CD46 antibody | MEM-258

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Anti Human CD46 Antibody, clone MEM-258 thumbnail image 1

Anti Human CD46 Antibody, clone MEM-258 thumbnail image 2

Anti Human CD46 Antibody, clone MEM-258 thumbnail image 3

Anti Human CD46 Antibody, clone MEM-258 gallery image 1 — Figure A. APC conjugated Mouse anti Human CD3 (MCA463APC) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. APC conjugated Mouse anti Human CD3 (MCA463APC) and RPE conjugated Mouse anti Human CD46 (MCA2113PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Anti Human CD46 Antibody, clone MEM-258 gallery image 2 — **Published customer image:**
Mouse anti Human CD46 antibody, clone MEM-258 (**MCA2113**) used for blocking adenovirus attachment to cells *in vitro* in blocking experiments on smoooth muscle and endothelial cells.
**Image caption:**
*In vitro* virus transduction. β-Gal activity and representative images of X-gal stained cells in A. HSVSMC and B. HSVEC infected with 5000 vp/cell for 3 hours at 37°C. β-Gal activity was normalised to total protein levels (* = p <0.05 compared to Ad5, *** = p <0.001 compared to Ad5). β-Gal activity normalised to total protein in (C) HSVSMC and (D) HSVEC infected with 5000 vp/cell in the presence or absence of CD46 function-blocking antibody MEM258 or an isotype matched control. (* = p <0.05 relative to isotype control, ** = p <0.01 relative to isotype control).

From: White KM, Alba R, Parker AL, Wright AF, Bradshaw AC, Delles C, McDonald RA,Baker AH.
Assessment of a novel, capsid-modified adenovirus with an improved vascular gene transfer profile.
J Cardiothorac Surg. 2013 Aug 9;8:183.

Anti Human CD46 Antibody, clone MEM-258 gallery image 3 — **Published customer image:**
Muse ati HumanCD46 antibody, clone MEM-258 (**MCA2113**) used for the evaluation of CD46 expression on human cell lines by flow cytometry.
**Image caption:**
Detection of CD46, HSPG, CD80, and CD86 surface expression on HeLa, A549, K562, and Ramos cells. Overlays of representative flow cytometry charts are shown.

From: Tuve S, Wang H, Jacobs JD, Yumul RC, Smith DF, Lieber A (2008)
Role of Cellular Heparan Sulfate Proteoglycans in Infection of Human Adenovirus Serotype 3 and 35.
PLoS Pathog 4(10): e1000189.

Mouse anti Human CD46:Alexa Fluor® 647

Mouse anti Human CD46:FITC

Mouse anti Human CD46

Mouse anti Human CD46:RPE

Product Type: Monoclonal Antibody
Clone: MEM-258
Isotype: IgG1

Product Code	Applications	Pack Size
MCA2113A647T	F	25 Tests/0.25ml
MCA2113A647	F	100 Tests/1ml
MCA2113FT	F	25 µg
MCA2113F	F	0.1 mg
MCA2113T	F IF IP WB	25 µg
MCA2113	F FN* IF IP WB	0.2 mg
MCA2113PET	F	25 Tests/0.25ml
MCA2113PE	F	100 Tests

Mouse anti Human CD46 antibody, clone MEM-258 recognizes the human CD46 cell surface antigen, a broadly expressed glycoprotein also known as membrane co-factor protein (MCP).

CD46 functions as an inhibitor of complement activation limiting the formation and activity of C3 convertases. CD46 is expressed by all nucleated cells, often as multiple isoforms on the same cells. The molecule is also expressed by sperm and may be important in the process of fertilisation.

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Pack Size: 25 Tests/0.25ml

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised

Pack Size: 100 Tests

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Pack Size: 25 Tests/0.25ml

Reconstitute with 0.25 ml distilled water

Pack Size: 100 Tests

Reconstitute with 1 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

Pack Size: 25 Tests/0.25ml

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Pack Size: 100 Tests

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Immunogen

HPB-ALL cell line.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Pack Size: 25 Tests/0.25ml
Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Pack Size: 100 Tests
Store at +4^oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P15529 Related reagents
Entrez Gene: CD46 Related reagents
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD46 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry		Neat
Flow Cytometry	1/10	1/50
Functional Assays ¹
Immunofluorescence
Immunoprecipitation
Western Blotting		Non reducing conditions
Flow Cytometry		Neat

¹This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ or 100ul whole blood.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ or 100ul whole blood.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ or 100ul whole blood.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ or 100ul whole blood.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	100 Tests/1ml	F
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Functional Assays

Immunofluorescence

Product Specific References

References for CD46 antibody

Sirena D et al. (2004) The human membrane cofactor CD46 is a receptor for species B adenovirus serotype 3.
J Virol. 78 (9): 4454-62.
Fremeaux-Bacchi, V. et al. (2006) Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome.
J Am Soc Nephrol. 17 (7): 2017-25.
Fleischli, C. et al. (2005) The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35.
J Virol. 79:10013-22.
Sweigard, J.H. et al. (2010) Adenovirus vectors targeting distinct cell types in the retina.
Invest Ophthalmol Vis Sci. 51:2219-28.
Yang, P. et al. (2009) Expression and modulation of RPE cell membrane complement regulatory proteins.
Invest Ophthalmol Vis Sci. 50: 3473-81.
Bahat, A. and Eisenbach, M. (2010) Human sperm thermotaxis is mediated by phospholipase C and inositol trisphosphate receptor Ca2+ channel.
Biol Reprod. 82: 606-16.
Bienaime, F. et al. (2010) Mutations in components of complement influence the outcome of Factor I-associated atypical hemolytic uremic syndrome.
Kidney Int. 77: 339-49.
Wang, H. et al. (2008) In vitro and in vivo properties of adenovirus vectors with increased affinity to CD46.
J Virol. 82: 10567-79.
Hara, H. et al. (2011) Initial in vitro investigation of the human immune response to corneal cells from genetically engineered pigs.
Invest Ophthalmol Vis Sci. 52: 5278-86.
El Karoui, K. et al. (2012) A clinicopathologic study of thrombotic microangiopathy in IgA nephropathy.
J Am Soc Nephrol. 23 (1): 137-48.
Bach, P. et al. (2013) Specific elimination of CD133+ tumor cells with targeted oncolytic measles virus.
Cancer Res. 73 (2): 865-74.
Leaderer, D. et al. (2015) Adeno-associated virus mediated delivery of an engineered protein that combines the complement inhibitory properties of CD46, CD55 and CD59.
J Gene Med. 17 (6-7): 101-15.
Tuve, S. et al. (2006) A new group B adenovirus receptor is expressed at high levels on human stem and tumor cells.
J Virol. 80 (24): 12109-20.
Hara, H. et al. (2008) In vitro investigation of pig cells for resistance to human antibody-mediated rejection.
Transpl Int. 21 (12): 1163-74.
Loré, K. et al. (2007) Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses.
J Immunol. 179 (3): 1721-9.
Fremeaux-Bacchi, V. et al. (2007) Unusual clinical severity of complement membrane cofactor protein-associated hemolytic-uremic syndrome and uniparental isodisomy.
Am J Kidney Dis. 49 (2): 323-9.
Le Quintrec, M. et al. (2008) Complement mutation-associated de novo thrombotic microangiopathy following kidney transplantation.
Am J Transplant. 8 (8): 1694-701.
Boyer, O. et al. (2008) Complement factor H deficiency and posttransplantation glomerulonephritis with isolated C3 deposits.
Am J Kidney Dis. 51 (4): 671-7.
Wang, H. et al. (2009) Receptor usage of a newly emergent adenovirus type 14.
Virology. 387 (2): 436-41.
Iguchi, K. et al. (2012) Efficient antitumor effects of carrier cells loaded with a fiber-substituted conditionally replicating adenovirus on CAR-negative tumor cells.
Cancer Gene Ther. 19 (2): 118-25.
Kälin, S. et al. (2010) Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35.
J Virol. 84 (10): 5336-50.
Bottino, R. et al. (2014) Pig-to-monkey islet xenotransplantation using multi-transgenic pigs.
Am J Transplant. 14 (10): 2275-87.
White, K.M. et al. (2013) Assessment of a novel, capsid-modified adenovirus with an improved vascular gene transfer profile.
J Cardiothorac Surg. 8: 183.
Tuve, S. et al. (2008) Role of cellular heparan sulfate proteoglycans in infection of human adenovirus serotype 3 and 35.
PLoS Pathog. 4 (10): e1000189.
Iwase, H. et al. (2014) Regulation of human platelet aggregation by genetically modified pig endothelial cells and thrombin inhibition.
Xenotransplantation. 21 (1): 72-83.
Sweigard, J.H. et al. (2011) Adenovirus-mediated delivery of CD46 attenuates the alternative complement pathway on RPE: implications for age-related macular degeneration.
Gene Ther. 18 (6): 613-21.

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