CD58 antibody | BRIC5
Mouse anti Human CD58
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD58 antibody, clone BRIC5 recognizes human Lymphocyte function-associated antigen 3, also known as CD58 or LFA-3. CD58 is a 250 amino acid single pass type I transmembrane glycoprotein, a member of the immunoglobulin superfamily, with a predicted molecular mass of 28.1 kDa and an apparent molecular mass of ~55-70 kDa. CD58 occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA and is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes (Makgoba et al. 1989).CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.
Mouse anti Human CD58 antibody, clone BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108 M-1. It reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is pronase sensitive. Mouse anti Human CD58 antibody, clone BRIC5 inhibits T cell rosetting.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Human erythrocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD58 antibody
Makgoba, M.W. et al. (1989) The CD2-LFA-3 and LFA-1-ICAM pathways: relevance to T-cell recognition.
Immunol Today. 10 (12): 417-22.
Shaw, S. and Johnson, J.P. (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens.
Edited by Knapp, W., Dorken, B., Gilks, W.R., Rieber, E.P., Schmidt, R.E., Stein, H. and von dem Borne, A.E.G.Kr. Oxford University Press. pp 714-716.
Grundy, J.E. et al. (1993) Increased adherence of CD2 peripheral blood lymphocytes to cytomegalovirus-infected fibroblasts is blocked by anti-LFA-3 antibody.
Immunology. 78 (3): 413-20.
Fletcher, J.M. et al. (1998) Natural killer cell lysis of cytomegalovirus (CMV)-infected cells correlates with virally induced changes in cell surface lymphocyte function-associated antigen-3 (LFA-3) expression and not with the CMV-induced down-regulation of cell surface class I HLA.
J Immunol. 161 (5): 2365-74.
Bergmann-leitner, E.S. & Abrams, S.I. (2000) Differential role of Fas/Fas ligand interactions in cytolysis of primary and metastatic colon carcinoma cell lines by human antigen-specific CD8+ CTL.
J Immunol. 164 (9): 4941-54.
Cerboni, C. et al. (2000) Human cytomegalovirus strain-dependent changes in NK cell recognition of infected fibroblasts.
J Immunol. 164 (9): 4775-82.
Abbate, I. et al. (2001) Changes in host cell molecules acquired by circulating HIV-1 in patients treated with highly active antiretroviral therapy and interleukin-2.
AIDS. 15 (1): 11-6.
Kato, T. et al. (2002) Salivary cystatins induce interleukin-6 expression via cell surface molecules in human gingival fibroblasts.
Mol Immunol. 39 (7-8): 423-30.
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Kanuga, N. et al. (2002) Characterization of genetically modified human retinal pigment epithelial cells developed for in vitro and transplantation studies.
Invest Ophthalmol Vis Sci. 43 (2): 546-55.
Bottley, G. et al. (2005) Differential expression of LFA-3, Fas and MHC Class I on Ad5- and Ad12-transformed human cells and their susceptibility to lymphokine-activated killer (LAK) cells.
Virology. 338 (2): 297-308.
Abbate, I. et al. (2005) Cell membrane proteins and quasispecies compartmentalization of CSF and plasma HIV-1 from aids patients with neurological disorders.
Infect Genet Evol. 5 (3): 247-53.
Pandolfino, M.C. et al. (2010) Comparison of three culture media for the establishment of melanoma cell lines.
Cytotechnology. 62 (5): 403-12.
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