CD62E antibody | CL2/6

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Anti Human CD62E Antibody, clone CL2/6 gallery image 1 — **Published customer image:**
Mouse anti Human CD62e antibody, clone CL2/6 (**MCA1969**) used to affect CHO-P cell adhesion to selectin bearing substrate under shear stress conditions.
**Image caption:**
MSC-PPG-19Fc[FUT7⁺] binding to P- and E-selectin in microfluidic flow chamber A-B. 2-5×10⁶ MSCs/mL immobilized with either 100 μg/mL 19Fc[FUT7⁺], 100-200 μg/mL anti-P-selectin mAb G1 or 100-200 μg/mL anti-E-selectin mAb CL2/6 were perfused over either P-selectin bearing CHO-P cells at a wall shear stress of 1 dyn/cm² (panel A) or E-selectin bearing stimulated HUVECs at 2 dyn/cm² (panel B). Only the MSC-PPG-19Fc[FUT7⁺] cells bound the selectin bearing substrate. Cell interaction was specifically blocked using anti-PSGL-1 mAb KPL1, anti-P-selectin mAb G1 or anti-E-selectin mAb CL2/6 as indicated. Data are mean ± SD for 3-4 independent experiments. * indicates P <0.001 with respect to all other conditions. C. Cumulative rolling velocity plot showing median rolling velocity of 3 μm/s when MSC-PPG-19Fc[FUT7⁺] roll on P-selectin/CHO-P cells at 1 dyn/cm² and 11.5 μm/s for rolling on E-selectin/ HUVECs at 2 dyn/cm². The rolling characteristics of 190 cells were analyzed and binned from three independent runs.

From: Lo CY, Antonopoulos A, Dell A, Haslam SM, Lee T, Neelamegham S.
The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling.
Biomaterials. 2013 Nov;34(33):8213-22.

Mouse anti Human CD62E:Biotin

Mouse anti Human CD62E:FITC

Mouse anti Human CD62E

Product Type: Monoclonal Antibody
Clone: CL2/6
Isotype: IgG2a

Product Code	Applications	Pack Size
MCA1969B	F	100 Tests
MCA1969F	F	100 Tests
MCA1969	C F IF	0.1 mg

Mouse anti Human CD62E antibody, clone CL2/6 recognizes human CD62E, a member of the selectin family which is expressed on endothelial cells at sites of inflammation.

Mouse anti Human CD62E antibody, clone CL2/6 has been shown to inhibit the binding of human neutrophils to stimulated HUVECS.

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Biotin - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Buffer Solution

TRIS buffered saline

Buffer Solution

TRIS buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.02%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P16581 Related reagents
Entrez Gene: SELE Related reagents
GO Terms: GO:0007157 heterophilic cell-cell adhesion; GO:0002092 positive regulation of receptor internalization; GO:0002523 leukocyte migration involved in inflammatory response; GO:0016021 integral to membrane; GO:0004888 transmembrane receptor activity; GO:0005615 extracellular space; GO:0005901 caveola; GO:0005905 coated pit; GO:0007202 activation of phospholipase C activity; GO:0019722 calcium-mediated signaling; GO:0030029 actin filament-based process; GO:0030863 cortical cytoskeleton; GO:0032496 response to lipopolysaccharide; GO:0033691 sialic acid binding; GO:0034612 response to tumor necrosis factor; GO:0043274 phospholipase binding; GO:0048471 perinuclear region of cytoplasm; GO:0050727 regulation of inflammatory response; GO:0050901 leukocyte tethering or rolling; GO:0070492 oligosaccharide binding; GO:0070555 response to interleukin-1
Regulatory: For research purposes only

Applications of CD62E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry
Flow Cytometry
Flow Cytometry		1/20
Immunofluorescence
Immunohistology - Frozen	0.5ug/ml	2.0ug/ml

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG2a:FITC	HCA037F	0.1 mg	F
Human anti Mouse IgG2a:HRP	HCA037P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG2a Negative Control:FITC	MCA929F	100 Tests	F
Mouse IgG2a Negative Control	MCA929	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD62E antibody

Abbassi, O. et al. (1993) E-selectin supports neutrophil rolling in vitro under conditions of flow.
J Clin Invest. 92 (6): 2719-30.
Kishimoto, T.K. et al. (1991) Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro.
Blood. 78: 805-11.
Marin, C. et al. (2011) Mediterranean diet reduces endothelial damage and improves the regenerative capacity of endothelium.
Am J Clin Nutr. 93: 267-74.
Rius, C. et al. (2010) Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.
J Immunol. 185: 3718-27.
Wassmer, S.C. et al. (2011) Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF.
Cell Microbiol. 13: 198-209.
Nieuwland, R. et al. (2000) Cellular origin and procoagulant properties of microparticles in meningococcal sepsis.
Blood. 95: 930-5.
Lo. C. Y. et al. (2013) The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling
Biomaterials. 34 (33): 8213-22.

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