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CD62P antibody | Psel.KO.2.12

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Anti Human CD62P Antibody, clone Psel.KO.2.12 gallery image 1
Staining of thrombin activated platelets with Mouse anti Human CD62P:RPE (MCA2420PET)

Mouse anti Human CD62P:Alexa Fluor® 488

Mouse anti Human CD62P:Alexa Fluor® 647

Mouse anti Human CD62P

Mouse anti Human CD62P:RPE

Product Type
Monoclonal Antibody
Clone
Psel.KO.2.12
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2420A488T F 25 Tests/0.25ml
MCA2420A488 F 100 Tests/1ml
MCA2420A647 F 100 Tests/1ml
MCA2420T C F IP 25 µg
MCA2420 C F IP 0.2 mg
MCA2420PET F 25 Tests
MCA2420PE F 100 Tests
Mouse anti Human CD62P antibody, clone Psel.KO.2.12 recognizes the CD62P cell surface antigen, a ~140 kDa glycoprotein also known as P-selectin.

CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.

Mouse anti Human CD62P antibody, clone Psel.KO.2.12 inhibits P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells (Massaguer et al. 2000).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Rat
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG - liquid
Product Form
Pack Size: 25 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Pack Size: 100 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 25 Tests
Reconstitute with 0.25 ml distilled water
Pack Size: 100 Tests
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
CD62P transfected 300.19 cells.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised CD62P knock-out mice (Strain C57/B6) were fused with cells of the NS-1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 25 Tests
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P16109 Related reagents
Entrez Gene
SELP Related reagents
GO Terms
GO:0001530 lipopolysaccharide binding
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0008201 heparin binding
GO:0050829 defense response to Gram-negative bacterium
GO:0009897 external side of plasma membrane
GO:0010572 positive regulation of platelet activation
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031092 platelet alpha granule membrane
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0042806 fucose binding
GO:0043208 glycosphingolipid binding
GO:0070492 oligosaccharide binding
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD62P antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Flow Cytometry Neat
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 100 Tests/1ml F
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 100 Tests/1ml F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD62P antibody

  1. Massaguer, A. et al. (2000) Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
    Tissue Antigens. 56 (2): 117-28.
  2. Massaguer, A. et al. (2003) Characterization of platelet and soluble-porcine P-selectin (CD62P).
    Vet Immunol Immunopathol. 96 (3-4): 169-81.
  3. Massaguer, A. et al. (2002) Reactivity of CD62P workshop mAbs with resting and activated platelets from different animal species.
    In: Leucocyte Typing VII. Edited by Mason, D. et al. Oxford University Press, pp 342-3.
  4. Major, T.C. et al. (2010) The attenuation of platelet and monocyte activation in a rabbit model of extracorporeal circulation by a nitric oxide releasing polymer.
    Biomaterials. 31: 2736-45.
  5. Johnson, C.A. Jr. et al. (2008) Flow cytometric assays for quantifying activated ovine platelets.
    Artif Organs. 32: 136-45.
  6. Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35 (6): 602-13.
  7. Johnson, C.A. Jr. et al. (2011) Biocompatibility assessment of the first generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35 (1): 9-21.
  8. Dasse, K.A. et al. (2007) Assessment of hydraulic performance and biocompatibility of a MagLev centrifugal pump system designed for pediatric cardiac or cardiopulmonary support.
    ASAIO J. 53 (6): 771-7.
  9. Ding, J. et al. (2015) Quantification of Shear-Induced Platelet Activation: High Shear Stresses for Short Exposure Time.
    Artif Organs. 39 (7): 576-83.
  10. Tran GT et al. (2010) Membrane attack complex of complement is not essential for immune mediated demyelination in experimental autoimmune neuritis.
    J Neuroimmunol. 229 (1-2): 98-106.
  11. Foruzanmehr, M. et al. (2014) Nano-structure TiO2 film coating on 316L stainless steel via sol-gel technique for blood compatibility improvement.
    Nanomedicine Journal 1 (3): 128-36.
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