Mouse anti Human CD56 antibody, clone MEM-188 recognizes CD56 a 140kDa cell surface glycoprotein expressed primarily by natural killer cells and a subset of T cells in the peripheral blood.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD56 antibody
||1/50 Pack Size: 0.1 mg
1/100 Pack Size: 0.2 mg
|Immunohistology - Frozen 1
|Immunohistology - Paraffin
|Western Blotting 2
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2 MEM-188 recognizes CD56 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD56 antibody
Costa, P.et al (1997) CD56 Workshop Panel Report.
In: Leucocyte typing VI. White cell differentiation antigens. Garland Publishing Inc. New York and London. 271-2.
Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.
J Immunol. 166 (4): 2364-71.
Staffler G et al. (2003) Selective inhibition of T cell activation via CD147 through novel modulation of lipid rafts.
J Immunol. 171 (4): 1707-14.
Crisan, M. et al. (2008) A perivascular origin for mesenchymal stem cells in multiple human organs.
Cell Stem Cell. 3: 301-13.
Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
PLoS Pathog. 2: e118.
Apps, R. et al. (2011) Ex vivo functional responses to HLA-G differ between blood and decidual NK cells.
Mol Hum Reprod. 17: 577-86.
Aspinall, A.I. et al. (2010) CX(3)CR1 and vascular adhesion protein-1-dependent recruitment of CD16(+) monocytes across human liver sinusoidal endothelium.
Hepatology. 51: 2030-9.
Rodriguez JM et al. (2015) PyNTTTTGT and CpG Immunostimulatory Oligonucleotides: Effect on Granulocyte/Monocyte Colony-Stimulating Factor (GM-CSF) Secretion by Human CD56+ (NK and NKT) Cells.
PLoS One. 10 (2): e0117484.