CD49d antibody | 44H6
RPE conjugated Mouse anti Human CD49d antibody, clone 44H6 (MCA923PE) used to evaluate CD49d expression on human brain derived cell lines by flow cytometry.
Image caption:
Brain-derived cell lines express markers for mesenchymal stem cells and pericytes but not for glial or neuronal precursors.(A) Representative histogram of flow cytometry analysis of cortical line and (B) ventricular zone line. Progenitors from all donors and both regions highly express MSC (CD90, CD73, CD105, CD29, CD166 and CD49d) and pericyte markers (CD140b/PDGFR-β, RGS5, CD146, Nestin, α-SMA and NG2). They do not express hematopoietic (CD45), endothelial (CD31, CD34), microglial (CD14, CD11), glial or neuronal precursor cell markers (GFAP, O4) or myofibroblast markers (CD56), (green = isotype, red = respective marker).
From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, Visse E, et al. (2012)
The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.
PLoS One. 2012;7(4):e35577.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
RPE conjugated Mouse anti Human CD49d antibody, clone 44H6 (MCA923PE) used to evaluate CD49d expression on human brain derived cell lines by flow cytometry.
Image caption:
Brain-derived cell lines express markers for mesenchymal stem cells and pericytes but not for glial or neuronal precursors.(A) Representative histogram of flow cytometry analysis of cortical line and (B) ventricular zone line. Progenitors from all donors and both regions highly express MSC (CD90, CD73, CD105, CD29, CD166 and CD49d) and pericyte markers (CD140b/PDGFR-β, RGS5, CD146, Nestin, α-SMA and NG2). They do not express hematopoietic (CD45), endothelial (CD31, CD34), microglial (CD14, CD11), glial or neuronal precursor cell markers (GFAP, O4) or myofibroblast markers (CD56), (green = isotype, red = respective marker).
From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, Visse E, et al. (2012)
The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.
PLoS One. 2012;7(4):e35577.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
RPE conjugated Mouse anti Human CD49d antibody, clone 44H6 (MCA923PE) used to evaluate CD49d expression on human clonal perivascular MSC progenitor lines by flow cytometry.
Image caption:
Characterisation of clonal perivascular MSC progenitor lines.
(B) Clonally derived MSC show the same antigen profile as detected for polyclonal lines with pericyte and MSC markers but neither neuronal nor glial precursor cell markers (O4, GFAP) (example here cortical clone; green = isotype, red = respective marker
From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, Visse E, et al. (2012)
The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.
PLoS One. 2012;7(4):e35577.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD49d
- Product Type
- Monoclonal Antibody
- Clone
- 44H6
- Isotype
- IgG1
- Specificity
- CD49d
Antibody Quality and Performance Guarantee
| Mouse anti Human CD49d monoclonal antibody, clone 44H6 recognizes human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield fragments of 80 and 70 kDa (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer's patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer's patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997) Mouse anti human CD49d, clone 44H6 is reported to induce homotypic aggregation of pre-B cell lines including HOON and NALM-6 (Letarte et al. 1993). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- MCA923: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA923GA: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- HOON pre-B leukaemia cell line.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2.0-Ag14 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/40 | |
| Immunohistology - Frozen 1 | |
1/50 | 1/100 |
| Immunohistology - Paraffin |
- 1Overnight incubation with clone 44H6 is recommended for optimal results. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 100ul of human whole peripheral blood.
- Histology Positive Control Tissue
- Human Tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
References for CD49d antibody
-
Quackenbush, E.J. & Letarte, M. (1985) Identification of several cell surface proteins of non-T, non-B acute lymphoblastic leukemia by using monoclonal antibodies.
J Immunol. 134 (2): 1276-85. -
Letarte, M. et al. (1993) Homotypic aggregation of pre-B leukemic cell lines by antibodies to VLA integrins correlates with their expression of CD9.
Leukemia 7(1): 93-103. -
Hartz B et al. (2011) α 4 integrin levels on mobilized peripheral blood stem cells predict rapidity of engraftment in patients receiving autologous stem cell transplantation.
Blood. 118 (8): 2362-5. -
Ling, K.L. et al. (2007) Modulation of CD103 expression on human colon carcinoma-specific CTL.
J Immunol. 178: 2908-15. -
Eigenmann, P.A. et al. (1999) The mucosal adhesion receptor alpha4beta7 integrin is selectively increased in lymphocytes stimulated with beta-lactoglobulin in children allergic to cow's milk.
J Allergy Clin Immunol. 103: 931-6. -
Rabe, H. et al. (2011) Higher proportions of circulating FOXP3+ and CTLA-4+ regulatory T cells are associated with lower fractions of memory CD4+ T cells in infants.
J Leukoc Biol. 90: 1133-40. -
Seoh, J.Y. et al. (2001) Cell cycling status of human cord blood CD34+ cells during ex vivo expansion is related to the level of very late antigen expression.
J Korean Med Sci. 16: 20-4. -
Seoh, J.Y. (2001) VLA-4 expression and cell cycling status during ex vivo expansion of human cord blood CD34+ cells.
Acta Haematol. 105: 111-5.
View The Latest Product References
-
Dogan, A. et al. (1997) Expression of lymphocyte homing receptors and vascular addressins in low-grade gastric B-cell lymphomas of mucosa-associated lymphoid tissue.
Am J Pathol. 151: 1361-9. -
La Heij, E. et al. (1998) Adhesion molecules in iris biopsy specimens from patients with uveitis.
Br J Ophthalmol. 82: 432-7. -
Humphries, J.D. and Humphries, M.J. (2007) CD14 is a ligand for the integrin alpha4beta1.
FEBS Lett. 581: 757-63. -
Grindebacke, H. et al. (2009) Dynamic development of homing receptor expression and memory cell differentiation of infant CD4+CD25high regulatory T cells.
J Immunol. 183: 4360-70. -
Kim, T.J. ,i>et al. (2010) Suppression of human anti-porcine natural killer cell xenogeneic responses by combinations of monoclonal antibodies specific to CD2 and NKG2D and extracellular signal-regulated kinase kinase inhibitor.
Immunology. 130: 545-55. -
Stadlmann, S. et al. (2003) Disruption of the integrity of human peritoneal mesothelium by interleukin-1beta and tumor necrosis factor-alpha.
Virchows Arch. 443: 678-85. -
Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577. -
Spring, F.A. et al. (2001) Intercellular adhesion molecule-4 binds alpha(4)beta(1) and alpha(V)-family integrins through novel integrin-binding mechanisms.
Blood. 98: 458-66. -
Woolhouse, I.S. et al. (2005) Endothelial interactions of neutrophils under flow in chronic obstructive pulmonary disease.
Eur Respir J. 25: 612-7. -
McGilvray, I.D. et al. (1997) VLA-4 integrin cross-linking on human monocytic THP-1 cells induces tissue factor expression by a mechanism involving mitogen-activated protein kinase.
J Biol Chem. 272: 10287-94. -
Sakamoto, T.M. et al. (2013) Altered red cell and platelet adhesion in hemolytic diseases: Hereditary spherocytosis, paroxysmal nocturnal hemoglobinuria and sickle cell disease.
Clin Biochem. pii: S0009-9120(13)00442-6. -
Zucchetto, A. et al. (2012) The CD49d/CD29 complex is physically and functionally associated with CD38 in B-cell chronic lymphocytic leukemia cells.
Leukemia. 26 (6): 1301-12. -
Strömbeck, A. et al. (2014) High proportions of FOXP3(+) CD25(high) T cells in neonates are positively associated with allergic sensitization later in childhood.
Clin Exp Allergy. 44 (7): 940-52. -
Dintwe, O.B. et al. (2013) Heterologous vaccination against human tuberculosis modulates antigen-specific CD4+ T-cell function.
Eur J Immunol. 43 (9): 2409-20.
- Synonyms
- Integrin Alpha 4 Chain
- VLA-4
- RRID
- AB_321450
- UniProt
- P13612
- Entrez Gene
- ITGA4
- GO Terms
- GO:0007596 blood coagulation
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0042802 identical protein binding
- GO:0030183 B cell differentiation
- GO:0050776 regulation of immune response
- GO:0050900 leukocyte migration
- View More GO Terms
MCA923
1607
1801
MCA923GA
1801
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up