Mouse anti Human CD44 antibody, clone Bu52 recognizes the human CD44 cell surface antigen, a ~100 kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and of the breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. Mouse anti Human CD44 antibody, clone Bu52 recognizes an epitope that is common to all CD44 isoforms.
CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD44 antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD44 antibody
Takahashi, K. et al. (1996) Keratan sulfate modification of CD44 modulates adhesion to hyaluronate.
J Biol Chem. 271 (16): 9490-6.
Belitsos, P.C. et al. (1990) Homotypic cell aggregation induced by anti-CD44(Pgp-1) monoclonal antibodies and related to CD44(Pgp-1) expression.
J Immunol. 144 (5): 1661-70.
Katz, E. et al. (2012) Targeting of Rac GTPases blocks the spread of intact human breast cancer.
Oncotarget. 3 (6): 608-19.
Noda, S. et al. (2019) Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.
Sci Rep. 9 (1): 5430.
Hanke-Roos, M. et al. (2017) CD44 mediates the catch-bond activated rolling of HEPG2Iso epithelial cancer cells on hyaluronan.
Cell Adh Migr. 11 (5-6): 476-87.
Muerza-Cascante, M.L. et al. (2017) Endosteal-like extracellular matrix expression on melt electrospun written scaffolds.
Acta Biomater. 52: 145-58.
Thorne, R.F. et al. (2020) Evaluating nuclear translocation of surface receptors: recommendations arising from analysis of CD44.
Histochem Cell Biol. 153 (2): 77-87.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra