CD54 antibody | 84H10

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F ResetMouse anti Human CD54
- Product Type
- Monoclonal Antibody
- Clone
- 84H10
- Isotype
- IgG1
- Specificity
- CD54
Mouse anti Human CD54 antibody, clone 84H10 recognizes the D1 domain of ICAM-1. It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Mouse anti Human CD54 antibody, clone 84H10 detects an antigen of ~90 kDa. Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1. Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- K562 cell line.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the MOPC 315 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
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Flow Cytometry | ![]() |
Neat | 1/10 |
Functional Assays 1 | ![]() |
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Immunohistology - Frozen 2 | ![]() |
1/20 | 1/50 |
Immunohistology - Paraffin | |||
Immunoprecipitation | ![]() |
5μg / 107 cells |
- 1 Removal of sodium azide is recommended prior to use in functional assays.
- 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control |
References for CD54 antibody
-
Makgoba, M.W. et al. (1988) ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells.
Nature. 331 (6151): 86-8. -
Simmons, D. et al. (1988) ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM.
Nature. 331 (6157): 624-7. -
Damle, N.K. et al. (1992) Intercellular adhesion molecule-2, a second counter-receptor for CD11a/CD18 (leukocyte function-associated antigen-1), provides a costimulatory signal for T-cell receptor-initiated activation of human T cells.
J Immunol. 148 (3): 665-71. -
Berendt, A.R. et al. (1992) The binding site on ICAM-1 for Plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1-binding site.
Cell. 68 (1): 71-81. -
Bergmann-Leitner, E.S. et al. (2000) Differential role of Fas/Fas ligand interactions in cytolysis of primary and metastatic colon carcinoma cell lines by human antigen-specific CD8+ CTL.
J Immunol. 164: 4941-54. -
Salvatierra, A. et al. (2001) Antithrombin III prevents early pulmonary dysfunction after lung transplantation in the dog.
Circulation. 104: 2975-80. -
Jonsson, A.S. and Palmblad, J.E. (2001) Effects of ethanol on NF-kappaB activation, production of myeloid growth factors, and adhesive events in human endothelial cells.
J Infect Dis. 184: 761-9. -
Dyugovskaya, L. et al. (2002) Increased adhesion molecules expression and production of reactive oxygen species in leukocytes of sleep apnea patients.
Am J Respir Crit Care Med. 165: 934-9.
View The Latest Product References
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Chen, P.Y. et al. (2015) Endothelial-to-mesenchymal transition drives atherosclerosis progression.
J Clin Invest. 125 (12): 4514-28. -
Salipante, S.J. et al. (2016) Recurrent somatic loss of TNFRSF14 in classical Hodgkin lymphoma.
Genes Chromosomes Cancer. 55 (3): 278-87. -
Connolly, D.M. et al. (2023) Early Human Pathophysiological Responses to Exertional Hypobaric Decompression Stress.
Aerosp Med Hum Perform. 94 (10): 738-749. -
Bertolini, M. et al. (2023) Mechanical epilation exerts complex biological effects on human hair follicles and perifollicular skin: An ex vivo study approach
Int J Cosmetic Sci. 03 Nov [Epub ahead of print].
Further Reading
-
Galkowska, H. et al. (1996) Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes.
Vet Immunol Immunopathol. 53 (3-4): 329-34.
- Synonyms
- ICAM-1
- RRID
- AB_321784
- UniProt
- P05362
- Entrez Gene
- ICAM1
- GO Terms
- GO:0007157 heterophilic cell-cell adhesion
- GO:0001910 regulation of leukocyte mediated cytotoxicity
- GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
- GO:0002693 positive regulation of cellular extravasation
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005178 integrin binding
- GO:0005615 extracellular space
- GO:0007159 leukocyte cell-cell adhesion
- View More GO Terms
- GO:0022614 membrane to membrane docking
- GO:0046813 virion attachment, binding of host cell surface receptor
- GO:0050776 regulation of immune response
- GO:0050900 leukocyte migration
- GO:0051856 adhesion to symbiont
- GO:0060333 interferon-gamma-mediated signaling pathway
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