CD49d antibody | 44H6
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|Mouse anti Human CD49d monoclonal antibody, clone 44H6 recognizes human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield fragments of 80 and 70 kDa (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer's patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer's patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997)
Mouse anti human CD49d, clone 44H6 is reported to induce homotypic aggregation of pre-B cell lines including HOON and NALM-6 (Letarte et al. 1993).
- Target Species
- Product Form
- Purified IgG - liquid
- MCA923GA: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- MCA923: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- HOON pre-B leukaemia cell line.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2.0-Ag14 mouse myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1||1/50||1/100|
|Immunohistology - Paraffin|
- 1Overnight incubation with clone 44H6 is recommended for optimal results. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 100ul of human whole peripheral blood.
- Histology Positive Control Tissue
- Human Tonsil
References for CD49d antibody
Quackenbush, E.J. & Letarte, M. (1985) Identification of several cell surface proteins of non-T, non-B acute lymphoblastic leukemia by using monoclonal antibodies.
J Immunol. 134 (2): 1276-85.
Letarte, M. et al. (1993) Homotypic aggregation of pre-B leukemic cell lines by antibodies to VLA integrins correlates with their expression of CD9.
Leukemia 7(1): 93-103.
Hartz B et al. (2011) α 4 integrin levels on mobilized peripheral blood stem cells predict rapidity of engraftment in patients receiving autologous stem cell transplantation.
Blood. 118 (8): 2362-5.
Ling, K.L. et al. (2007) Modulation of CD103 expression on human colon carcinoma-specific CTL.
J Immunol. 178: 2908-15.
Eigenmann, P.A. et al. (1999) The mucosal adhesion receptor alpha4beta7 integrin is selectively increased in lymphocytes stimulated with beta-lactoglobulin in children allergic to cow's milk.
J Allergy Clin Immunol. 103: 931-6.
Rabe, H. et al. (2011) Higher proportions of circulating FOXP3+ and CTLA-4+ regulatory T cells are associated with lower fractions of memory CD4+ T cells in infants.
J Leukoc Biol. 90: 1133-40.
Seoh, J.Y. et al. (2001) Cell cycling status of human cord blood CD34+ cells during ex vivo expansion is related to the level of very late antigen expression.
J Korean Med Sci. 16: 20-4.
Seoh, J.Y. (2001) VLA-4 expression and cell cycling status during ex vivo expansion of human cord blood CD34+ cells.
Acta Haematol. 105: 111-5.
View The Latest Product References
Dogan, A. et al. (1997) Expression of lymphocyte homing receptors and vascular addressins in low-grade gastric B-cell lymphomas of mucosa-associated lymphoid tissue.
Am J Pathol. 151: 1361-9.
La Heij, E. et al. (1998) Adhesion molecules in iris biopsy specimens from patients with uveitis.
Br J Ophthalmol. 82: 432-7.
Humphries, J.D. and Humphries, M.J. (2007) CD14 is a ligand for the integrin alpha4beta1.
FEBS Lett. 581: 757-63.
Grindebacke, H. et al. (2009) Dynamic development of homing receptor expression and memory cell differentiation of infant CD4+CD25high regulatory T cells.
J Immunol. 183: 4360-70.
Kim, T.J. ,i>et al. (2010) Suppression of human anti-porcine natural killer cell xenogeneic responses by combinations of monoclonal antibodies specific to CD2 and NKG2D and extracellular signal-regulated kinase kinase inhibitor.
Immunology. 130: 545-55.
Stadlmann, S. et al. (2003) Disruption of the integrity of human peritoneal mesothelium by interleukin-1beta and tumor necrosis factor-alpha.
Virchows Arch. 443: 678-85.
Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577.
Spring, F.A. et al. (2001) Intercellular adhesion molecule-4 binds alpha(4)beta(1) and alpha(V)-family integrins through novel integrin-binding mechanisms.
Blood. 98: 458-66.
Woolhouse, I.S. et al. (2005) Endothelial interactions of neutrophils under flow in chronic obstructive pulmonary disease.
Eur Respir J. 25: 612-7.
McGilvray, I.D. et al. (1997) VLA-4 integrin cross-linking on human monocytic THP-1 cells induces tissue factor expression by a mechanism involving mitogen-activated protein kinase.
J Biol Chem. 272: 10287-94.
Sakamoto, T.M. et al. (2013) Altered red cell and platelet adhesion in hemolytic diseases: Hereditary spherocytosis, paroxysmal nocturnal hemoglobinuria and sickle cell disease.
Clin Biochem. pii: S0009-9120(13)00442-6.
Zucchetto, A. et al. (2012) The CD49d/CD29 complex is physically and functionally associated with CD38 in B-cell chronic lymphocytic leukemia cells.
Leukemia. 26 (6): 1301-12.
Strömbeck, A. et al. (2014) High proportions of FOXP3(+) CD25(high) T cells in neonates are positively associated with allergic sensitization later in childhood.
Clin Exp Allergy. 44 (7): 940-52.
Dintwe, O.B. et al. (2013) Heterologous vaccination against human tuberculosis modulates antigen-specific CD4+ T-cell function.
Eur J Immunol. 43 (9): 2409-20.
- Integrin Alpha 4 Chain
- Entrez Gene
- GO Terms
- GO:0007596 blood coagulation
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0042802 identical protein binding
- GO:0030183 B cell differentiation
- GO:0050776 regulation of immune response
- GO:0050900 leukocyte migration
- View More GO Terms
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