CD49f antibody | 450-30A

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Mouse anti Human CD49f:FITC

Mouse anti Human CD49f

Mouse anti Human CD49f:RPE

Product Type
Monoclonal Antibody
Clone
450-30A
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1457FT F 25 µg
MCA1457F F 0.1 mg
MCA1457T C* F WB 25 µg
MCA1457GA C* F WB 0.1 mg
MCA1457 C* F WB 0.2 mg
MCA1457PET F 25 Tests
MCA1457PE F 100 Tests
Mouse anti Human CD49f antibody, clone 450-30A recognizes the human VLA-6 cell surface antigen, also known as the alpha 6 integrin and as CD49f.

CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes.

CD49f is also widely expressed on epithelial tissues.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 25 Tests
Reconstitute in 0.25 ml disilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
alpha 6 beta 4 integrin purified from A431 cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P23229 Related reagents
Entrez Gene
ITGA6 Related reagents
Regulatory
For research purposes only

Applications of CD49f antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/10 1/50
Immunohistology - Frozen 1 1/50 1/100
Immunohistology - Paraffin
Western Blotting
Flow Cytometry Neat
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Tonsil

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD49f antibody

  1. Kennel, S.J. et al. (1990) Second generation monoclonal antibodies to the human integrin alpha 6 beta 4.
    Hybridoma. 9 (3): 243-55.
  2. Maurice, S. et al. (2007) Isolation of progenitor cells from cord blood using adhesion matrices.
    Cytotechnology. 54: 121-33.
  3. Cavers, M. et al. (2002) Differential expression of beta1 and beta2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations.
    Clin Exp Immunol. 127: 60-5.
  4. López, J. et al. (2012) Cancer-initiating cells derived from established cervical cell lines exhibit stem-cell markers and increased radioresistance.
    BMC Cancer. 12: 48.
  5. Keller, P.J. et al. (2012) Defining the cellular precursors to human breast cancer.
    Proc Natl Acad Sci U S A. 109: 2772-7.
  6. Kaczmarek, M. et al. (2011) Evaluation of the phenotype pattern of macrophages isolated from malignant and non-malignant pleural effusions.
    Tumour Biol. 32: 1123-32.
  7. Aldridge, V. et al. (2012) Human mesenchymal stem cells are recruited to injured liver in a β1-integrin and CD44 dependent manner.
    Hepatology. 56 (3): 1063-73.
  8. Steinberg, F. et al. (2012) SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.
    J Cell Biol. 197 (2): 219-30.
  9. Liu, L. et al. (2003) Priming of eosinophil migration across lung epithelial cell monolayers and upregulation of CD11b/CD18 are elicited by extracellular Ca2+.
    Am J Respir Cell Mol Biol. 28: 713-21.
  10. Goyer, B. et al. (2017) Extracellular matrix and integrin expression profiles in Fuchs endothelial corneal dystrophy cells and tissue model.
    Tissue Eng Part A. Jul 20 [Epub ahead of print].