CD49f antibody | 450-30A

Mouse anti Human CD49f

Product Type
Monoclonal Antibody
Clone
450-30A
Isotype
IgG1
Specificity
CD49f

Product Code Applications Pack Size List Price Your Price Qty
MCA1457GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F WB 0.1 mg loader
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loader
MCA1457
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F WB 0.2 mg loader
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loader
MCA1457T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F WB 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD49f antibody, clone 450-30A recognizes the human VLA-6 cell surface antigen, also known as the alpha 6 integrin and as CD49f.

CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes.

CD49f is also widely expressed on epithelial tissues.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
alpha 6 beta 4 integrin purified from A431 cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunohistology - Frozen 1 1/50 1/100
Immunohistology - Paraffin
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Tonsil

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD49f antibody

  1. Kennel, S.J. et al. (1990) Second generation monoclonal antibodies to the human integrin alpha 6 beta 4.
    Hybridoma. 9 (3): 243-55.
  2. Maurice, S. et al. (2007) Isolation of progenitor cells from cord blood using adhesion matrices.
    Cytotechnology. 54: 121-33.
  3. Cavers, M. et al. (2002) Differential expression of beta1 and beta2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations.
    Clin Exp Immunol. 127: 60-5.
  4. López, J. et al. (2012) Cancer-initiating cells derived from established cervical cell lines exhibit stem-cell markers and increased radioresistance.
    BMC Cancer. 12: 48.
  5. Keller, P.J. et al. (2012) Defining the cellular precursors to human breast cancer.
    Proc Natl Acad Sci U S A. 109: 2772-7.
  6. Kaczmarek, M. et al. (2011) Evaluation of the phenotype pattern of macrophages isolated from malignant and non-malignant pleural effusions.
    Tumour Biol. 32: 1123-32.
  7. Aldridge, V. et al. (2012) Human mesenchymal stem cells are recruited to injured liver in a β1-integrin and CD44 dependent manner.
    Hepatology. 56 (3): 1063-73.
  8. Steinberg, F. et al. (2012) SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.
    J Cell Biol. 197 (2): 219-30.
  9. View The Latest Product References
  10. Liu, L. et al. (2003) Priming of eosinophil migration across lung epithelial cell monolayers and upregulation of CD11b/CD18 are elicited by extracellular Ca2+.
    Am J Respir Cell Mol Biol. 28: 713-21.
  11. Goyer, B. et al. (2017) Extracellular matrix and integrin expression profiles in Fuchs endothelial corneal dystrophy cells and tissue model.
    Tissue Eng Part A. Jul 20 [Epub ahead of print].

Flow Cytometry

Synonyms
Integrin Alpha 6 Chain
VLA-6
RRID
AB_321464
UniProt
P23229
Entrez Gene
ITGA6
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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