CD46 antibody | MEM-258

Mouse anti Human CD46

Product Type
Monoclonal Antibody
Clone
MEM-258
Isotype
IgG1
Specificity
CD46

Product Code Applications Pack Size List Price Your Price Qty
MCA2113T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF IP WB 25 µg loader
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loader
MCA2113
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F FN * IF IP WB 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD46 antibody, clone MEM-258 recognizes the human CD46 cell surface antigen, also known as membrane co-factor protein (MCP), Trophoblast leukocyte common antigen or TLX. CD46 is a 392 amino acid (including a 34 aa signal peptide) ~43-60 kDa single pass type 1 transmembrane glysoprotein expressed by all cell types with the exception of erythrocytes.

CD46 functions as areceptor for complement and inhibitor of complement activation, limiting the formation and activity of C3 convertases. CD46 is expressed by all nucleated cells, often as multiple isoforms (Seya et al. 1993) on the same cells. The molecule is also expressed by sperm and may be important in the process of fertilisation (Carver-Ward et al. 1996).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA2113T: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA2113: Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
HPB-ALL cell line.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Functional Assays 1
Immunofluorescence
Immunoprecipitation
Western Blotting Non reducing conditions
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 or 100ul whole blood.

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References for CD46 antibody

  1. Sirena D et al. (2004) The human membrane cofactor CD46 is a receptor for species B adenovirus serotype 3.
    J Virol. 78 (9): 4454-62.
  2. Fremeaux-Bacchi, V. et al. (2006) Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome.
    J Am Soc Nephrol. 17 (7): 2017-25.
  3. Fleischli, C. et al. (2005) The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35.
    J Virol. 79:10013-22.
  4. Sweigard, J.H. et al. (2010) Adenovirus vectors targeting distinct cell types in the retina.
    Invest Ophthalmol Vis Sci. 51:2219-28.
  5. Yang, P. et al. (2009) Expression and modulation of RPE cell membrane complement regulatory proteins.
    Invest Ophthalmol Vis Sci. 50: 3473-81.
  6. Bahat, A. and Eisenbach, M. (2010) Human sperm thermotaxis is mediated by phospholipase C and inositol trisphosphate receptor Ca2+ channel.
    Biol Reprod. 82: 606-16.
  7. Bienaime, F. et al. (2010) Mutations in components of complement influence the outcome of Factor I-associated atypical hemolytic uremic syndrome.
    Kidney Int. 77: 339-49.
  8. Wang, H. et al. (2008) In vitro and in vivo properties of adenovirus vectors with increased affinity to CD46.
    J Virol. 82: 10567-79.
  9. View The Latest Product References
  10. Hara, H. et al. (2011) Initial in vitro investigation of the human immune response to corneal cells from genetically engineered pigs.
    Invest Ophthalmol Vis Sci. 52: 5278-86.
  11. El Karoui, K. et al. (2012) A clinicopathologic study of thrombotic microangiopathy in IgA nephropathy.
    J Am Soc Nephrol. 23 (1): 137-48.
  12. Bach, P. et al. (2013) Specific elimination of CD133+ tumor cells with targeted oncolytic measles virus.
    Cancer Res. 73 (2): 865-74.
  13. Leaderer, D. et al. (2015) Adeno-associated virus mediated delivery of an engineered protein that combines the complement inhibitory properties of CD46, CD55 and CD59.
    J Gene Med. 17 (6-7): 101-15.
  14. Tuve, S. et al. (2006) A new group B adenovirus receptor is expressed at high levels on human stem and tumor cells.
    J Virol. 80 (24): 12109-20.
  15. Hara, H. et al. (2008) In vitro investigation of pig cells for resistance to human antibody-mediated rejection.
    Transpl Int. 21 (12): 1163-74.
  16. Loré, K. et al. (2007) Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses.
    J Immunol. 179 (3): 1721-9.
  17. Fremeaux-Bacchi, V. et al. (2007) Unusual clinical severity of complement membrane cofactor protein-associated hemolytic-uremic syndrome and uniparental isodisomy.
    Am J Kidney Dis. 49 (2): 323-9.
  18. Le Quintrec, M. et al. (2008) Complement mutation-associated de novo thrombotic microangiopathy following kidney transplantation.
    Am J Transplant. 8 (8): 1694-701.
  19. Boyer, O. et al. (2008) Complement factor H deficiency and posttransplantation glomerulonephritis with isolated C3 deposits.
    Am J Kidney Dis. 51 (4): 671-7.
  20. Wang, H. et al. (2009) Receptor usage of a newly emergent adenovirus type 14.
    Virology. 387 (2): 436-41.
  21. Iguchi, K. et al. (2012) Efficient antitumor effects of carrier cells loaded with a fiber-substituted conditionally replicating adenovirus on CAR-negative tumor cells.
    Cancer Gene Ther. 19 (2): 118-25.
  22. Kälin, S. et al. (2010) Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35.
    J Virol. 84 (10): 5336-50.
  23. Bottino, R. et al. (2014) Pig-to-monkey islet xenotransplantation using multi-transgenic pigs.
    Am J Transplant. 14 (10): 2275-87.
  24. White, K.M. et al. (2013) Assessment of a novel, capsid-modified adenovirus with an improved vascular gene transfer profile.
    J Cardiothorac Surg. 8: 183.
  25. Tuve, S. et al. (2008) Role of cellular heparan sulfate proteoglycans in infection of human adenovirus serotype 3 and 35.
    PLoS Pathog. 4 (10): e1000189.
  26. Iwase, H. et al. (2014) Regulation of human platelet aggregation by genetically modified pig endothelial cells and thrombin inhibition.
    Xenotransplantation. 21 (1): 72-83.
  27. Sweigard, J.H. et al. (2011) Adenovirus-mediated delivery of CD46 attenuates the alternative complement pathway on RPE: implications for age-related macular degeneration.
    Gene Ther. 18 (6): 613-21.

Flow Cytometry

Functional Assays

Immunofluorescence

Synonyms
Membrane CO-FACTOR Protein
RRID
AB_323983
UniProt
P15529
Entrez Gene
CD46
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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