CD49d antibody | HP2/1
Filter by Application:F IP WB FN Reset
Mouse anti Human CD49d
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD49d monoclonal antibody, clone HP2/1 recognizes human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form the Peyer's patch-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer’s patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997).
Mouse anti human CD49d, clone HP2/1 binds to both intact and the 80kDa fragment of integrin alpha-4. CD49d is expressed on monocytes, T cells, B cells, thymocytes and Langerhans cells (de Graaf et al. 1995).
Mouse anti Human CD49d, clone HP2/1 can be used in assays of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure and inhibits cell binding to soluble VCAM-1 (Weller et al. 1991).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat Rhesus Monkey Bovine Pig Cynomolgus monkey Goat Rabbit Llama Horse Mink Mustelid Cat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- MCA697: Purified IgG prepared from tissue culture supernatant
- MCA697GA: Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Pack Size: 0.2 mg
0.1% Sodium AzidePack Size: 0.1 mg<0.1% Sodium Azide (NaN3)
- JM leukaemia line.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63 Ag8.653 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry||1ug/5 x 105 cells|
|Functional Assays 1|
|Immunohistology - Frozen|
- 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
References for CD49d antibody
Sánchez-Madrid, F. et al. (1986) VLA-3: a novel polypeptide association within the VLA molecular complex: cell distribution and biochemical characterization.
Eur J Immunol. 16 (11): 1343-9.
Weller, P. F. et al. (1991) Human eosinophil adherence to vascular endothelium mediated by binding to vascular cell adhesion molecule 1 and endothelial leukocyte adhesion molecule 1.
Proc Natl Acad Sci U S A. 88: 7430-3.
Mattila, P. et al. (1992) VLA-4 integrin on sarcoma cell lines recognizes endothelial VCAM-1. Differential regulation of the VLA-4 avidity on various sarcoma cell lines.
Int J Cancer. 52 (6): 918-23.
Kumagai, M. et al. (1995) The cross-reactivity of anti-human adhesion mAb with primate and swine cells.
Leucocyte Typing V. Oxford University Press p. 1646-8.
Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
Cytometry. 29 (4): 351-62.
Van Vliet, S. S. et al. (1995) Species cross reactivity (human-monkey-pig-bovine) of the adhesion structure section mAB.
Leucocyte Typing V. Oxford University Press p 1607-8.
Meister, R.K. et al. (2007) Progress in the discovery and definition of monoclonal antibodies for use in feline research.
Vet Immunol Immunopathol. 119: 38-46.
Bode, U. et al. (2008) Dendritic cell subsets in lymph nodes are characterized by the specific draining area and influence the phenotype and fate of primed T cells.
Immunology. 123: 480-90.
View The Latest Product References
Canalli, A.A. et al. (2011) Participation of Mac-1, LFA-1 and VLA-4 integrins in the in vitro adhesion of sickle cell disease neutrophils to endothelial layers, and reversal of adhesion by simvastatin.
Haematologica. 96: 526-33.
Foster, G.R. et al. (2004) IFN-alpha subtypes differentially affect human T cell motility.
J Immunol. 173: 1663-70.
Haworth, O. et al. (2008) A role for the integrin alpha6beta1 in the differential distribution of CD4 and CD8 T-cell subsets within the rheumatoid synovium.
Rheumatology (Oxford). 47: 1329-34.
Nussbaum, G. et al. (2006) Peptide p277 of HSP60 signals T cells: inhibition of inflammatory chemotaxis.
Int Immunol. 18: 1413-9.
Peled, A. et al. (2002) Immature leukemic CD34+CXCR4+ cells from CML patients have lower integrin-dependent migration and adhesion in response to the chemokine SDF-1.
Stem Cells. 20: 259-66.
Ross, E.A. et al. (2006) Interaction between integrin alpha9beta1 and vascular cell adhesion molecule-1 (VCAM-1) inhibits neutrophil apoptosis.
Blood. 107: 1178-83.
Rosseau, S. et al. (2005) Moraxella catarrhalis--infected alveolar epithelium induced monocyte recruitment and oxidative burst.
Am J Respir Cell Mol Biol. 32: 157-66.
Spring, F.A.et al. (2001) Intercellular adhesion molecule-4 binds alpha(4)beta(1) and alpha(V)-family integrins through novel integrin-binding mechanisms.
Blood. 98: 458-66.
Hyduk, S.J. et al. (2011) Talin-1 and kindlin-3 regulate alpha4beta1 integrin-mediated adhesion stabilization, but not G protein-coupled receptor-induced affinity upregulation.
J Immunol. 187: 4360-8.
Caldwell, J.M. et al. (2017) Cadherin 26 is an alpha integrin-binding epithelial receptor regulated during allergic inflammation.
Mucosal Immunol. Jan 4. [Epub ahead of print]
Uitterdijk, A. et al. (2017) Time course of VCAM-1 expression in reperfused myocardial infarction in swine and its relation to retention of intracoronary administered bone marrow-derived mononuclear cells.
PLoS One. 12 (6): e0178779.
Lokugamage, N. et al. (2021) Use of a small molecule integrin activator as a systemically administered vaccine adjuvant in controlling Chagas disease.
NPJ Vaccines. 6 (1): 114.
Schmitz, J.E. et al. (2001) Simian immunodeficiency virus (SIV)-specific cytotoxic T lymphocytes in gastrointestinal tissues of chronically SIV-infected rhesus monkeys.
Blood. 98 (13): 3757-61.
Sanz, M.J. et al. (1997) Tumor necrosis factor alpha-induced eosinophil accumulation in rat skin is dependent on alpha4 integrin/vascular cell adhesion molecule-1 adhesion pathways.
Blood. 90 (10): 4144-52.
Kuroda, M.J. et al. (1999) Comparative analysis of cytotoxic T lymphocytes in lymph nodes and peripheral blood of simian immunodeficiency virus-infected rhesus monkeys.
J Virol. 73 (2): 1573-9.
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Integrin Alpha 4 Chain
- Entrez Gene
- GO Terms
- GO:0007596 blood coagulation
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0042802 identical protein binding
- GO:0030183 B cell differentiation
- GO:0050776 regulation of immune response
- GO:0050900 leukocyte migration
- View More GO Terms
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.Yes, sign me up