CD66b antibody | 80H3

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Mouse anti Human CD66b:FITC

Mouse anti Human CD66b

Product Type
Monoclonal Antibody
Clone
80H3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA216F F 100 Tests/2ml
MCA216T C* F 20 µg
MCA216 C* F FN* WB* 0.2 mg
Mouse anti Human CD66b antibody, clone 80H3 reacts with the CEACAM8 or CGM6 gene product, from the carcinoembryonic gene family. The CEACAM8 gene product is called CD66b, Non-specific cross-reacting antigen NCA-95 or Carcinoembryonic antigen CGM6. CD66b is a 286 amino acid mature ~95-100 kDa GPI anchored molecule with an N-terminal 34 amino acid signal peptide and a 29 amino acid C-terminal propeptide, shed in the mature form. CD66b is expressed strongly by mature granulocytes and metamyelocytes, and weakly by bone marrow myelocytes. CD66b is not expressed by peripheral blood monocytes or lymphocytes.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared from ascites or tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Human leucocytes from individuals with CML.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse MOPC 315-43 myeloma cell line.

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P31997 Related reagents
Entrez Gene
CEACAM8 Related reagents
GO Terms
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0006955 immune response
GO:0031225 anchored to membrane
Regulatory
For research purposes only

Applications of CD66b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/50 1/100
Functional Assays 1
Immunohistology - Frozen 2 1/25 1/100
Immunohistology - Paraffin
Western Blotting 3
  1. 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.
  2. 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  3. 3 See Feuk-Lagerstedt et al.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 20ul of the suggested working dilution to label 5 x 105 cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 5 x 105 cells or 100ul whole blood.
Histology Positive Control Tissue
Bone Marrow

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Western Blotting

Product Specific References

References for CD66b antibody

  1. Mannoni, P. et al. (1982) Monoclonal antibodies against human granulocytes and myeloid differentiation antigens.
    Hum Immunol. 5 (4): 309-23.
  2. Ionita, M.G. et al. (2010) High neutrophil numbers in human carotid atherosclerotic plaques are associated with characteristics of rupture-prone lesions.
    Arterioscler Thromb Vasc Biol. 30 (9): 1842-8.
  3. Simard, J.C. et al. (2010) Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism.
    J Leukoc Biol. 87 (5): 905-14.
  4. Feuk-Lagerstedt, E. et al. (1999) Identification of CD66a and CD66b as the major galectin-3 receptor candidates in human neutrophils.
    J Immunol. 163: 5592-8.
  5. Jankowski, A. et al. (2002) Determinants of the phagosomal pH in neutrophils.
    J Biol Chem. 277: 6059-66.
  6. Jinnouchi, A. et al. (2005) Local anesthetics inhibit priming of neutrophils by lipopolysaccharide for enhanced release of superoxide: suppression of cytochrome b558 expression by disparate mechanisms.
    J Leukoc Biol. 78: 1356-65.
  7. Thickett, D.R. et al. (2002) A role for vascular endothelial growth factor in acute and resolving lung injury.
    Am J Respir Crit Care Med. 166: 1332-7.
  8. Sekine, K. et al. (2006) Panning of multiple subsets of leukocytes on antibody-decorated poly(ethylene) glycol-coated glass slides.
    J Immunol Methods. 313: 96-109.
  9. Ottonello, L. et al. (1999) Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins.
    Blood. 93: 3505-11.
  10. Ottonello, L. et al. (2000) Monoclonal Lym-1 antibody-targeted lysis of B lymphoma cells by neutrophils. Evidence for two mechanisms of FcgammaRII-dependent cytolysis.
    J Leukoc Biol. 68: 662-8.
  11. Moraes, T.J. et al. (2006) Abnormalities in the pulmonary innate immune system in cystic fibrosis.
    Am J Respir Cell Mol Biol. 34: 364-74.
  12. Nijhuis, J. et al. (2009) Neutrophil Activation in Morbid Obesity, Chronic Activation of Acute Inflammation
    Obesity (Silver Spring). 17: 2014-8.
  13. Pliyev, B.K. and Menshikov, M.Y. (2010) Release of the soluble urokinase-type plasminogen activator receptor (suPAR) by activated neutrophils in rheumatoid arthritis.
    Inflammation. 33: 1-9.
  14. Binet, F. and Girard, D. (2008) Novel human neutrophil agonistic properties of arsenic trioxide: involvement of p38 mitogen-activated protein kinase and/or c-jun NH2-terminal MAPK but not extracellular signal-regulated kinases-1/2.
    J Leukoc Biol. 84: 1613-22.
  15. Rosenkranz, A.R. et al. (1999) Novel C5-dependent mechanism of neutrophil stimulation by bioincompatible dialyzer membranes.
    J Am Soc Nephrol. 10: 128-35.
  16. Simard, J.C. et al. (2010) Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism.
    J Leukoc Biol. 87: 905-14.
  17. Sekine, K. et al. (2006) Panning of multiple subsets of leukocytes on antibody-decorated poly(ethylene) glycol-coated glass slides
    J Immunol Methods. 313: 96-109.
  18. Orozco-Uribe, M. et al. (2015) Early Exposure of Human Neutrophils to Mycobacteria Triggers Cell Damage and Pro-Inhibitory Molecules, but not Activation
    In: Tuberculosis - Expanding Knowledge. Book edited by Wellman Ribon ISBN 978-953-51-2139-8
  19. Whitmore, L.C. et al. (2016) A Common Genetic Variant in TLR1 Enhances Human Neutrophil Priming and Impacts Length of Intensive Care Stay in Pediatric Sepsis.
    J Immunol. 196 (3): 1376-86.
  20. Bastian, O.W. et al. (2016) Neutrophils contribute to fracture healing by synthesizing fibronectin+ extracellular matrix rapidly after injury.
    Clin Immunol. 164: 78-84.
  21. Dyugovskaya, L. et al. (2016) Intermittent Hypoxia Affects the Spontaneous Differentiation In Vitro of Human Neutrophils into Long-Lived Giant Phagocytes.
    Oxid Med Cell Longev. 2016: 9636937.