Mouse anti Human CD63 antibody, clone TEA3/18 recognizes the CD63 cell surface antigen, a glycoprotein of ~40-60 kDa.
CD63 is expressed by monocytes and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation.
- Target Species
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- TNF activated HUVEC cells
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to plasma membrane
platelet dense granule membrane
late endosome membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD63 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood.
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
de Hass, M. & von dem borne, A.E.G.K. (1997) Platelet antigens. Section Report.
in Kishimoto, T. et al. eds. Leukocyte Typing VI White Cell Differentiation Antigens. Garland Publishing New York pp 625-8.
References for CD63 antibody
Peñas, P.F. et al. (2000) Tetraspanins are localized at motility-related structures and involved in normal human keratinocyte wound healing migration.
J Invest Dermatol. 114 (6): 1126-35.
Casey, T.M. et al. (2007) Organelle proteomics: identification of the exocytic machinery associated with the natural killer cell secretory lysosome.
Mol Cell Proteomics. 6 (5): 767-80.
Cepeda, V. & Fraile-Ramos, A. (2011) A role for the SNARE protein syntaxin 3 in human cytomegalovirus morphogenesis.
Cell Microbiol. 13 (6): 846-58.
Suárez, H. et al. (2017) A bead-assisted flow cytometry method for the semi-quantitative analysis of Extracellular Vesicles.
Sci Rep. 7 (1): 11271.
Oliveira-rodríguez, M. et al. (2016) Development of a rapid lateral flow immunoassay test for detection of exosomes previously enriched from cell culture medium and body fluids.
J Extracell Vesicles. 5: 31803.
Andreu, Z. et al. (2016) Comparative analysis of EV isolation procedures for miRNAs detection in serum samples.
J Extracell Vesicles. 5: 31655.
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