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CD61 antibody | Y2/51

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Anti Human CD61 Antibody, clone Y2/51 thumbnail image 1 Anti Human CD61 Antibody, clone Y2/51 thumbnail image 2 Anti Human CD61 Antibody, clone Y2/51 thumbnail image 3
Anti Human CD61 Antibody, clone Y2/51 gallery image 1
Figure A. RPE conjugated Mouse anti Human CD49b (MCA2025PE) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. RPE conjugated Mouse anti Human CD49b (MCA2025PE) and Alexa Fluor 647 conjugated Mouse anti Human CD61 (MCA2588A647). All experiments performed on red cell lysed human blood gated on platelets in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Human CD61 Antibody, clone Y2/51 gallery image 2
Published customer image:
Alexafluor 647 conjugated Mouse anti Human CD61 antibody, clone Y2/51 (MCA2588A647) used for the assessment of CD61 expression by platelets by immunofluorescence.
Image caption:
Minocycline reduces activation of human platelets in vitro.
Platelets from healthy, seronegative donors were isolated and activation was assessed via (A) measurement of sCD40L levels by ELISA after treatment with thrombin alone or together with minocycline, or (B) measurement of CD62P (P selectin; a platelet activation marker) median fluorescent intensity (MFI) by flow cytometry. Data from (B) is presented as fold change of CD62P MFI normalized to non-treated (NT) samples (visualized by horizontal black line set at 1). Data sets for (A) were compared using a one way ANOVA statistical test and data sets for (B) were compared using a two way ANOVA statistical test followed by Bonferroni's test for multiple comparisons, where * denotes p<0.05. Data shown is representative of three separate experiments. (C) Platelet degranulation was assessed via measurement of mepacrine retention by flow cytometry. The data is representative of three separate experiments and was compared using a one way ANOVA statistical test followed by Bonferroni's test for multiple comparisons, where * denotes p<0.05. (D) Representative images of mepacrine retention in platelet granules as analyzed by Amnis Image Stream following the indicated treatments. CD61 marks the platelet surface (in red), and mepacrine is stored in platelet dense granules (in green).

From: Jackson JW, Singh MV, Singh VB, Jones LD, Davidson GA, Ture S, et al. (2016)
Novel Antiplatelet Activity of Minocycline Involves Inhibition of MLK3-p38 Mitogen Activated Protein Kinase Axis.
PLoS ONE 11(6): e0157115.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD61 Antibody, clone Y2/51 gallery image 3
FFPE human spleen stained with Mouse Anti-Human CD61 (MCA2588), biotinylated Anti-Mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.

Mouse anti Human CD61:Alexa Fluor® 488

Mouse anti Human CD61:Alexa Fluor® 647

Mouse anti Human CD61:FITC

Mouse anti Human CD61

Mouse anti Human CD61:RPE

Product Type
Monoclonal Antibody
Clone
Y2/51
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2588A488T F 25 Tests/0.25ml
MCA2588A488 F 100 Tests/1ml
MCA2588A647T F 25 Tests/0.25ml
MCA2588A647 F 100 Tests/1ml
MCA2588F F 0.1 mg
MCA2588T C F P* WB 25 µg
MCA2588GA C F P* 0.1 mg
MCA2588 C F IF P* WB 0.2 mg
MCA2588PE F 100 Tests/1ml
Mouse anti Human CD61 antibody, clone Y2/51 recognizes CD61, a ~105 kDa glycoprotein, also known as integrin beta 3 chain. This molecule associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51.

CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor®488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
Pack Size: 25 µg, 0.2 mg
0.09%Sodium Azide (NaN3)
Pack Size: 0.1 mg
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
PHA stimulated peripheral blood cells.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P05106 Related reagents
Entrez Gene
ITGB3 Related reagents
GO Terms
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002576 platelet degranulation
GO:0050839 cell adhesion molecule binding
GO:0004872 receptor activity
GO:0005161 platelet-derived growth factor receptor binding
GO:0008305 integrin complex
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0010595 positive regulation of endothelial cell migration
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010888 negative regulation of lipid storage
GO:0014909 smooth muscle cell migration
GO:0030168 platelet activation
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0031092 platelet alpha granule membrane
GO:0032147 activation of protein kinase activity
GO:0032369 negative regulation of lipid transport
GO:0035295 tube development
GO:0043184 vascular endothelial growth factor receptor 2 binding
GO:0044419 interspecies interaction between organisms
GO:0045124 regulation of bone resorption
GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050748 negative regulation of lipoprotein metabolic process
GO:0050900 leukocyte migration
GO:0060055 angiogenesis involved in wound healing
GO:0071062 alphav-beta3 integrin-vitronectin complex
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD61 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10 Pack Size: 25 Tests/0.25ml
1:2 Pack Size: 100 Tests/1ml
Flow Cytometry Neat 1/10
Flow Cytometry 1/100 1/200
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting
Flow Cytometry Neat
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin prior to staining.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 100 Tests/1ml F
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 100 Tests/1ml F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD61 antibody

  1. Phillips, D.R. et al. (1991) GPIIb-IIIa: the responsive integrin.
    Cell. 65 (3): 359-62.
  2. Hynes, R.O. (1992) Integrins: versatility, modulation, and signaling in cell adhesion.
    Cell. 69 (1): 11-25.
  3. Michelson, A. D. et al. (1995) A panel of platelet mAb for the study of haemostasis and thrombosis in baboons.
    Leucocyte Typing V. Oxford University Press p 1230-1.
  4. Gatter, K.C. et al. (1988) The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens.
    Histopathology. 13 (3): 257-67.
  5. Kamat, V. et al. (2015) Microfluidic assessment of functional culture-derived platelets in human thrombi under flow.
    Exp Hematol. 43 (10): 891-900.e4.
  6. Ogino, T. et al. (2014) Erythroid and megakaryocytic differentiation of K562 erythroleukemic cells by monochloramine.
    Free Radic Res. 48 (3): 292-302.
  7. Tehrani, S. et al. (2010) Atorvastatin has antithrombotic effects in patients with type 1 diabetes and dyslipidemia.
    Thromb Res. 126 (3): e225-31.
  8. Dircks, B.H. et al. (2012) Platelet-neutrophil aggregate formation in blood samples from dogs with systemic inflammatory disorders.
    Am J Vet Res. 73 (7): 939-45.
  9. Comazzi, S. et al. (2010) Acute megakaryoblastic leukemia in dogs: a report of three cases and review of the literature.
    J Am Anim Hosp Assoc. 46 (5): 327-35.
  10. Jackson, J.W. et al. (2016) Novel Antiplatelet Activity of Minocycline Involves Inhibition of MLK3-p38 Mitogen Activated Protein Kinase Axis.
    PLoS One. 11 (6): e0157115.
  11. Chen, C.P. et al. (2018) Oxidative stress reduces trophoblast FOXO1 and integrin β3 expression that inhibits cell motility.
    Free Radic Biol Med. Jun 07 [Epub ahead of print].
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