CD62E antibody | CL2/6

Mouse anti Human CD62E

Product Type
Monoclonal Antibody
Clone
CL2/6
Isotype
IgG2a
Specificity
CD62E

Product Code Applications Pack Size List Price Your Price Qty
MCA1969
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD62E antibody, clone CL2/6 recognizes human CD62E, a member of the selectin family which is expressed on endothelial cells at sites of inflammation.

Mouse anti Human CD62E antibody, clone CL2/6 has been shown to inhibit the binding of human neutrophils to stimulated HUVECS.

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20
Immunofluorescence
Immunohistology - Frozen 0.5ug/ml 2.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD62E antibody

  1. Abbassi, O. et al. (1993) E-selectin supports neutrophil rolling in vitro under conditions of flow.
    J Clin Invest. 92 (6): 2719-30.
  2. Kishimoto, T.K. et al. (1991) Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro.
    Blood. 78: 805-11.
  3. Marin, C. et al. (2011) Mediterranean diet reduces endothelial damage and improves the regenerative capacity of endothelium.
    Am J Clin Nutr. 93: 267-74.
  4. Rius, C. et al. (2010) Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.
    J Immunol. 185: 3718-27.
  5. Wassmer, S.C. et al. (2011) Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF.
    Cell Microbiol. 13: 198-209.
  6. Nieuwland, R. et al. (2000) Cellular origin and procoagulant properties of microparticles in meningococcal sepsis.
    Blood. 95: 930-5.
  7. Lo. C. Y. et al. (2013) The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling
    Biomaterials. 34 (33): 8213-22.

Flow Cytometry

Synonyms
E-SELECTIN
RRID
AB_2186827
UniProt
P16581
Entrez Gene
SELE
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0002092 positive regulation of receptor internalization
GO:0002523 leukocyte migration involved in inflammatory response
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005615 extracellular space
GO:0005901 caveola
GO:0005905 coated pit
GO:0007202 activation of phospholipase C activity
GO:0019722 calcium-mediated signaling
GO:0030029 actin filament-based process
GO:0030863 cortical cytoskeleton
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0034612 response to tumor necrosis factor
GO:0043274 phospholipase binding
GO:0048471 perinuclear region of cytoplasm
GO:0050727 regulation of inflammatory response
GO:0050901 leukocyte tethering or rolling
GO:0070492 oligosaccharide binding
GO:0070555 response to interleukin-1
View more products with CD62 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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