CD49d antibody | 44H6

Mouse anti Human CD49d

Product Type
Monoclonal Antibody
Clone
44H6
Isotype
IgG1
Specificity
CD49d

Product Code Applications Pack Size List Price Your Price Qty
MCA923GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F 0.1 mg loader
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loader
MCA923
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SDS Safety Datasheet SDS
C * F 0.2 mg loader
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loader
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Mouse anti Human CD49d monoclonal antibody, clone 44H6 recognizes human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield fragments of 80 and 70 kDa (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer's patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer's patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997)

Mouse anti human CD49d, clone 44H6 is reported to induce homotypic aggregation of pre-B cell lines including HOON and NALM-6 (Letarte et al. 1993).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA923GA: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
MCA923: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
HOON pre-B leukaemia cell line.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2.0-Ag14 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/40
Immunohistology - Frozen 1 1/50 1/100
Immunohistology - Paraffin
  1. 1Overnight incubation with clone 44H6 is recommended for optimal results. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 100ul of human whole peripheral blood.
Histology Positive Control Tissue
Human Tonsil

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD49d antibody

  1. Quackenbush, E.J. & Letarte, M. (1985) Identification of several cell surface proteins of non-T, non-B acute lymphoblastic leukemia by using monoclonal antibodies.
    J Immunol. 134 (2): 1276-85.
  2. Letarte, M. et al. (1993) Homotypic aggregation of pre-B leukemic cell lines by antibodies to VLA integrins correlates with their expression of CD9.
    Leukemia 7(1): 93-103.
  3. Hartz B et al. (2011) α 4 integrin levels on mobilized peripheral blood stem cells predict rapidity of engraftment in patients receiving autologous stem cell transplantation.
    Blood. 118 (8): 2362-5.
  4. Ling, K.L. et al. (2007) Modulation of CD103 expression on human colon carcinoma-specific CTL.
    J Immunol. 178: 2908-15.
  5. Eigenmann, P.A. et al. (1999) The mucosal adhesion receptor alpha4beta7 integrin is selectively increased in lymphocytes stimulated with beta-lactoglobulin in children allergic to cow's milk.
    J Allergy Clin Immunol. 103: 931-6.
  6. Rabe, H. et al. (2011) Higher proportions of circulating FOXP3+ and CTLA-4+ regulatory T cells are associated with lower fractions of memory CD4+ T cells in infants.
    J Leukoc Biol. 90: 1133-40.
  7. Seoh, J.Y. et al. (2001) Cell cycling status of human cord blood CD34+ cells during ex vivo expansion is related to the level of very late antigen expression.
    J Korean Med Sci. 16: 20-4.
  8. Seoh, J.Y. (2001) VLA-4 expression and cell cycling status during ex vivo expansion of human cord blood CD34+ cells.
    Acta Haematol. 105: 111-5.
  9. View The Latest Product References
  10. Dogan, A. et al. (1997) Expression of lymphocyte homing receptors and vascular addressins in low-grade gastric B-cell lymphomas of mucosa-associated lymphoid tissue.
    Am J Pathol. 151: 1361-9.
  11. La Heij, E. et al. (1998) Adhesion molecules in iris biopsy specimens from patients with uveitis.
    Br J Ophthalmol. 82: 432-7.
  12. Humphries, J.D. and Humphries, M.J. (2007) CD14 is a ligand for the integrin alpha4beta1.
    FEBS Lett. 581: 757-63.
  13. Grindebacke, H. et al. (2009) Dynamic development of homing receptor expression and memory cell differentiation of infant CD4+CD25high regulatory T cells.
    J Immunol. 183: 4360-70.
  14. Kim, T.J. ,i>et al. (2010) Suppression of human anti-porcine natural killer cell xenogeneic responses by combinations of monoclonal antibodies specific to CD2 and NKG2D and extracellular signal-regulated kinase kinase inhibitor.
    Immunology. 130: 545-55.
  15. Stadlmann, S. et al. (2003) Disruption of the integrity of human peritoneal mesothelium by interleukin-1beta and tumor necrosis factor-alpha.
    Virchows Arch. 443: 678-85.
  16. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
    PLoS One. 7: e35577.
  17. Spring, F.A. et al. (2001) Intercellular adhesion molecule-4 binds alpha(4)beta(1) and alpha(V)-family integrins through novel integrin-binding mechanisms.
    Blood. 98: 458-66.
  18. Woolhouse, I.S. et al. (2005) Endothelial interactions of neutrophils under flow in chronic obstructive pulmonary disease.
    Eur Respir J. 25: 612-7.
  19. McGilvray, I.D. et al. (1997) VLA-4 integrin cross-linking on human monocytic THP-1 cells induces tissue factor expression by a mechanism involving mitogen-activated protein kinase.
    J Biol Chem. 272: 10287-94.
  20. Sakamoto, T.M. et al. (2013) Altered red cell and platelet adhesion in hemolytic diseases: Hereditary spherocytosis, paroxysmal nocturnal hemoglobinuria and sickle cell disease.
    Clin Biochem. pii: S0009-9120(13)00442-6.
  21. Zucchetto, A. et al. (2012) The CD49d/CD29 complex is physically and functionally associated with CD38 in B-cell chronic lymphocytic leukemia cells.
    Leukemia. 26 (6): 1301-12.
  22. Strömbeck, A. et al. (2014) High proportions of FOXP3(+) CD25(high) T cells in neonates are positively associated with allergic sensitization later in childhood.
    Clin Exp Allergy. 44 (7): 940-52.
  23. Dintwe, O.B. et al. (2013) Heterologous vaccination against human tuberculosis modulates antigen-specific CD4+ T-cell function.
    Eur J Immunol. 43 (9): 2409-20.

Flow Cytometry

Functional Assays

Immunofluorescence

Synonyms
Integrin Alpha 4 Chain
VLA-4
RRID
AB_321450
UniProt
P13612
Entrez Gene
ITGA4
GO Terms
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0042802 identical protein binding
GO:0030183 B cell differentiation
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration
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