CD62P antibody | Psel.KO.2.7

Mouse anti Human CD62P

Product Type
Monoclonal Antibody
Clone
Psel.KO.2.7
Isotype
IgG1
Specificity
CD62P

Product Code Applications Pack Size List Price Your Price Qty
MCA2419
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.2 mg loader
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loader
MCA2419T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti human CD62P, clone Psel.KO.2.7, recognizes human P-Selectin. CD62P is a ~140 kDa surface antigen expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Horse
Bovine
Rat
Goat
Cat
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
P-selectin transfected 300.19 cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised CD62P knock-out mice (strain C57/B6) were fused with cells of the NS-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Immunohistology - Frozen (See ref/ 1)
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD62P antibody

  1. Massaguer, A. et al. (2000) Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
    Tissue Antigens. 56 (2): 117-28.
  2. Massaguer, A. et al. (2003) Characterization of platelet and soluble-porcine P-selectin (CD62P).
    Vet Immunol Immunopathol. 96 (3-4): 169-81.
  3. Massaguer, A. et al. (2002) Reactivity of CD62P workshop mAbs with resting and activated platelets from different animal species.
    In: Leucocyte Typing VII. Edited by Mason, D. et al. Oxford University Press, pp 342-3.
  4. Shirasuna, K. et al. (2012) Rapid accumulation of polymorphonuclear neutrophils in the Corpus luteum during prostaglandin F(2α)-induced luteolysis in the cow.
    PLoS One. 7: e29054.
  5. Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35: 602-13.
  6. Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).
    Vet Immunol Immunopathol. 164 (1-2): 87-92.
  7. Johnson, C.A. Jr et al. (2008) Flow cytometric assays for quantifying activated ovine platelets.
    Artif Organs. 32 (2): 136-45.
  8. Johnson, C.A. Jr et al. (2011) Biocompatibility assessment of the first generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35 (1): 9-21.
  9. View The Latest Product References
  10. Johnson, C.A. Jr et al. (2011) Platelet activation after implantation of the Levitronix PediVAS in the ovine model.
    ASAIO J. 57 (6): 516-21.
  11. Shankarraman, V. et al. (2014) Biocompatibility Assessment of the CentriMag-Novalung Adult ECMO Circuit in a Model of Acute Pulmonary Hypertension.
    ASAIO J. 60 (4): 429-35.
  12. Trichler, S.A. et al. (2013) Ultra-pure platelet isolation from canine whole blood.
    BMC Vet Res. 9: 144.
  13. Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).
    Vet Immunol Immunopathol. 164 (1-2): 87-92.
  14. Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35 (6): 602-13.
  15. ChanC, H.H. et al. (2017) Shear Stress-Induced Total Blood Trauma in Multiple Species.
    Artif Organs. 41 (10): 934-947.
  16. Theuerkauf, K. et al. (2022) Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome.
    J Vet Diagn Invest. 10406387221077969.
  17. Acar, D.D. et al. (2016) Upregulation of endothelial cell adhesion molecules characterizes veins close to granulomatous infiltrates in the renal cortex of cats with feline infectious peritonitis and is indirectly triggered by feline infectious peritonitis virus-infected monocytes in vitro.
    J Gen Virol. 97 (10): 2633-2642.
  18. Prat, N.J. et al. (2015) Low-Dose Heparin Anticoagulation During Extracorporeal Life Support for Acute Respiratory Distress Syndrome in Conscious Sheep.
    Shock. 44 (6): 560-8.

Flow Cytometry

Functional Assays

Synonyms
P-SELECTIN
RRID
AB_566866
UniProt
P16109
P42201
P98106
Entrez Gene
SELP
SELP
Selp
GO Terms
GO:0001530 lipopolysaccharide binding
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0008201 heparin binding
GO:0050829 defense response to Gram-negative bacterium
GO:0009897 external side of plasma membrane
GO:0010572 positive regulation of platelet activation
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031092 platelet alpha granule membrane
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0042806 fucose binding
GO:0043208 glycosphingolipid binding
GO:0070492 oligosaccharide binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005529 sugar binding
GO:0002691 regulation of cellular extravasation
GO:0006954 inflammatory response
GO:0014070 response to organic cyclic compound
GO:0043499 eukaryotic cell surface binding
GO:0045785 positive regulation of cell adhesion
GO:0048514 blood vessel morphogenesis
View more products with CD62 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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