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CD62E antibody | CL2/6

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Anti Human CD62E Antibody, clone CL2/6 gallery image 1
Published customer image:
Mouse anti Human CD62e antibody, clone CL2/6 (MCA1969) used to affect CHO-P cell adhesion to selectin bearing substrate under shear stress conditions.
Image caption:
MSC-PPG-19Fc[FUT7+] binding to P- and E-selectin in microfluidic flow chamber A-B. 2-5×106 MSCs/mL immobilized with either 100 μg/mL 19Fc[FUT7+], 100-200 μg/mL anti-P-selectin mAb G1 or 100-200 μg/mL anti-E-selectin mAb CL2/6 were perfused over either P-selectin bearing CHO-P cells at a wall shear stress of 1 dyn/cm2 (panel A) or E-selectin bearing stimulated HUVECs at 2 dyn/cm2 (panel B). Only the MSC-PPG-19Fc[FUT7+] cells bound the selectin bearing substrate. Cell interaction was specifically blocked using anti-PSGL-1 mAb KPL1, anti-P-selectin mAb G1 or anti-E-selectin mAb CL2/6 as indicated. Data are mean ± SD for 3-4 independent experiments. * indicates P <0.001 with respect to all other conditions. C. Cumulative rolling velocity plot showing median rolling velocity of 3 μm/s when MSC-PPG-19Fc[FUT7+] roll on P-selectin/CHO-P cells at 1 dyn/cm2 and 11.5 μm/s for rolling on E-selectin/ HUVECs at 2 dyn/cm2. The rolling characteristics of 190 cells were analyzed and binned from three independent runs.

From: Lo CY, Antonopoulos A, Dell A, Haslam SM, Lee T, Neelamegham S.
The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling.
Biomaterials. 2013 Nov;34(33):8213-22.

Mouse anti Human CD62E:Biotin

Mouse anti Human CD62E:FITC

Mouse anti Human CD62E

Product Type
Monoclonal Antibody
Clone
CL2/6
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1969B
Datasheet
F
SDS
100 Tests loader

MCA1969F
Datasheet
F
SDS
100 Tests loader

MCA1969
Datasheet
C F IF
SDS
0.1 mg loader

Mouse anti Human CD62E antibody, clone CL2/6 recognizes human CD62E, a member of the selectin family which is expressed on endothelial cells at sites of inflammation.

Mouse anti Human CD62E antibody, clone CL2/6 has been shown to inhibit the binding of human neutrophils to stimulated HUVECS.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.02%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P16581
Entrez Gene
SELE
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0002092 positive regulation of receptor internalization
GO:0002523 leukocyte migration involved in inflammatory response
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005615 extracellular space
GO:0005901 caveola
GO:0005905 coated pit
GO:0007202 activation of phospholipase C activity
GO:0019722 calcium-mediated signaling
GO:0030029 actin filament-based process
GO:0030863 cortical cytoskeleton
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0034612 response to tumor necrosis factor
GO:0043274 phospholipase binding
GO:0048471 perinuclear region of cytoplasm
GO:0050727 regulation of inflammatory response
GO:0050901 leukocyte tethering or rolling
GO:0070492 oligosaccharide binding
GO:0070555 response to interleukin-1
Regulatory
For research purposes only

Applications of CD62E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Flow Cytometry
Flow Cytometry 1/20
Immunofluorescence
Immunohistology - Frozen 0.5ug/ml 2.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Product Specific References

References for CD62E antibody

  1. Abbassi, O. et al. (1993) E-selectin supports neutrophil rolling in vitro under conditions of flow.
    J Clin Invest. 92 (6): 2719-30.
  2. Kishimoto, T.K. et al. (1991) Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro.
    Blood. 78: 805-11.
  3. Marin, C. et al. (2011) Mediterranean diet reduces endothelial damage and improves the regenerative capacity of endothelium.
    Am J Clin Nutr. 93: 267-74.
  4. Rius, C. et al. (2010) Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.
    J Immunol. 185: 3718-27.
  5. Wassmer, S.C. et al. (2011) Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF.
    Cell Microbiol. 13: 198-209.
  6. Nieuwland, R. et al. (2000) Cellular origin and procoagulant properties of microparticles in meningococcal sepsis.
    Blood. 95: 930-5.
  7. Lo. C. Y. et al. (2013) The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling
    Biomaterials. 34 (33): 8213-22.

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