CD54 antibody | 84H10
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|Mouse anti Human CD54 antibody, clone 84H10 recognizes the D1 domain of ICAM-1.
It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Mouse anti Human CD54 antibody, clone 84H10 detects an antigen of ~90 kDa.
Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.
Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Dog
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- K562 cell line.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the MOPC 315 mouse myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Functional Assays 1|
|Immunohistology - Frozen 2||1/20||1/50|
|Immunohistology - Paraffin|
|Immunoprecipitation||5μg / 107 cells|
- 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD54 antibody
Makgoba, M.W. et al. (1988) ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells.
Nature. 331 (6151): 86-8.
Damle, N.K. et al. (1992) Intercellular adhesion molecule-2, a second counter-receptor for CD11a/CD18 (leukocyte function-associated antigen-1), provides a costimulatory signal for T-cell receptor-initiated activation of human T cells.
J Immunol. 148 (3): 665-71.
de Fougerolles, A.R. & Springer, T.A. (1992) Intercellular adhesion molecule 3, a third adhesion counter-receptor for lymphocyte function-associated molecule 1 on resting lymphocytes.
J Exp Med. 175 (1): 185-90.
Galkowska, H. et al. (1996) Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes.
Vet Immunol Immunopathol. 53 (3-4): 329-34.
Rothlein, R. et al. (1986) A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1.
J Immunol. 137 (4): 1270-4.
Dustin, M.L. et al. (1986) Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1).
J Immunol. 137 (1): 245-54.
Simmons, D. et al. (1988) ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM.
Nature. 331 (6157): 624-7.
Berendt, A.R. et al. (1992) The binding site on ICAM-1 for Plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1-binding site.
Cell. 68 (1): 71-81.
View The Latest Product References
Dyugovskaya, L. et al. (2002) Increased adhesion molecules expression and production of reactive oxygen species in leukocytes of sleep apnea patients.
Am J Respir Crit Care Med. 165: 934-9.
Bergmann-Leitner, E.S. et al. (2000) Differential role of Fas/Fas ligand interactions in cytolysis of primary and metastatic colon carcinoma cell lines by human antigen-specific CD8+ CTL.
J Immunol. 164: 4941-54.
Salvatierra, A. et al. (2001) Antithrombin III prevents early pulmonary dysfunction after lung transplantation in the dog.
Circulation. 104: 2975-80.
Jonsson, A.S. and Palmblad, J.E. (2001) Effects of ethanol on NF-kappaB activation, production of myeloid growth factors, and adhesive events in human endothelial cells.
J Infect Dis. 184: 761-9.
Chen, P.Y. et al. (2015) Endothelial-to-mesenchymal transition drives atherosclerosis progression.
J Clin Invest. 125 (12): 4514-28.
Salipante, S.J. et al. (2016) Recurrent somatic loss of TNFRSF14 in classical Hodgkin lymphoma.
Genes Chromosomes Cancer. 55 (3): 278-87.
- Entrez Gene
- GO Terms
- GO:0007157 heterophilic cell-cell adhesion
- GO:0001910 regulation of leukocyte mediated cytotoxicity
- GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
- GO:0002693 positive regulation of cellular extravasation
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005178 integrin binding
- GO:0005615 extracellular space
- GO:0007159 leukocyte cell-cell adhesion
- View More GO Terms
- GO:0022614 membrane to membrane docking
- GO:0046813 virion attachment, binding of host cell surface receptor
- GO:0050776 regulation of immune response
- GO:0050900 leukocyte migration
- GO:0051856 adhesion to symbiont
- GO:0060333 interferon-gamma-mediated signaling pathway
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