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CD51/CD61 antibody | 23C6

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Anti Human CD51/CD61 Antibody, clone 23C6 thumbnail image 1 Anti Human CD51/CD61 Antibody, clone 23C6 thumbnail image 2
Anti Human CD51/CD61 Antibody, clone 23C6 gallery image 1
Published customer image:
Mouse anti CD51/CD61 antibody, clone 23C6 (MCA757G) used for identifying vitronectin receptor expression by immunocytochemistry.
Image caption:
IL-32-treatment induced a dose dependent differentiation of osteoclast precursors.
(A) TRAcP and VNR positive staining of newly-formed multinucleated cells differentiated from human PBMCs in the presence of 25 ng/ml of M-CSF±100 ng/ml of IL-32. Black bar represents 50 μm. (B) The number of newly-generated TRAcP positive multinucleated cells is dependent on the concentration of IL-32. (C) Size of newly-generated multinucleated cells is not increased at doses higher than 50 ng/ml of IL-32. (D) The number of nuclei per newly-generated multinucleated cells is significantly increased in a dose-dependent manner with the optimal response at 100 ng/ml of IL-32. P values represent the statistical significances between each group using Mann-Whitney test.

From: Insert article citation with space after colon and Hyperlink to PubMed record.Citation: Mabilleau G, Sabokbar A (2009)
Interleukin-32 Promotes Osteoclast Differentiation but Not Osteoclast Activation.
PLoS ONE 4(1): e4173.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD51/CD61 Antibody, clone 23C6 gallery image 2
Published customer image:
Mouse anti Human CD51/CD61 antibody, clone 23C6 (MCA757G) used for the identification of chicken thrombocytes by immunofluorescence.
Image caption:
Images of infected chicken blood cells that are stained with anti-CD51/CD61 monoclonal antibody and observed under a regular fluorescent microscope. (A-D) stained cells with characteristic of thrombocytes having round nuclei and small pale cytoplasm (green arrows) and a weakly stained parasite-infected cell (white arrows). (E-H) A cluster of five thrombocytes stained red by the anti-CD51/CD61 antibody. (I-L) A stained parasite-infected cell and a stained thrombocyte (small red dot). (M-P) Another stained parasite-infected cell (red). Inset images of enlarged gametocytes in L and P show host cell membrane (arrows) and nuclei of host cells (marked ‘N’). Image A-D are from formaldehyde fixed cells; images E-P are from ‘wet’ IFA without fixation. The white scale bar is 10 μM.

From: Zhao W, Liu J, Xu R, Zhang C, Pang Q, Chen X, et al. (2015)
The Gametocytes of
Leucocytozoon sabrazesi
Infect Chicken Thrombocytes, Not Other Blood Cells.
PLoS ONE 10(7): e0133478.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD51/CD61

Product Type
Monoclonal Antibody
Clone
23C6
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA757GA
Datasheet
C* F IP
MSDS
0.1 mg loader

MCA757G
Datasheet
C* F IF IP
MSDS
0.2 mg loader

Mouse anti Human CD51/CD61 antibody, clone 23C6 recognizes the intact complex formed between the CD51 and CD61 molecules (alpha V and beta 3 integrins). This complex binds vitronectin at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Mouse anti Human CD51/CD61 antibody, clone 23C6 reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Pig
Chicken
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Osteoclasts from osteoclastomas.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse X63.Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P06756
P05106
Entrez Gene
ITGB3
ITGAV
GO Terms
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002576 platelet degranulation
GO:0050839 cell adhesion molecule binding
GO:0004872 receptor activity
GO:0005161 platelet-derived growth factor receptor binding
GO:0008305 integrin complex
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0010595 positive regulation of endothelial cell migration
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010888 negative regulation of lipid storage
GO:0014909 smooth muscle cell migration
GO:0030168 platelet activation
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0031092 platelet alpha granule membrane
GO:0032147 activation of protein kinase activity
GO:0032369 negative regulation of lipid transport
GO:0035295 tube development
GO:0043184 vascular endothelial growth factor receptor 2 binding
GO:0044419 interspecies interaction between organisms
GO:0045124 regulation of bone resorption
GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050748 negative regulation of lipoprotein metabolic process
GO:0050900 leukocyte migration
GO:0060055 angiogenesis involved in wound healing
GO:0071062 alphav-beta3 integrin-vitronectin complex
GO:0005515 protein binding
GO:0007596 blood coagulation
Regulatory
For research purposes only

Applications of CD51/CD61 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/40 1/80
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD51/CD61 antibody

  1. Horton, M.A. et al. (1985) Monoclonal antibodies to osteoclastomas (giant cell bone tumors): definition of osteoclast-specific cellular antigens.
    Cancer Res. 45 (11 Pt 2): 5663-9.
  2. Davies, J. et al. (1989) The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor.
    J Cell Biol. 109 (4 Pt 1): 1817-26.
  3. Simpson, A. & Horton, M.A. (1989) Expression of the vitronectin receptor during embryonic development; an immunohistological study of the ontogeny of the osteoclast in the rabbit.
    Br J Exp Pathol. 70 (3): 257-65.
  4. Viertlboeck, B.C, and Göbel, T.W. (2007) Chicken thrombocytes express the CD51/CD61 integrin.
    Vet Immunol Immunopathol. 119:137-41.
  5. Danks, L. et al. (2002) Synovial macrophage-osteoclast differentiation in inflammatory arthritis.
    Ann Rheum Dis. 61: 916-21.
  6. Knowles, H.J. et al. (2010) Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4.
    FASEB J. 24: 4648-59.
  7. Lau, Y.S. et al. (2007) Cellular and humoral mechanisms of osteoclast formation in Ewing's sarcoma
    Br J Cancer. 96: 1716-22.
  8. Lau, Y.S. et al. (2006) Malignant melanoma and bone resorption.
    Br J Cancer. 94: 1496-503.
  9. Mabilleau, G. et al. (2009) Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
    PLoS One. 4:e4173.
  10. Whyte, L.S. et al. (2009) The putative cannabinoid receptor GPR55 affects osteoclast function in vitro and bone mass in vivo.
    Proc Natl Acad Sci U S A. 106: 16511-6.
  11. Zhao W et al. (2015) The Gametocytes of Leucocytozoon sabrazesi Infect Chicken Thrombocytes, Not Other Blood Cells.
    PLoS One. 10 (7): e0133478.
  12. Iseri, V.J. & Klasing, K.C. (2013) Dynamics of the systemic components of the chicken (Gallus gallus domesticus) immune system following activation by Escherichia coli; implications for the costs of immunity.
    Dev Comp Immunol. 40 (3-4): 248-57.
  13. Knowles, H.J. (2017) Hypoxia-Induced Fibroblast Growth Factor 11 Stimulates Osteoclast-Mediated Resorption of Bone.
    Calcif Tissue Int. 100 (4): 382-91.
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