CD48 antibody | MEM-102
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|Mouse anti Human CD48 antibody, clone MEM-102 recognizes human CD48, also known as B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2 or Signaling lymphocytic activation molecule 2. CD 48, as a mature molecule is a 193 amino acid, ~45 kDa cell membrane glycoprotein containing 2 Ig-like domains.
CD48 acts as a counter-receptor for CD2 (Kato et al. 1992) and is involved in immune regulation and tolerance (McArdel et al. 2016)
Mouse anti Human CD48 antibody, clone MEM-102 is useful for the visualisation of CD48 expressing cells by immunofluorescence (Enose-Akahata et al. 2009) and flow cytometry and may be used to differentiate haemapoietic tumors, sarcomas and melanomas.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rhesus Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- MCA1053G: Purified IgG prepared by affinity chromatography on Protein A
- MCA1053GT: Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/50||1/100|
|Immunohistology - Paraffin|
|Western Blotting 1|
- 1This product recognizes CD48 under non-reducing conditions.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
References for CD48 antibody
Horejsí, V. et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
Folia Biol (Praha). 34 (1): 23-34.
Korínek, V. et al. (1991) The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1.
Immunogenetics. 33 (2): 108-12.
Cinek, T. & Horejsí, V. (1992) The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases.
J Immunol. 149 (7): 2262-70.
Staffler, G. et al. (2003) Selective inhibition of T cell activation via CD147 through novel modulation of lipid rafts.
J Immunol. 171 (4): 1707-14.
Larochelle, A. et al. (2011) Human and rhesus macaque hematopoietic stem cells cannot be purified based only on SLAM family markers.
Enose-Akahata, Y. et al. (2009) High expression of CD244 and SAP regulated CD8 T cell responses of patients with HTLV-I associated neurologic disease.
PLoS Pathog. 5: e1000682.
Fukushima, K. et al. (2005) Functional role played by the glycosylphosphatidylinositol anchor glycan of CD48 in interleukin-18-induced interferon-gamma production.
J Biol Chem. 280: 18056-62.
Kanuga, N. et al. (2002) Characterization of genetically modified human retinal pigment epithelial cells developed for in vitro and transplantation studies.
Invest Ophthalmol Vis Sci. 43: 546-55.
View The Latest Product References
Peterson, L.A. et al. (2019) Individual differences in the response of human β-lymphoblastoid cells to the cytotoxic, mutagenic and DNA damaging effects of a DNA methylating agent, N-methylnitrosourethane.
Chem Res Toxicol. Oct 07 [Epub ahead of print].
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