CD55 antibody | BRIC216

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Mouse anti Human CD55

Product Type: Monoclonal Antibody
Clone: BRIC216
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA914T	F FN* IP WB*			25 µg
MCA914	F FN* IP WB*			0.2 mg

Mouse anti Human CD55 antibody, clone BRIC216 recognizes the CD55 antigen, a ~70 kDa glycoprotein also known as Decay Accelerating Factor (DAF). CD55 is distributed on erythrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. CD55 is also expressed at the foetal-maternal interfaces in placenta.CD55 has reduced expression on individuals with paroxysmal nocturnal haemoglobinuria. Mouse anti Human CD55 antibody, clone BRIC216 has a functional binding affinity to erythrocytes of 8.7 x 10⁷ M^-1. The antigen is pronase and trypsin resistant and chymotrypsin sensitive. Mouse anti Human CD55 antibody, clone BRIC216 recognises the consensus region 3 of the DAF molecule, which contains the functional site, and the antibody blocks the function of DAF.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

TRIS buffered saline.

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Human fibroblast cell line.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P08174 Related reagents
Entrez Gene: CD55 Related reagents
GO Terms: GO:0005576 extracellular region; GO:0005887 integral to plasma membrane; GO:0005625 soluble fraction; GO:0006958 complement activation, classical pathway; GO:0007204 elevation of cytosolic calcium ion concentration; GO:0031225 anchored to membrane; GO:0045087 innate immune response; GO:0045121 membrane raft; GO:0045730 respiratory burst
Regulatory: For research purposes only

Applications of CD55 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry
Functional Assays ¹
Immunoprecipitation
Western Blotting ²

¹This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
² Clone BRIC216 recognises human CD55 under non-reducing conditions.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Flow Cytometry

Functional Assays

Western Blotting

Product Specific References

References for CD55 antibody

Coyne, K.E. et al. (1992) Mapping of epitopes, glycosylation sites, and complement regulatory domains in human decay accelerating factor.
J Immunol. 149 (9): 2906-13.
Loberg, R.D. et al. (2006) Inhibition of decay-accelerating factor (CD55) attenuates prostate cancer growth and survival in vivo.
Neoplasia. 8: 69-78.
Triantafilou, M. et al. (2000) A 70 kDa MHC class I associated protein (MAP-70) identified as a receptor molecule for Coxsackievirus A9 cell attachment.
Hum Immunol. 61 (9): 867-78.
Ellison, B.S. et al. (2007) Complement susceptibility in glutamine deprived breast cancer cells.
Cell Div. 2: 20.
Tieng, V. et al. (2002) Binding of Escherichia coli adhesin AfaE to CD55 triggers cell-surface expression of the MHC class I-related molecule MICA.
Proc Natl Acad Sci U S A. 99: 2977-82.
Pahwa, R. et al. (2016) Modulation of PBMC-decay accelerating factor (PBMC-DAF) and cytokines in rheumatoid arthritis.
Mol Cell Biochem. 414 (1-2): 85-94.
Kim, M.S. & Racaniello, V.R. (2007) Enterovirus 70 receptor utilization is controlled by capsid residues that also regulate host range and cytopathogenicity.
J Virol. 81 (16): 8648-55.
Wu, G. et al. (2007) Coagulation cascade activation triggers early failure of pig hearts expressing human complement regulatory genes.
Xenotransplantation. 14 (1): 34-47.
Liszewski, M.K. et al. (2007) Modeling how CD46 deficiency predisposes to atypical hemolytic uremic syndrome.
Mol Immunol. 44: 1559-68.
Wiesner, J. et al. (1997) Host cell factor CD59 restricts complement lysis of Plasmodium falciparum-infected erythrocytes.
Eur J Immunol. 27 (10): 2708-13.
Fodor, W.L. et al. (1995) A novel bifunctional chimeric complement inhibitor that regulates C3 convertase and formation of the membrane attack complex.
J Immunol. 155 (9): 4135-8.
Tu, C.F. et al. (2010) The in vitro protection of human decay accelerating factor and hDAF/heme oxygenase-1 transgenes in porcine aortic endothelial cells against sera of Formosan macaques.
Transplant Proc. 42 (6): 2138-41.
Koch, N. et al. (2009) IL-10 protects monocytes and macrophages from complement-mediated lysis.
J Leukoc Biol. 86 (1): 155-66.
Kim, M.S. & Racaniello, V.R. (2007) Enterovirus 70 receptor utilization is controlled by capsid residues that also regulate host range and cytopathogenicity.
J Virol. 81 (16): 8648-55.

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