CD63 antibody | TEA3/18
|Mouse anti Human CD63 antibody, clone TEA3/18 recognizes the CD63 cell surface antigen, a glycoprotein of ~40-60 kDa.
CD63 is expressed by monocytes and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation.
- Target Species
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- TNF activated HUVEC cells
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood.
de Hass, M. & von dem borne, A.E.G.K. (1997) Platelet antigens. Section Report.
in Kishimoto, T. et al. eds. Leukocyte Typing VI White Cell Differentiation Antigens. Garland Publishing New York pp 625-8.
References for CD63 antibody
Peñas, P.F. et al. (2000) Tetraspanins are localized at motility-related structures and involved in normal human keratinocyte wound healing migration.
J Invest Dermatol. 114 (6): 1126-35.
Casey, T.M. et al. (2007) Organelle proteomics: identification of the exocytic machinery associated with the natural killer cell secretory lysosome.
Mol Cell Proteomics. 6 (5): 767-80.
Cepeda, V. & Fraile-Ramos, A. (2011) A role for the SNARE protein syntaxin 3 in human cytomegalovirus morphogenesis.
Cell Microbiol. 13 (6): 846-58.
Suárez, H. et al. (2017) A bead-assisted flow cytometry method for the semi-quantitative analysis of Extracellular Vesicles.
Sci Rep. 7 (1): 11271.
Oliveira-rodríguez, M. et al. (2016) Development of a rapid lateral flow immunoassay test for detection of exosomes previously enriched from cell culture medium and body fluids.
J Extracell Vesicles. 5: 31803.
Andreu, Z. et al. (2016) Comparative analysis of EV isolation procedures for miRNAs detection in serum samples.
J Extracell Vesicles. 5: 31655.
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