CD44v6 antibody | VFF-18
Mouse anti Human CD44v6 antibody, clone VFF-18 (MCA5950) used for the demonstration of CD44v6 expression in human head and neck cancer tissue samples by immunohistochemistry on formalin fixed paraffin embedded specimens.
Image caption:
Expression of CD44v6 and EGFR in primary human HNSCC samples. Tumor (T), normal epithelium (E), stroma (S). Dotted lines mark the tumor edge. Representative samples from 7 (CD44v6) or 5 (EGFR) independent tumors. Scale bars indicate 1000 μm (overview) and 100 μm (zoom).
From: Odenthal J, Rijpkema M, Bos D, Wagena E, Croes H, Grenman R, Boerman O, Takes R, Friedl P.
Targeting CD44v6 for fluorescence-guided surgery in head and neck squamous cell carcinoma.
Sci Rep. 2018 Jul 11;8(1):10467.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD44v6, clone VFF-18, specifically recognizes a centrally located 14mer epitope encoded by amino acid residues 18-31, within variant exon 6 (v6) of human CD44 (Reinisch et al, 1998). CD44 is a member of a polymorphic family of transmembrane glycoproteins, which exists as a broadly expressed ~80-90 kDa standard form (CD44s), and also as numerous splice variants, due to extensive alternative splicing and post-translational modifications.
Many functional properties of CD44s have been determined, including cell adhesion, lymphocyte activation, signal transduction, haematopoiesis, recirculation and homing, and CD44v6 has been particularly implicated in leukocyte to endothelial cell attachment and rolling, as well as homing to sites of inflammation and peripheral organs.
As well as its role as a receptor for hyaluronic acid (HA), CD44s also interacts with other ligands including matrix metalloproteinases (MMPs), fibronectin and collagens, and has been linked with cancer of the breast, colon, stomach and squamous cell carcinomas (HNSCC) of the head and neck, as well as being a prognostic marker for non-Hodgkin's lymphoma.
The variant isoforms of CD44 (CD44v) are highly expressed on epithelial cells but not leukocytes, and their association with cell transformation and cancer is also well established, supported by numerous immunohistochemical studies including those looking at HNSCC. Studies looking at the role of CD44v6 in Crohn's disease, in which clone VFF-18 was generated and characterized, revealed that VFF-18 binds to CD44v6 with high affinity when compared with anti-CD44v6 clone 2F10, and is suitable for detecting CD44v6 in inflammatory bowel disease (IBD) (Reinisch et al. 1998).
Mouse anti Human CD44v6, clone VFF-18 is suitable for detecting CD44v6 in inflammatory bowel disease (IBD) (Reinisch et al. 1998).
Many functional properties of CD44s have been determined, including cell adhesion, lymphocyte activation, signal transduction, haematopoiesis, recirculation and homing, and CD44v6 has been particularly implicated in leukocyte to endothelial cell attachment and rolling, as well as homing to sites of inflammation and peripheral organs.
As well as its role as a receptor for hyaluronic acid (HA), CD44s also interacts with other ligands including matrix metalloproteinases (MMPs), fibronectin and collagens, and has been linked with cancer of the breast, colon, stomach and squamous cell carcinomas (HNSCC) of the head and neck, as well as being a prognostic marker for non-Hodgkin's lymphoma.
The variant isoforms of CD44 (CD44v) are highly expressed on epithelial cells but not leukocytes, and their association with cell transformation and cancer is also well established, supported by numerous immunohistochemical studies including those looking at HNSCC. Studies looking at the role of CD44v6 in Crohn's disease, in which clone VFF-18 was generated and characterized, revealed that VFF-18 binds to CD44v6 with high affinity when compared with anti-CD44v6 clone 2F10, and is suitable for detecting CD44v6 in inflammatory bowel disease (IBD) (Reinisch et al. 1998).
Mouse anti Human CD44v6, clone VFF-18 is suitable for detecting CD44v6 in inflammatory bowel disease (IBD) (Reinisch et al. 1998).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Glutathione S Transferase (GST) fusion protein corresponding to the variable domain v6 of human CD44.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the P3X63Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
Applications of CD44v6 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/5 | |
| ELISA | |||
| Flow Cytometry | |||
| Immunohistology - Frozen | 1.0 | 2.0ug/ml | |
| Immunohistology - Paraffin 1 | 1.0 | 2.0ug/ml | |
| Western Blotting |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 or Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD44v6 antibody
-
Reinisch, W. et al. (1998) Poor diagnostic value of colonic CD44v6 expression and serum concentrations of its soluble form in the differentiation of ulcerative colitis from Crohn's disease.
Gut. 43 (3): 375-82. -
Foekens, J.A. et al. (1999) Prognostic value of CD44 variant expression in primary breast cancer.
Int J Cancer. 84 (3): 209-15. -
Adolf, G.R. and Patzelt, E. (1999) Monoclonal antibody against CD44v6
US patent pub No: US08750359 -
Odenthal, J. et al. (2018) Targeting CD44v6 for fluorescence-guided surgery in head and neck squamous cell carcinoma.
Sci Rep. 8 (1): 10467.
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