CD62E antibody | CL2/6
Mouse anti Human CD62e antibody, clone CL2/6 (MCA1969) used to affect CHO-P cell adhesion to selectin bearing substrate under shear stress conditions.
Image caption:
MSC-PPG-19Fc[FUT7+] binding to P- and E-selectin in microfluidic flow chamber A-B. 2-5×106 MSCs/mL immobilized with either 100 μg/mL 19Fc[FUT7+], 100-200 μg/mL anti-P-selectin mAb G1 or 100-200 μg/mL anti-E-selectin mAb CL2/6 were perfused over either P-selectin bearing CHO-P cells at a wall shear stress of 1 dyn/cm2 (panel A) or E-selectin bearing stimulated HUVECs at 2 dyn/cm2 (panel B). Only the MSC-PPG-19Fc[FUT7+] cells bound the selectin bearing substrate. Cell interaction was specifically blocked using anti-PSGL-1 mAb KPL1, anti-P-selectin mAb G1 or anti-E-selectin mAb CL2/6 as indicated. Data are mean ± SD for 3-4 independent experiments. * indicates P <0.001 with respect to all other conditions. C. Cumulative rolling velocity plot showing median rolling velocity of 3 μm/s when MSC-PPG-19Fc[FUT7+] roll on P-selectin/CHO-P cells at 1 dyn/cm2 and 11.5 μm/s for rolling on E-selectin/ HUVECs at 2 dyn/cm2. The rolling characteristics of 190 cells were analyzed and binned from three independent runs.
From: Lo CY, Antonopoulos A, Dell A, Haslam SM, Lee T, Neelamegham S.
The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling.
Biomaterials. 2013 Nov;34(33):8213-22.
Mouse anti Human CD62E
- Product Type
- Monoclonal Antibody
- Clone
- CL2/6
- Isotype
- IgG2a
- Specificity
- CD62E
Antibody Quality and Performance Guarantee
| Mouse anti Human CD62E antibody, clone CL2/6 recognizes human CD62E, a member of the selectin family which is expressed on endothelial cells at sites of inflammation. Mouse anti Human CD62E antibody, clone CL2/6 has been shown to inhibit the binding of human neutrophils to stimulated HUVECS. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/20 | |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
0.5ug/ml | 2.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for CD62E antibody
-
Kishimoto, T.K. et al. (1991) Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro.
Blood. 78: 805-11. -
Abbassi, O. et al. (1993) E-selectin supports neutrophil rolling in vitro under conditions of flow.
J Clin Invest. 92 (6): 2719-30. -
Nieuwland, R. et al. (2000) Cellular origin and procoagulant properties of microparticles in meningococcal sepsis.
Blood. 95: 930-5. -
Rius, C. et al. (2010) Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.
J Immunol. 185: 3718-27. -
Marin, C. et al. (2011) Mediterranean diet reduces endothelial damage and improves the regenerative capacity of endothelium.
Am J Clin Nutr. 93: 267-74. -
Wassmer, S.C. et al. (2011) Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF.
Cell Microbiol. 13: 198-209. -
Lo. C. Y. et al. (2013) The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling
Biomaterials. 34 (33): 8213-22.
- Synonyms
- E-SELECTIN
- RRID
- AB_2186827
- UniProt
- P16581
- Entrez Gene
- SELE
- GO Terms
- GO:0007157 heterophilic cell-cell adhesion
- GO:0002092 positive regulation of receptor internalization
- GO:0002523 leukocyte migration involved in inflammatory response
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0005615 extracellular space
- GO:0005901 caveola
- GO:0005905 coated pit
- GO:0007202 activation of phospholipase C activity
- View More GO Terms
- GO:0019722 calcium-mediated signaling
- GO:0030029 actin filament-based process
- GO:0030863 cortical cytoskeleton
- GO:0032496 response to lipopolysaccharide
- GO:0033691 sialic acid binding
- GO:0034612 response to tumor necrosis factor
- GO:0043274 phospholipase binding
- GO:0048471 perinuclear region of cytoplasm
- GO:0050727 regulation of inflammatory response
- GO:0050901 leukocyte tethering or rolling
- GO:0070492 oligosaccharide binding
- GO:0070555 response to interleukin-1
MCA1969
150081
155221
156973
161009
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