CD59 antibody | MEM-43

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Anti Human CD59 Antibody, clone MEM-43 thumbnail image 1

Anti Human CD59 Antibody, clone MEM-43 thumbnail image 2

Anti Human CD59 Antibody, clone MEM-43 thumbnail image 3

Anti Human CD59 Antibody, clone MEM-43 gallery image 1 — Figure A. FITC conjugated Mouse anti Human CD3 (MCA463F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and RPE conjugated Mouse anti Human CD59 (MCA1054PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Anti Human CD59 Antibody, clone MEM-43 gallery image 2 — Published customer image:
Mouse anti Human CD59 antibody, clone MEM-43 used for the detection of CD59 in virus infected cell lysates by western blotting.
Image caption:
The effect of protease treatment on influenza virion associated host proteins. Purified influenza A/WSN/33 virus was either mock treated or subjected to overnight digestion with subtilisin followed by concentration through a sucrose cushion. 10 ug of mock infected cell lysate (lane 1) or influenza infected cell lysate (lane 2) and 2 ug of untreated influenza virions (lane 3) or protease treated influenza virions (lane 4) were then analyzed by western blot with antibodies against the indicated proteins. Numbers to the right are molecular weight markers.

From: Shaw ML, Stone KL, Colangelo CM, Gulcicek EE, Palese P (2008) Cellular Proteins in Influenza Virus Particles. PLoS Pathog 4(6): e1000085.

Anti Human CD59 Antibody, clone MEM-43 gallery image 3 — Published customer image:
R-Phycoerythrin and Alexa Fluor 647 conjugated Mouse anti Human CD59 antibodies, clone MEM-43 (MCA1054PE and MCA1054A647) used for the evaluation of CD59 expression on human erythrocytes by flow cytometry.
Image caption:
In vivo C3 binding on red cells of PNH patients on eculizumab. a. Kinetics of C3 binding on red cells from a representative PNH patient during eculizumab treatment. Before treatment (0 week), no red cell binds C3, whereas starting from 1 week of treatment three populations of red cells are displayed: one population of normal red cells (CD59 + C3−) and two distinct populations of PNH (CD59-negative) red cells, one with (C3+) and one without (C3−) fragments bound on their surface. b. Characterization of C3 fragment bound to erythrocytes from PNH patient on eculizumab. The left panel shows the normal (CD59-positive) and PNH (CD59-negative) populations (gated by the elliptic mark). The PNH (CD59-negative) population (see gate in the left panel) has been analyzed with anti-C3d and either anti-C3b (middle panel) or anti-iC3b (left panel). SSC: side scatter

From: Sica M, Rondelli T, Ricci P, De Angioletti M, Risitano AM, Notaro R.
Eculizumab treatment: stochastic occurrence of C3 binding to individual PNH erythrocytes.
J Hematol Oncol. 2017 Jun 19;10(1):126.

Mouse anti Human CD59:Alexa Fluor® 488

Mouse anti Human CD59:Alexa Fluor® 647

Mouse anti Human CD59:Preservative Free

Mouse anti Human CD59:FITC

Mouse anti Human CD59

Mouse anti Human CD59:RPE

Product Type: Monoclonal Antibody
Clone: MEM-43
Isotype: IgG2a

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1054A488	F			100 Tests/1ml
MCA1054A647T	F			25 Tests/0.25ml
MCA1054A647	F			100 Tests/1ml
MCA1054XZ	C E F IF IP P* WB*			1 mg
MCA1054FT	F			25 µg
MCA1054F	F			0.1 mg
MCA1054T	C E EM F IF IP P* WB*			25 µg
MCA1054GA	C E EM F IF IP P* WB*			0.1 mg
MCA1054	C E EM F IF IP P* WB*			0.2 mg
MCA1054PET	F			25 Tests/0.25ml
MCA1054PE	F			100 Tests

Mouse anti Human CD59 antibody, clone MEM-43 recognizes CD59, a glycosyl-phosphatidylinositol (GPI) anchored membrane protein also known as membrane attack complex inhibition factor. CD59 blocks the formation of the complement membrane attack complex (MAC) by binding of C8a and C9. CD59 is found on all types of leucocytes including platelets and is also expressed on many non-haematopoietic cells.
The epitope recognized by Mouse anti Human CD59 antibody, clone MEM-43 is lost after reduction therefore, non-reducing conditions are required for western blotting techniques.

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Alexa Fluor® 488 - liquid

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Pack Size: 25 Tests/0.25ml

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised

Pack Size: 100 Tests

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Pack Size: 25 Tests/0.25ml

Reconstitute with 0.25 ml distilled water

Pack Size: 100 Tests

Reconstitute with 1.0 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

None present

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

Pack Size: 25 Tests/0.25ml

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Pack Size: 100 Tests

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Immunogen

Thymocytes and T lymphocytes.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Pack Size: 25 Tests/0.25ml
Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Pack Size: 100 Tests
Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P13987 Related reagents
Entrez Gene: CD59 Related reagents
GO Terms: GO:0005515 protein binding; GO:0007596 blood coagulation; GO:0005576 extracellular region; GO:0005624 membrane fraction; GO:0007166 cell surface receptor linked signaling pathway; GO:0031362 anchored to external side of plasma membrane
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Regulatory: For research purposes only

Applications of CD59 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat
Flow Cytometry			Neat
ELISA
Flow Cytometry		1/5	1/10
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin ¹
Immunoprecipitation
Western Blotting ²
Flow Cytometry			Neat
ELISA
Flow Cytometry		1/5	1/10
Immuno-electron Microscopy
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin ¹
Immunoprecipitation
Western Blotting ²
Flow Cytometry			Neat

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
²This product recognizes CD59 under non-reducing conditions.

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
²This product recognizes CD59 under non-reducing conditions.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Histology Positive Control Tissue: Tonsil
Histology Positive Control Tissue: Tonsil

Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Human anti Mouse IgG2a:FITC	HCA037F	0.1 mg	F
Human anti Mouse IgG2a:HRP	HCA037P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F
Human anti Mouse IgG2a:FITC	HCA037F	0.1 mg	F
Human anti Mouse IgG2a:HRP	HCA037P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 488	MCA929A488	100 Tests/1ml	F
Mouse IgG2a Negative Control:Alexa Fluor® 647	MCA929A647	100 Tests/1ml	F
Mouse IgG2a Negative Control	MCA929	100 Tests	F
Mouse IgG2a Negative Control:FITC	MCA929F	100 Tests	F
Mouse IgG2a Negative Control	MCA929	100 Tests	F
Mouse IgG2a Negative Control:RPE	MCA929PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Immuno-electron Microscopy

Immunofluorescence

Immunohistology - Frozen

Western Blotting

Product Specific References

References for CD59 antibody

Stefanova, I. et al. (1989) in Leucocyte Typing IV: White cell differentiation antigens.
Ed. Knapp, W. et al. Oxford University Press pp 678-97.
Stefanová, I. et al. (1989) Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol.
Mol Immunol. 26 (2): 153-61.
Tandon, N. et al. (1994) Expression and function of multiple regulators of complement activation in autoimmune thyroid disease.
Immunology. 81 (4): 643-7.
Horejsí, V. et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
Folia Biol (Praha). 34 (1): 23-34.
Stefanová, I. & Horejsí, V. (1991) Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules.
J Immunol. 147 (5): 1587-92.
Shaw, M.L. et al. (2008) Cellular proteins in influenza virus particles.
PLoS Pathog. 4: e1000085.
Sadallah, S. et al. (2011) Microparticles (ectosomes) shed by stored human platelets downregulate macrophages and modify the development of dendritic cells.
J Immunol. 186: 6543-52.
Jolly, C, and Sattentau. Q.J. (2005) Human Immunodeficiency Virus Type 1 Virological Synapse Formation in T Cells Requires Lipid Raft Integrity
J Virol. 79: 12088-94.
Shamri, R. et al. (2002) Chemokine stimulation of lymphocyte alpha 4 integrin avidity but not of leukocyte function-associated antigen-1 avidity to endothelial ligands under shear flow requires cholesterol membrane rafts.
J Biol Chem. 277: 40027-35.
Bonnon, C. et al. (2010) Selective export of human GPI-anchored proteins from the endoplasmic reticulum.
J Cell Sci. 123: 1705-15.
Zhang, J. et al. (2002) Early complement activation and decreased levels of glycosylphosphatidylinositol-anchored complement inhibitors in human and experimental diabetic retinopathy.
Diabetes. 51: 3499-504.
Ellison, B.S. et al. (2007) Complement susceptibility in glutamine deprived breast cancer cells.
Cell Div. 2007 2: 20.
Cowan, P.J. et al. (1998) High-level endothelial expression of human CD59 prolongs heart function in an ex vivo model of xenograft rejection.
Transplantation. 65: 826-31.
Vanderplasschen, A. et al. (1997) Extracellular enveloped vaccinia virus is resistant to complement because of incorporation of host complement control proteins into its envelope.
Proc Natl Acad Sci U S A. 95: 7544-9.
Takemoto, M. et al. (2007) Human herpesvirus 7 infection increases the expression levels of CD46 and CD59 in target cells.
J Gen Virol. 88: 1415-22.
Chong, Y.H. and Lee, M.J. (2000) Expression of complement inhibitor protein CD59 in human neuronal and glial cell lines treated with HIV-1 gp41 peptides.
J Neurovirol. 6: 51-60.
Gendek-Kubiak, H. and Gendek, E.G. (2004) Immunolocalization of protectin (CD59) and macrophages in polymyositis and dermatomyositis.
J Neuroimmunol. 149: 187-94.
Abe, Y. et al. (2017) Glycan region of GPI anchored-protein is required for cytocidal oligomerization of an anticancer parasporin-2, Cry46Aa1 protein, from Bacillus thuringiensis strain A1547.
J Invertebr Pathol. 142: 71-81.
Sica, M. et al. (2017) Eculizumab treatment: stochastic occurrence of C3 binding to individual PNH erythrocytes.
J Hematol Oncol. 10 (1): 126.

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