CD63 antibody | TEA3/18
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Mouse anti Human CD63
- Product Type
- Monoclonal Antibody
- Clone
- TEA3/18
- Isotype
- IgG1
- Specificity
- CD63
Antibody Quality and Performance Guarantee
| Mouse anti Human CD63 antibody, clone TEA3/18 recognizes the CD63 cell surface antigen, a glycoprotein of ~40-60 kDa. CD63 is expressed by monocytes and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- TNF activated HUVEC cells
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
de Hass, M. & von dem borne, A.E.G.K. (1997) Platelet antigens. Section Report.
in Kishimoto, T. et al. eds. Leukocyte Typing VI White Cell Differentiation Antigens. Garland Publishing New York pp 625-8.
References for CD63 antibody
-
Peñas, P.F. et al. (2000) Tetraspanins are localized at motility-related structures and involved in normal human keratinocyte wound healing migration.
J Invest Dermatol. 114 (6): 1126-35. -
Casey, T.M. et al. (2007) Organelle proteomics: identification of the exocytic machinery associated with the natural killer cell secretory lysosome.
Mol Cell Proteomics. 6 (5): 767-80. -
Cepeda, V. & Fraile-Ramos, A. (2011) A role for the SNARE protein syntaxin 3 in human cytomegalovirus morphogenesis.
Cell Microbiol. 13 (6): 846-58. -
Suárez, H. et al. (2017) A bead-assisted flow cytometry method for the semi-quantitative analysis of Extracellular Vesicles.
Sci Rep. 7 (1): 11271. -
Oliveira-rodríguez, M. et al. (2016) Development of a rapid lateral flow immunoassay test for detection of exosomes previously enriched from cell culture medium and body fluids.
J Extracell Vesicles. 5: 31803. -
Andreu, Z. et al. (2016) Comparative analysis of EV isolation procedures for miRNAs detection in serum samples.
J Extracell Vesicles. 5: 31655.
- RRID
- AB_10709460
- UniProt
- P08962
- Entrez Gene
- CD63
- GO Terms
- GO:0002576 platelet degranulation
- GO:0005887 integral to plasma membrane
- GO:0005765 lysosomal membrane
- GO:0030168 platelet activation
- GO:0031088 platelet dense granule membrane
- GO:0031902 late endosome membrane
MCA5672
152352
159057
162717
180222
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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