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CD59 antibody | MEM-43

Product Code Applications Pack Size List Price Your Price Qty
MCA1054GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E EM F IF IP P * WB * 0.1 mg loader
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loader
MCA1054
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E EM F IF IP P * WB * 0.2 mg loader
List Price Your Price
loader

Mouse anti Human CD59 antibody, clone MEM-43 recognizes CD59, a glycosyl-phosphatidylinositol (GPI) anchored membrane protein also known as membrane attack complex inhibition factor. CD59 blocks the formation of the complement membrane attack complex (MAC) by binding of C8a and C9. CD59 is found on all types of leucocytes including platelets and is also expressed on many non-haematopoietic cells.
The epitope recognized by Mouse anti Human CD59 antibody, clone MEM-43 is lost after reduction therefore, non-reducing conditions are required for western blotting techniques.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
Thymocytes and T lymphocytes.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/25 1/100
Immuno-electron Microscopy
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting 2
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
  2. 2This product recognizes CD59 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl
Histology Positive Control Tissue
Tonsil

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
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Description Mouse IgG2a Negative Control

References for CD59 antibody

  1. Horejsí, V. et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
    Folia Biol (Praha). 34 (1): 23-34.
  2. Stefanová, I. et al. (1989) Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol.
    Mol Immunol. 26 (2): 153-61.
  3. Stefanová, I. et al. (1989) in Leucocyte Typing IV: White cell differentiation antigens.
    Ed. Knapp, W. et al. Oxford University Press pp 678-97.
  4. Stefanová, I. & Horejsí, V. (1991) Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules.
    J Immunol. 147 (5): 1587-92.
  5. Tandon, N. et al. (1994) Expression and function of multiple regulators of complement activation in autoimmune thyroid disease.
    Immunology. 81 (4): 643-7.
  6. Vanderplasschen, A. et al. (1997) Extracellular enveloped vaccinia virus is resistant to complement because of incorporation of host complement control proteins into its envelope.
    Proc Natl Acad Sci U S A. 95: 7544-9.
  7. Cowan, P.J. et al. (1998) High-level endothelial expression of human CD59 prolongs heart function in an ex vivo model of xenograft rejection.
    Transplantation. 65: 826-31.
  8. Chong, Y.H. and Lee, M.J. (2000) Expression of complement inhibitor protein CD59 in human neuronal and glial cell lines treated with HIV-1 gp41 peptides.
    J Neurovirol. 6: 51-60.
  9. View The Latest Product References
  10. Shamri, R. et al. (2002) Chemokine stimulation of lymphocyte alpha 4 integrin avidity but not of leukocyte function-associated antigen-1 avidity to endothelial ligands under shear flow requires cholesterol membrane rafts.
    J Biol Chem. 277: 40027-35.
  11. Zhang, J. et al. (2002) Early complement activation and decreased levels of glycosylphosphatidylinositol-anchored complement inhibitors in human and experimental diabetic retinopathy.
    Diabetes. 51: 3499-504.
  12. Donin, N. et al. (2003) Complement resistance of human carcinoma cells depends on membrane regulatory proteins, protein kinases and sialic acid.
    Clin Exp Immunol. 131: 254-63.
  13. Gendek-Kubiak, H. and Gendek, E.G. (2004) Immunolocalization of protectin (CD59) and macrophages in polymyositis and dermatomyositis.
    J Neuroimmunol. 149: 187-94.
  14. Jolly, C, and Sattentau. Q.J. (2005) Human Immunodeficiency Virus Type 1 Virological Synapse Formation in T Cells Requires Lipid Raft Integrity
    J Virol. 79: 12088-94.
  15. Ohyama, M. et al. (2006) Characterization and isolation of stem cell-enriched human hair follicle bulge cells.
    J Clin Invest. 116: 249-60.
  16. Ellison, B.S. et al. (2007) Complement susceptibility in glutamine deprived breast cancer cells.
    Cell Div. 2007 2: 20.
  17. Takemoto, M. et al. (2007) Human herpesvirus 7 infection increases the expression levels of CD46 and CD59 in target cells.
    J Gen Virol. 88: 1415-22.
  18. Shaw, M.L. et al. (2008) Cellular proteins in influenza virus particles.
    PLoS Pathog. 4: e1000085.
  19. Bonnon, C. et al. (2010) Selective export of human GPI-anchored proteins from the endoplasmic reticulum.
    J Cell Sci. 123: 1705-15.
  20. Sadallah, S. et al. (2011) Microparticles (ectosomes) shed by stored human platelets downregulate macrophages and modify the development of dendritic cells.
    J Immunol. 186: 6543-52.
  21. Rondelli, T. et al. (2013) The frequency of granulocytes with spontaneous somatic mutations: a wide distribution in a normal human population.
    PLoS One. 8 (1): e54046.
  22. Abe, Y. et al. (2017) Glycan region of GPI anchored-protein is required for cytocidal oligomerization of an anticancer parasporin-2, Cry46Aa1 protein, from Bacillus thuringiensis strain A1547.
    J Invertebr Pathol. 142: 71-81.
  23. Sica, M. et al. (2017) Eculizumab treatment: stochastic occurrence of C3 binding to individual PNH erythrocytes.
    J Hematol Oncol. 10 (1): 126.
  24. Gullipalli, D. et al. (2018) Antibody Inhibition of Properdin Prevents Complement-Mediated Intravascular and Extravascular Hemolysis.
    J Immunol. 201 (3): 1021-1029.
  25. Ueda, M. et al. (2019) Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity.
    Mol Cell Endocrinol. 490: 57-67.

Synonyms
HRF
Protectin
RRID
AB_321508
UniProt
P13987
Entrez Gene
CD59
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0007166 cell surface receptor linked signaling pathway
GO:0031362 anchored to external side of plasma membrane

MCA1054GA

0515 166484

MCA1054

160915 1804

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