CD48 antibody | MEM-102

Mouse anti Human CD48

Product Type
Monoclonal Antibody
Clone
MEM-102
Isotype
IgG1
Specificity
CD48

Product Code Applications Pack Size List Price Your Price Qty
MCA1053G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF P WB * 0.2 mg loader
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loader
MCA1053GT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF P WB * 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD48 antibody, clone MEM-102 recognizes human CD48, also known as B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2 or Signaling lymphocytic activation molecule 2. CD 48, as a mature molecule is a 193 amino acid, ~45 kDa cell membrane glycoprotein containing 2 Ig-like domains.

CD48 acts as a counter-receptor for CD2 (Kato et al. 1992) and is involved in immune regulation and tolerance (McArdel et al. 2016)

Mouse anti Human CD48 antibody, clone MEM-102 is useful for the visualisation of CD48 expressing cells by immunofluorescence (Enose-Akahata et al. 2009) and flow cytometry and may be used to differentiate haemapoietic tumors, sarcomas and melanomas.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA1053G: Purified IgG prepared by affinity chromatography on Protein A
MCA1053GT: Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen 1/50 1/100
Immunohistology - Paraffin
Western Blotting 1
  1. 1This product recognizes CD48 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Tonsil

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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loader
Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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loader
Description Mouse IgG1 Negative Control

References for CD48 antibody

  1. Horejsí, V. et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
    Folia Biol (Praha). 34 (1): 23-34.
  2. Korínek, V. et al. (1991) The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1.
    Immunogenetics. 33 (2): 108-12.
  3. Cinek, T. & Horejsí, V. (1992) The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases.
    J Immunol. 149 (7): 2262-70.
  4. Staffler, G. et al. (2003) Selective inhibition of T cell activation via CD147 through novel modulation of lipid rafts.
    J Immunol. 171 (4): 1707-14.
  5. Larochelle, A. et al. (2011) Human and rhesus macaque hematopoietic stem cells cannot be purified based only on SLAM family markers.
    Blood. 117:1550-4.
  6. Enose-Akahata, Y. et al. (2009) High expression of CD244 and SAP regulated CD8 T cell responses of patients with HTLV-I associated neurologic disease.
    PLoS Pathog. 5: e1000682.
  7. Fukushima, K. et al. (2005) Functional role played by the glycosylphosphatidylinositol anchor glycan of CD48 in interleukin-18-induced interferon-gamma production.
    J Biol Chem. 280: 18056-62.
  8. Kanuga, N. et al. (2002) Characterization of genetically modified human retinal pigment epithelial cells developed for in vitro and transplantation studies.
    Invest Ophthalmol Vis Sci. 43: 546-55.
  9. View The Latest Product References
  10. Peterson, L.A. et al. (2019) Individual differences in the response of human β-lymphoblastoid cells to the cytotoxic, mutagenic and DNA damaging effects of a DNA methylating agent, N-methylnitrosourethane.
    Chem Res Toxicol. Oct 07 [Epub ahead of print].

Western Blotting

Synonyms
BLAST-1
RRID
AB_323736
UniProt
P09326
Entrez Gene
CD48
GO Terms
GO:0005515 protein binding
GO:0006952 defense response
GO:0007596 blood coagulation
GO:0005887 integral to plasma membrane
GO:0045121 membrane raft
GO:0050900 leukocyte migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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