CD51/CD61 antibody | 23C6
Mouse anti CD51/CD61 antibody, clone 23C6 (MCA757G) used for identifying vitronectin receptor expression by immunocytochemistry.
Image caption:
IL-32-treatment induced a dose dependent differentiation of osteoclast precursors.
(A) TRAcP and VNR positive staining of newly-formed multinucleated cells differentiated from human PBMCs in the presence of 25 ng/ml of M-CSF±100 ng/ml of IL-32. Black bar represents 50 μm. (B) The number of newly-generated TRAcP positive multinucleated cells is dependent on the concentration of IL-32. (C) Size of newly-generated multinucleated cells is not increased at doses higher than 50 ng/ml of IL-32. (D) The number of nuclei per newly-generated multinucleated cells is significantly increased in a dose-dependent manner with the optimal response at 100 ng/ml of IL-32. P values represent the statistical significances between each group using Mann-Whitney test.
From: Insert article citation with space after colon and Hyperlink to PubMed record.Citation: Mabilleau G, Sabokbar A (2009)
Interleukin-32 Promotes Osteoclast Differentiation but Not Osteoclast Activation.
PLoS ONE 4(1): e4173.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD51/CD61 antibody, clone 23C6 (MCA757G) used for the identification of chicken thrombocytes by immunofluorescence.
Image caption:
Images of infected chicken blood cells that are stained with anti-CD51/CD61 monoclonal antibody and observed under a regular fluorescent microscope. (A-D) stained cells with characteristic of thrombocytes having round nuclei and small pale cytoplasm (green arrows) and a weakly stained parasite-infected cell (white arrows). (E-H) A cluster of five thrombocytes stained red by the anti-CD51/CD61 antibody. (I-L) A stained parasite-infected cell and a stained thrombocyte (small red dot). (M-P) Another stained parasite-infected cell (red). Inset images of enlarged gametocytes in L and P show host cell membrane (arrows) and nuclei of host cells (marked ‘N’). Image A-D are from formaldehyde fixed cells; images E-P are from ‘wet’ IFA without fixation. The white scale bar is 10 μM.
From: Zhao W, Liu J, Xu R, Zhang C, Pang Q, Chen X, et al. (2015)
The Gametocytes of
Leucocytozoon sabrazesi Infect Chicken Thrombocytes, Not Other Blood Cells.
PLoS ONE 10(7): e0133478.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD51/CD61 antibody, clone 23C6 recognizes the intact complex formed between the CD51 and CD61 molecules (alpha V and beta 3 integrins). This complex binds vitronectin at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Mouse anti Human CD51/CD61 antibody, clone 23C6 reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Pig Chicken Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Osteoclasts from osteoclastomas.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse X63.Ag8.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P06756
- P05106
- Entrez Gene
- ITGB3
- ITGAV
- GO Terms
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0002576 platelet degranulation
- GO:0050839 cell adhesion molecule binding
- GO:0004872 receptor activity
- GO:0005161 platelet-derived growth factor receptor binding
- GO:0008305 integrin complex
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007411 axon guidance
- GO:0042802 identical protein binding
- GO:0010595 positive regulation of endothelial cell migration
- GO:0010745 negative regulation of macrophage derived foam cell differentiation
- GO:0010888 negative regulation of lipid storage
- GO:0014909 smooth muscle cell migration
- GO:0030168 platelet activation
- GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
- GO:0031092 platelet alpha granule membrane
- GO:0032147 activation of protein kinase activity
- GO:0032369 negative regulation of lipid transport
- GO:0035295 tube development
- GO:0043184 vascular endothelial growth factor receptor 2 binding
- GO:0044419 interspecies interaction between organisms
- GO:0045124 regulation of bone resorption
- GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050748 negative regulation of lipoprotein metabolic process
- GO:0050900 leukocyte migration
- GO:0060055 angiogenesis involved in wound healing
- GO:0071062 alphav-beta3 integrin-vitronectin complex
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- Regulatory
- For research purposes only
Applications of CD51/CD61 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/40 | 1/80 | |
| Immunofluorescence | |||
| Immunohistology - Frozen 1 | |||
| Immunohistology - Paraffin | |||
| Immunoprecipitation |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for CD51/CD61 antibody
-
Horton, M.A. et al. (1985) Monoclonal antibodies to osteoclastomas (giant cell bone tumors): definition of osteoclast-specific cellular antigens.
Cancer Res. 45 (11 Pt 2): 5663-9. -
Davies, J. et al. (1989) The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor.
J Cell Biol. 109 (4 Pt 1): 1817-26. -
Simpson, A. & Horton, M.A. (1989) Expression of the vitronectin receptor during embryonic development; an immunohistological study of the ontogeny of the osteoclast in the rabbit.
Br J Exp Pathol. 70 (3): 257-65. -
Viertlboeck, B.C, and Göbel, T.W. (2007) Chicken thrombocytes express the CD51/CD61 integrin.
Vet Immunol Immunopathol. 119:137-41. -
Danks, L. et al. (2002) Synovial macrophage-osteoclast differentiation in inflammatory arthritis.
Ann Rheum Dis. 61: 916-21. -
Knowles, H.J. et al. (2010) Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4.
FASEB J. 24: 4648-59. -
Lau, Y.S. et al. (2007) Cellular and humoral mechanisms of osteoclast formation in Ewing's sarcoma
Br J Cancer. 96: 1716-22. -
Lau, Y.S. et al. (2006) Malignant melanoma and bone resorption.
Br J Cancer. 94: 1496-503. -
Mabilleau, G. et al. (2009) Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
PLoS One. 4:e4173. -
Whyte, L.S. et al. (2009) The putative cannabinoid receptor GPR55 affects osteoclast function in vitro and bone mass in vivo.
Proc Natl Acad Sci U S A. 106: 16511-6. -
Zhao W et al. (2015) The Gametocytes of Leucocytozoon sabrazesi Infect Chicken Thrombocytes, Not Other Blood Cells.
PLoS One. 10 (7): e0133478. -
Iseri, V.J. & Klasing, K.C. (2013) Dynamics of the systemic components of the chicken (Gallus gallus domesticus) immune system following activation by Escherichia coli; implications for the costs of immunity.
Dev Comp Immunol. 40 (3-4): 248-57. -
Knowles, H.J. (2017) Hypoxia-Induced Fibroblast Growth Factor 11 Stimulates Osteoclast-Mediated Resorption of Bone.
Calcif Tissue Int. 100 (4): 382-91. -
Larrouture, C.Q. et al. (2021) Loss of mutually protective effects between osteoclasts and chondrocytes in damaged joints drives osteoclast-mediated cartilage degradation via matrix metalloproteinases
bioRxiv. 01 Jan [Epub ahead of print].
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