CD55 antibody | 67









Mouse anti Human CD55:Alexa Fluor® 488
Mouse anti Human CD55:Alexa Fluor® 647
Mouse anti Human CD55:FITC
Mouse anti Human CD55
Mouse anti Human CD55:RPE
Mouse anti Human CD55:RPE-Alexa Fluor® 647
Mouse anti Human CD55:Alexa Fluor® 700
- Product Type
- Monoclonal Antibody
- Clone
- 67
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1614A488 | F | 100 Tests/1ml |
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MCA1614A647 | F | 100 Tests/1ml |
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|
MCA1614F | F | 0.1 mg |
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|
MCA1614GA | C F WB | 0.1 mg |
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|
MCA1614 | C F WB | 0.2 mg |
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MCA1614PE | F | 100 Tests |
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MCA1614P647 | F | 100 Tests |
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MCA1614A700 | F | 100 Tests/1ml |
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CD55 is the complement regulatory protein, decay accelerating factor (DAF) (Lublin and Atkinson 1989). Human CD55 is a ~70 kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the
DAF has a wide distribution on cells in non-haematopoietic tissues, particularly epithelium and is found at the fetal-maternal interface in placenta (Holmes et al. 1990 and Yang et al. 2009). Soluble forms of DAF are found, for example, in plasma, saliva and urine (
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to RPE-Alexa Fluor 647 - lyophilized
- Product Form
- Purified IgG conjugated to Alexa Fluor® 700 - liquid
- Reconstitution
- Reconstitute with 1 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Carrier Free
- Yes
- Immunogen
- K562 cells
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P08174
- Entrez Gene
- CD55
- GO Terms
- GO:0005576 extracellular region
- GO:0005887 integral to plasma membrane
- GO:0005625 soluble fraction
- GO:0006958 complement activation, classical pathway
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0031225 anchored to membrane
- GO:0045087 innate immune response
- GO:0045121 membrane raft
- GO:0045730 respiratory burst
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD55 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | |||
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | ||
Flow Cytometry | 1/10 | 1/25 | |
Immunohistology - Frozen | 1/100 | 1/1000 | |
Western Blotting | |||
Flow Cytometry | Neat | 1/2 | |
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul. Please note: Bio-Rad do not recommend the use of this reagent to stain erythrocytes
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul. Please note: Bio-Rad do not recommend the use of this reagent to stain erythrocytes.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul. Please note: Bio-Rad do not recommend the use of this reagent to stain erythrocytes
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul. Please note: Bio-Rad do not recommend the use of this reagent to stain erythrocytes
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul. Please note: Bio-Rad do not recommend the use of this reagent to stain erythrocytes
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul. Please note: Bio-Rad do not recommend the use of this reagent to stain erythrocytes
- Histology Positive Control Tissue
- Human Tonsil
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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|
Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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|
Mouse IgG1 Negative Control:RPE-Alexa Fluor® 647 | MCA928P647 | F | 100 Tests/1ml |
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|
Mouse IgG1 Negative Control:Alexa Fluor® 700 | MCA928A700 | F | 100 Tests/1ml |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD55 antibody
-
Hadam, M.R. (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens.
Edited by Knapp, W. et al. Oxford University Press pp 694-697. -
Holmes, C.H. et al. (1990) Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy.
J Immunol. 144 (8): 3099-105. -
O'Brien, D.P. et al. (2009) Regulation of the Helicobacter pylori cellular receptor decay-accelerating factor.
J Biol Chem. 283: 23922-30. -
Yang, P. et al. (2009) Expression and modulation of RPE cell membrane complement regulatory proteins.
Invest Ophthalmol Vis Sci. 50: 3473-81. -
van de Sande, M.G. et al. (2011) Different stages of rheumatoid arthritis: features of the synovium in the preclinical phase.
Ann Rheum Dis. 70: 772-7. -
Mo, B. et al. (2006) ECC-1 cells: a well-differentiated steroid-responsive endometrial cell line with characteristics of luminal epithelium.
Biol Reprod. 75: 387-94. -
Araten, D.J. et al. (2005) A quantitative measurement of the human somatic mutation rate.
Cancer Res. 65: 8111-7. -
de Launay, D. et al. (2010) Silencing the expression of Ras family GTPase homologues decreases inflammation and joint destruction in experimental arthritis.
Am J Pathol. 177: 3010-24. -
Gheorghe, K.R. et al. (2011) Prostaglandin E2 synthesizing enzymes in rheumatoid arthritis B cells and the effects of B cell depleting therapy on enzyme expression.
PLoS One. ;6: e16378. -
Kraan, M.C. et al. (2004) T cells, fibroblast-like synoviocytes, and granzyme B+ cytotoxic cells are associated with joint damage in patients with recent onset rheumatoid arthritis.
Ann Rheum Dis. 63: 483-8. -
van Holten, J. et al. (2005) A multicentre, randomised, double blind, placebo controlled phase II study of subcutaneous interferon beta-1a in the treatment of patients with active rheumatoid arthritis.
Ann Rheum Dis. 64 (1): 64-9. -
Abreu, J.R. et al. (2009) The Ras guanine nucleotide exchange factor RasGRF1 promotes matrix metalloproteinase-3 production in rheumatoid arthritis synovial tissue.
Arthritis Res Ther.11(4):R121. -
Thurlings, R.M. et al. (2008) Synovial tissue response to rituximab: mechanism of action and identification of biomarkers of response.
Ann Rheum Dis. 67 (7): 917-25. -
Vos, K. et al. (2007) Early effects of rituximab on the synovial cell infiltrate in patients with rheumatoid arthritis.
Arthritis Rheum. 56 (3): 772-8. -
Edginton S et al. (2016) Effects of Rituximab and Infliximab Treatment on Carboxypeptidase B and Its Substrates in RA Synovium.
J Rheumatol. 43 (5): 846-54.
Further Reading
-
Lublin, D.M. & Atkinson, J.P. (1989) Decay-accelerating factor: biochemistry, molecular biology, and function.
Annu Rev Immunol. 7: 35-58. -
Daniels, G. (1989) Cromer-related antigens--blood group determinants on decay-accelerating factor.
Vox Sang. 56 (4): 205-11.
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