CD53 antibody | MEM-53
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|Mouse anti Human CD53 antibody, clone MEM-53 recognizes the human CD53 cell surface antigen, also known as Tetraspanin-25. CD53 is a 219 amino acid multi pass transmembrane glycoprotein of 32-42 kDa. CD53 is expressed by all leucocytes, but is absent from erythrocytes and platelets.|
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Western Blotting 1|
- 1Clone MEM-53 recognizes human CD53 under non-reducing conditions.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD53 antibody
Dunlock, V.E. et al. (2019) Dynamic regulation of CD45 by tetraspanin CD53
bioRxiv Nov 29 [Epub ahead of print].
Bazil, V. et al. (1989) Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.
Folia Biol (Praha). 35 (5): 289-97.
Angelisová P et al. (1990) The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens.
Immunogenetics. 32 (4): 281-5.
Mollinedo, F. et al. (1997) Recurrent infectious diseases in human CD53 deficiency.
Clin Diagn Lab Immunol. 4: 229-31.
de Winde, C.M. et al. (2015) Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
Histochem Cell Biol. 144 (2): 133-46.
Matsumoto K et al. (1999) Functional expression of transmembrane 4 superfamily molecules on human eosinophils.
Int Arch Allergy Immunol. 120 Suppl 1: 38-44.
Spring, F.A. et al. (2013) Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1.
PLoS One. 8 (5): e62654.
Tippett, E. et al. (2013) Characterization of tetraspanins CD9, CD53, CD63, and CD81 in monocytes and macrophages in HIV-1 infection.
J Leukoc Biol. 93 (6): 913-20.
View The Latest Product References
Zuidscherwoude, M. et al. (2015) The tetraspanin web revisited by super-resolution microscopy.
Sci Rep. 5: 12201.
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