CD53 antibody | MEM-53

Mouse anti Human CD53

Product Type
Monoclonal Antibody
Clone
MEM-53
Isotype
IgG1
Specificity
CD53

Product Code Applications Pack Size List Price Your Price Qty
MCA723G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP WB * 0.2 mg loader
List Price Your Price
loader
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Mouse anti Human CD53 antibody, clone MEM-53 recognizes the human CD53 cell surface antigen, also known as Tetraspanin-25. CD53 is a 219 amino acid multi pass transmembrane glycoprotein of 32-42 kDa. CD53 is expressed by all leucocytes, but is absent from erythrocytes and platelets.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/25
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 1
  1. 1Clone MEM-53 recognizes human CD53 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD53 antibody

  1. Dunlock, V.E. et al. (2019) Dynamic regulation of CD45 by tetraspanin CD53
    bioRxiv Nov 29 [Epub ahead of print].
  2. Bazil, V. et al. (1989) Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.
    Folia Biol (Praha). 35 (5): 289-97.
  3. Angelisová P et al. (1990) The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens.
    Immunogenetics. 32 (4): 281-5.
  4. Mollinedo, F. et al. (1997) Recurrent infectious diseases in human CD53 deficiency.
    Clin Diagn Lab Immunol. 4: 229-31.
  5. de Winde, C.M. et al. (2015) Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
    Histochem Cell Biol. 144 (2): 133-46.
  6. Matsumoto K et al. (1999) Functional expression of transmembrane 4 superfamily molecules on human eosinophils.
    Int Arch Allergy Immunol. 120 Suppl 1: 38-44.
  7. Spring, F.A. et al. (2013) Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1.
    PLoS One. 8 (5): e62654.
  8. Tippett, E. et al. (2013) Characterization of tetraspanins CD9, CD53, CD63, and CD81 in monocytes and macrophages in HIV-1 infection.
    J Leukoc Biol. 93 (6): 913-20.
  9. View The Latest Product References
  10. Zuidscherwoude, M. et al. (2015) The tetraspanin web revisited by super-resolution microscopy.
    Sci Rep. 5: 12201.

RRID
AB_2075724
UniProt
P19397
Entrez Gene
CD53
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0007165 signal transduction
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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