CD51/CD61 antibody | 23C6
Mouse anti Human CD51/CD61 antibody, clone 23C6 recognizes the intact complex formed between the CD51 and CD61 molecules (alpha V and beta 3 integrins). This complex binds vitronectin at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Mouse anti Human CD51/CD61 antibody, clone 23C6 reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Bovine Pig Chicken Rabbit
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Osteoclasts from osteoclastomas.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse X63.Ag8.653 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch
- P06756 Related reagents
- P05106 Related reagents
- Entrez Gene
- ITGB3 Related reagents
- ITGAV Related reagents
- GO Terms
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0002576 platelet degranulation
- GO:0050839 cell adhesion molecule binding
- GO:0004872 receptor activity
- GO:0005161 platelet-derived growth factor receptor binding
- GO:0008305 integrin complex
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007411 axon guidance
- GO:0042802 identical protein binding
- GO:0010595 positive regulation of endothelial cell migration
- GO:0010745 negative regulation of macrophage derived foam cell differentiation
- GO:0010888 negative regulation of lipid storage
- GO:0014909 smooth muscle cell migration
- GO:0030168 platelet activation
- GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
- GO:0031092 platelet alpha granule membrane
- GO:0032147 activation of protein kinase activity
- GO:0032369 negative regulation of lipid transport
- GO:0035295 tube development
- GO:0043184 vascular endothelial growth factor receptor 2 binding
- GO:0044419 interspecies interaction between organisms
- GO:0045124 regulation of bone resorption
- GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050748 negative regulation of lipoprotein metabolic process
- GO:0050900 leukocyte migration
- GO:0060055 angiogenesis involved in wound healing
- GO:0071062 alphav-beta3 integrin-vitronectin complex
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- For research purposes only
Applications of CD51/CD61 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1|
|Immunohistology - Paraffin|
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Mouse IgG1 Negative Control||MCA928||100 Tests||F|
Product Specific References
References for CD51/CD61 antibody
Horton, M.A. et al. (1985) Monoclonal antibodies to osteoclastomas (giant cell bone tumors): definition of osteoclast-specific cellular antigens.
Cancer Res. 45 (11 Pt 2): 5663-9.
Davies, J. et al. (1989) The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor.
J Cell Biol. 109 (4 Pt 1): 1817-26.
Simpson, A. & Horton, M.A. (1989) Expression of the vitronectin receptor during embryonic development; an immunohistological study of the ontogeny of the osteoclast in the rabbit.
Br J Exp Pathol. 70 (3): 257-65.
Viertlboeck, B.C, and Göbel, T.W. (2007) Chicken thrombocytes express the CD51/CD61 integrin.
Vet Immunol Immunopathol. 119:137-41.
Danks, L. et al. (2002) Synovial macrophage-osteoclast differentiation in inflammatory arthritis.
Ann Rheum Dis. 61: 916-21.
Knowles, H.J. et al. (2010) Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4.
FASEB J. 24: 4648-59.
Lau, Y.S. et al. (2007) Cellular and humoral mechanisms of osteoclast formation in Ewing's sarcoma
Br J Cancer. 96: 1716-22.
Lau, Y.S. et al. (2006) Malignant melanoma and bone resorption.
Br J Cancer. 94: 1496-503.
Mabilleau, G. et al. (2009) Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
PLoS One. 4:e4173.
Whyte, L.S. et al. (2009) The putative cannabinoid receptor GPR55 affects osteoclast function in vitro and bone mass in vivo.
Proc Natl Acad Sci U S A. 106: 16511-6.
Zhao W et al. (2015) The Gametocytes of Leucocytozoon sabrazesi Infect Chicken Thrombocytes, Not Other Blood Cells.
PLoS One. 10 (7): e0133478.
Iseri, V.J. & Klasing, K.C. (2013) Dynamics of the systemic components of the chicken (Gallus gallus domesticus) immune system following activation by Escherichia coli; implications for the costs of immunity.
Dev Comp Immunol. 40 (3-4): 248-57.
Knowles, H.J. (2017) Hypoxia-Induced Fibroblast Growth Factor 11 Stimulates Osteoclast-Mediated Resorption of Bone.
Calcif Tissue Int. 100 (4): 382-91.