CD62P antibody | Psel.KO.2.12
Mouse anti Human CD62P
- Product Type
- Monoclonal Antibody
- Clone
- Psel.KO.2.12
- Isotype
- IgG1
- Specificity
- CD62P
Antibody Quality and Performance Guarantee
| Mouse anti Human CD62P antibody, clone Psel.KO.2.12 recognizes the CD62P cell surface antigen, a ~140 kDa glycoprotein also known as P-selectin. CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. Mouse anti Human CD62P antibody, clone Psel.KO.2.12 inhibits P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells (Massaguer et al. 2000). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Rat Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- CD62P transfected 300.19 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized CD62P knock-out mice (Strain C57/B6) were fused with cells of the NS-1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Massaguer, A. et al. (2000) Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
Tissue Antigens. 56 (2): 117-28.
References for CD62P antibody
-
Massaguer, A. et al. (2002) Reactivity of CD62P workshop mAbs with resting and activated platelets from different animal species.
In: Leucocyte Typing VII. Edited by Mason, D. et al. Oxford University Press, pp 342-3. -
Dasse, K.A. et al. (2007) Assessment of hydraulic performance and biocompatibility of a MagLev centrifugal pump system designed for pediatric cardiac or cardiopulmonary support.
ASAIO J. 53 (6): 771-7. -
Johnson, C.A. Jr. et al. (2008) Flow cytometric assays for quantifying activated ovine platelets.
Artif Organs. 32: 136-45. -
Tran GT et al. (2010) Membrane attack complex of complement is not essential for immune mediated demyelination in experimental autoimmune neuritis.
J Neuroimmunol. 229 (1-2): 98-106. -
Major, T.C. et al. (2010) The attenuation of platelet and monocyte activation in a rabbit model of extracorporeal circulation by a nitric oxide releasing polymer.
Biomaterials. 31: 2736-45. -
Johnson, C.A. Jr. et al. (2011) Biocompatibility assessment of the first generation PediaFlow pediatric ventricular assist device.
Artif Organs. 35 (1): 9-21. -
Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
Artif Organs. 35 (6): 602-13. -
Ding, J. et al. (2015) Quantification of Shear-Induced Platelet Activation: High Shear Stresses for Short Exposure Time.
Artif Organs. 39 (7): 576-83.
View The Latest Product References
-
Foruzanmehr, M. et al. (2014) Nano-structure TiO2 film coating on 316L stainless steel via sol-gel technique for blood compatibility improvement.
Nanomedicine Journal 1 (3): 128-36.
- Synonyms
- P-SELECTIN
- RRID
- AB_566867
- UniProt
- P16109
- Entrez Gene
- SELP
- GO Terms
- GO:0001530 lipopolysaccharide binding
- GO:0002576 platelet degranulation
- GO:0005887 integral to plasma membrane
- GO:0005615 extracellular space
- GO:0005624 membrane fraction
- GO:0005625 soluble fraction
- GO:0008201 heparin binding
- GO:0050829 defense response to Gram-negative bacterium
- GO:0009897 external side of plasma membrane
- View More GO Terms
- GO:0010572 positive regulation of platelet activation
- GO:0030168 platelet activation
- GO:0031088 platelet dense granule membrane
- GO:0031092 platelet alpha granule membrane
- GO:0032496 response to lipopolysaccharide
- GO:0033691 sialic acid binding
- GO:0042806 fucose binding
- GO:0043208 glycosphingolipid binding
- GO:0070492 oligosaccharide binding
MCA2420
1214
166485
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