CD56 antibody | MEM-188
FITC conjugated Mouse anti Human CD56 antibody, clone MEM-188 used for the identificationof CD56 positive cells from human peripheral blood mononuclear cell populations by flow cytometry.
Image caption:
Intracellular GM-CSF staining on CD56+ cells from hPBMC. (A) In the presence of the indicated ODN plus IL2: GM-CSF content in the cell culture without ODN stimulation was used as a control. Data are shown as mean +SD and are representative of three similar experiments. A specific response was defined as a response where the percentage of GM-CSF in CD56+ lymphocytes was at least 2-fold below the background observed in the medium control. The asterisk indicates statistically significant differences (p<0.05). (B) In the presence of IL2 in both CD3+ (NKT) and CD3- (NK) CD56+ dim or bright populations: ODNs and/or IL2 were added to the medium to reach a final concentration of 6 μg/ml and 400 IU/ml respectively. Dot plots are representative of three similar experiments.
From: Rodriguez JM, Marchicio J, López M, Ziblat A, Elias F, et al. (2015) PyNTTTTGT and CpG Immunostimulatory Oligonucleotides: Effect on Granulocyte/Monocyte Colony-Stimulating Factor (GM-CSF) Secretion by Human CD56+ (NK and NKT) Cells. PLoS ONE 10(2): e0117484.
AlexaFluor-647 conjugated Mouse anti Human CD56 antibody used for detection of CD56 on peripheral blood mononuclear cells by flow cytoimetry.
Image caption:
HLA-DR+ Accessory Cells Provide Activating Signals to NK Cells PBMCs from malaria-naïve donors were depleted of different populations of accessory cells, and NK cell responses to iRBCs were analysed after 24 h of co-culture. (A) Representative example of NK cell IFN-γ responses in undepleted PBMCs (top left), PBMCs depleted of all HLA-DR+ antigen-presenting cells (APC) (top right), CD14+ monocyte–depleted PBMCs (middle left), CD19+ B cell–depleted PBMCs (middle right), CD1c+ mDC–depleted PBMCs (bottom left), and BDCA-4+ pDC–depleted PBMCs (bottom right). FACS plots are gated on CD3− CD56+ lymphocytes. Percentages indicate the proportion of CD3− CD56+ NK cells that were positive for IFN-γ; data are based on the collection of 100,000 total events. (B) NK cell IFN-γ production after depletion of different antigen-presenting cell populations. Percentage of IFN-γ NK cells relative to the response in undepleted PBMCs is shown; each spot represents data for a different donor. *, p < 0.05; ***, p < 0.0001; p–values are for paired t tests of the difference between depleted and undepleted cells:
From: Newman KC, Korbel DS, Hafalla JC, Riley EM (2006) Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-γ Responses to Malaria. PLoS Pathog 2(12): e118.
AlexaFluor-647 conjugated Mouse anti Human CD56 antibody used for detection of CD56 on peripheral blood mononuclear cells by flow cytoimetry.
Image caption:
NK Cells Do Not Respond to iRBCs in the Absence of Accessory Cells PBMCs or MACS-purified NK cells from malaria-naïve donors were cultured alone or with IL-12 and IL-18, uRBCs, or live iRBCs for 24 h. Intracellular IFN-γ and surface expression of CD69 on CD3− CD56+ NK cells were analysed by flow cytometry. (A and B) Representative example of NK responses to uRBCs, IL-12 and IL-18, and iRBCs in PBMCs (A) and purified NK cells (B). FACS plots are gated on CD3− CD56+ lymphocytes. Percentages indicate the proportion of CD3− CD56+ NK cells in each gate; data are based on the collection of 100,000 total events. (C and D) Comparison of NK responses to iRBCs in PBMCs and in purified NK cells. (C) NK cell IFN-γ production for six donors. (D) CD69 surface expression levels for seven donors. **, p < 0.01; ***, p < 0.001; p-values are for paired t tests comparing stimulated and unstimulated NK cells from the same donor, or PBMCs and pure NK cells from the same donor. (E) MACS-purified, CTG-labelled NK cells were added to autologous PBMCs. IFN-γ production in CTG+ (MACS-purified) and CTG− (“untouched”) NK cells was assessed by flow cytometry after 24 h of stimulation with iRBCs. A representative example of the software gating strategy to analyse IFN-γ responses of CTG-labelled NK cells is shown. FACS plots were gated on CD3− CD56+ NK cells within the lymphocyte population (first panel), and NK cells were analysed for CTG staining (second panel). CTG+ CD3− CD56+ NK cells (third panel) or CTG− CD3− CD56+ NK cells (fourth panel) were selected and analysed for intracellular IFN-γ production. Numbers indicate the proportion of IFN-γ+ NK cells.
From: Newman KC, Korbel DS, Hafalla JC, Riley EM (2006) Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-γ Responses to Malaria. PLoS Pathog 2(12): e118.
Mouse anti Human CD56 antibody, clone MEM-188 recognizes CD56 a 140kDa cell surface glycoprotein expressed primarily by natural killer cells and a subset of T cells in the peripheral blood.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P13591 Related reagents
- Entrez Gene
- NCAM1 Related reagents
- Regulatory
- For research purposes only
Applications of CD56 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/25 | 1/50 Pack Size: 0.1 mg 1/100 Pack Size: 0.2 mg |
|
| Immunohistology - Frozen 1 | 1/40 | ||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |||
| Western Blotting 2 | Non-reducing conditions |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2 MEM-188 recognizes CD56 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F |
Product Specific References
References for CD56 antibody
-
Costa, P.et al (1997) CD56 Workshop Panel Report.
In: Leucocyte typing VI. White cell differentiation antigens. Garland Publishing Inc. New York and London. 271-2. -
Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.
J Immunol. 166 (4): 2364-71. -
Staffler G et al. (2003) Selective inhibition of T cell activation via CD147 through novel modulation of lipid rafts.
J Immunol. 171 (4): 1707-14. -
Crisan, M. et al. (2008) A perivascular origin for mesenchymal stem cells in multiple human organs.
Cell Stem Cell. 3: 301-13. -
Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
PLoS Pathog. 2: e118. -
Apps, R. et al. (2011) Ex vivo functional responses to HLA-G differ between blood and decidual NK cells.
Mol Hum Reprod. 17: 577-86. -
Aspinall, A.I. et al. (2010) CX(3)CR1 and vascular adhesion protein-1-dependent recruitment of CD16(+) monocytes across human liver sinusoidal endothelium.
Hepatology. 51: 2030-9. -
Rodriguez JM et al. (2015) PyNTTTTGT and CpG Immunostimulatory Oligonucleotides: Effect on Granulocyte/Monocyte Colony-Stimulating Factor (GM-CSF) Secretion by Human CD56+ (NK and NKT) Cells.
PLoS One. 10 (2): e0117484.
