CD49f antibody | 450-30A
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F WB ResetMouse anti Human CD49f
- Product Type
- Monoclonal Antibody
- Clone
- 450-30A
- Isotype
- IgG1
- Specificity
- CD49f
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| Mouse anti Human CD49f antibody, clone 450-30A recognizes the human VLA-6 cell surface antigen, also known as the alpha 6 integrin and as CD49f. CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes. CD49f is also widely expressed on epithelial tissues. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- alpha 6 beta 4 integrin purified from A431 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunohistology - Frozen 1 | |
1/50 | 1/100 |
| Immunohistology - Paraffin | |||
| Western Blotting | |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD49f antibody
-
Kennel, S.J. et al. (1990) Second generation monoclonal antibodies to the human integrin alpha 6 beta 4.
Hybridoma. 9 (3): 243-55. -
Maurice, S. et al. (2007) Isolation of progenitor cells from cord blood using adhesion matrices.
Cytotechnology. 54: 121-33. -
Cavers, M. et al. (2002) Differential expression of beta1 and beta2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations.
Clin Exp Immunol. 127: 60-5. -
López, J. et al. (2012) Cancer-initiating cells derived from established cervical cell lines exhibit stem-cell markers and increased radioresistance.
BMC Cancer. 12: 48. -
Keller, P.J. et al. (2012) Defining the cellular precursors to human breast cancer.
Proc Natl Acad Sci U S A. 109: 2772-7. -
Kaczmarek, M. et al. (2011) Evaluation of the phenotype pattern of macrophages isolated from malignant and non-malignant pleural effusions.
Tumour Biol. 32: 1123-32. -
Aldridge, V. et al. (2012) Human mesenchymal stem cells are recruited to injured liver in a β1-integrin and CD44 dependent manner.
Hepatology. 56 (3): 1063-73. -
Steinberg, F. et al. (2012) SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.
J Cell Biol. 197 (2): 219-30.
View The Latest Product References
-
Liu, L. et al. (2003) Priming of eosinophil migration across lung epithelial cell monolayers and upregulation of CD11b/CD18 are elicited by extracellular Ca2+.
Am J Respir Cell Mol Biol. 28: 713-21. -
Goyer, B. et al. (2017) Extracellular matrix and integrin expression profiles in Fuchs endothelial corneal dystrophy cells and tissue model.
Tissue Eng Part A. Jul 20 [Epub ahead of print].
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