CD54 antibody | 84H10

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MCA532
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C * E F FN * IP 0.2 mg loader
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MCA532T
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Mouse anti Human CD54 antibody, clone 84H10 recognizes the D1 domain of ICAM-1.

It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Mouse anti Human CD54 antibody, clone 84H10 detects an antigen of ~90 kDa.

Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.

Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
K562 cell line.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the MOPC 315 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry Neat 1/10
Functional Assays 1
Immunohistology - Frozen 2 1/20 1/50
Immunohistology - Paraffin
Immunoprecipitation 5μg / 107 cells
  1. 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
  2. 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD54 antibody

  1. Makgoba, M.W. et al. (1988) ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells.
    Nature. 331 (6151): 86-8.
  2. Damle, N.K. et al. (1992) Intercellular adhesion molecule-2, a second counter-receptor for CD11a/CD18 (leukocyte function-associated antigen-1), provides a costimulatory signal for T-cell receptor-initiated activation of human T cells.
    J Immunol. 148 (3): 665-71.
  3. de Fougerolles, A.R. & Springer, T.A. (1992) Intercellular adhesion molecule 3, a third adhesion counter-receptor for lymphocyte function-associated molecule 1 on resting lymphocytes.
    J Exp Med. 175 (1): 185-90.
  4. Galkowska, H. et al. (1996) Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes.
    Vet Immunol Immunopathol. 53 (3-4): 329-34.
  5. Rothlein, R. et al. (1986) A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1.
    J Immunol. 137 (4): 1270-4.
  6. Dustin, M.L. et al. (1986) Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1).
    J Immunol. 137 (1): 245-54.
  7. Simmons, D. et al. (1988) ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM.
    Nature. 331 (6157): 624-7.
  8. Berendt, A.R. et al. (1992) The binding site on ICAM-1 for Plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1-binding site.
    Cell. 68 (1): 71-81.
  9. View The Latest Product References
  10. Dyugovskaya, L. et al. (2002) Increased adhesion molecules expression and production of reactive oxygen species in leukocytes of sleep apnea patients.
    Am J Respir Crit Care Med. 165: 934-9.
  11. Bergmann-Leitner, E.S. et al. (2000) Differential role of Fas/Fas ligand interactions in cytolysis of primary and metastatic colon carcinoma cell lines by human antigen-specific CD8+ CTL.
    J Immunol. 164: 4941-54.
  12. Salvatierra, A. et al. (2001) Antithrombin III prevents early pulmonary dysfunction after lung transplantation in the dog.
    Circulation. 104: 2975-80.
  13. Jonsson, A.S. and Palmblad, J.E. (2001) Effects of ethanol on NF-kappaB activation, production of myeloid growth factors, and adhesive events in human endothelial cells.
    J Infect Dis. 184: 761-9.
  14. Chen, P.Y. et al. (2015) Endothelial-to-mesenchymal transition drives atherosclerosis progression.
    J Clin Invest. 125 (12): 4514-28.
  15. Salipante, S.J. et al. (2016) Recurrent somatic loss of TNFRSF14 in classical Hodgkin lymphoma.
    Genes Chromosomes Cancer. 55 (3): 278-87.

Immunofluorescence

Synonyms
ICAM-1
RRID
AB_321784
UniProt
P05362
Entrez Gene
ICAM1
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0001910 regulation of leukocyte mediated cytotoxicity
GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
GO:0002693 positive regulation of cellular extravasation
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005178 integrin binding
GO:0005615 extracellular space
GO:0007159 leukocyte cell-cell adhesion
GO:0022614 membrane to membrane docking
GO:0046813 virion attachment, binding of host cell surface receptor
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration
GO:0051856 adhesion to symbiont
GO:0060333 interferon-gamma-mediated signaling pathway
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