CD66b antibody | 80H3
Mouse anti Human CD66b antibody, clone 80H3 (MCA216) used to label neutrophils in human carotid plaques by immunohistochemistry on cryosections.
Image caption:
Immunohistochemical staining of macrophages (CD163+), neutrophils (CD66b+), in human carotid plaques. (A) Distribution of CD163-positive macrophages within area rich in foam cells, (A’) insertion of (A). (B) Distribution of CD66b-positive neutrophils within area rich in foam cells, (B’) insertion of (B).
From: Lavie L, Si-On E, Hoffman A.
Giant phagocytes (Gφ) and neutrophil-macrophage hybrids in human carotid atherosclerotic plaques - An activated phenotype.
Front Immunol. 2023 Feb 24;14:1101569.
doi: 10.3389/fimmu.2023.1101569.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD66b antibody, clone 80H3 (MCA216) used to label neutrophils in human carotid plaques by immunohistochemistry on cryosections.
Image caption:
Immunohistochemical staining for CD66b neutrophils, CD163 macrophages and CD68 foam cells, each stained in a consecutive section of the carotid plaques. (A) a CD66b-positive cell and (B) a CD66b-negative cell are both from the same section stained with anti-CD66b antibody. (C) a CD163-negative and (D) CD163-positive cell, both are from the same section stained with anti-CD163 antibody. In (E, F) CD68-positive cells were stained with anti-CD68 antibody. (G) Isotype control IgG1 for CD66b and CD163. In (H) Isotype control IgG (rabbit) for CD68. (A’–H’) cell magnifications corresponding to (A–H). These sections were taken from a plaque negative for CD66b/CD163 co-localization, as determined by immunofluorescence with confocal microscope. Original magnification: A- H x100, scale bar: 50.2 μm, insert: 2x100, scale bar: 50.2 μm.
From: Lavie L, Si-On E, Hoffman A.
Giant phagocytes (Gφ) and neutrophil-macrophage hybrids in human carotid atherosclerotic plaques - An activated phenotype.
Front Immunol. 2023 Feb 24;14:1101569.
doi: 10.3389/fimmu.2023.1101569.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD66b antibody, clone 80H3 (MCA216) used to label neutrophils in human carotid plaques by immunofluorescence on cryosections.
Image caption:
Representative double immunofluorescence staining for CD66b, CD163, CD68, and MPO in human carotid plaque sections. (A) co-expression of CD66b (red) in neutrophils co-expressing CD68 (green) scavenger receptors, (B) co-expression of CD163 in macrophages (red) co-expressing CD68 (green) scavenger receptors, (C) co-expression of CD66b (red) in neutrophils co-expressing MPO (green), (D) isotype control staining for mouse IgG1 and rabbit IgG followed by CF647 goat anti-mouse IgG (red) and CF488 goat anti-rabbit IgG (green).Top photomicrographs (A–D) represent merged channels of co-expression and enlarged inserts. Below enlarged inserts represent each channel (red or green) separately. Scale bar: 20 μm.
From: Lavie L, Si-On E, Hoffman A.
Giant phagocytes (Gφ) and neutrophil-macrophage hybrids in human carotid atherosclerotic plaques - An activated phenotype.
Front Immunol. 2023 Feb 24;14:1101569.
doi: 10.3389/fimmu.2023.1101569.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD66b antibody, clone 80H3 (MCA216) used to label neutrophils in human carotid plaques by immunofluorescence on cryosections.
Image caption:
Representative double immunofluorescence staining for 3-NT, CD68, CD66b, NE and CD36 in human carotid plaque sections. (A) co-expression of 3-NT (red) and CD68 (green), (B) co-expression of CD66b neutrophils and NE (green), (C) co-expression of 3-NT (red) and NE (green), (D) co-expression of CD66b neutrophil (red) and CD36 scavenger receptor (green). Isotype control staining for mouse IgG and rabbit IgG followed by CF647 goat anti-mouse IgG (red) and CF488 goat anti-rabbit IgG (green), as shown in Figure 4D. Top photomicrographs (A–D) represent merged channels of co-expression and enlarged inserts. Below enlarged inserts represent each channel (red or green) separately. Scale bar: 20 μm.
From: Lavie L, Si-On E, Hoffman A.
Giant phagocytes (Gφ) and neutrophil-macrophage hybrids in human carotid atherosclerotic plaques - An activated phenotype.
Front Immunol. 2023 Feb 24;14:1101569.
doi: 10.3389/fimmu.2023.1101569.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD66b antibody, clone 80H3 (MCA216) used to label neutrophils in human carotid plaques by immunofluorescence on cryosections.
Image caption:
Representative immunofluorescence by double staining of CD66b/CD163, depicting typical co-localizations of various carotid plaques. In (A) merged co-expression of CD66b+/CD163+, in (A’) green channel, higher expression of CD163+ as compared to (A’’) red channel for CD66b. In (B) merged CD66b+/CD163+ co-expression with similar intensities of expression for CD163+ (B’) and CD66b+ (B’’). In (C) merged CD66b+/CD163+co-expression, in (C’) low CD163+ expression, in (C’’) higher expression of CD66b+ as compared to CD163. In (D) isotype controls for rabbit polyclonal antibody (D’) and for mouse monoclonal antibody (D’’).
From: Lavie L, Si-On E, Hoffman A.
Giant phagocytes (Gφ) and neutrophil-macrophage hybrids in human carotid atherosclerotic plaques - An activated phenotype.
Front Immunol. 2023 Feb 24;14:1101569.
doi: 10.3389/fimmu.2023.1101569.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD66b
- Product Type
- Monoclonal Antibody
- Clone
- 80H3
- Isotype
- IgG1
- Specificity
- CD66b
Antibody Quality and Performance Guarantee
| Mouse anti Human CD66b antibody, clone 80H3 reacts with the CEACAM8 or CGM6 gene product, from the carcinoembryonic gene family. The CEACAM8 gene product is called CD66b, Non-specific cross-reacting antigen NCA-95 or Carcinoembryonic antigen CGM6. CD66b is a 286 amino acid mature ~95-100 kDa GPI anchored molecule with an N-terminal 34 amino acid signal peptide and a 29 amino acid C-terminal propeptide, shed in the mature form. CD66b is expressed strongly by mature granulocytes and metamyelocytes, and weakly by bone marrow myelocytes. CD66b is not expressed by peripheral blood monocytes or lymphocytes. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Human leucocytes from individuals with CML.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse MOPC 315-43 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/20 Pack Size: 0.2 mg 1/50 Pack Size: 20 µg |
1/100 |
| Functional Assays 1 | |
||
| Immunohistology - Frozen 2 | |
1/25 | 1/100 |
| Immunohistology - Paraffin | |||
| Western Blotting 3 | |
- 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2 The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 3 See Feuk-Lagerstedt et al.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 5 x 105 cells or 100ul whole blood.
- Histology Positive Control Tissue
- Bone Marrow
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Mannoni, P. et al. (1982) Monoclonal antibodies against human granulocytes and myeloid differentiation antigens.
Hum Immunol. 5 (4): 309-23.
References for CD66b antibody
-
Rosenkranz, A.R. et al. (1999) Novel C5-dependent mechanism of neutrophil stimulation by bioincompatible dialyzer membranes.
J Am Soc Nephrol. 10: 128-35. -
Feuk-Lagerstedt, E. et al. (1999) Identification of CD66a and CD66b as the major galectin-3 receptor candidates in human neutrophils.
J Immunol. 163: 5592-8. -
Ottonello, L. et al. (1999) Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins.
Blood. 93: 3505-11. -
Ottonello, L. et al. (2000) Monoclonal Lym-1 antibody-targeted lysis of B lymphoma cells by neutrophils. Evidence for two mechanisms of FcgammaRII-dependent cytolysis.
J Leukoc Biol. 68: 662-8. -
Jankowski, A. et al. (2002) Determinants of the phagosomal pH in neutrophils.
J Biol Chem. 277: 6059-66. -
Thickett, D.R. et al. (2002) A role for vascular endothelial growth factor in acute and resolving lung injury.
Am J Respir Crit Care Med. 166: 1332-7. -
Jinnouchi, A. et al. (2005) Local anesthetics inhibit priming of neutrophils by lipopolysaccharide for enhanced release of superoxide: suppression of cytochrome b558 expression by disparate mechanisms.
J Leukoc Biol. 78: 1356-65. -
Sekine, K. et al. (2006) Panning of multiple subsets of leukocytes on antibody-decorated poly(ethylene) glycol-coated glass slides.
J Immunol Methods. 313: 96-109.
View The Latest Product References
-
Moraes, T.J. et al. (2006) Abnormalities in the pulmonary innate immune system in cystic fibrosis.
Am J Respir Cell Mol Biol. 34: 364-74. -
Binet, F. and Girard, D. (2008) Novel human neutrophil agonistic properties of arsenic trioxide: involvement of p38 mitogen-activated protein kinase and/or c-jun NH2-terminal MAPK but not extracellular signal-regulated kinases-1/2.
J Leukoc Biol. 84: 1613-22. -
Nijhuis, J. et al. (2009) Neutrophil Activation in Morbid Obesity, Chronic Activation of Acute Inflammation
Obesity (Silver Spring). 17: 2014-8. -
Pliyev, B.K. and Menshikov, M.Y. (2010) Release of the soluble urokinase-type plasminogen activator receptor (suPAR) by activated neutrophils in rheumatoid arthritis.
Inflammation. 33: 1-9. -
Simard, J.C. et al. (2010) Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism.
J Leukoc Biol. 87 (5): 905-14. -
Ionita, M.G. et al. (2010) High neutrophil numbers in human carotid atherosclerotic plaques are associated with characteristics of rupture-prone lesions.
Arterioscler Thromb Vasc Biol. 30 (9): 1842-8. -
Orozco-Uribe, M. et al. (2015) Early Exposure of Human Neutrophils to Mycobacteria Triggers Cell Damage and Pro-Inhibitory Molecules, but not Activation
Tuberculosis Ch4 -
Whitmore, L.C. et al. (2016) A Common Genetic Variant in TLR1 Enhances Human Neutrophil Priming and Impacts Length of Intensive Care Stay in Pediatric Sepsis.
J Immunol. 196 (3): 1376-86. -
Bastian, O.W. et al. (2016) Neutrophils contribute to fracture healing by synthesizing fibronectin+ extracellular matrix rapidly after injury.
Clin Immunol. 164: 78-84. -
Dyugovskaya, L. et al. (2016) Intermittent Hypoxia Affects the Spontaneous Differentiation In Vitro of Human Neutrophils into Long-Lived Giant Phagocytes.
Oxid Med Cell Longev. 2016: 9636937. -
Bhan, I. et al. (2018) Detection and Analysis of Circulating Epithelial Cells in Liquid Biopsies From Patients With Liver Disease.
Gastroenterology. 155 (6): 2016-2018.e11. -
Medford, A.J. et al. (2019) Blood-based monitoring identifies acquired and targetable driver HER2 mutations in endocrine-resistant metastatic breast cancer.
NPJ Precis Oncol. 3: 18. -
Ebright, R.Y. et al. (2020) HIF1A signaling selectively supports proliferation of breast cancer in the brain.
Nat Commun. 11 (1): 6311. -
Franses, J.W. et al. (2020) Pancreatic circulating tumor cell profiling identifies LIN28B as a metastasis driver and drug target.
Nat Commun. 11 (1): 3303. -
Fox, D.B. et al. (2023) Downregulation of KEAP1 in melanoma promotes resistance to immune checkpoint blockade.
NPJ Precis Oncol. 7 (1): 25. -
Dubash, T.D. et al. (2023) Modeling the novel SERD elacestrant in cultured fulvestrant-refractory HR-positive breast circulating tumor cells.
Breast Cancer Res Treat. 201 (1): 43-56. -
Guo, H. et al. (2023) DNA hypomethylation silences anti-tumor immune genes in early prostate cancer and CTCs.
Cell. 186 (13): 2765-2782.e28. -
Sundqvist, M. et al. (2018) Similarities and differences between the responses induced in human phagocytes through activation of the medium chain fatty acid receptor GPR84 and the short chain fatty acid receptor FFA2R.
Biochim Biophys Acta Mol Cell Res. 1865 (5): 695-708. -
Horwitz, E. et al. (2024) CDKN1B (p27kip1) enhances drug tolerant persister CTCs by restricting polyploidy following mitotic inhibitors
bioRχiv. 22 Feb [Epub ahead of print]. -
Saylor, P.J. et al. (2024) Circulating and Imaging Biomarkers of Radium-223 Response in Metastatic Castration-Resistant Prostate Cancer.
JCO Precis Oncol. 8: e2300230. -
Lavie, L. et al. (2023) Giant phagocytes (Gφ) and neutrophil-macrophage hybrids in human carotid atherosclerotic plaques - An activated phenotype.
Front Immunol. 14: 1101569.
- Synonyms
- Biliary Glycoprotein
- Carcinoembryonic Antigen Related Cell Adhesion Molecule 8
- RRID
- AB_2229261
- UniProt
- P31997
- Entrez Gene
- CEACAM8
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0031225 anchored to membrane
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