CD43 antibody | DFT-1

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Mouse anti Human CD43:FITC

Mouse anti Human CD43

Mouse anti Human CD43:RPE

Product Type
Monoclonal Antibody
Clone
DFT-1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA555F F 0.1 mg
MCA555 C F IF P 0.2 mg
MCA555PET F 25 Tests
MCA555PE F 100 Tests
Mouse anti Human CD43 antibody, clone DFT-1 recognizes human CD43 also known as leukosialin or sialophorin. CD43 is a 381 amino acid single pass type I transmembrane glycoprotein present on the cell surface of multiple cell types including thymocytes, T-lymphocytes, neutrophils, plasma cells and platelets. It is lacking on the surface of most B-lymphocytes. CD34 is a major membrane sialoglycoprotein expressed on T lymphocytes as two predominant glycoforms, a 115kDa form present on all T-cells and a 130kDa form indicative of T-cell activation (Barren et al. 1997) and acts as a T-cell counter-receptor for CD169/Siglec-1 (van den Berg et al. 2001). Clone DFT-1 binds to both glycoforms of human CD43 (Kyoizumi et al. 2004)

CD34 expression is deficient and/or defective in patients suffering from the X-linked chromosomal immunodeficiency condition Wiskott-Aldrich syndrome and acts as a ligand for CD54 (Rosenstein et al. 1991).

Mouse anti human CD43, clone DFT-1 has been used successfully for the evaluation of CD43 expression in patients with Myelodysplastic syndromes (Kyriakou et al. 2006).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 25 Tests
Reconstitute in 0.25 ml disilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
KG-1 cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P16150 Related reagents
Entrez Gene
SPN Related reagents
GO Terms
GO:0042742 defense response to bacterium
GO:0007596 blood coagulation
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006955 immune response
GO:0006968 cellular defense response
GO:0007162 negative regulation of cell adhesion
GO:0007163 establishment or maintenance of cell polarity
GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
GO:0050900 leukocyte migration
GO:0051635 bacterial cell surface binding
Regulatory
For research purposes only

Applications of CD43 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/100
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1/10 1/100
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 100ul of whole blood or 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections however, staining of paraffin embedded formalin fixed tissues may be improved in some cases by enzyme pretreatment using Trypsin.
Histology Positive Control Tissue
Tonsil

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD43 antibody

  1. Stross, W.P. et al. (1989) Molecule detected in formalin fixed tissue by antibodies MT1, DF-T1, and L60 (Leu-22) corresponds to CD43 antigen.
    J Clin Pathol. 42 (9): 953-61.
  2. Kojima N et al. (1994) Role of cell surface O-linked oligosaccharides in adhesion of HL60 cells to fibronectin: regulation of integrin-dependent cell adhesion by O-linked oligosaccharide elongation.
    Exp Cell Res. 214 (2): 537-42.
  3. Anzai, N. et al. (1999) Modulation of integrin function in hematopoietic progenitor cells by CD43 engagement: possible involvement of protein tyrosine kinase and phospholipase C-gamma.
    Blood. 93 (10): 3317-26.
  4. Matsuo, A. et al. (1996) Expression of CD43 in human microglia and its downregulation in Alzheimer's disease.
    J Neuroimmunol. 71 (1-2): 81-6.
  5. Nonomura, C. et al. (2008) CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1.
    Cancer Res. 68 (3): 790-9.