CD61 antibody | Y2/51
Alexafluor 647 conjugated Mouse anti Human CD61 antibody, clone Y2/51 (MCA2588A647) used for the assessment of CD61 expression by platelets by immunofluorescence.
Image caption:
Minocycline reduces activation of human platelets in vitro.
Platelets from healthy, seronegative donors were isolated and activation was assessed via (A) measurement of sCD40L levels by ELISA after treatment with thrombin alone or together with minocycline, or (B) measurement of CD62P (P selectin; a platelet activation marker) median fluorescent intensity (MFI) by flow cytometry. Data from (B) is presented as fold change of CD62P MFI normalized to non-treated (NT) samples (visualized by horizontal black line set at 1). Data sets for (A) were compared using a one way ANOVA statistical test and data sets for (B) were compared using a two way ANOVA statistical test followed by Bonferroni's test for multiple comparisons, where * denotes p<0.05. Data shown is representative of three separate experiments. (C) Platelet degranulation was assessed via measurement of mepacrine retention by flow cytometry. The data is representative of three separate experiments and was compared using a one way ANOVA statistical test followed by Bonferroni's test for multiple comparisons, where * denotes p<0.05. (D) Representative images of mepacrine retention in platelet granules as analyzed by Amnis Image Stream following the indicated treatments. CD61 marks the platelet surface (in red), and mepacrine is stored in platelet dense granules (in green).
From: Jackson JW, Singh MV, Singh VB, Jones LD, Davidson GA, Ture S, et al. (2016)
Novel Antiplatelet Activity of Minocycline Involves Inhibition of MLK3-p38 Mitogen Activated Protein Kinase Axis.
PLoS ONE 11(6): e0157115.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD61:Alexa Fluor® 488
Mouse anti Human CD61:Alexa Fluor® 647
Mouse anti Human CD61:FITC
Mouse anti Human CD61
Mouse anti Human CD61:RPE
- Product Type
- Monoclonal Antibody
- Clone
- Y2/51
- Isotype
- IgG1
Mouse anti Human CD61 antibody, clone Y2/51 recognizes CD61, a ~105 kDa glycoprotein, also known as integrin beta 3 chain. This molecule associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51.
CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin.
CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor®488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- Pack Size: 25 µg, 0.2 mg
0.09% Sodium Azide (NaN3) Pack Size: 0.1 mg0.09% Sodium Azide (NaN3) - Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- PHA stimulated peripheral blood cells.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P05106 Related reagents
- Entrez Gene
- ITGB3 Related reagents
- GO Terms
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0002576 platelet degranulation
- GO:0050839 cell adhesion molecule binding
- GO:0004872 receptor activity
- GO:0005161 platelet-derived growth factor receptor binding
- GO:0008305 integrin complex
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007411 axon guidance
- GO:0042802 identical protein binding
- GO:0010595 positive regulation of endothelial cell migration
- GO:0010745 negative regulation of macrophage derived foam cell differentiation
- GO:0010888 negative regulation of lipid storage
- GO:0014909 smooth muscle cell migration
- GO:0030168 platelet activation
- GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
- GO:0031092 platelet alpha granule membrane
- GO:0032147 activation of protein kinase activity
- GO:0032369 negative regulation of lipid transport
- GO:0035295 tube development
- GO:0043184 vascular endothelial growth factor receptor 2 binding
- GO:0044419 interspecies interaction between organisms
- GO:0045124 regulation of bone resorption
- GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050748 negative regulation of lipoprotein metabolic process
- GO:0050900 leukocyte migration
- GO:0060055 angiogenesis involved in wound healing
- GO:0071062 alphav-beta3 integrin-vitronectin complex
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD61 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 Pack Size: 25 Tests/0.25ml 1:2 Pack Size: 100 Tests/1ml |
|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/100 | 1/200 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Western Blotting | |||
| Flow Cytometry | Neat |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin prior to staining.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | 100 Tests/1ml | F | ||
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | 100 Tests/1ml | F | ||
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F | ||
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F | ||
| Mouse IgG1 Negative Control:RPE | MCA928PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for CD61 antibody
-
Phillips, D.R. et al. (1991) GPIIb-IIIa: the responsive integrin.
Cell. 65 (3): 359-62. -
Hynes, R.O. (1992) Integrins: versatility, modulation, and signaling in cell adhesion.
Cell. 69 (1): 11-25. -
Michelson, A. D. et al. (1995) A panel of platelet mAb for the study of haemostasis and thrombosis in baboons.
Leucocyte Typing V. Oxford University Press p 1230-1. -
Gatter, K.C. et al. (1988) The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens.
Histopathology. 13 (3): 257-67. -
Kamat, V. et al. (2015) Microfluidic assessment of functional culture-derived platelets in human thrombi under flow.
Exp Hematol. 43 (10): 891-900.e4. -
Ogino, T. et al. (2014) Erythroid and megakaryocytic differentiation of K562 erythroleukemic cells by monochloramine.
Free Radic Res. 48 (3): 292-302. -
Tehrani, S. et al. (2010) Atorvastatin has antithrombotic effects in patients with type 1 diabetes and dyslipidemia.
Thromb Res. 126 (3): e225-31. -
Dircks, B.H. et al. (2012) Platelet-neutrophil aggregate formation in blood samples from dogs with systemic inflammatory disorders.
Am J Vet Res. 73 (7): 939-45. -
Comazzi, S. et al. (2010) Acute megakaryoblastic leukemia in dogs: a report of three cases and review of the literature.
J Am Anim Hosp Assoc. 46 (5): 327-35. -
Jackson, J.W. et al. (2016) Novel Antiplatelet Activity of Minocycline Involves Inhibition of MLK3-p38 Mitogen Activated Protein Kinase Axis.
PLoS One. 11 (6): e0157115. -
Chen, C.P. et al. (2018) Oxidative stress reduces trophoblast FOXO1 and integrin β3 expression that inhibits cell motility.
Free Radic Biol Med. Jun 07 [Epub ahead of print].
