CD53 antibody | MEM-53
Mouse anti Human CD53 antibody, clone MEM-53 (MCA723G) used for the evaluation of CD53 expression on white blood cell subsets by flow cytometry.
Image caption:
Expression of CD37 and CD53 on immune cell subsets in blood. a Flow cytometry analysis of expression of CD37 or CD53 (black line) on CD4 and CD8 T cells, B cells, monocytes and NK cells versus isotype control (gray line). Gating strategy is presented in Supplementary Figure 1. Expression levels of CD37 (b) and CD53 (c) were normalized for isotype staining by background subtraction. Experiments were performed with PBLs from three healthy donors. Data present mean ± SD. d Flow cytometry analysis of expression of CD37 or CD53 (black line) on mDCs (BDCA1+CD19−) or pDCs (BDCA2+) versus isotype control (gray line). Expression levels of CD37 (e) and CD53 (f) were normalized for isotype staining by background subtraction. Experiments were performed with PBLs from two healthy donors. Data present mean ± SD
From: de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB. Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs. Histochem Cell Biol. 2015 May 8. [Epub ahead of print]
Mouse anti Human CD53 antibody, clone MEM-53 (MCA723G) used for the evaluation of CD53 distribution on monocytes using immunofluorescence.
Image caption:
Subcellular localization of CD37 and CD53. Localization of a CD37 or b CD53 (green) in monocytes was studied by dual staining with calreticulin (ER), syntaxin 13 (endosomes) or Lamp1 (lysosomes) (red). Merge: co-localization in yellow (white arrows). Scale bar 5 μm
From: de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB.
Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
Histochem Cell Biol. 2015 May 8. [Epub ahead of print]
Mouse anti Human CD53 antibody, clone MEM-53 (MCA723G) used for the evaluation of CD53 expression on erythroid cultures by flow cytometry.
Image caption:
Erythroid culture characterization and expression of tetraspanins and integrins during terminal maturation. A. Temporal expression of erythroid-specific markers, Kell, GPA and AE1 and morphology of the culture at the same time points. AE1 was tested from day 5 onwards. B. Tetraspanin and integrin profile of the same cultures as shown in A. Results are depicted from one culture where directly conjugated antibodies were used (days 3 and 4) and a second culture with indirectly labeled antibodies (day 5 onwards). The y-axis scale is linear to 350 counts; the x-axis is logarithmic to 104. Images were captured on a Leica DM750 microscope, x20 magnification, using Image-Pro Express 6.0 software.
From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, et al. (2013) Tetraspanins CD81 and CD82 Facilitate α4β1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1.
PLoS ONE 8(5): e62654.
Mouse anti Human CD53 antibody, clone MEM-53 (MCA723G) used for the evaluation of CD53 expression on erythroid cultures by immunoprecipitation.
Image caption:
Several anti-tetraspanin antibodies co-precipitate β1 integrins from HEL cells solubilised in Brij-97. Precipitates were prepared from HEL cells solubilised in different detergents in the presence of Mn2+. CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; α4, HP2/1; αL, TS1/22; αIIb, PAB-1. Precipitates were run on 7.5% non-reduced gels.
From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, et al. (2013) Tetraspanins CD81 and CD82 Facilitate α4β1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1.
PLoS ONE 8(5): e62654.
Mouse anti Human CD53 antibody, clone MEM-53 (MCA723) used for the evaluation of CD53 expression on erythroid cultures by immunoprecipitation and western blotting.
Image caption:
Co-precipitation of β1 and β3 integrins by tetraspanins with different cations from HEL cells. Precipitates were prepared from HEL cells solubilised in Brij-97 in the presence of EDTA or cations. β1 is co-precipitated by CD63, CD81, CD82 and CD151 while β3 is co-precipitated by all tetraspanins in the presence of Mn2+. CD151 co-precipitates β1 under all conditions. Integrins were separated on 7.5% gels, tetraspanin controls on 12% gels, both non-reducing conditions. CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; α4, HP2/1; αL, TS1/22; αIIb, PAB-1.
From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, et al. (2013) Tetraspanins CD81 and CD82 Facilitate α4β1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1.
PLoS ONE 8(5): e62654.
Mouse anti Human CD53 antibody, clone MEM-53 recognizes the human CD53 cell surface antigen, also known as Tetraspanin-25. CD53 is a 219 amino acid multi pass transmembrane glycoprotein of 32-42 kDa. CD53 is expressed by all leucocytes, but is absent from erythrocytes and platelets.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19397
- Entrez Gene
- CD53
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0007165 signal transduction
- Regulatory
- For research purposes only
Applications of CD53 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/10 | 1/25 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting 1 |
- 1Clone MEM-53 recognizes human CD53 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD53 antibody
-
Bazil, V. et al. (1989) Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.
Folia Biol (Praha). 35 (5): 289-97. -
Angelisová P et al. (1990) The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens.
Immunogenetics. 32 (4): 281-5. -
Mollinedo, F. et al. (1997) Recurrent infectious diseases in human CD53 deficiency.
Clin Diagn Lab Immunol. 4: 229-31. -
de Winde, C.M. et al. (2015) Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
Histochem Cell Biol. 144 (2): 133-46. -
Matsumoto K et al. (1999) Functional expression of transmembrane 4 superfamily molecules on human eosinophils.
Int Arch Allergy Immunol. 120 Suppl 1: 38-44. -
Spring, F.A. et al. (2013) Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1.
PLoS One. 8 (5): e62654. -
Tippett, E. et al. (2013) Characterization of tetraspanins CD9, CD53, CD63, and CD81 in monocytes and macrophages in HIV-1 infection.
J Leukoc Biol. 93 (6): 913-20. -
Zuidscherwoude, M. et al. (2015) The tetraspanin web revisited by super-resolution microscopy.
Sci Rep. 5: 12201.
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