CD51/CD61 antibody | 23C6

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Anti Human CD51/CD61 Antibody, clone 23C6 thumbnail image 1

Anti Human CD51/CD61 Antibody, clone 23C6 thumbnail image 2

Anti Human CD51/CD61 Antibody, clone 23C6 gallery image 1 — **Published customer image:**
Mouse anti CD51/CD61 antibody, clone 23C6 (**MCA757G**) used for identifying vitronectin receptor expression by immunocytochemistry.
**Image caption:**
IL-32-treatment induced a dose dependent differentiation of osteoclast precursors.
(A) TRAcP and VNR positive staining of newly-formed multinucleated cells differentiated from human PBMCs in the presence of 25 ng/ml of M-CSF±100 ng/ml of IL-32. Black bar represents 50 μm. (B) The number of newly-generated TRAcP positive multinucleated cells is dependent on the concentration of IL-32. (C) Size of newly-generated multinucleated cells is not increased at doses higher than 50 ng/ml of IL-32. (D) The number of nuclei per newly-generated multinucleated cells is significantly increased in a dose-dependent manner with the optimal response at 100 ng/ml of IL-32. P values represent the statistical significances between each group using Mann-Whitney test.

From: Insert article citation with space after colon and Hyperlink to PubMed record.Citation: Mabilleau G, Sabokbar A (2009)
Interleukin-32 Promotes Osteoclast Differentiation but Not Osteoclast Activation.
PLoS ONE 4(1): e4173.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Human CD51/CD61 Antibody, clone 23C6 gallery image 2 — **Published customer image:**
Mouse anti Human CD51/CD61 antibody, clone 23C6 (**MCA757G**) used for the identification of chicken thrombocytes by immunofluorescence.
**Image caption:**
Images of infected chicken blood cells that are stained with anti-CD51/CD61 monoclonal antibody and observed under a regular fluorescent microscope. (A-D) stained cells with characteristic of thrombocytes having round nuclei and small pale cytoplasm (green arrows) and a weakly stained parasite-infected cell (white arrows). (E-H) A cluster of five thrombocytes stained red by the anti-CD51/CD61 antibody. (I-L) A stained parasite-infected cell and a stained thrombocyte (small red dot). (M-P) Another stained parasite-infected cell (red). Inset images of enlarged gametocytes in L and P show host cell membrane (arrows) and nuclei of host cells (marked ‘N’). Image A-D are from formaldehyde fixed cells; images E-P are from ‘wet’ IFA without fixation. The white scale bar is 10 μM.

From: Zhao W, Liu J, Xu R, Zhang C, Pang Q, Chen X, *et al*. (2015)
The Gametocytes of
Leucocytozoon sabrazesi
Infect Chicken Thrombocytes, Not Other Blood Cells.
PLoS ONE 10(7): e0133478.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD51/CD61

Product Type: Monoclonal Antibody
Clone: 23C6
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA757GA	C* F IP			0.1 mg
MCA757G	C* F IF IP			0.2 mg

Mouse anti Human CD51/CD61 antibody, clone 23C6 recognizes the intact complex formed between the CD51 and CD61 molecules (alpha V and beta 3 integrins). This complex binds vitronectin at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Mouse anti Human CD51/CD61 antibody, clone 23C6 reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Bovine
Pig
Chicken
Rabbit

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Osteoclasts from osteoclastomas.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised mice were fused with cells of the mouse X63.Ag8.653 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch

More Information

UniProt: P06756 Related reagents; P05106 Related reagents
Entrez Gene: ITGB3 Related reagents; ITGAV Related reagents
GO Terms: GO:0001938 positive regulation of endothelial cell proliferation; GO:0002576 platelet degranulation; GO:0050839 cell adhesion molecule binding; GO:0004872 receptor activity; GO:0005161 platelet-derived growth factor receptor binding; GO:0008305 integrin complex; GO:0007160 cell-matrix adhesion; GO:0007229 integrin-mediated signaling pathway; GO:0007411 axon guidance; GO:0042802 identical protein binding; GO:0010595 positive regulation of endothelial cell migration; GO:0010745 negative regulation of macrophage derived foam cell differentiation; GO:0010888 negative regulation of lipid storage; GO:0014909 smooth muscle cell migration; GO:0030168 platelet activation; GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway; GO:0031092 platelet alpha granule membrane; GO:0032147 activation of protein kinase activity; GO:0032369 negative regulation of lipid transport; GO:0035295 tube development; GO:0043184 vascular endothelial growth factor receptor 2 binding; GO:0044419 interspecies interaction between organisms; GO:0045124 regulation of bone resorption; GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation; GO:0050748 negative regulation of lipoprotein metabolic process; GO:0050900 leukocyte migration; GO:0060055 angiogenesis involved in wound healing; GO:0071062 alphav-beta3 integrin-vitronectin complex; GO:0005515 protein binding; GO:0007596 blood coagulation
Regulatory: For research purposes only

Applications of CD51/CD61 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	1/40	1/80
Immunofluorescence
Immunohistology - Frozen ¹
Immunohistology - Paraffin
Immunoprecipitation

¹The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD51/CD61 antibody

Horton, M.A. et al. (1985) Monoclonal antibodies to osteoclastomas (giant cell bone tumors): definition of osteoclast-specific cellular antigens.
Cancer Res. 45 (11 Pt 2): 5663-9.
Davies, J. et al. (1989) The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor.
J Cell Biol. 109 (4 Pt 1): 1817-26.
Simpson, A. & Horton, M.A. (1989) Expression of the vitronectin receptor during embryonic development; an immunohistological study of the ontogeny of the osteoclast in the rabbit.
Br J Exp Pathol. 70 (3): 257-65.
Viertlboeck, B.C, and Göbel, T.W. (2007) Chicken thrombocytes express the CD51/CD61 integrin.
Vet Immunol Immunopathol. 119:137-41.
Danks, L. et al. (2002) Synovial macrophage-osteoclast differentiation in inflammatory arthritis.
Ann Rheum Dis. 61: 916-21.
Knowles, H.J. et al. (2010) Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4.
FASEB J. 24: 4648-59.
Lau, Y.S. et al. (2007) Cellular and humoral mechanisms of osteoclast formation in Ewing's sarcoma
Br J Cancer. 96: 1716-22.
Lau, Y.S. et al. (2006) Malignant melanoma and bone resorption.
Br J Cancer. 94: 1496-503.
Mabilleau, G. et al. (2009) Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
PLoS One. 4:e4173.
Whyte, L.S. et al. (2009) The putative cannabinoid receptor GPR55 affects osteoclast function in vitro and bone mass in vivo.
Proc Natl Acad Sci U S A. 106: 16511-6.
Zhao W et al. (2015) The Gametocytes of Leucocytozoon sabrazesi Infect Chicken Thrombocytes, Not Other Blood Cells.
PLoS One. 10 (7): e0133478.
Iseri, V.J. & Klasing, K.C. (2013) Dynamics of the systemic components of the chicken (Gallus gallus domesticus) immune system following activation by Escherichia coli; implications for the costs of immunity.
Dev Comp Immunol. 40 (3-4): 248-57.
Knowles, H.J. (2017) Hypoxia-Induced Fibroblast Growth Factor 11 Stimulates Osteoclast-Mediated Resorption of Bone.
Calcif Tissue Int. 100 (4): 382-91.

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