CD56 antibody | MEM-188
FITC conjugated Mouse anti Human CD56 antibody, clone MEM-188 (MCA2046F) used for the identificationof CD56 positive cells from human peripheral blood mononuclear cell populations by flow cytometry.
Image caption:
Intracellular GM-CSF staining on CD56+ cells from hPBMC. (A) In the presence of the indicated ODN plus IL2: GM-CSF content in the cell culture without ODN stimulation was used as a control. Data are shown as mean +SD and are representative of three similar experiments. A specific response was defined as a response where the percentage of GM-CSF in CD56+ lymphocytes was at least 2-fold below the background observed in the medium control. The asterisk indicates statistically significant differences (p<0.05). (B) In the presence of IL2 in both CD3+ (NKT) and CD3- (NK) CD56+ dim or bright populations: ODNs and/or IL2 were added to the medium to reach a final concentration of 6 μg/ml and 400 IU/ml respectively. Dot plots are representative of three similar experiments.
From: Rodriguez JM, Marchicio J, López M, Ziblat A, Elias F, et al. (2015) PyNTTTTGT and CpG Immunostimulatory Oligonucleotides: Effect on Granulocyte/Monocyte Colony-Stimulating Factor (GM-CSF) Secretion by Human CD56+ (NK and NKT) Cells. PLoS ONE 10(2): e0117484.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
AlexaFluor-647 conjugated Mouse anti Human CD56 antibody (MCA2046A647) used for detection of CD56 on peripheral blood mononuclear cells by flow cytoimetry.
Image caption:
NK Cells Do Not Respond to iRBCs in the Absence of Accessory Cells PBMCs or MACS-purified NK cells from malaria-naïve donors were cultured alone or with IL-12 and IL-18, uRBCs, or live iRBCs for 24 h. Intracellular IFN-γ and surface expression of CD69 on CD3− CD56+ NK cells were analysed by flow cytometry. (A and B) Representative example of NK responses to uRBCs, IL-12 and IL-18, and iRBCs in PBMCs (A) and purified NK cells (B). FACS plots are gated on CD3− CD56+ lymphocytes. Percentages indicate the proportion of CD3− CD56+ NK cells in each gate; data are based on the collection of 100,000 total events. (C and D) Comparison of NK responses to iRBCs in PBMCs and in purified NK cells. (C) NK cell IFN-γ production for six donors. (D) CD69 surface expression levels for seven donors. **, p <0.01; ***, p <0.001; p-values are for paired t tests comparing stimulated and unstimulated NK cells from the same donor, or PBMCs and pure NK cells from the same donor. (E) MACS-purified, CTG-labelled NK cells were added to autologous PBMCs. IFN-γ production in CTG+ (MACS-purified) and CTG− (“untouched”) NK cells was assessed by flow cytometry after 24 h of stimulation with iRBCs. A representative example of the software gating strategy to analyse IFN-γ responses of CTG-labelled NK cells is shown. FACS plots were gated on CD3− CD56+ NK cells within the lymphocyte population (first panel), and NK cells were analysed for CTG staining (second panel). CTG+ CD3− CD56+ NK cells (third panel) or CTG− CD3− CD56+ NK cells (fourth panel) were selected and analysed for intracellular IFN-γ production. Numbers indicate the proportion of IFN-γ+ NK cells.
From: Newman KC, Korbel DS, Hafalla JC, Riley EM (2006)
Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-γ Responses to Malaria.
PLoS Pathog 2(12): e118.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
AlexaFluor-647 conjugated Mouse anti Human CD56 antibody used for detection of CD56 on peripheral blood mononuclear cells by flow cytoimetry.
Image caption:
HLA-DR+ Accessory Cells Provide Activating Signals to NK Cells PBMCs from malaria-naïve donors were depleted of different populations of accessory cells, and NK cell responses to iRBCs were analysed after 24 h of co-culture. (A) Representative example of NK cell IFN-γ responses in undepleted PBMCs (top left), PBMCs depleted of all HLA-DR+ antigen-presenting cells (APC) (top right), CD14+ monocyte–depleted PBMCs (middle left), CD19+ B cell–depleted PBMCs (middle right), CD1c+ mDC–depleted PBMCs (bottom left), and BDCA-4+ pDC–depleted PBMCs (bottom right). FACS plots are gated on CD3− CD56+ lymphocytes. Percentages indicate the proportion of CD3− CD56+ NK cells that were positive for IFN-γ; data are based on the collection of 100,000 total events. (B) NK cell IFN-γ production after depletion of different antigen-presenting cell populations. Percentage of IFN-γ NK cells relative to the response in undepleted PBMCs is shown; each spot represents data for a different donor. *, p < 0.05; ***, p < 0.0001; p–values are for paired t tests of the difference between depleted and undepleted cells:
From: Newman KC, Korbel DS, Hafalla JC, Riley EM (2006) Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-γ Responses to Malaria. PLoS Pathog 2(12): e118.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD56 antibody, clone MEM-188 recognizes CD56 a 140kDa cell surface glycoprotein expressed primarily by natural killer cells and a subset of T cells in the peripheral blood.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
Applications of CD56 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/25 | 1/50 Pack Size: 0.1 mg 1/100 Pack Size: 0.2 mg |
|
| Immunohistology - Frozen 1 | 1/40 | ||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |||
| Western Blotting 2 | Non-reducing conditions |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2 MEM-188 recognizes CD56 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Product Specific References
References for CD56 antibody
-
Costa, P.et al (1997) CD56 Workshop Panel Report.
In: Leucocyte typing VI. White cell differentiation antigens. Garland Publishing Inc. New York and London. 271-2. -
Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.
J Immunol. 166 (4): 2364-71. -
Staffler G et al. (2003) Selective inhibition of T cell activation via CD147 through novel modulation of lipid rafts.
J Immunol. 171 (4): 1707-14. -
Crisan, M. et al. (2008) A perivascular origin for mesenchymal stem cells in multiple human organs.
Cell Stem Cell. 3: 301-13. -
Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
PLoS Pathog. 2: e118. -
Apps, R. et al. (2011) Ex vivo functional responses to HLA-G differ between blood and decidual NK cells.
Mol Hum Reprod. 17: 577-86. -
Aspinall, A.I. et al. (2010) CX(3)CR1 and vascular adhesion protein-1-dependent recruitment of CD16(+) monocytes across human liver sinusoidal endothelium.
Hepatology. 51: 2030-9. -
Rodriguez JM et al. (2015) PyNTTTTGT and CpG Immunostimulatory Oligonucleotides: Effect on Granulocyte/Monocyte Colony-Stimulating Factor (GM-CSF) Secretion by Human CD56+ (NK and NKT) Cells.
PLoS One. 10 (2): e0117484.
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