CD63 antibody | TEA3/18

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Mouse anti Human CD63

Product Type
Monoclonal Antibody
Clone
TEA3/18
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA5672
Datasheet
F
MSDS
0.2 mg loader

Mouse anti Human CD63 antibody, clone TEA3/18 recognizes the CD63 cell surface antigen, a glycoprotein of ~40-60 kDa.

CD63 is expressed by monocytes and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
TNF activated HUVEC cells
Approx. Protein Concentrations
IgG concentration 0.1mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P08962 Related reagents
Entrez Gene
CD63 Related reagents
GO Terms
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005765 lysosomal membrane
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031902 late endosome membrane
Regulatory
For research purposes only

Applications of CD63 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Flow Cytometry

Immuno-electron Microscopy

Product Specific References

Source Reference

  1. de Hass, M. & von dem borne, A.E.G.K. (1997) Platelet antigens. Section Report.
    in Kishimoto, T. et al. eds. Leukocyte Typing VI White Cell Differentiation Antigens. Garland Publishing New York pp 625-8.

References for CD63 antibody

  1. Peñas, P.F. et al. (2000) Tetraspanins are localized at motility-related structures and involved in normal human keratinocyte wound healing migration.
    J Invest Dermatol. 114 (6): 1126-35.
  2. Casey, T.M. et al. (2007) Organelle proteomics: identification of the exocytic machinery associated with the natural killer cell secretory lysosome.
    Mol Cell Proteomics. 6 (5): 767-80.
  3. Cepeda, V. & Fraile-Ramos, A. (2011) A role for the SNARE protein syntaxin 3 in human cytomegalovirus morphogenesis.
    Cell Microbiol. 13 (6): 846-58.
  4. Suárez, H. et al. (2017) A bead-assisted flow cytometry method for the semi-quantitative analysis of Extracellular Vesicles.
    Sci Rep. 7 (1): 11271.
  5. Oliveira-rodríguez, M. et al. (2016) Development of a rapid lateral flow immunoassay test for detection of exosomes previously enriched from cell culture medium and body fluids.
    J Extracell Vesicles. 5: 31803.
  6. Andreu, Z. et al. (2016) Comparative analysis of EV isolation procedures for miRNAs detection in serum samples.
    J Extracell Vesicles. 5: 31655.