammaRII-dependent cytolysis.
    J Leukoc Biol. 68: 662-8.
  11. Moraes, T.J. et al. (2006) Abnormalities in the pulmonary innate immune system in cystic fibrosis.
    Am J Respir Cell Mol Biol. 34: 364-74.
  12. Nijhuis, J. et al. (2009) Neutrophil Activation in Morbid Obesity, Chronic Activation of Acute Inflammation
    Obesity (Silver Spring). 17: 2014-8.
  13. Pliyev, B.K. and Menshikov, M.Y. (2010) Release of the soluble urokinase-type plasminogen activator receptor (suPAR) by activated neutrophils in rheumatoid arthritis.
    Inflammation. 33: 1-9.
  14. Binet, F. and Girard, D. (2008) Novel human neutrophil agonistic properties of arsenic trioxide: involvement of p38 mitogen-activated protein kinase and/or c-jun NH2-terminal MAPK but not extracellular signal-regulated kinases-1/2.
    J Leukoc Biol. 84: 1613-22.
  15. Rosenkranz, A.R. et al. (1999) Novel C5-dependent mechanism of neutrophil stimulation by bioincompatible dialyzer membranes.
    J Am Soc Nephrol. 10: 128-35.
  16. Simard, J.C. et al. (2010) Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism.
    J Leukoc Biol. 87: 905-14.
  17. Sekine, K. et al. (2006) Panning of multiple subsets of leukocytes on antibody-decorated poly(ethylene) glycol-coated glass slides
    J Immunol Methods. 313: 96-109.
  18. Orozco-Uribe, M. et al. (2015) Early Exposure of Human Neutrophils to Mycobacteria Triggers Cell Damage and Pro-Inhibitory Molecules, but not Activation
    Tuberculosis Ch4
  19. Whitmore, L.C. et al. (2016) A Common Genetic Variant in TLR1 Enhances Human Neutrophil Priming and Impacts Length of Intensive Care Stay in Pediatric Sepsis.
    J Immunol. 196 (3): 1376-86.
  20. Bastian, O.W. et al. (2016) Neutrophils contribute to fracture healing by synthesizing fibronectin+ extracellular matrix rapidly after injury.
    Clin Immunol. 164: 78-84.
  21. Dyugovskaya, L. et al. (2016) Intermittent Hypoxia Affects the Spontaneous Differentiation In Vitro of Human Neutrophils into Long-Lived Giant Phagocytes.
    Oxid Med Cell Longev. 2016: 9636937.
  22. Medford, A.J. et al. (2019) Blood-based monitoring identifies acquired and targetable driver HER2 mutations in endocrine-resistant metastatic breast cancer.
    NPJ Precis Oncol. 3: 18.
  23. Ebright, R.Y. et al. (2020) HIF1A signaling selectively supports proliferation of breast cancer in the brain.
    Nat Commun. 11 (1): 6311.
  24. Franses, J.W. et al. (2020) Pancreatic circulating tumor cell profiling identifies LIN28B as a metastasis driver and drug target.
    Nat Commun. 11 (1): 3303.
  25. Bhan, I. et al. (2018) Detection and Analysis of Circulating Epithelial Cells in Liquid Biopsies From Patients With Liver Disease.
    Gastroenterology. 155 (6): 2016-2018.e11.
  26. Fox, D.B. et al. (2023) Downregulation of KEAP1 in melanoma promotes resistance to immune checkpoint blockade.
    NPJ Precis Oncol. 7 (1): 25.

Flow Cytometry

Western Blotting

Synonyms
Biliary Glycoprotein
Carcinoembryonic Antigen Related Cell Adhesion Molecule 8
RRID
AB_2077860
UniProt
P31997
Entrez Gene
CEACAM8
GO Terms
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0006955 immune response
GO:0031225 anchored to membrane

MCA216F

150948 157694 158489 159504 160690 164001 164058

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