CD55 antibody | BRIC216
Mouse anti Human CD55
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD55 antibody, clone BRIC216 recognizes the CD55 antigen, a ~70 kDa glycoprotein also known as Decay Accelerating Factor (DAF). CD55 is distributed on erythrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. CD55 is also expressed at the foetal-maternal interfaces in placenta.CD55 has reduced expression on individuals with paroxysmal nocturnal haemoglobinuria. Mouse anti Human CD55 antibody, clone BRIC216 has a functional binding affinity to erythrocytes of 8.7 x 107 M-1. The antigen is pronase and trypsin resistant and chymotrypsin sensitive. Mouse anti Human CD55 antibody, clone BRIC216 recognizes the consensus region 3 of the DAF molecule, which contains the functional site, and the antibody blocks the function of DAF.|
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Human fibroblast cell line.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Functional Assays 1|
|Western Blotting 2|
- 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2Non-reducing conditions required.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Coyne, K.E. et al. (1992) Mapping of epitopes, glycosylation sites, and complement regulatory domains in human decay accelerating factor.
J Immunol. 149 (9): 2906-13.
References for CD55 antibody
Fodor, W.L. et al. (1995) A novel bifunctional chimeric complement inhibitor that regulates C3 convertase and formation of the membrane attack complex.
J Immunol. 155 (9): 4135-8.
Wiesner, J. et al. (1997) Host cell factor CD59 restricts complement lysis of Plasmodium falciparum-infected erythrocytes.
Eur J Immunol. 27 (10): 2708-13.
Triantafilou, M. et al. (2000) A 70 kDa MHC class I associated protein (MAP-70) identified as a receptor molecule for Coxsackievirus A9 cell attachment.
Hum Immunol. 61 (9): 867-78.
Tieng, V. et al. (2002) Binding of Escherichia coli adhesin AfaE to CD55 triggers cell-surface expression of the MHC class I-related molecule MICA.
Proc Natl Acad Sci U S A. 99: 2977-82.
Loberg, R.D. et al. (2006) Inhibition of decay-accelerating factor (CD55) attenuates prostate cancer growth and survival in vivo.
Neoplasia. 8: 69-78.
Wu, G. et al. (2007) Coagulation cascade activation triggers early failure of pig hearts expressing human complement regulatory genes.
Xenotransplantation. 14 (1): 34-47.
Kim, M.S. & Racaniello, V.R. (2007) Enterovirus 70 receptor utilization is controlled by capsid residues that also regulate host range and cytopathogenicity.
J Virol. 81 (16): 8648-55.
Ellison, B.S. et al. (2007) Complement susceptibility in glutamine deprived breast cancer cells.
Cell Div. 2: 20.
View The Latest Product References
Liszewski, M.K. et al. (2007) Modeling how CD46 deficiency predisposes to atypical hemolytic uremic syndrome.
Mol Immunol. 44: 1559-68.
Koch, N. et al. (2009) IL-10 protects monocytes and macrophages from complement-mediated lysis.
J Leukoc Biol. 86 (1): 155-66.
Tu, C.F. et al. (2010) The in vitro protection of human decay accelerating factor and hDAF/heme oxygenase-1 transgenes in porcine aortic endothelial cells against sera of Formosan macaques.
Transplant Proc. 42 (6): 2138-41.
Pahwa, R. et al. (2016) Modulation of PBMC-decay accelerating factor (PBMC-DAF) and cytokines in rheumatoid arthritis.
Mol Cell Biochem. 414 (1-2): 85-94.
Gullipalli, D. et al. (2018) Antibody Inhibition of Properdin Prevents Complement-Mediated Intravascular and Extravascular Hemolysis.
J Immunol. 201 (3): 1021-9.
Noda, G.S. et al. (2020) Specificities and isotypes of erythrocytes autoantibodies in patients with warm autoimmune hemolytic anemia
Rev Cubana Hematol Inmunol Hemoter 36(4): e1283
- Entrez Gene
- GO Terms
- GO:0005576 extracellular region
- GO:0005887 integral to plasma membrane
- GO:0005625 soluble fraction
- GO:0006958 complement activation, classical pathway
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0031225 anchored to membrane
- GO:0045087 innate immune response
- GO:0045121 membrane raft
- GO:0045730 respiratory burst
- View More GO Terms
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