CD44 antibody | Bu52
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Mouse anti Human CD44
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD44 antibody, clone Bu52 recognizes the human CD44 cell surface antigen, a ~100 kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and of the breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. Mouse anti Human CD44 antibody, clone Bu52 recognizes an epitope that is common to all CD44 isoforms.
CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3)
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1||1/100||1/200|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD44 antibody
Belitsos, P.C. et al. (1990) Homotypic cell aggregation induced by anti-CD44(Pgp-1) monoclonal antibodies and related to CD44(Pgp-1) expression.
J Immunol. 144 (5): 1661-70.
Katz, E. et al. (2012) Targeting of Rac GTPases blocks the spread of intact human breast cancer.
Oncotarget. 3 (6): 608-19.
Muerza-Cascante, M.L. et al. (2017) Endosteal-like extracellular matrix expression on melt electrospun written scaffolds.
Acta Biomater. 52: 145-58.
Hanke-Roos, M. et al. (2017) CD44 mediates the catch-bond activated rolling of HEPG2Iso epithelial cancer cells on hyaluronan.
Cell Adh Migr. 11 (5-6): 476-87.
Noda, S. et al. (2019) Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.
Sci Rep. 9 (1): 5430.
Thorne, R.F. et al. (2020) Evaluating nuclear translocation of surface receptors: recommendations arising from analysis of CD44.
Histochem Cell Biol. 153 (2): 77-87.
Orikasa, S. et al. (2022) Hypoxia-inducible factor 1α induces osteo/odontoblast differentiation of human dental pulp stem cells via Wnt/β-catenin transcriptional cofactor BCL9.
Sci Rep. 12 (1): 682.
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