CD46 antibody | MEM-258

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Mouse anti Human CD46:Alexa Fluor® 647

Mouse anti Human CD46:FITC

Mouse anti Human CD46

Mouse anti Human CD46:RPE

Product Type
Monoclonal Antibody
Clone
MEM-258
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2113A647T F 25 Tests/0.25ml
MCA2113A647 F 100 Tests/1ml
MCA2113FT F 25 µg
MCA2113F F 0.1 mg
MCA2113T F IF IP WB 25 µg
MCA2113 F FN* IF IP WB 0.2 mg
MCA2113PET F 25 Tests/0.25ml
MCA2113PE F 100 Tests
Mouse anti Human CD46 antibody, clone MEM-258 recognizes the human CD46 cell surface antigen, a broadly expressed glycoprotein also known as membrane co-factor protein (MCP).

CD46 functions as an inhibitor of complement activation limiting the formation and activity of C3 convertases. CD46 is expressed by all nucleated cells, often as multiple isoforms on the same cells. The molecule is also expressed by sperm and may be important in the process of fertilisation.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Pack Size: 25 Tests/0.25ml
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Pack Size: 100 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 25 Tests/0.25ml
Reconstitute with 0.25 ml distilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
Pack Size: 25 Tests/0.25ml
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Pack Size: 100 Tests
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
HPB-ALL cell line.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 25 Tests/0.25ml
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P15529 Related reagents
Entrez Gene
CD46 Related reagents
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD46 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Flow Cytometry 1/10 1/50
Functional Assays 1
Immunofluorescence
Immunoprecipitation
Western Blotting Non reducing conditions
Flow Cytometry Neat
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 or 100ul whole blood.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 100 Tests/1ml F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Functional Assays

Immunofluorescence

Product Specific References

References for CD46 antibody

  1. Sirena D et al. (2004) The human membrane cofactor CD46 is a receptor for species B adenovirus serotype 3.
    J Virol. 78 (9): 4454-62.
  2. Fremeaux-Bacchi, V. et al. (2006) Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome.
    J Am Soc Nephrol. 17 (7): 2017-25.
  3. Fleischli, C. et al. (2005) The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35.
    J Virol. 79:10013-22.
  4. Sweigard, J.H. et al. (2010) Adenovirus vectors targeting distinct cell types in the retina.
    Invest Ophthalmol Vis Sci. 51:2219-28.
  5. Yang, P. et al. (2009) Expression and modulation of RPE cell membrane complement regulatory proteins.
    Invest Ophthalmol Vis Sci. 50: 3473-81.
  6. Bahat, A. and Eisenbach, M. (2010) Human sperm thermotaxis is mediated by phospholipase C and inositol trisphosphate receptor Ca2+ channel.
    Biol Reprod. 82: 606-16.
  7. Bienaime, F. et al. (2010) Mutations in components of complement influence the outcome of Factor I-associated atypical hemolytic uremic syndrome.
    Kidney Int. 77: 339-49.
  8. Wang, H. et al. (2008) In vitro and in vivo properties of adenovirus vectors with increased affinity to CD46.
    J Virol. 82: 10567-79.
  9. Hara, H. et al. (2011) Initial in vitro investigation of the human immune response to corneal cells from genetically engineered pigs.
    Invest Ophthalmol Vis Sci. 52: 5278-86.
  10. El Karoui, K. et al. (2012) A clinicopathologic study of thrombotic microangiopathy in IgA nephropathy.
    J Am Soc Nephrol. 23 (1): 137-48.
  11. Bach, P. et al. (2013) Specific elimination of CD133+ tumor cells with targeted oncolytic measles virus.
    Cancer Res. 73 (2): 865-74.
  12. Leaderer, D. et al. (2015) Adeno-associated virus mediated delivery of an engineered protein that combines the complement inhibitory properties of CD46, CD55 and CD59.
    J Gene Med. 17 (6-7): 101-15.
  13. Tuve, S. et al. (2006) A new group B adenovirus receptor is expressed at high levels on human stem and tumor cells.
    J Virol. 80 (24): 12109-20.
  14. Hara, H. et al. (2008) In vitro investigation of pig cells for resistance to human antibody-mediated rejection.
    Transpl Int. 21 (12): 1163-74.
  15. Loré, K. et al. (2007) Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses.
    J Immunol. 179 (3): 1721-9.
  16. Fremeaux-Bacchi, V. et al. (2007) Unusual clinical severity of complement membrane cofactor protein-associated hemolytic-uremic syndrome and uniparental isodisomy.
    Am J Kidney Dis. 49 (2): 323-9.
  17. Le Quintrec, M. et al. (2008) Complement mutation-associated de novo thrombotic microangiopathy following kidney transplantation.
    Am J Transplant. 8 (8): 1694-701.
  18. Boyer, O. et al. (2008) Complement factor H deficiency and posttransplantation glomerulonephritis with isolated C3 deposits.
    Am J Kidney Dis. 51 (4): 671-7.
  19. Wang, H. et al. (2009) Receptor usage of a newly emergent adenovirus type 14.
    Virology. 387 (2): 436-41.
  20. Iguchi, K. et al. (2012) Efficient antitumor effects of carrier cells loaded with a fiber-substituted conditionally replicating adenovirus on CAR-negative tumor cells.
    Cancer Gene Ther. 19 (2): 118-25.
  21. Kälin, S. et al. (2010) Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35.
    J Virol. 84 (10): 5336-50.
  22. Bottino, R. et al. (2014) Pig-to-monkey islet xenotransplantation using multi-transgenic pigs.
    Am J Transplant. 14 (10): 2275-87.
  23. White, K.M. et al. (2013) Assessment of a novel, capsid-modified adenovirus with an improved vascular gene transfer profile.
    J Cardiothorac Surg. 8: 183.
  24. Tuve, S. et al. (2008) Role of cellular heparan sulfate proteoglycans in infection of human adenovirus serotype 3 and 35.
    PLoS Pathog. 4 (10): e1000189.
  25. Iwase, H. et al. (2014) Regulation of human platelet aggregation by genetically modified pig endothelial cells and thrombin inhibition.
    Xenotransplantation. 21 (1): 72-83.
  26. Sweigard, J.H. et al. (2011) Adenovirus-mediated delivery of CD46 attenuates the alternative complement pathway on RPE: implications for age-related macular degeneration.
    Gene Ther. 18 (6): 613-21.