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CD53 antibody | MEM-53

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Anti Human CD53 Antibody, clone MEM-53 thumbnail image 1 Anti Human CD53 Antibody, clone MEM-53 thumbnail image 2 Anti Human CD53 Antibody, clone MEM-53 thumbnail image 3 Anti Human CD53 Antibody, clone MEM-53 thumbnail image 4 Anti Human CD53 Antibody, clone MEM-53 thumbnail image 5 Anti Human CD53 Antibody, clone MEM-53 thumbnail image 6
Anti Human CD53 Antibody, clone MEM-53 gallery image 1
Figure A. RPE-A647 tandem conjugated Mouse anti Human CD3 (MCA463P647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE-A647 tandem conjugated Mouse anti Human CD3 (MCA463P647) and FITC conjugated Mouse anti Human CD53 (MCA723F). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Anti Human CD53 Antibody, clone MEM-53 gallery image 2
Published customer image:
Mouse anti Human CD53 antibody, clone MEM-53 (MCA723G) used for the evaluation of CD53 expression on white blood cell subsets by flow cytometry.
Image caption:
Expression of CD37 and CD53 on immune cell subsets in blood. a Flow cytometry analysis of expression of CD37 or CD53 (black line) on CD4 and CD8 T cells, B cells, monocytes and NK cells versus isotype control (gray line). Gating strategy is presented in Supplementary Figure 1. Expression levels of CD37 (b) and CD53 (c) were normalized for isotype staining by background subtraction. Experiments were performed with PBLs from three healthy donors. Data present mean ± SD. d Flow cytometry analysis of expression of CD37 or CD53 (black line) on mDCs (BDCA1+CD19−) or pDCs (BDCA2+) versus isotype control (gray line). Expression levels of CD37 (e) and CD53 (f) were normalized for isotype staining by background subtraction. Experiments were performed with PBLs from two healthy donors. Data present mean ± SD

From: de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB. Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs. Histochem Cell Biol . 2015 Aug;144(2):133-46.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD53 Antibody, clone MEM-53 gallery image 3
Published customer image:
Mouse anti Human CD53 antibody, clone MEM-53 (MCA723G) used for the evaluation of CD53 distribution on monocytes using immunofluorescence.
Image caption:
Subcellular localization of CD37 and CD53. Localization of a CD37 or b CD53 (green) in monocytes was studied by dual staining with calreticulin (ER), syntaxin 13 (endosomes) or Lamp1 (lysosomes) (red). Merge: co-localization in yellow (white arrows). Scale bar 5 μm

From: de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB.
Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
Histochem Cell Biol. 2015 Aug;144(2):133-46.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD53 Antibody, clone MEM-53 gallery image 4
Published customer image:
Mouse anti Human CD53 antibody, clone MEM-53 (MCA723G) used for the evaluation of CD53 expression on erythroid cultures by flow cytometry.
Image caption:
Erythroid culture characterization and expression of tetraspanins and integrins during terminal maturation. A. Temporal expression of erythroid-specific markers, Kell, GPA and AE1 and morphology of the culture at the same time points. AE1 was tested from day 5 onwards. B. Tetraspanin and integrin profile of the same cultures as shown in A. Results are depicted from one culture where directly conjugated antibodies were used (days 3 and 4) and a second culture with indirectly labeled antibodies (day 5 onwards). The y-axis scale is linear to 350 counts; the x-axis is logarithmic to 104. Images were captured on a Leica DM750 microscope, x20 magnification, using Image-Pro Express 6.0 software.

From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, et al. (2013) Tetraspanins CD81 and CD82 Facilitate α4β1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1.
PLoS ONE 8(5): e62654.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD53 Antibody, clone MEM-53 gallery image 5
Published customer image:
Mouse anti Human CD53 antibody, clone MEM-53 (MCA723G) used for the evaluation of CD53 expression on erythroid cultures by immunoprecipitation.
Image caption:
Several anti-tetraspanin antibodies co-precipitate β1 integrins from HEL cells solubilised in Brij-97. Precipitates were prepared from HEL cells solubilised in different detergents in the presence of Mn2+. CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; α4, HP2/1; αL, TS1/22; αIIb, PAB-1. Precipitates were run on 7.5% non-reduced gels.

From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, et al. (2013) Tetraspanins CD81 and CD82 Facilitate α4β1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1.
PLoS ONE 8(5): e62654.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD53 Antibody, clone MEM-53 gallery image 6
Published customer image:
Mouse anti Human CD53 antibody, clone MEM-53 (MCA723) used for the evaluation of CD53 expression on erythroid cultures by immunoprecipitation and western blotting.
Image caption:
Co-precipitation of β1 and β3 integrins by tetraspanins with different cations from HEL cells. Precipitates were prepared from HEL cells solubilised in Brij-97 in the presence of EDTA or cations. β1 is co-precipitated by CD63, CD81, CD82 and CD151 while β3 is co-precipitated by all tetraspanins in the presence of Mn2+. CD151 co-precipitates β1 under all conditions. Integrins were separated on 7.5% gels, tetraspanin controls on 12% gels, both non-reducing conditions. CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; α4, HP2/1; αL, TS1/22; αIIb, PAB-1.

From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, et al. (2013) Tetraspanins CD81 and CD82 Facilitate α4β1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1.
PLoS ONE 8(5): e62654.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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F IF IP WB

Mouse anti Human CD53:FITC

Product Type
Monoclonal Antibody
Clone
MEM-53
Isotype
IgG1
Specificity
CD53

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Product Code Applications Pack Size List Price Your Price Qty
MCA723F
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F 100 Tests loader
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Mouse anti Human CD53 antibody, clone MEM-53 recognizes the human CD53 cell surface antigen, also known as Tetraspanin-25. CD53 is a 219 amino acid multi pass transmembrane glycoprotein of 32-42 kDa. CD53 is expressed by all leucocytes, but is absent from erythrocytes and platelets.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for CD53 antibody

  1. Dunlock, V.E. et al. (2019) Dynamic regulation of CD45 by tetraspanin CD53
    bioRxiv Nov 29 [Epub ahead of print].
  2. Bazil, V. et al. (1989) Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.
    Folia Biol (Praha). 35 (5): 289-97.
  3. Angelisová P et al. (1990) The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens.
    Immunogenetics. 32 (4): 281-5.
  4. Mollinedo, F. et al. (1997) Recurrent infectious diseases in human CD53 deficiency.
    Clin Diagn Lab Immunol. 4: 229-31.
  5. de Winde, C.M. et al. (2015) Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
    Histochem Cell Biol. 144 (2): 133-46.
  6. Matsumoto K et al. (1999) Functional expression of transmembrane 4 superfamily molecules on human eosinophils.
    Int Arch Allergy Immunol. 120 Suppl 1: 38-44.
  7. Spring, F.A. et al. (2013) Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1.
    PLoS One. 8 (5): e62654.
  8. Tippett, E. et al. (2013) Characterization of tetraspanins CD9, CD53, CD63, and CD81 in monocytes and macrophages in HIV-1 infection.
    J Leukoc Biol. 93 (6): 913-20.
    9. View The Latest Product References
  9. Zuidscherwoude, M. et al. (2015) The tetraspanin web revisited by super-resolution microscopy.
    Sci Rep. 5: 12201.

RRID
AB_321780
UniProt
P19397
Entrez Gene
CD53
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0007165 signal transduction

MCA723F

151251

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