CD62P antibody | Psel.KO.2.12

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Anti Human CD62P Antibody, clone Psel.KO.2.12 gallery image 1 — Figure A. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B . FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 647 conjugated Mouse anti Human CD62P (MCA2420A647). All experiments performed on human blood gated on single platelets activated with thrombin, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer

Mouse anti Human CD62P:Alexa Fluor® 488

Mouse anti Human CD62P:Alexa Fluor® 647

Mouse anti Human CD62P

Mouse anti Human CD62P:RPE

Product Type: Monoclonal Antibody
Clone: Psel.KO.2.12
Isotype: IgG1

Product Code	Applications	Pack Size
MCA2420A488T	F	25 Tests/0.25ml
MCA2420A488	F	100 Tests/1ml
MCA2420A647	F	100 Tests/1ml
MCA2420T	C F IP	25 µg
MCA2420	C F IP	0.2 mg
MCA2420PET	F	25 Tests
MCA2420PE	F	100 Tests

Mouse anti Human CD62P antibody, clone Psel.KO.2.12 recognizes the CD62P cell surface antigen, a ~140 kDa glycoprotein also known as P-selectin.

CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.

Mouse anti Human CD62P antibody, clone Psel.KO.2.12 inhibits P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells (Massaguer et al. 2000).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Goat
Rat
Sheep

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Alexa Fluor® 488 - liquid

Product Form

Purified IgG conjugated to Alexa Fluor 647 - liquid

Product Form

Purified IgG - liquid

Product Form

Pack Size: 25 Tests

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised

Pack Size: 100 Tests

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Pack Size: 25 Tests

Reconstitute with 0.25 ml distilled water

Pack Size: 100 Tests

Reconstitute with 1.0 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Carrier Free

Yes

Immunogen

CD62P transfected 300.19 cells.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised CD62P knock-out mice (Strain C57/B6) were fused with cells of the NS-1 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Pack Size: 25 Tests
Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Pack Size: 100 Tests
Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution.

More Information

UniProt: P16109 Related reagents
Entrez Gene: SELP Related reagents
GO Terms: GO:0001530 lipopolysaccharide binding; GO:0002576 platelet degranulation; GO:0005887 integral to plasma membrane; GO:0005615 extracellular space; GO:0005624 membrane fraction; GO:0005625 soluble fraction; GO:0008201 heparin binding; GO:0050829 defense response to Gram-negative bacterium; GO:0009897 external side of plasma membrane; GO:0010572 positive regulation of platelet activation; GO:0030168 platelet activation; GO:0031088 platelet dense granule membrane; GO:0031092 platelet alpha granule membrane; GO:0032496 response to lipopolysaccharide; GO:0033691 sialic acid binding; GO:0042806 fucose binding; GO:0043208 glycosphingolipid binding; GO:0070492 oligosaccharide binding
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD62P antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/5
Flow Cytometry	Neat	1/2
Flow Cytometry	1/50	1/100
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry		Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:Alexa Fluor® 488	MCA928A488	100 Tests/1ml	F
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	100 Tests/1ml	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD62P antibody

Massaguer, A. et al. (2000) Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
Tissue Antigens. 56 (2): 117-28.
Massaguer, A. et al. (2003) Characterization of platelet and soluble-porcine P-selectin (CD62P).
Vet Immunol Immunopathol. 96 (3-4): 169-81.
Massaguer, A. et al. (2002) Reactivity of CD62P workshop mAbs with resting and activated platelets from different animal species.
In: Leucocyte Typing VII. Edited by Mason, D. et al. Oxford University Press, pp 342-3.
Major, T.C. et al. (2010) The attenuation of platelet and monocyte activation in a rabbit model of extracorporeal circulation by a nitric oxide releasing polymer.
Biomaterials. 31: 2736-45.
Johnson, C.A. Jr. et al. (2008) Flow cytometric assays for quantifying activated ovine platelets.
Artif Organs. 32: 136-45.
Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
Artif Organs. 35 (6): 602-13.
Johnson, C.A. Jr. et al. (2011) Biocompatibility assessment of the first generation PediaFlow pediatric ventricular assist device.
Artif Organs. 35 (1): 9-21.
Dasse, K.A. et al. (2007) Assessment of hydraulic performance and biocompatibility of a MagLev centrifugal pump system designed for pediatric cardiac or cardiopulmonary support.
ASAIO J. 53 (6): 771-7.
Ding, J. et al. (2015) Quantification of Shear-Induced Platelet Activation: High Shear Stresses for Short Exposure Time.
Artif Organs. 39 (7): 576-83.
Tran GT et al. (2010) Membrane attack complex of complement is not essential for immune mediated demyelination in experimental autoimmune neuritis.
J Neuroimmunol. 229 (1-2): 98-106.
Foruzanmehr, M. et al. (2014) Nano-structure TiO₂ film coating on 316L stainless steel via sol-gel technique for blood compatibility improvement.
Nanomedicine Journal 1 (3): 128-36.

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