CD46 antibody | MEM-258




Mouse anti Human CD46:Alexa Fluor® 647
Mouse anti Human CD46:FITC
Mouse anti Human CD46
Mouse anti Human CD46:RPE
- Product Type
- Monoclonal Antibody
- Clone
- MEM-258
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA2113A647 | F | 100 Tests/1ml |
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MCA2113FT | F | 25 µg |
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MCA2113F | F | 0.1 mg |
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MCA2113T | F IF IP WB | 25 µg |
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MCA2113 | F FN* IF IP WB | 0.2 mg |
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MCA2113PET | F | 25 Tests/0.25ml |
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MCA2113PE | F | 100 Tests |
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CD46 functions as areceptor for complement and inhibitor of complement activation, limiting the formation and activity of C3 convertases. CD46 is expressed by all nucleated cells, often as multiple isoforms (
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilisedPack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Pack Size: 25 Tests/0.25mlReconstitute with 0.25 ml distilled waterPack Size: 100 TestsReconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- Pack Size: 25 Tests/0.25ml
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose Pack Size: 100 Tests0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- HPB-ALL cell line.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 Tests/0.25mlPrior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 100 TestsStore at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P15529
- Entrez Gene
- CD46
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD46 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | ||
Flow Cytometry | 1/10 | 1/50 | |
Functional Assays 1 | |||
Immunofluorescence | |||
Immunoprecipitation | |||
Western Blotting | Non reducing conditions | ||
Flow Cytometry | Neat |
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 or 100ul whole blood.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD46 antibody
-
Sirena D et al. (2004) The human membrane cofactor CD46 is a receptor for species B adenovirus serotype 3.
J Virol. 78 (9): 4454-62. -
Fremeaux-Bacchi, V. et al. (2006) Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome.
J Am Soc Nephrol. 17 (7): 2017-25. -
Fleischli, C. et al. (2005) The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35.
J Virol. 79:10013-22. -
Sweigard, J.H. et al. (2010) Adenovirus vectors targeting distinct cell types in the retina.
Invest Ophthalmol Vis Sci. 51:2219-28. -
Yang, P. et al. (2009) Expression and modulation of RPE cell membrane complement regulatory proteins.
Invest Ophthalmol Vis Sci. 50: 3473-81. -
Bahat, A. and Eisenbach, M. (2010) Human sperm thermotaxis is mediated by phospholipase C and inositol trisphosphate receptor Ca2+ channel.
Biol Reprod. 82: 606-16. -
Bienaime, F. et al. (2010) Mutations in components of complement influence the outcome of Factor I-associated atypical hemolytic uremic syndrome.
Kidney Int. 77: 339-49. -
Wang, H. et al. (2008) In vitro and in vivo properties of adenovirus vectors with increased affinity to CD46.
J Virol. 82: 10567-79. -
Hara, H. et al. (2011) Initial in vitro investigation of the human immune response to corneal cells from genetically engineered pigs.
Invest Ophthalmol Vis Sci. 52: 5278-86. -
El Karoui, K. et al. (2012) A clinicopathologic study of thrombotic microangiopathy in IgA nephropathy.
J Am Soc Nephrol. 23 (1): 137-48. -
Bach, P. et al. (2013) Specific elimination of CD133+ tumor cells with targeted oncolytic measles virus.
Cancer Res. 73 (2): 865-74. -
Leaderer, D. et al. (2015) Adeno-associated virus mediated delivery of an engineered protein that combines the complement inhibitory properties of CD46, CD55 and CD59.
J Gene Med. 17 (6-7): 101-15. -
Tuve, S. et al. (2006) A new group B adenovirus receptor is expressed at high levels on human stem and tumor cells.
J Virol. 80 (24): 12109-20. -
Hara, H. et al. (2008) In vitro investigation of pig cells for resistance to human antibody-mediated rejection.
Transpl Int. 21 (12): 1163-74. -
Loré, K. et al. (2007) Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses.
J Immunol. 179 (3): 1721-9. -
Fremeaux-Bacchi, V. et al. (2007) Unusual clinical severity of complement membrane cofactor protein-associated hemolytic-uremic syndrome and uniparental isodisomy.
Am J Kidney Dis. 49 (2): 323-9. -
Le Quintrec, M. et al. (2008) Complement mutation-associated de novo thrombotic microangiopathy following kidney transplantation.
Am J Transplant. 8 (8): 1694-701. -
Boyer, O. et al. (2008) Complement factor H deficiency and posttransplantation glomerulonephritis with isolated C3 deposits.
Am J Kidney Dis. 51 (4): 671-7. -
Wang, H. et al. (2009) Receptor usage of a newly emergent adenovirus type 14.
Virology. 387 (2): 436-41. -
Iguchi, K. et al. (2012) Efficient antitumor effects of carrier cells loaded with a fiber-substituted conditionally replicating adenovirus on CAR-negative tumor cells.
Cancer Gene Ther. 19 (2): 118-25. -
Kälin, S. et al. (2010) Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35.
J Virol. 84 (10): 5336-50. -
Bottino, R. et al. (2014) Pig-to-monkey islet xenotransplantation using multi-transgenic pigs.
Am J Transplant. 14 (10): 2275-87. -
White, K.M. et al. (2013) Assessment of a novel, capsid-modified adenovirus with an improved vascular gene transfer profile.
J Cardiothorac Surg. 8: 183. -
Tuve, S. et al. (2008) Role of cellular heparan sulfate proteoglycans in infection of human adenovirus serotype 3 and 35.
PLoS Pathog. 4 (10): e1000189. -
Iwase, H. et al. (2014) Regulation of human platelet aggregation by genetically modified pig endothelial cells and thrombin inhibition.
Xenotransplantation. 21 (1): 72-83. -
Sweigard, J.H. et al. (2011) Adenovirus-mediated delivery of CD46 attenuates the alternative complement pathway on RPE: implications for age-related macular degeneration.
Gene Ther. 18 (6): 613-21.
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