CD54 antibody | 15.2

Product Code Applications Pack Size List Price Your Price Qty
MCA1615T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P 20 µg loader
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loader
MCA1615
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SDS Safety Datasheet SDS
C F IP P 0.2 mg loader
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loader
MCA1615GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P 0.1 mg loader
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loader
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Mouse anti Human CD54 antibody, clone 15.2 recognizes the human CD54 cell surface antigen also known as intracellular Adhesion Molecule -1 (ICAM-1) or Major group rhinovirus receptor.

CD54 is expressed by many cells following activation by inflammatory mediators. It is a 505 amino acid with an additional 27 amino acid signal peptide ~90 kDa single pass type I transmembrane glycoprotein bearing 5 Ig-like C2-type domains.

Mouse anti Human CD54 antibody, clone 15.2 blocks CD54 function (Berendt et al. 1992). Mouse anti Human CD54 antibody, clone 15.2 binds to an epitope on the N-terminal Ig-like domain within a region designated the L43 loop (Chakravorty and Craig 2005).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA1615T, MCA1615: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1615GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human monocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0-Ag14 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1/50 1/100
Immunohistology - Paraffin
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Human Tonsil

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Description Mouse IgG1 Negative Control

References for CD54 antibody

  1. Dransfield, I. et al. (1992) Interaction of leukocyte integrins with ligand is necessary but not sufficient for function.
    J Cell Biol. 116:1527-35.
  2. Berendt, A. et al. (1992) The binding site on ICAM-1 for plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1- binding site.
    Cell. 68: 71-81.
  3. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
    J Pharmacol Exp Ther. 321 (2): 656-62.
  4. Baratin, M. et al. (2007) Dissection of the role of PfEMP1 and ICAM-1 in the sensing of Plasmodium-falciparum-infected erythrocytes by natural killer cells.
    PLos One 2: e228.
  5. van Buul, J.D. et al. (2010) Inside-out regulation of ICAM-1 dynamics in TNF-alpha-activated endothelium.
    PLoS One 5: e11336.
  6. Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
    J Cell Physiol. 214: 75-83.
  7. Di Lorenzo, A. et al. (2011) Endothelial reticulon-4B (Nogo-B) regulates ICAM-1-mediated leukocyte transmigration and acute inflammation.
    Blood. 117: 2284-95.
  8. Porter, J.C. and Hall, A. (2009) Epithelial ICAM-1 and ICAM-2 regulate the egression of human T cells across the bronchial epithelium.
    FASEB J. 23: 492-502.
  9. View The Latest Product References
  10. Corvaisier, M. et al. (2005) V gamma 9V delta 2 T cell response to colon carcinoma cells.
    J Immunol. 175: 5481-8.
  11. Horrocks, P. et al. (2005) PfEMP1 expression is reduced on the surface of knobless Plasmodium falciparum infected erythrocytes.
    J Cell Sci. 118: 2507-18.
  12. Lozanoska-Ochser, B. et al. (2008) Expression of CD86 on human islet endothelial cells facilitates T cell adhesion and migration.
    J Immunol. 181: 6109-16.
  13. Norling, L.V. et al. (2008) Inhibitory control of endothelial galectin-1 on in vitro and in vivo lymphocyte trafficking.
    FASEB J. 22: 682-90.
  14. Baumer, Y. et al. (2011) Telomerase-based immortalization modifies the angiogenic/inflammatory responses of human coronary artery endothelial cells.
    Exp Biol Med (Maywood). 236: 692-700.
  15. Lask, A. et al. (2011) TCR-independent killing of B cell malignancies by anti-third-party CTLs: the critical role of MHC-CD8 engagement.
    J Immunol. 187 (4): 2006-14.
  16. Sommaggio, R. et al. (2012) Multiple Receptors Trigger Human NK Cell-Mediated Cytotoxicity against Porcine Chondrocytes.
    J Immunol. 188: 2075-83.
  17. Murphy, A.J. et al. (2013) Anti-inflammatory functions of apolipoprotein a-I and high-density lipoprotein are preserved in trimeric apolipoprotein a-I.
    J Pharmacol Exp Ther. 344: 41-9.
  18. Sumagin R et al. (2014) Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment.
    Mucosal Immunol. 7 (4): 905-15.
  19. Sugden SM et al. (2017) HIV-1 Vpu Downmodulates ICAM-1 Expression, Resulting in Decreased Killing of Infected CD4+ T Cells by NK Cells.
    J Virol. 91 (8): pii: e02442-16.
  20. Lennartz, F. et al. (2015) Mapping the Binding Site of a Cross-Reactive Plasmodium falciparum PfEMP1 Monoclonal Antibody Inhibitory of ICAM-1 Binding.
    J Immunol. 195 (7): 3273-83.
  21. Schoppmeyer, R. et al. (2022) The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner.
    Cell Rep. 38 (3): 110243.
  22. Grönloh, M.L.B. et al. (2022) Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1
    bioRxiv 17 Jan [Epub ahead of print].

Flow Cytometry

Immunofluorescence

Synonyms
ICAM-1
RRID
AB_321783
UniProt
P05362
Entrez Gene
ICAM1
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0001910 regulation of leukocyte mediated cytotoxicity
GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
GO:0002693 positive regulation of cellular extravasation
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005178 integrin binding
GO:0005615 extracellular space
GO:0007159 leukocyte cell-cell adhesion
GO:0022614 membrane to membrane docking
GO:0046813 virion attachment, binding of host cell surface receptor
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration
GO:0051856 adhesion to symbiont
GO:0060333 interferon-gamma-mediated signaling pathway
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